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Protein

Macrophage migration inhibitory factor

Gene

Mif

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Miscellaneous

MIF tautomerase inhibitors improve survival in case of sepsis.

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.1 Publication
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Proton acceptor; via imino nitrogen1
Binding sitei33Substrate1
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei98Substrate1

GO - Molecular functioni

  • chemoattractant activity Source: MGI
  • cytokine activity Source: BHF-UCL
  • cytokine receptor binding Source: MGI
  • dopachrome isomerase activity Source: UniProtKB
  • identical protein binding Source: MGI
  • phenylpyruvate tautomerase activity Source: MGI
  • protease binding Source: BHF-UCL
  • signaling receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionCytokine, Isomerase
Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

BRENDAi5.3.2.1 3474
ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
Delayed early response protein 6
Short name:
DER6
Glycosylation-inhibiting factor
Short name:
GIF
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:Mif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96982 Mif

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2P → G: Loss of tautomerase activity, reduced activation of intracellular signaling pathways, and reduced interaction with CXCR2 and COPS5. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL1926491

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001580662 – 115Macrophage migration inhibitory factorAdd BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysine; alternateCombined sources1
Modified residuei78N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP34884
PaxDbiP34884
PeptideAtlasiP34884
PRIDEiP34884
TopDownProteomicsiP34884

PTM databases

iPTMnetiP34884
PhosphoSitePlusiP34884
SwissPalmiP34884

Expressioni

Gene expression databases

BgeeiENSMUSG00000033307
CleanExiMM_MIF
ExpressionAtlasiP34884 baseline and differential
GenevisibleiP34884 MM

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain.By similarity2 Publications

GO - Molecular functioni

  • chemoattractant activity Source: MGI
  • cytokine activity Source: BHF-UCL
  • cytokine receptor binding Source: MGI
  • identical protein binding Source: MGI
  • protease binding Source: BHF-UCL
  • signaling receptor binding Source: MGI

Protein-protein interaction databases

BioGridi201418, 1 interactor
IntActiP34884, 4 interactors
MINTiP34884
STRINGi10090.ENSMUSP00000041149

Chemistry databases

BindingDBiP34884

Structurei

Secondary structure

1115
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Helixi12 – 14Combined sources3
Helixi19 – 31Combined sources13
Helixi35 – 37Combined sources3
Beta strandi39 – 43Combined sources5
Beta strandi47 – 50Combined sources4
Beta strandi58 – 66Combined sources9
Helixi70 – 88Combined sources19
Helixi92 – 94Combined sources3
Beta strandi95 – 101Combined sources7
Helixi104 – 106Combined sources3
Beta strandi107 – 109Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MFFX-ray2.00A/B/C2-115[»]
1MFIX-ray1.80A/B/C2-115[»]
2GDGX-ray1.45A/B/C2-115[»]
ProteinModelPortaliP34884
SMRiP34884
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34884

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiKOG1759 Eukaryota
ENOG41122MF LUCA
GeneTreeiENSGT00910000144267
HOGENOMiHOG000112325
HOVERGENiHBG003240
InParanoidiP34884
KOiK07253
OMAiPDRIYIN
OrthoDBiEOG091G0ZNJ
PhylomeDBiP34884
TreeFamiTF313853

Family and domain databases

Gene3Di3.30.429.10, 1 hit
InterProiView protein in InterPro
IPR001398 Macrophage_inhib_fac
IPR019829 Macrophage_inhib_fac_CS
IPR014347 Tautomerase/MIF_sf
PANTHERiPTHR11954 PTHR11954, 1 hit
PfamiView protein in Pfam
PF01187 MIF, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004816 Macrophage_inhib_fac, 1 hit
SUPFAMiSSF55331 SSF55331, 1 hit
PROSITEiView protein in PROSITE
PS01158 MIF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF
60 70 80 90 100
SGTNDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY
110
DMNAANVGWN GSTFA
Length:115
Mass (Da):12,504
Last modified:January 23, 2007 - v2
Checksum:i92D207B81B149945
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23048 mRNA Translation: CAA80583.1
U19825 Genomic DNA Translation: AAA91637.1
L10613 mRNA Translation: AAA37693.1
U20156 Genomic DNA Translation: AAA91638.1
L39357 Genomic DNA Translation: AAA74321.1
BC024895 mRNA Translation: AAH24895.1
BC086928 mRNA Translation: AAH86928.1
L07607 mRNA Translation: AAA37111.1
CCDSiCCDS23934.1
PIRiA44499
RefSeqiNP_034928.1, NM_010798.2
UniGeneiMm.2326

Genome annotation databases

EnsembliENSMUST00000038169; ENSMUSP00000041149; ENSMUSG00000033307
GeneIDi17319
KEGGimmu:17319
UCSCiuc007ftc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMIF_MOUSE
AccessioniPrimary (citable) accession number: P34884
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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