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P34881 (DNMT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA (cytosine-5)-methyltransferase 1

EC=2.1.1.37
Alternative name(s):
DNA methyltransferase 01
DNA methyltransferase 2
DNA methyltransferase AthI
Short name=DNA Metase AthI
Short name=M.AthI
DNA methyltransferase DDM2
Protein DECREASED DNA METHYLATION 2
Gene names
Name:DMT1
Synonyms:ATHIM, DDM2, DMT01, MET1, MET2
Ordered Locus Names:At5g49160
ORF Names:K21P3.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. Required for proper patterns of CG DNA methylation in dividing cells. Required for MEA promoter methylation in seeds. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in flowers, embryos and endosperm. Ref.10

Disruption phenotype

Late-flowering phenotype caused by ectopic expression of FWA gene. Asymmetric zygote division and abnormal embryos. Displays genome hypomethylation, strong reduction of CpG methylation in centromeric repeats, reduction of heterochromatin and chromocenter size and gametes with fully demethylated and hemidemethylated DNA. Ref.11

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 2 BAH domains.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence caution

The sequence AAX22756.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15341534DNA (cytosine-5)-methyltransferase 1
PRO_0000088039

Regions

Domain735 – 869135BAH 1
Domain909 – 1049141BAH 2
Domain1093 – 1527435SAM-dependent MTase C5-type

Sites

Active site11981 By similarity

Experimental info

Mutagenesis11011G → S in met1-2/ddm2-2; reduced activity. Ref.8
Mutagenesis12721E → K in met1-5; reduced activity. Ref.7
Mutagenesis13001P → S in met1-1/ddm2-1; reduced activity. Ref.8
Mutagenesis14651G → E in met1-7; reduced activity. Ref.7
Sequence conflict3061L → M in AAX22756. Ref.4
Sequence conflict8371W → R in AAX22756. Ref.4
Sequence conflict9011K → R in AAX22756. Ref.4
Sequence conflict9321K → N in BAF01962. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P34881 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 23FC944AA7074C5A

FASTA1,534172,431
        10         20         30         40         50         60 
MVENGAKAAK RKKRPLPEIQ EVEDVPRTRR PRRAAACTSF KEKSIRVCEK SATIEVKKQQ 

        70         80         90        100        110        120 
IVEEEFLALR LTALETDVED RPTRRLNDFV LFDSDGVPQP LEMLEIHDIF VSGAILPSDV 

       130        140        150        160        170        180 
CTDKEKEKGV RCTSFGRVEH WSISGYEDGS PVIWISTELA DYDCRKPAAS YRKVYDYFYE 

       190        200        210        220        230        240 
KARASVAVYK KLSKSSGGDP DIGLEELLAA VVRSMSSGSK YFSSGAAIID FVISQGDFIY 

       250        260        270        280        290        300 
NQLAGLDETA KKHESSYVEI PVLVALREKS SKIDKPLQRE RNPSNGVRIK EVSQVAESEA 

       310        320        330        340        350        360 
LTSDQLVDGT DDDRRYAILL QDEENRKSMQ QPRKNSSSGS ASNMFYIKIN EDEIANDYPL 

       370        380        390        400        410        420 
PSYYKTSEEE TDELILYDAS YEVQSEHLPH RMLHNWALYN SDLRFISLEL LPMKQCDDID 

       430        440        450        460        470        480 
VNIFGSGVVT DDNGSWISLN DPDSGSQSHD PDGMCIFLSQ IKEWMIEFGS DDIISISIRT 

       490        500        510        520        530        540 
DVAWYRLGKP SKLYAPWWKP VLKTARVGIS ILTFLRVESR VARLSFADVT KRLSGLQAND 

       550        560        570        580        590        600 
KAYISSDPLA VERYLVVHGQ IILQLFAVYP DDNVKRCPFV VGLASKLEDR HHTKWIIKKK 

       610        620        630        640        650        660 
KISLKELNLN PRAGMAPVAS KRKAMQATTT RLVNRIWGEF YSNYSPEDPL QATAAENGED 

       670        680        690        700        710        720 
EVEEEGGNGE EEVEEEGENG LTEDTVPEPV EVQKPHTPKK IRGSSGKREI KWDGESLGKT 

       730        740        750        760        770        780 
SAGEPLYQQA LVGGEMVAVG GAVTLEVDDP DEMPAIYFVE YMFESTDHCK MLHGRFLQRG 

       790        800        810        820        830        840 
SMTVLGNAAN ERELFLTNEC MTTQLKDIKG VASFEIRSRP WGHQYRKKNI TADKLDWARA 

       850        860        870        880        890        900 
LERKVKDLPT EYYCKSLYSP ERGGFFSLPL SDIGRSSGFC TSCKIREDEE KRSTIKLNVS 

       910        920        930        940        950        960 
KTGFFINGIE YSVEDFVYVN PDSIGGLKEG SKTSFKSGRN IGLRAYVVCQ LLEIVPKESR 

       970        980        990       1000       1010       1020 
KADLGSFDVK VRRFYRPEDV SAEKAYASDI QELYFSQDTV VLPPGALEGK CEVRKKSDMP 

      1030       1040       1050       1060       1070       1080 
LSREYPISDH IFFCDLFFDT SKGSLKQLPA NMKPKFSTIK DDTLLRKKKG KGVESEIESE 

      1090       1100       1110       1120       1130       1140 
IVKPVEPPKE IRLATLDIFA GCGGLSHGLK KAGVSDAKWA IEYEEPAGQA FKQNHPESTV 

      1150       1160       1170       1180       1190       1200 
FVDNCNVILR AIMEKGGDQD DCVSTTEANE LAAKLTEEQK STLPLPGQVD FINGGPPCQG 

      1210       1220       1230       1240       1250       1260 
FSGMNRFNQS SWSKVQCEMI LAFLSFADYF RPRYFLLENV RTFVSFNKGQ TFQLTLASLL 

      1270       1280       1290       1300       1310       1320 
EMGYQVRFGI LEAGAYGVSQ SRKRAFIWAA APEEVLPEWP EPMHVFGVPK LKISLSQGLH 

      1330       1340       1350       1360       1370       1380 
YAAVRSTALG APFRPITVRD TIGDLPSVEN GDSRTNKEYK EVAVSWFQKE IRGNTIALTD 

      1390       1400       1410       1420       1430       1440 
HICKAMNELN LIRCKLIPTR PGADWHDLPK RKVTLSDGRV EEMIPFCLPN TAERHNGWKG 

      1450       1460       1470       1480       1490       1500 
LYGRLDWQGN FPTSVTDPQP MGKVGMCFHP EQHRILTVRE CARSQGFPDS YEFAGNINHK 

      1510       1520       1530 
HRQIGNAVPP PLAFALGRKL KEALHLKKSP QHQP 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and identification by sequence homology of a putative cytosine methyltransferase from Arabidopsis thaliana."
Finnegan E.J., Dennis E.S.
Nucleic Acids Res. 21:2383-2388(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Identification of suppressors of transgene silencing in Arabidopsis."
Chang S., Pikaard C.S.
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1517.
Strain: cv. C24.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 932-1534 AND 1371-1534.
Strain: cv. Columbia.
[6]"DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis."
Soppe W.J.J., Jasencakova Z., Houben A., Kakutani T., Meister A., Huang M.S., Jacobsen S.E., Schubert I., Fransz P.F.
EMBO J. 21:6549-6559(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase."
Xiao W., Gehring M., Choi Y., Margossian L., Pu H., Harada J.J., Goldberg R.B., Pennell R.I., Fischer R.L.
Dev. Cell 5:891-901(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLU-1272 AND GLY-1465.
[8]"Arabidopsis MET1 cytosine methyltransferase mutants."
Kankel M.W., Ramsey D.E., Stokes T.L., Flowers S.K., Haag J.R., Jeddeloh J.A., Riddle N.C., Verbsky M.L., Richards E.J.
Genetics 163:1109-1122(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-1101 AND PRO-1300.
[9]"Maintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis."
Saze H., Mittelsten Scheid O., Paszkowski J.
Nat. Genet. 34:65-69(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Regulation of seed size by hypomethylation of maternal and paternal genomes."
Xiao W., Brown R.C., Lemmon B.E., Harada J.J., Goldberg R.B., Fischer R.L.
Plant Physiol. 142:1160-1168(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[11]"DNA methylation is critical for Arabidopsis embryogenesis and seed viability."
Xiao W., Custard K.D., Brown R.C., Lemmon B.E., Harada J.J., Goldberg R.B., Fischer R.L.
Plant Cell 18:805-814(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[12]"Temporal and spatial downregulation of Arabidopsis MET1 activity results in global DNA hypomethylation and developmental defects."
Kim M., Ohr H., Lee J.W., Hyun Y., Fischer R.L., Choi Y.
Mol. Cells 26:611-615(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10692 mRNA. Translation: AAA32829.1.
AB016872 Genomic DNA. Translation: BAB10334.1.
CP002688 Genomic DNA. Translation: AED95777.1.
AY699012 Genomic DNA. Translation: AAX22756.1. Sequence problems.
AK229013 mRNA. Translation: BAF00900.1.
AK230148 mRNA. Translation: BAF01962.1.
PIRS59604.
RefSeqNP_199727.1. NM_124293.3.
UniGeneAt.20294.

3D structure databases

ProteinModelPortalP34881.
SMRP34881. Positions 710-1524.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20221. 3 interactions.

Protein family/group databases

REBASE11752. M.AthMET1.

Proteomic databases

PaxDbP34881.
PRIDEP34881.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G49160.1; AT5G49160.1; AT5G49160.
GeneID834975.
KEGGath:AT5G49160.

Organism-specific databases

TAIRAT5G49160.

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000083447.
InParanoidP34881.
KOK00558.
OMADSYQFAG.
PhylomeDBP34881.

Enzyme and pathway databases

BioCycARA:AT5G49160-MONOMER.

Gene expression databases

ArrayExpressP34881.
GenevestigatorP34881.

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR022702. Cytosine_MeTrfase1_RFD.
IPR017198. DNA_C5-MeTrfase_1_euk.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF01426. BAH. 2 hits.
PF00145. DNA_methylase. 2 hits.
PF12047. DNMT1-RFD. 2 hits.
[Graphical view]
PIRSFPIRSF037404. DNMT1. 1 hit.
PRINTSPR00105. C5METTRFRASE.
SMARTSM00439. BAH. 2 hits.
[Graphical view]
SUPFAMSSF53335. SSF53335. 3 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS51038. BAH. 2 hits.
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP34881.

Entry information

Entry nameDNMT1_ARATH
AccessionPrimary (citable) accession number: P34881
Secondary accession number(s): Q0WLP5, Q0WPP9, Q5C994
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names