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Protein

Protein kinase C-like 1B

Gene

pkc-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Involved in neuropeptide secretion in motor axons. Likely to act via the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway in the signaling response to various sensory neurons; temperature, odor, taste, and osmolality. Its role in regulation differs depending on the neuron in which it is acting; thermosensation in AFD neurons, osmolality in ASH neurons, olfactory perception in AWA and AWC neurons. Promotes dauer formation mediated by the insulin/IGF pathway. Required for resistance to antimitotic toxins.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei407ATPPROSITE-ProRule annotation1
Active sitei502Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri170 – 220Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri248 – 298Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi384 – 392ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase C activity Source: WormBase
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • associative learning Source: UniProtKB
  • chemosensory behavior Source: UniProtKB
  • chemotaxis Source: WormBase
  • dauer larval development Source: UniProtKB
  • dense core granule exocytosis Source: WormBase
  • insulin-like growth factor receptor signaling pathway Source: UniProtKB
  • MAPK cascade Source: UniProtKB
  • negative regulation of response to drug Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB
  • neurotransmitter secretion Source: WormBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of positive chemotaxis Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • regulation of chemotaxis Source: UniProtKB
  • regulation of locomotion Source: WormBase
  • response to salt Source: UniProtKB
  • sensory perception of chemical stimulus Source: UniProtKB
  • sensory perception of touch Source: UniProtKB
  • sensory processing Source: UniProtKB
  • synaptic vesicle exocytosis Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13 1045
ReactomeiR-CEL-114508 Effects of PIP2 hydrolysis
R-CEL-1489509 DAG and IP3 signaling
R-CEL-2029485 Role of phospholipids in phagocytosis
SignaLinkiP34885

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C-like 1B (EC:2.7.11.13)
Short name:
PKC1B
Gene namesi
Name:pkc-1
Synonyms:kin-13, pkc-1b, ttx-4
ORF Names:F57F5.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF57F5.5a ; CE29092 ; WBGene00004032 ; pkc-1
F57F5.5b ; CE46445 ; WBGene00004032 ; pkc-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Attenuated response to nose touch stimulation. Defects in salt attraction. Disrupted chemotaxis. Hyperactivation of AFD thermosensory neurons but inactivation of the ASH neurons. Reduced dauer formation. Protection from hemiasterlin toxicity by carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP) greatly reduced. Reduced neuropeptide secretion.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557351 – 707Protein kinase C-like 1BAdd BLAST707

Proteomic databases

EPDiP34885
PaxDbiP34885
PeptideAtlasiP34885

PTM databases

iPTMnetiP34885

Expressioni

Tissue specificityi

Expressed selectively in neurons that receive, transmit and process environmental signals.

Gene expression databases

BgeeiWBGene00004032
ExpressionAtlasiP34885 baseline and differential

Interactioni

Protein-protein interaction databases

BioGridi44672, 2 interactors
DIPiDIP-27455N
STRINGi6239.F57F5.5a.2

Structurei

3D structure databases

ProteinModelPortaliP34885
SMRiP34885
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 98C2PROSITE-ProRule annotationAdd BLAST98
Domaini378 – 638Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini639 – 707AGC-kinase C-terminalAdd BLAST69

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri170 – 220Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri248 – 298Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00820000126964
HOGENOMiHOG000233022
InParanoidiP34885
PhylomeDBiP34885

Family and domain databases

CDDicd00029 C1, 2 hits
cd05591 STKc_nPKC_epsilon, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR034669 nPKC_epsilon
IPR002219 PE/DAG-bd
IPR017892 Pkinase_C
IPR014376 Prot_kin_PKC_delta
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00168 C2, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
PIRSFiPIRSF000551 PKC_delta, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00239 C2, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50004 C2, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P34885-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLFTGTVRVR VLEARQLRPT EWSRRFRQDE AATAAIDSYV NVDWDEYHIG
60 70 80 90 100
KTQVRPKTNE PRWNEEFTAS GVHQGKAIGF SVFHSCVMPP DDFVANTRIA
110 120 130 140 150
FDQLKIGSAN DIWVDLEPHG QLHVVVEMHG TNVEDVHSHN KTRVFKERTN
160 170 180 190 200
AFNDRQRRGA MRRKIHEVTG HKFMALFLRQ PTFCAHCKEF IWGIGKQGYQ
210 220 230 240 250
CQICTVVVHK RCHEDVVWKC PGNKADAVEE LGKEIQETGA GRFNINMPHR
260 270 280 290 300
FSVHSYKRPT FCDHCGSMLY GLINQGLQCS TCKLNVHKRC QRNVANNCGI
310 320 330 340 350
NAKQMAAELA QLGLTGDKMS IRSKKKPSIM TDTSTDISGS SNSENSGYLQ
360 370 380 390 400
QISEDDSGTT SSRSASKVPG GTLSIHDFTF MKVLGKGSFG KVMLAERKGT
410 420 430 440 450
DEVYAIKILK KDVIVQDDDV ECTMCEKRIL SLAAKHPFLT ALHSSFQTSD
460 470 480 490 500
RLFFVMEYVN GGDLMFQIQR ARKFDESRAR FYAAEVTCAL QFLHRNDVIY
510 520 530 540 550
RDLKLDNILL DAEGHCRLAD FGMCKEGINK DNLTSTFCGT PDYIAPEILQ
560 570 580 590 600
EMEYGVSVDW WALGVLMYEM MAGQPPFEAD NEDDLFEAIL NDDVLYPVWL
610 620 630 640 650
SKEAVNILKA FMTKNAGKRL GCVVSQGGED AIRAHPFFRE IDWDALESRQ
660 670 680 690 700
VKPPFKPKIK SKRDANNFDS DFTKEEPVLT PSDPAVVRAI NQDEFRGFSF

INPHFTY
Length:707
Mass (Da):80,151
Last modified:February 16, 2004 - v2
Checksum:i08C1BD1B7A911B49
GO
Isoform b (identifier: P34885-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-134: LFTGTVRVRV...VVEMHGTNVE → SFDCLVYDEY...QDSTSSSSFH

Note: No experimental confirmation available.
Show »
Length:620
Mass (Da):70,211
Checksum:i9141032E4F657ADB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180 – 181QP → HA in AAA18259 (PubMed:8132661).Curated2
Sequence conflicti544I → T in AAA18259 (PubMed:8132661).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0440412 – 134LFTGT…GTNVE → SFDCLVYDEYAPSSKSRNSS NKAKVKKKNSWGFWVKQDST SSSSFH in isoform b. CuratedAdd BLAST133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00181 mRNA Translation: AAA18259.1
U00965 Genomic DNA Translation: AAA17996.1
Z75953, Z81497 Genomic DNA Translation: CAB00101.2
Z75953 Genomic DNA Translation: CCD31083.1
PIRiA53530
T22856
RefSeqiNP_001256394.1, NM_001269465.1 [P34885-2]
NP_001256395.1, NM_001269466.1 [P34885-1]
UniGeneiCel.19762

Genome annotation databases

EnsemblMetazoaiF57F5.5a; F57F5.5a; WBGene00004032 [P34885-1]
GeneIDi179649
UCSCiF57F5.5 c. elegans [P34885-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKPC1B_CAEEL
AccessioniPrimary (citable) accession number: P34885
Secondary accession number(s): G3MU31, Q20953
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 16, 2004
Last modified: May 23, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families
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