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P34880

- T2S2_SHISO

UniProt

P34880 - T2S2_SHISO

Protein

Type-2 restriction enzyme SsoII

Gene

ssoIIR

Organism
Shigella sonnei
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 55 (16 Apr 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CCNGG and cleaves before C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1739. SsoII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme SsoII (EC:3.1.21.4)
    Short name:
    R.SsoII
    Alternative name(s):
    Endonuclease SsoII
    Type II restriction enzyme SsoII
    Gene namesi
    Name:ssoIIR
    Encoded oniPlasmid P40 Publication
    OrganismiShigella sonnei
    Taxonomic identifieri624 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Type-2 restriction enzyme SsoIIPRO_0000077363Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP34880.
    SMRiP34880. Positions 3-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR015109. Restrct_endonuc_II_EcoRII_C.
    [Graphical view]
    PfamiPF09019. EcoRII-C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34880-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQRLSPGEFK TLISKERKSH FITPFALVYK TFCDLGYDQK NSDYFLNNPS    50
    EYIIAMRKNC WKEFEPFEKE FTTRMLSYLI DEERIKDMSP YDAIRDFTME 100
    YPTHIYDLAL SNTQSRRSRA GKEFESILEL LMMGAGIPVD VQGAIGKSFF 150
    QKNQIGKLVD LVMPGVVQYT SNKRNTMLIS AKTTLRERWQ EVPEEVNRTG 200
    IREMYLATLD DSFSEETINI LYEANVVVVT TIENKNFKYK NNNRVLTFED 250
    MLQSAMELSR KWNNVSYTDS EKEEIQRSIL KQIEKYSDFP YVVNYYRNRL 300
    SALFD 305
    Length:305
    Mass (Da):35,939
    Last modified:February 1, 1994 - v1
    Checksum:i61FDF54AB0C02D29
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86545 Genomic DNA. Translation: AAA98280.1.
    PIRiJU0156.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86545 Genomic DNA. Translation: AAA98280.1 .
    PIRi JU0156.

    3D structure databases

    ProteinModelPortali P34880.
    SMRi P34880. Positions 3-305.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1739. SsoII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR015109. Restrct_endonuc_II_EcoRII_C.
    [Graphical view ]
    Pfami PF09019. EcoRII-C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase."
      Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.
      Gene 124:13-19(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 47.

    Entry informationi

    Entry nameiT2S2_SHISO
    AccessioniPrimary (citable) accession number: P34880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: April 16, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3