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P34880 (T2S2_SHISO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme SsoII

Short name=R.SsoII
EC=3.1.21.4
Alternative name(s):
Endonuclease SsoII
Type II restriction enzyme SsoII
Gene names
Name:ssoIIR
Encoded onPlasmid P4
OrganismShigella sonnei
Taxonomic identifier624 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCNGG and cleaves before C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Type-2 restriction enzyme SsoII
PRO_0000077363

Sequences

Sequence LengthMass (Da)Tools
P34880 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 61FDF54AB0C02D29

FASTA30535,939
        10         20         30         40         50         60 
MQRLSPGEFK TLISKERKSH FITPFALVYK TFCDLGYDQK NSDYFLNNPS EYIIAMRKNC 

        70         80         90        100        110        120 
WKEFEPFEKE FTTRMLSYLI DEERIKDMSP YDAIRDFTME YPTHIYDLAL SNTQSRRSRA 

       130        140        150        160        170        180 
GKEFESILEL LMMGAGIPVD VQGAIGKSFF QKNQIGKLVD LVMPGVVQYT SNKRNTMLIS 

       190        200        210        220        230        240 
AKTTLRERWQ EVPEEVNRTG IREMYLATLD DSFSEETINI LYEANVVVVT TIENKNFKYK 

       250        260        270        280        290        300 
NNNRVLTFED MLQSAMELSR KWNNVSYTDS EKEEIQRSIL KQIEKYSDFP YVVNYYRNRL 


SALFD 

« Hide

References

[1]"Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase."
Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.
Gene 124:13-19(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86545 Genomic DNA. Translation: AAA98280.1.
PIRJU0156.

3D structure databases

ProteinModelPortalP34880.
SMRP34880. Positions 3-305.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1739. SsoII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011335. Restrct_endonuc-II-like.
IPR015109. Restrct_endonuc_II_EcoRII_C.
[Graphical view]
PfamPF09019. EcoRII-C. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2S2_SHISO
AccessionPrimary (citable) accession number: P34880
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries