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P34883 (MTAB_SYNP2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase AquI subunit beta

Short name=M.AquI subunit beta
Short name=M.AquIB
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase AquI subunit beta
Gene names
Name:aquIMB
Ordered Locus Names:SYNPCC7002_A1188.1
OrganismSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum) [Complete proteome] [HAMAP]
Taxonomic identifier32049 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length139 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the AquI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Domain

Corresponds to the C-terminal half of the enzymatic domain.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 139139Modification methylase AquI subunit beta
PRO_0000087855

Regions

Domain1 – 135135SAM-dependent MTase C5-type

Sequences

Sequence LengthMass (Da)Tools
P34883 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 8550B7E0194CE006

FASTA13915,760
        10         20         30         40         50         60 
MDIKNVHIKN HEQTAHAPST LEKIRKVKQG GKLSEQTKTF GSTYRRLDPN QPSPTVTRSG 

        70         80         90        100        110        120 
YRDFIHPFED RMLTVRELAC LQTFPLDWEF TGTRLDSYSS KRKVTMTQFG QVGNAVPPLL 

       130 
AEAVAKAVSE QLLDVIDEK 

« Hide

References

« Hide 'large scale' references
[1]"Agmenellum quadruplicatum M.AquI, a novel modification methylase."
Karreman C., de Waard A.
J. Bacteriol. 172:266-272(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of Synechococcus sp. PCC 7002."
Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27264 / PCC 7002 / PR-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28051 Genomic DNA. Translation: AAA22068.1.
CP000951 Genomic DNA. No translation available.
RefSeqYP_003828901.1. NC_010475.1.

3D structure databases

ProteinModelPortalP34883.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3283. M.AquI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9488071.
KEGGsyp:SYNPCC7002_A2880.

Organism-specific databases

CMRSearch...

Enzyme and pathway databases

BioCycSSP32049:GKF7-1189-MONOMER.

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTAB_SYNP2
AccessionPrimary (citable) accession number: P34883
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries