P34882 (MTAA_SYNP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase AquI subunit alpha
Short name=M.AquI subunit alpha
Short name=M.AquiA
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase AquI subunit alpha

Gene names

Name:	aquIMA
Synonyms:	dcm
Ordered Locus Names:	SYNPCC7002_A1188

Organism

Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum) [Complete proteome] [HAMAP]

Taxonomic identifier

32049 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the AquI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Domain	Corresponds to the N-terminal half of the enzymatic domain.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Modification methylase AquI subunit alpha		PRO_0000087854
Sites
	Active site	82	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34882 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FFF6B42B0997D82F	FASTA	248	27,161
	10 20 30 40 50 60 MEKKLISLFS GAGGMDIGFH AAGFSTAVAV EQDPSCCNTL RLNMPDTPVI EGDITSITTQ 70 80 90 100 110 120 VILEAAKVNP LEIDLVIGGP PCQSFSLAGK RMGMDDPRGM LVLEFLRVVR EALPKCFVME 130 140 150 160 170 180 NVKGMINWSK GKALEAIMTE ASQPIKYAGK EYKYAVSYHV LNAADFGVPQ FRERVFIVGN 190 200 210 220 230 240 RLGKTFQFPE PTHGPSNQAR QIDLFGKQLK PYKTVQDAIS TLPPATPPSA MALRVSQTIK DRIKNHGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Agmenellum quadruplicatum M.AquI, a novel modification methylase." Karreman C., de Waard A. J. Bacteriol. 172:266-272(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Synechococcus sp. PCC 7002." Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27264 / PCC 7002 / PR-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28051 Genomic DNA. Translation: AAA22067.1. CP000951 Genomic DNA. Translation: ACA99187.1.
RefSeq	YP_001734443.1. NC_010475.1.
3D structure databases
ProteinModelPortal	P34882.
ModBase	Search...
Protein-protein interaction databases
STRING	32049.SYNPCC7002_A1188.
Protein family/group databases
REBASE	3283. M.AquI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA99187; ACA99187; SYNPCC7002_A1188.
GeneID	6056178.
KEGG	syp:SYNPCC7002_A1188.
PATRIC	23816916. VBISynSp37135_1410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0270.
KO	K00558.
OMA	EWNCISA.
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTAA_SYNP2

Accession

Primary (citable) accession number: P34882
Secondary accession number(s): B1XKE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program