P34880 (T2S2_SHISO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme SsoII
Short name=R.SsoII
EC=3.1.21.4

Alternative name(s):
Endonuclease SsoII
Type II restriction enzyme SsoII

Gene names

Name:

ssoIIR

Encoded on

Plasmid P4

Organism

Shigella sonnei

Taxonomic identifier

624 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCNGG and cleaves before C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Type-2 restriction enzyme SsoII		PRO_0000077363

Sequences

	Sequence		Length	Mass (Da)
	P34880 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 61FDF54AB0C02D29	FASTA	305	35,939
	10 20 30 40 50 60 MQRLSPGEFK TLISKERKSH FITPFALVYK TFCDLGYDQK NSDYFLNNPS EYIIAMRKNC 70 80 90 100 110 120 WKEFEPFEKE FTTRMLSYLI DEERIKDMSP YDAIRDFTME YPTHIYDLAL SNTQSRRSRA 130 140 150 160 170 180 GKEFESILEL LMMGAGIPVD VQGAIGKSFF QKNQIGKLVD LVMPGVVQYT SNKRNTMLIS 190 200 210 220 230 240 AKTTLRERWQ EVPEEVNRTG IREMYLATLD DSFSEETINI LYEANVVVVT TIENKNFKYK 250 260 270 280 290 300 NNNRVLTFED MLQSAMELSR KWNNVSYTDS EKEEIQRSIL KQIEKYSDFP YVVNYYRNRL SALFD

References

[1]

"Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase." Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I. Gene 124:13-19(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 47.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86545 Genomic DNA. Translation: AAA98280.1.
PIR	JU0156.
3D structure databases
ProteinModelPortal	P34880.
SMR	P34880. Positions 3-305.
ModBase	Search...
Protein family/group databases
REBASE	1739. SsoII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011335. Restrct_endonuc-II-like. IPR015109. Restrct_endonuc_II_EcoRII_C. [Graphical view]
Pfam	PF09019. EcoRII-C. 1 hit. [Graphical view]
SUPFAM	SSF52980. Restrict_endonuc_II-like_core. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2S2_SHISO

Accession

Primary (citable) accession number: P34880

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program