Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P34881

- DNMT1_ARATH

UniProt

P34881 - DNMT1_ARATH

Protein

DNA (cytosine-5)-methyltransferase 1

Gene

DMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. Required for proper patterns of CG DNA methylation in dividing cells. Required for MEA promoter methylation in seeds.7 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei1198 – 11981PROSITE-ProRule annotation

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    3. DNA binding Source: UniProtKB-KW
    4. methyltransferase activity Source: TAIR
    5. protein binding Source: TAIR

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. DNA mediated transformation Source: TAIR
    3. DNA methylation on cytosine within a CG sequence Source: TAIR
    4. maintenance of DNA methylation Source: TAIR
    5. negative regulation of flower development Source: TAIR
    6. regulation of gene expression by genetic imprinting Source: TAIR
    7. zygote asymmetric cytokinesis in embryo sac Source: TAIR

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT5G49160-MONOMER.

    Protein family/group databases

    REBASEi11752. M.AthMET1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase 1 (EC:2.1.1.37)
    Alternative name(s):
    DNA methyltransferase 01
    DNA methyltransferase 2
    DNA methyltransferase AthI
    Short name:
    DNA Metase AthI
    Short name:
    M.AthI
    DNA methyltransferase DDM2
    Protein DECREASED DNA METHYLATION 2
    Gene namesi
    Name:DMT1
    Synonyms:ATHIM, DDM2, DMT01, MET1, MET2
    Ordered Locus Names:At5g49160
    ORF Names:K21P3.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G49160.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Late-flowering phenotype caused by ectopic expression of FWA gene. Asymmetric zygote division and abnormal embryos. Displays genome hypomethylation, strong reduction of CpG methylation in centromeric repeats, reduction of heterochromatin and chromocenter size and gametes with fully demethylated and hemidemethylated DNA.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1101 – 11011G → S in met1-2/ddm2-2; reduced activity. 1 Publication
    Mutagenesisi1272 – 12721E → K in met1-5; reduced activity. 1 Publication
    Mutagenesisi1300 – 13001P → S in met1-1/ddm2-1; reduced activity. 1 Publication
    Mutagenesisi1465 – 14651G → E in met1-7; reduced activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15341534DNA (cytosine-5)-methyltransferase 1PRO_0000088039Add
    BLAST

    Proteomic databases

    PaxDbiP34881.
    PRIDEiP34881.

    Expressioni

    Tissue specificityi

    Expressed in flowers, embryos and endosperm.1 Publication

    Gene expression databases

    ArrayExpressiP34881.
    GenevestigatoriP34881.

    Interactioni

    Protein-protein interaction databases

    BioGridi20221. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP34881.
    SMRiP34881. Positions 710-1524.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini735 – 869135BAH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini909 – 1049141BAH 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1093 – 1527435SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 2 BAH domains.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0270.
    HOGENOMiHOG000083447.
    InParanoidiP34881.
    KOiK00558.
    OMAiDSYQFAG.
    PhylomeDBiP34881.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR001025. BAH_dom.
    IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR022702. Cytosine_MeTrfase1_RFD.
    IPR017198. DNA_C5-MeTrfase_1_euk.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF01426. BAH. 2 hits.
    PF00145. DNA_methylase. 2 hits.
    PF12047. DNMT1-RFD. 2 hits.
    [Graphical view]
    PIRSFiPIRSF037404. DNMT1. 1 hit.
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00439. BAH. 2 hits.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 3 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS51038. BAH. 2 hits.
    PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34881-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVENGAKAAK RKKRPLPEIQ EVEDVPRTRR PRRAAACTSF KEKSIRVCEK     50
    SATIEVKKQQ IVEEEFLALR LTALETDVED RPTRRLNDFV LFDSDGVPQP 100
    LEMLEIHDIF VSGAILPSDV CTDKEKEKGV RCTSFGRVEH WSISGYEDGS 150
    PVIWISTELA DYDCRKPAAS YRKVYDYFYE KARASVAVYK KLSKSSGGDP 200
    DIGLEELLAA VVRSMSSGSK YFSSGAAIID FVISQGDFIY NQLAGLDETA 250
    KKHESSYVEI PVLVALREKS SKIDKPLQRE RNPSNGVRIK EVSQVAESEA 300
    LTSDQLVDGT DDDRRYAILL QDEENRKSMQ QPRKNSSSGS ASNMFYIKIN 350
    EDEIANDYPL PSYYKTSEEE TDELILYDAS YEVQSEHLPH RMLHNWALYN 400
    SDLRFISLEL LPMKQCDDID VNIFGSGVVT DDNGSWISLN DPDSGSQSHD 450
    PDGMCIFLSQ IKEWMIEFGS DDIISISIRT DVAWYRLGKP SKLYAPWWKP 500
    VLKTARVGIS ILTFLRVESR VARLSFADVT KRLSGLQAND KAYISSDPLA 550
    VERYLVVHGQ IILQLFAVYP DDNVKRCPFV VGLASKLEDR HHTKWIIKKK 600
    KISLKELNLN PRAGMAPVAS KRKAMQATTT RLVNRIWGEF YSNYSPEDPL 650
    QATAAENGED EVEEEGGNGE EEVEEEGENG LTEDTVPEPV EVQKPHTPKK 700
    IRGSSGKREI KWDGESLGKT SAGEPLYQQA LVGGEMVAVG GAVTLEVDDP 750
    DEMPAIYFVE YMFESTDHCK MLHGRFLQRG SMTVLGNAAN ERELFLTNEC 800
    MTTQLKDIKG VASFEIRSRP WGHQYRKKNI TADKLDWARA LERKVKDLPT 850
    EYYCKSLYSP ERGGFFSLPL SDIGRSSGFC TSCKIREDEE KRSTIKLNVS 900
    KTGFFINGIE YSVEDFVYVN PDSIGGLKEG SKTSFKSGRN IGLRAYVVCQ 950
    LLEIVPKESR KADLGSFDVK VRRFYRPEDV SAEKAYASDI QELYFSQDTV 1000
    VLPPGALEGK CEVRKKSDMP LSREYPISDH IFFCDLFFDT SKGSLKQLPA 1050
    NMKPKFSTIK DDTLLRKKKG KGVESEIESE IVKPVEPPKE IRLATLDIFA 1100
    GCGGLSHGLK KAGVSDAKWA IEYEEPAGQA FKQNHPESTV FVDNCNVILR 1150
    AIMEKGGDQD DCVSTTEANE LAAKLTEEQK STLPLPGQVD FINGGPPCQG 1200
    FSGMNRFNQS SWSKVQCEMI LAFLSFADYF RPRYFLLENV RTFVSFNKGQ 1250
    TFQLTLASLL EMGYQVRFGI LEAGAYGVSQ SRKRAFIWAA APEEVLPEWP 1300
    EPMHVFGVPK LKISLSQGLH YAAVRSTALG APFRPITVRD TIGDLPSVEN 1350
    GDSRTNKEYK EVAVSWFQKE IRGNTIALTD HICKAMNELN LIRCKLIPTR 1400
    PGADWHDLPK RKVTLSDGRV EEMIPFCLPN TAERHNGWKG LYGRLDWQGN 1450
    FPTSVTDPQP MGKVGMCFHP EQHRILTVRE CARSQGFPDS YEFAGNINHK 1500
    HRQIGNAVPP PLAFALGRKL KEALHLKKSP QHQP 1534
    Length:1,534
    Mass (Da):172,431
    Last modified:February 1, 1994 - v1
    Checksum:i23FC944AA7074C5A
    GO

    Sequence cautioni

    The sequence AAX22756.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti306 – 3061L → M in AAX22756. 1 PublicationCurated
    Sequence conflicti837 – 8371W → R in AAX22756. 1 PublicationCurated
    Sequence conflicti901 – 9011K → R in AAX22756. 1 PublicationCurated
    Sequence conflicti932 – 9321K → N in BAF01962. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10692 mRNA. Translation: AAA32829.1.
    AB016872 Genomic DNA. Translation: BAB10334.1.
    CP002688 Genomic DNA. Translation: AED95777.1.
    AY699012 Genomic DNA. Translation: AAX22756.1. Sequence problems.
    AK229013 mRNA. Translation: BAF00900.1.
    AK230148 mRNA. Translation: BAF01962.1.
    PIRiS59604.
    RefSeqiNP_199727.1. NM_124293.3.
    UniGeneiAt.20294.

    Genome annotation databases

    EnsemblPlantsiAT5G49160.1; AT5G49160.1; AT5G49160.
    GeneIDi834975.
    KEGGiath:AT5G49160.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10692 mRNA. Translation: AAA32829.1 .
    AB016872 Genomic DNA. Translation: BAB10334.1 .
    CP002688 Genomic DNA. Translation: AED95777.1 .
    AY699012 Genomic DNA. Translation: AAX22756.1 . Sequence problems.
    AK229013 mRNA. Translation: BAF00900.1 .
    AK230148 mRNA. Translation: BAF01962.1 .
    PIRi S59604.
    RefSeqi NP_199727.1. NM_124293.3.
    UniGenei At.20294.

    3D structure databases

    ProteinModelPortali P34881.
    SMRi P34881. Positions 710-1524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20221. 3 interactions.

    Protein family/group databases

    REBASEi 11752. M.AthMET1.

    Proteomic databases

    PaxDbi P34881.
    PRIDEi P34881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G49160.1 ; AT5G49160.1 ; AT5G49160 .
    GeneIDi 834975.
    KEGGi ath:AT5G49160.

    Organism-specific databases

    TAIRi AT5G49160.

    Phylogenomic databases

    eggNOGi COG0270.
    HOGENOMi HOG000083447.
    InParanoidi P34881.
    KOi K00558.
    OMAi DSYQFAG.
    PhylomeDBi P34881.

    Enzyme and pathway databases

    BioCyci ARA:AT5G49160-MONOMER.

    Miscellaneous databases

    PROi P34881.

    Gene expression databases

    ArrayExpressi P34881.
    Genevestigatori P34881.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR001025. BAH_dom.
    IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR022702. Cytosine_MeTrfase1_RFD.
    IPR017198. DNA_C5-MeTrfase_1_euk.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF01426. BAH. 2 hits.
    PF00145. DNA_methylase. 2 hits.
    PF12047. DNMT1-RFD. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF037404. DNMT1. 1 hit.
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00439. BAH. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 3 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS51038. BAH. 2 hits.
    PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and identification by sequence homology of a putative cytosine methyltransferase from Arabidopsis thaliana."
      Finnegan E.J., Dennis E.S.
      Nucleic Acids Res. 21:2383-2388(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
      Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Identification of suppressors of transgene silencing in Arabidopsis."
      Chang S., Pikaard C.S.
      Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1517.
      Strain: cv. C24.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 932-1534 AND 1371-1534.
      Strain: cv. Columbia.
    6. "DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis."
      Soppe W.J.J., Jasencakova Z., Houben A., Kakutani T., Meister A., Huang M.S., Jacobsen S.E., Schubert I., Fransz P.F.
      EMBO J. 21:6549-6559(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase."
      Xiao W., Gehring M., Choi Y., Margossian L., Pu H., Harada J.J., Goldberg R.B., Pennell R.I., Fischer R.L.
      Dev. Cell 5:891-901(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLU-1272 AND GLY-1465.
    8. "Arabidopsis MET1 cytosine methyltransferase mutants."
      Kankel M.W., Ramsey D.E., Stokes T.L., Flowers S.K., Haag J.R., Jeddeloh J.A., Riddle N.C., Verbsky M.L., Richards E.J.
      Genetics 163:1109-1122(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLY-1101 AND PRO-1300.
    9. "Maintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis."
      Saze H., Mittelsten Scheid O., Paszkowski J.
      Nat. Genet. 34:65-69(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Regulation of seed size by hypomethylation of maternal and paternal genomes."
      Xiao W., Brown R.C., Lemmon B.E., Harada J.J., Goldberg R.B., Fischer R.L.
      Plant Physiol. 142:1160-1168(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    11. "DNA methylation is critical for Arabidopsis embryogenesis and seed viability."
      Xiao W., Custard K.D., Brown R.C., Lemmon B.E., Harada J.J., Goldberg R.B., Fischer R.L.
      Plant Cell 18:805-814(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    12. "Temporal and spatial downregulation of Arabidopsis MET1 activity results in global DNA hypomethylation and developmental defects."
      Kim M., Ohr H., Lee J.W., Hyun Y., Fischer R.L., Choi Y.
      Mol. Cells 26:611-615(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDNMT1_ARATH
    AccessioniPrimary (citable) accession number: P34881
    Secondary accession number(s): Q0WLP5, Q0WPP9, Q5C994
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3