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P34881

- DNMT1_ARATH

UniProt

P34881 - DNMT1_ARATH

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Protein

DNA (cytosine-5)-methyltransferase 1

Gene

DMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. Required for proper patterns of CG DNA methylation in dividing cells. Required for MEA promoter methylation in seeds.7 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1198 – 11981PROSITE-ProRule annotation

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  3. DNA binding Source: UniProtKB-KW
  4. methyltransferase activity Source: TAIR

GO - Biological processi

  1. chromatin modification Source: UniProtKB-KW
  2. DNA mediated transformation Source: TAIR
  3. DNA methylation on cytosine within a CG sequence Source: TAIR
  4. maintenance of DNA methylation Source: TAIR
  5. negative regulation of flower development Source: TAIR
  6. regulation of gene expression by genetic imprinting Source: TAIR
  7. zygote asymmetric cytokinesis in embryo sac Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G49160-MONOMER.

Protein family/group databases

REBASEi11752. M.AthMET1.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 1 (EC:2.1.1.37)
Alternative name(s):
DNA methyltransferase 01
DNA methyltransferase 2
DNA methyltransferase AthI
Short name:
DNA Metase AthI
Short name:
M.AthI
DNA methyltransferase DDM2
Protein DECREASED DNA METHYLATION 2
Gene namesi
Name:DMT1
Synonyms:ATHIM, DDM2, DMT01, MET1, MET2
Ordered Locus Names:At5g49160
ORF Names:K21P3.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G49160.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Late-flowering phenotype caused by ectopic expression of FWA gene. Asymmetric zygote division and abnormal embryos. Displays genome hypomethylation, strong reduction of CpG methylation in centromeric repeats, reduction of heterochromatin and chromocenter size and gametes with fully demethylated and hemidemethylated DNA.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1101 – 11011G → S in met1-2/ddm2-2; reduced activity. 1 Publication
Mutagenesisi1272 – 12721E → K in met1-5; reduced activity. 1 Publication
Mutagenesisi1300 – 13001P → S in met1-1/ddm2-1; reduced activity. 1 Publication
Mutagenesisi1465 – 14651G → E in met1-7; reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15341534DNA (cytosine-5)-methyltransferase 1PRO_0000088039Add
BLAST

Proteomic databases

PaxDbiP34881.
PRIDEiP34881.

Expressioni

Tissue specificityi

Expressed in flowers, embryos and endosperm.1 Publication

Gene expression databases

ExpressionAtlasiP34881. baseline and differential.
GenevestigatoriP34881.

Interactioni

Protein-protein interaction databases

BioGridi20221. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP34881.
SMRiP34881. Positions 710-1524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini735 – 869135BAH 1PROSITE-ProRule annotationAdd
BLAST
Domaini909 – 1049141BAH 2PROSITE-ProRule annotationAdd
BLAST
Domaini1093 – 1527435SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 2 BAH domains.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000083447.
InParanoidiP34881.
KOiK00558.
OMAiDSYQFAG.
PhylomeDBiP34881.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR022702. Cytosine_MeTrfase1_RFD.
IPR017198. DNA_C5-MeTrfase_1_euk.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF01426. BAH. 2 hits.
PF00145. DNA_methylase. 2 hits.
PF12047. DNMT1-RFD. 2 hits.
[Graphical view]
PIRSFiPIRSF037404. DNMT1. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00439. BAH. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51038. BAH. 2 hits.
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34881-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVENGAKAAK RKKRPLPEIQ EVEDVPRTRR PRRAAACTSF KEKSIRVCEK
60 70 80 90 100
SATIEVKKQQ IVEEEFLALR LTALETDVED RPTRRLNDFV LFDSDGVPQP
110 120 130 140 150
LEMLEIHDIF VSGAILPSDV CTDKEKEKGV RCTSFGRVEH WSISGYEDGS
160 170 180 190 200
PVIWISTELA DYDCRKPAAS YRKVYDYFYE KARASVAVYK KLSKSSGGDP
210 220 230 240 250
DIGLEELLAA VVRSMSSGSK YFSSGAAIID FVISQGDFIY NQLAGLDETA
260 270 280 290 300
KKHESSYVEI PVLVALREKS SKIDKPLQRE RNPSNGVRIK EVSQVAESEA
310 320 330 340 350
LTSDQLVDGT DDDRRYAILL QDEENRKSMQ QPRKNSSSGS ASNMFYIKIN
360 370 380 390 400
EDEIANDYPL PSYYKTSEEE TDELILYDAS YEVQSEHLPH RMLHNWALYN
410 420 430 440 450
SDLRFISLEL LPMKQCDDID VNIFGSGVVT DDNGSWISLN DPDSGSQSHD
460 470 480 490 500
PDGMCIFLSQ IKEWMIEFGS DDIISISIRT DVAWYRLGKP SKLYAPWWKP
510 520 530 540 550
VLKTARVGIS ILTFLRVESR VARLSFADVT KRLSGLQAND KAYISSDPLA
560 570 580 590 600
VERYLVVHGQ IILQLFAVYP DDNVKRCPFV VGLASKLEDR HHTKWIIKKK
610 620 630 640 650
KISLKELNLN PRAGMAPVAS KRKAMQATTT RLVNRIWGEF YSNYSPEDPL
660 670 680 690 700
QATAAENGED EVEEEGGNGE EEVEEEGENG LTEDTVPEPV EVQKPHTPKK
710 720 730 740 750
IRGSSGKREI KWDGESLGKT SAGEPLYQQA LVGGEMVAVG GAVTLEVDDP
760 770 780 790 800
DEMPAIYFVE YMFESTDHCK MLHGRFLQRG SMTVLGNAAN ERELFLTNEC
810 820 830 840 850
MTTQLKDIKG VASFEIRSRP WGHQYRKKNI TADKLDWARA LERKVKDLPT
860 870 880 890 900
EYYCKSLYSP ERGGFFSLPL SDIGRSSGFC TSCKIREDEE KRSTIKLNVS
910 920 930 940 950
KTGFFINGIE YSVEDFVYVN PDSIGGLKEG SKTSFKSGRN IGLRAYVVCQ
960 970 980 990 1000
LLEIVPKESR KADLGSFDVK VRRFYRPEDV SAEKAYASDI QELYFSQDTV
1010 1020 1030 1040 1050
VLPPGALEGK CEVRKKSDMP LSREYPISDH IFFCDLFFDT SKGSLKQLPA
1060 1070 1080 1090 1100
NMKPKFSTIK DDTLLRKKKG KGVESEIESE IVKPVEPPKE IRLATLDIFA
1110 1120 1130 1140 1150
GCGGLSHGLK KAGVSDAKWA IEYEEPAGQA FKQNHPESTV FVDNCNVILR
1160 1170 1180 1190 1200
AIMEKGGDQD DCVSTTEANE LAAKLTEEQK STLPLPGQVD FINGGPPCQG
1210 1220 1230 1240 1250
FSGMNRFNQS SWSKVQCEMI LAFLSFADYF RPRYFLLENV RTFVSFNKGQ
1260 1270 1280 1290 1300
TFQLTLASLL EMGYQVRFGI LEAGAYGVSQ SRKRAFIWAA APEEVLPEWP
1310 1320 1330 1340 1350
EPMHVFGVPK LKISLSQGLH YAAVRSTALG APFRPITVRD TIGDLPSVEN
1360 1370 1380 1390 1400
GDSRTNKEYK EVAVSWFQKE IRGNTIALTD HICKAMNELN LIRCKLIPTR
1410 1420 1430 1440 1450
PGADWHDLPK RKVTLSDGRV EEMIPFCLPN TAERHNGWKG LYGRLDWQGN
1460 1470 1480 1490 1500
FPTSVTDPQP MGKVGMCFHP EQHRILTVRE CARSQGFPDS YEFAGNINHK
1510 1520 1530
HRQIGNAVPP PLAFALGRKL KEALHLKKSP QHQP
Length:1,534
Mass (Da):172,431
Last modified:February 1, 1994 - v1
Checksum:i23FC944AA7074C5A
GO

Sequence cautioni

The sequence AAX22756.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 3061L → M in AAX22756. 1 PublicationCurated
Sequence conflicti837 – 8371W → R in AAX22756. 1 PublicationCurated
Sequence conflicti901 – 9011K → R in AAX22756. 1 PublicationCurated
Sequence conflicti932 – 9321K → N in BAF01962. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10692 mRNA. Translation: AAA32829.1.
AB016872 Genomic DNA. Translation: BAB10334.1.
CP002688 Genomic DNA. Translation: AED95777.1.
AY699012 Genomic DNA. Translation: AAX22756.1. Sequence problems.
AK229013 mRNA. Translation: BAF00900.1.
AK230148 mRNA. Translation: BAF01962.1.
PIRiS59604.
RefSeqiNP_199727.1. NM_124293.3.
UniGeneiAt.20294.

Genome annotation databases

EnsemblPlantsiAT5G49160.1; AT5G49160.1; AT5G49160.
GeneIDi834975.
KEGGiath:AT5G49160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10692 mRNA. Translation: AAA32829.1 .
AB016872 Genomic DNA. Translation: BAB10334.1 .
CP002688 Genomic DNA. Translation: AED95777.1 .
AY699012 Genomic DNA. Translation: AAX22756.1 . Sequence problems.
AK229013 mRNA. Translation: BAF00900.1 .
AK230148 mRNA. Translation: BAF01962.1 .
PIRi S59604.
RefSeqi NP_199727.1. NM_124293.3.
UniGenei At.20294.

3D structure databases

ProteinModelPortali P34881.
SMRi P34881. Positions 710-1524.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20221. 3 interactions.

Protein family/group databases

REBASEi 11752. M.AthMET1.

Proteomic databases

PaxDbi P34881.
PRIDEi P34881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G49160.1 ; AT5G49160.1 ; AT5G49160 .
GeneIDi 834975.
KEGGi ath:AT5G49160.

Organism-specific databases

TAIRi AT5G49160.

Phylogenomic databases

eggNOGi COG0270.
HOGENOMi HOG000083447.
InParanoidi P34881.
KOi K00558.
OMAi DSYQFAG.
PhylomeDBi P34881.

Enzyme and pathway databases

BioCyci ARA:AT5G49160-MONOMER.

Miscellaneous databases

PROi P34881.

Gene expression databases

ExpressionAtlasi P34881. baseline and differential.
Genevestigatori P34881.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR001025. BAH_dom.
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR022702. Cytosine_MeTrfase1_RFD.
IPR017198. DNA_C5-MeTrfase_1_euk.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF01426. BAH. 2 hits.
PF00145. DNA_methylase. 2 hits.
PF12047. DNMT1-RFD. 2 hits.
[Graphical view ]
PIRSFi PIRSF037404. DNMT1. 1 hit.
PRINTSi PR00105. C5METTRFRASE.
SMARTi SM00439. BAH. 2 hits.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS51038. BAH. 2 hits.
PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and identification by sequence homology of a putative cytosine methyltransferase from Arabidopsis thaliana."
    Finnegan E.J., Dennis E.S.
    Nucleic Acids Res. 21:2383-2388(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Identification of suppressors of transgene silencing in Arabidopsis."
    Chang S., Pikaard C.S.
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1517.
    Strain: cv. C24.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 932-1534 AND 1371-1534.
    Strain: cv. Columbia.
  6. "DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis."
    Soppe W.J.J., Jasencakova Z., Houben A., Kakutani T., Meister A., Huang M.S., Jacobsen S.E., Schubert I., Fransz P.F.
    EMBO J. 21:6549-6559(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase."
    Xiao W., Gehring M., Choi Y., Margossian L., Pu H., Harada J.J., Goldberg R.B., Pennell R.I., Fischer R.L.
    Dev. Cell 5:891-901(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-1272 AND GLY-1465.
  8. "Arabidopsis MET1 cytosine methyltransferase mutants."
    Kankel M.W., Ramsey D.E., Stokes T.L., Flowers S.K., Haag J.R., Jeddeloh J.A., Riddle N.C., Verbsky M.L., Richards E.J.
    Genetics 163:1109-1122(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-1101 AND PRO-1300.
  9. "Maintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis."
    Saze H., Mittelsten Scheid O., Paszkowski J.
    Nat. Genet. 34:65-69(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Regulation of seed size by hypomethylation of maternal and paternal genomes."
    Xiao W., Brown R.C., Lemmon B.E., Harada J.J., Goldberg R.B., Fischer R.L.
    Plant Physiol. 142:1160-1168(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "DNA methylation is critical for Arabidopsis embryogenesis and seed viability."
    Xiao W., Custard K.D., Brown R.C., Lemmon B.E., Harada J.J., Goldberg R.B., Fischer R.L.
    Plant Cell 18:805-814(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  12. "Temporal and spatial downregulation of Arabidopsis MET1 activity results in global DNA hypomethylation and developmental defects."
    Kim M., Ohr H., Lee J.W., Hyun Y., Fischer R.L., Choi Y.
    Mol. Cells 26:611-615(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDNMT1_ARATH
AccessioniPrimary (citable) accession number: P34881
Secondary accession number(s): Q0WLP5, Q0WPP9, Q5C994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3