P34885 (KPC1B_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase C-like 1B
Short name=PKC1B
EC=2.7.11.13

Gene names

Name:	pkc-1
Synonyms:	kin-13, pkc-1b
ORF Names:	F57F5.5

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Membrane; Peripheral membrane protein. Cytoplasm › cytoskeleton. Note: Associated with membranes and the cytoskeleton.
Tissue specificity	Expressed selectively in neurons that receive, transmit and process environmental signals.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Membrane
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Protein kinase C-like 1B		PRO_0000055735
Regions
	Domain	1 – 98	98	C2
	Domain	378 – 638	261	Protein kinase
	Domain	639 – 707	69	AGC-kinase C-terminal
	Zinc finger	170 – 220	51	Phorbol-ester/DAG-type 1
	Zinc finger	248 – 298	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	384 – 392	9	ATP By similarity
Sites
	Active site	502	1	Proton acceptor By similarity
	Binding site	407	1	ATP By similarity
Experimental info
	Sequence conflict	180 – 181	2	QP → HA in AAA18259. Ref.1
	Sequence conflict	544	1	I → T in AAA18259. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34885 [UniParc]. Last modified February 16, 2004. Version 2. Checksum: 08C1BD1B7A911B49	FASTA	707	80,151
	10 20 30 40 50 60 MLFTGTVRVR VLEARQLRPT EWSRRFRQDE AATAAIDSYV NVDWDEYHIG KTQVRPKTNE 70 80 90 100 110 120 PRWNEEFTAS GVHQGKAIGF SVFHSCVMPP DDFVANTRIA FDQLKIGSAN DIWVDLEPHG 130 140 150 160 170 180 QLHVVVEMHG TNVEDVHSHN KTRVFKERTN AFNDRQRRGA MRRKIHEVTG HKFMALFLRQ 190 200 210 220 230 240 PTFCAHCKEF IWGIGKQGYQ CQICTVVVHK RCHEDVVWKC PGNKADAVEE LGKEIQETGA 250 260 270 280 290 300 GRFNINMPHR FSVHSYKRPT FCDHCGSMLY GLINQGLQCS TCKLNVHKRC QRNVANNCGI 310 320 330 340 350 360 NAKQMAAELA QLGLTGDKMS IRSKKKPSIM TDTSTDISGS SNSENSGYLQ QISEDDSGTT 370 380 390 400 410 420 SSRSASKVPG GTLSIHDFTF MKVLGKGSFG KVMLAERKGT DEVYAIKILK KDVIVQDDDV 430 440 450 460 470 480 ECTMCEKRIL SLAAKHPFLT ALHSSFQTSD RLFFVMEYVN GGDLMFQIQR ARKFDESRAR 490 500 510 520 530 540 FYAAEVTCAL QFLHRNDVIY RDLKLDNILL DAEGHCRLAD FGMCKEGINK DNLTSTFCGT 550 560 570 580 590 600 PDYIAPEILQ EMEYGVSVDW WALGVLMYEM MAGQPPFEAD NEDDLFEAIL NDDVLYPVWL 610 620 630 640 650 660 SKEAVNILKA FMTKNAGKRL GCVVSQGGED AIRAHPFFRE IDWDALESRQ VKPPFKPKIK 670 680 690 700 SKRDANNFDS DFTKEEPVLT PSDPAVVRAI NQDEFRGFSF INPHFTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of a novel, neuronal protein kinase C (PKC1B) from Caenorhabditis elegans. PKC1B is expressed selectively in neurons that receive, transmit, and process environmental signals." Land M., Islas-Trejo A., Freedman J.H., Rubin C.S. J. Biol. Chem. 269:9234-9244(1994) [PubMed: 8132661] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00181 mRNA. Translation: AAA18259.1. U00965 Genomic DNA. Translation: AAA17996.1. Z75953, Z81497 Genomic DNA. Translation: CAB00101.2. Z81497, Z75953 Genomic DNA. Translation: CAC70080.1.
PIR	A53530. T22856.
RefSeq	NP_506014.1. NM_073613.5.
UniGene	Cel.19762.
3D structure databases
ProteinModelPortal	P34885.
SMR	P34885. Positions 1-129, 169-222, 245-301, 377-705.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27455N.
MINT	MINT-1048585.
STRING	P34885.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F57F5.5.1; F57F5.5.1; F57F5.5. F57F5.5.2; F57F5.5.2; F57F5.5.
GeneID	179649.
KEGG	cel:F57F5.5.
UCSC	F57F5.5. c. elegans.
Organism-specific databases
CTD	179649.
WormBase	F57F5.5; CE29092; WBGene00004032; pkc-1.
Phylogenomic databases
eggNOG	meNOG04868.
GeneTree	EMGT00050000001097.
HOGENOM	HBG755340.
InParanoid	P34885.
OMA	KNVANNC.
PhylomeDB	P34885.
Enzyme and pathway databases
BRENDA	2.7.11.13. 1045.
Gene expression databases
ArrayExpress	P34885.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR020454. DAG/PE-bd. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR014376. Prot_kin_PKC_delta. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K06068.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000551. PKC_delta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	906294.

Entry information

Entry name

KPC1B_CAEEL

Accession

Primary (citable) accession number: P34885
Secondary accession number(s): Q20953

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 16, 2004
Last modified:	November 16, 2011
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project