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Protein

Protein kinase C-like 1B

Gene

pkc-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Involved in neuropeptide secretion in motor axons. Likely to act via the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway in the signaling response to various sensory neurons; temperature, odor, taste, and osmolality. Its role in regulation differs depending on the neuron in which it is acting; thermosensation in AFD neurons, osmolality in ASH neurons, olfactory perception in AWA and AWC neurons. Promotes dauer formation mediated by the insulin/IGF pathway. Required for resistance to antimitotic toxins.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei407 – 4071ATPPROSITE-ProRule annotation
Active sitei502 – 5021Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri170 – 22051Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 29851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi384 – 3929ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase C activity Source: WormBase
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • associative learning Source: UniProtKB
  • chemosensory behavior Source: UniProtKB
  • chemotaxis Source: WormBase
  • dauer larval development Source: UniProtKB
  • dense core granule exocytosis Source: WormBase
  • insulin-like growth factor receptor signaling pathway Source: UniProtKB
  • MAPK cascade Source: UniProtKB
  • negative regulation of response to drug Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB
  • neurotransmitter secretion Source: WormBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of positive chemotaxis Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • regulation of chemotaxis Source: UniProtKB
  • regulation of locomotion Source: WormBase
  • response to salt Source: UniProtKB
  • sensory perception of chemical stimulus Source: UniProtKB
  • sensory perception of touch Source: UniProtKB
  • sensory processing Source: UniProtKB
  • synaptic vesicle exocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13. 1045.
ReactomeiR-CEL-114508. Effects of PIP2 hydrolysis.
R-CEL-1489509. DAG and IP3 signaling.
R-CEL-2029485. Role of phospholipids in phagocytosis.
SignaLinkiP34885.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C-like 1B (EC:2.7.11.13)
Short name:
PKC1B
Gene namesi
Name:pkc-1
Synonyms:kin-13, pkc-1b, ttx-4
ORF Names:F57F5.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF57F5.5a; CE29092; WBGene00004032; pkc-1.
F57F5.5b; CE46445; WBGene00004032; pkc-1.

Subcellular locationi

GO - Cellular componenti

  • cell Source: UniProtKB
  • cytoskeleton Source: UniProtKB-SubCell
  • cytosol Source: GOC
  • intracellular Source: GO_Central
  • membrane Source: UniProtKB-SubCell
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Attenuated response to nose touch stimulation. Defects in salt attraction. Disrupted chemotaxis. Hyperactivation of AFD thermosensory neurons but inactivation of the ASH neurons. Reduced dauer formation. Protection from hemiasterlin toxicity by carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP) greatly reduced. Reduced neuropeptide secretion.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Protein kinase C-like 1BPRO_0000055735Add
BLAST

Proteomic databases

EPDiP34885.
PaxDbiP34885.

PTM databases

iPTMnetiP34885.

Expressioni

Tissue specificityi

Expressed selectively in neurons that receive, transmit and process environmental signals.

Gene expression databases

ExpressionAtlasiP34885. baseline.

Interactioni

Protein-protein interaction databases

BioGridi44672. 2 interactions.
DIPiDIP-27455N.
MINTiMINT-1048585.
STRINGi6239.F57F5.5a.2.

Structurei

3D structure databases

ProteinModelPortaliP34885.
SMRiP34885. Positions 1-128, 164-301, 316-703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9898C2PROSITE-ProRule annotationAdd
BLAST
Domaini378 – 638261Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini639 – 70769AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri170 – 22051Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 29851Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
InParanoidiP34885.
PhylomeDBiP34885.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P34885-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLFTGTVRVR VLEARQLRPT EWSRRFRQDE AATAAIDSYV NVDWDEYHIG
60 70 80 90 100
KTQVRPKTNE PRWNEEFTAS GVHQGKAIGF SVFHSCVMPP DDFVANTRIA
110 120 130 140 150
FDQLKIGSAN DIWVDLEPHG QLHVVVEMHG TNVEDVHSHN KTRVFKERTN
160 170 180 190 200
AFNDRQRRGA MRRKIHEVTG HKFMALFLRQ PTFCAHCKEF IWGIGKQGYQ
210 220 230 240 250
CQICTVVVHK RCHEDVVWKC PGNKADAVEE LGKEIQETGA GRFNINMPHR
260 270 280 290 300
FSVHSYKRPT FCDHCGSMLY GLINQGLQCS TCKLNVHKRC QRNVANNCGI
310 320 330 340 350
NAKQMAAELA QLGLTGDKMS IRSKKKPSIM TDTSTDISGS SNSENSGYLQ
360 370 380 390 400
QISEDDSGTT SSRSASKVPG GTLSIHDFTF MKVLGKGSFG KVMLAERKGT
410 420 430 440 450
DEVYAIKILK KDVIVQDDDV ECTMCEKRIL SLAAKHPFLT ALHSSFQTSD
460 470 480 490 500
RLFFVMEYVN GGDLMFQIQR ARKFDESRAR FYAAEVTCAL QFLHRNDVIY
510 520 530 540 550
RDLKLDNILL DAEGHCRLAD FGMCKEGINK DNLTSTFCGT PDYIAPEILQ
560 570 580 590 600
EMEYGVSVDW WALGVLMYEM MAGQPPFEAD NEDDLFEAIL NDDVLYPVWL
610 620 630 640 650
SKEAVNILKA FMTKNAGKRL GCVVSQGGED AIRAHPFFRE IDWDALESRQ
660 670 680 690 700
VKPPFKPKIK SKRDANNFDS DFTKEEPVLT PSDPAVVRAI NQDEFRGFSF

INPHFTY
Length:707
Mass (Da):80,151
Last modified:February 16, 2004 - v2
Checksum:i08C1BD1B7A911B49
GO
Isoform b (identifier: P34885-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-134: LFTGTVRVRV...VVEMHGTNVE → SFDCLVYDEY...QDSTSSSSFH

Note: No experimental confirmation available.
Show »
Length:620
Mass (Da):70,211
Checksum:i9141032E4F657ADB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1812QP → HA in AAA18259 (PubMed:8132661).Curated
Sequence conflicti544 – 5441I → T in AAA18259 (PubMed:8132661).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 134133LFTGT…GTNVE → SFDCLVYDEYAPSSKSRNSS NKAKVKKKNSWGFWVKQDST SSSSFH in isoform b. CuratedVSP_044041Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00181 mRNA. Translation: AAA18259.1.
U00965 Genomic DNA. Translation: AAA17996.1.
Z75953, Z81497 Genomic DNA. Translation: CAB00101.2.
Z75953 Genomic DNA. Translation: CCD31083.1.
PIRiA53530.
T22856.
RefSeqiNP_001256394.1. NM_001269465.1. [P34885-2]
NP_001256395.1. NM_001269466.1. [P34885-1]
UniGeneiCel.19762.

Genome annotation databases

EnsemblMetazoaiF57F5.5a; F57F5.5a; WBGene00004032. [P34885-1]
GeneIDi179649.
UCSCiF57F5.5. c. elegans. [P34885-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00181 mRNA. Translation: AAA18259.1.
U00965 Genomic DNA. Translation: AAA17996.1.
Z75953, Z81497 Genomic DNA. Translation: CAB00101.2.
Z75953 Genomic DNA. Translation: CCD31083.1.
PIRiA53530.
T22856.
RefSeqiNP_001256394.1. NM_001269465.1. [P34885-2]
NP_001256395.1. NM_001269466.1. [P34885-1]
UniGeneiCel.19762.

3D structure databases

ProteinModelPortaliP34885.
SMRiP34885. Positions 1-128, 164-301, 316-703.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44672. 2 interactions.
DIPiDIP-27455N.
MINTiMINT-1048585.
STRINGi6239.F57F5.5a.2.

PTM databases

iPTMnetiP34885.

Proteomic databases

EPDiP34885.
PaxDbiP34885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF57F5.5a; F57F5.5a; WBGene00004032. [P34885-1]
GeneIDi179649.
UCSCiF57F5.5. c. elegans. [P34885-1]

Organism-specific databases

CTDi179649.
WormBaseiF57F5.5a; CE29092; WBGene00004032; pkc-1.
F57F5.5b; CE46445; WBGene00004032; pkc-1.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
InParanoidiP34885.
PhylomeDBiP34885.

Enzyme and pathway databases

BRENDAi2.7.11.13. 1045.
ReactomeiR-CEL-114508. Effects of PIP2 hydrolysis.
R-CEL-1489509. DAG and IP3 signaling.
R-CEL-2029485. Role of phospholipids in phagocytosis.
SignaLinkiP34885.

Miscellaneous databases

PROiP34885.

Gene expression databases

ExpressionAtlasiP34885. baseline.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of a novel, neuronal protein kinase C (PKC1B) from Caenorhabditis elegans. PKC1B is expressed selectively in neurons that receive, transmit, and process environmental signals."
    Land M., Islas-Trejo A., Freedman J.H., Rubin C.S.
    J. Biol. Chem. 269:9234-9244(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 (ISOFORM A).
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Diverse regulation of sensory signaling by C. elegans nPKC-epsilon/eta TTX-4."
    Okochi Y., Kimura K.D., Ohta A., Mori I.
    EMBO J. 24:2127-2137(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: IK105.
  4. "PKC-1 regulates secretion of neuropeptides."
    Sieburth D., Madison J.M., Kaplan J.M.
    Nat. Neurosci. 10:49-57(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: IK130.
  5. "Reversal of salt preference is directed by the insulin/PI3K and Gq/PKC signaling in Caenorhabditis elegans."
    Adachi T., Kunitomo H., Tomioka M., Ohno H., Okochi Y., Mori I., Iino Y.
    Genetics 186:1309-1319(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: IK130.
  6. "Mitochondrial dysfunction confers resistance to multiple drugs in Caenorhabditis elegans."
    Zubovych I.O., Straud S., Roth M.G.
    Mol. Biol. Cell 21:956-969(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: IK105.
  7. "pkc-1 regulates daf-2 insulin/IGF signalling-dependent control of dauer formation in Caenorhabditis elegans."
    Monje J.M., Brokate-Llanos A.M., Perez-Jimenez M.M., Fidalgo M.A., Munoz M.J.
    Aging Cell 10:1021-1031(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: IK130.
  8. "PKC-1 acts with the ERK MAPK signaling pathway to regulate Caenorhabditis elegans mechanosensory response."
    Hyde R., Corkins M.E., Somers G.A., Hart A.C.
    Genes Brain Behav. 10:286-298(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: RB781.

Entry informationi

Entry nameiKPC1B_CAEEL
AccessioniPrimary (citable) accession number: P34885
Secondary accession number(s): G3MU31, Q20953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 16, 2004
Last modified: July 6, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.