Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Modification methylase AquI subunit beta

Gene

aquIMB

Organism
Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the AquI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSSP32049:GKF7-1189-MONOMER.

Protein family/group databases

REBASEi3283. M.AquI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AquI subunit beta (EC:2.1.1.37)
Short name:
M.AquI subunit beta
Short name:
M.AquIB
Alternative name(s):
Cytosine-specific methyltransferase AquI subunit beta
Gene namesi
Name:aquIMB
Ordered Locus Names:SYNPCC7002_A1188.1
OrganismiSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Taxonomic identifieri32049 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001688: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139Modification methylase AquI subunit betaPRO_0000087855Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Structurei

3D structure databases

ProteinModelPortaliP34883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 135135SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Domaini

Corresponds to the C-terminal half of the enzymatic domain.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34883-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDIKNVHIKN HEQTAHAPST LEKIRKVKQG GKLSEQTKTF GSTYRRLDPN
60 70 80 90 100
QPSPTVTRSG YRDFIHPFED RMLTVRELAC LQTFPLDWEF TGTRLDSYSS
110 120 130
KRKVTMTQFG QVGNAVPPLL AEAVAKAVSE QLLDVIDEK
Length:139
Mass (Da):15,760
Last modified:February 1, 1994 - v1
Checksum:i8550B7E0194CE006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28051 Genomic DNA. Translation: AAA22068.1.
CP000951 Genomic DNA. No translation available.
RefSeqiWP_012308460.1. NC_010475.1.
YP_003828901.1. NC_010475.1.

Genome annotation databases

GeneIDi9488071.
KEGGisyp:SYNPCC7002_A2880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28051 Genomic DNA. Translation: AAA22068.1.
CP000951 Genomic DNA. No translation available.
RefSeqiWP_012308460.1. NC_010475.1.
YP_003828901.1. NC_010475.1.

3D structure databases

ProteinModelPortaliP34883.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3283. M.AquI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9488071.
KEGGisyp:SYNPCC7002_A2880.

Enzyme and pathway databases

BioCyciSSP32049:GKF7-1189-MONOMER.

Family and domain databases

InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Agmenellum quadruplicatum M.AquI, a novel modification methylase."
    Karreman C., de Waard A.
    J. Bacteriol. 172:266-272(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of Synechococcus sp. PCC 7002."
    Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27264 / PCC 7002 / PR-6.

Entry informationi

Entry nameiMTAB_SYNP2
AccessioniPrimary (citable) accession number: P34883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.