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Protein

Modification methylase AquI subunit beta

Gene

aquIMB

Organism
Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the AquI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3283. M.AquI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AquI subunit beta (EC:2.1.1.37)
Short name:
M.AquI subunit beta
Short name:
M.AquIB
Alternative name(s):
Cytosine-specific methyltransferase AquI subunit beta
Gene namesi
Name:aquIMB
Ordered Locus Names:SYNPCC7002_A1188.1
OrganismiSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Taxonomic identifieri32049 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001688 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878551 – 139Modification methylase AquI subunit betaAdd BLAST139

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Protein-protein interaction databases

STRINGi32049.SYNPCC7002_A2880.

Structurei

3D structure databases

ProteinModelPortaliP34883.
SMRiP34883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 135SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST135

Domaini

Corresponds to the C-terminal half of the enzymatic domain.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P34883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIKNVHIKN HEQTAHAPST LEKIRKVKQG GKLSEQTKTF GSTYRRLDPN
60 70 80 90 100
QPSPTVTRSG YRDFIHPFED RMLTVRELAC LQTFPLDWEF TGTRLDSYSS
110 120 130
KRKVTMTQFG QVGNAVPPLL AEAVAKAVSE QLLDVIDEK
Length:139
Mass (Da):15,760
Last modified:February 1, 1994 - v1
Checksum:i8550B7E0194CE006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28051 Genomic DNA. Translation: AAA22068.1.
CP000951 Genomic DNA. No translation available.
RefSeqiWP_012308460.1. NC_010475.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMTAB_SYNP2
AccessioniPrimary (citable) accession number: P34883
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 12, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families