Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P34880 (T2S2_SHISO)

Last modified November 3, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme SsoII
      Short name=R.SsoII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme SsoII
    Endonuclease SsoII
Gene names
Name: ssoIIR
Encoded onPlasmid P4
OrganismShigella sonnei
Taxonomic identifier624 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existencePredicted.

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCNGG and cleaves before C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Type-2 restriction enzyme SsoII
PRO_0000077363

Sequences

Sequence LengthMass (Da)Tools
P34880-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 61FDF54AB0C02D29

FASTA30535,939
        10         20         30         40         50         60 
MQRLSPGEFK TLISKERKSH FITPFALVYK TFCDLGYDQK NSDYFLNNPS EYIIAMRKNC 

        70         80         90        100        110        120 
WKEFEPFEKE FTTRMLSYLI DEERIKDMSP YDAIRDFTME YPTHIYDLAL SNTQSRRSRA 

       130        140        150        160        170        180 
GKEFESILEL LMMGAGIPVD VQGAIGKSFF QKNQIGKLVD LVMPGVVQYT SNKRNTMLIS 

       190        200        210        220        230        240 
AKTTLRERWQ EVPEEVNRTG IREMYLATLD DSFSEETINI LYEANVVVVT TIENKNFKYK 

       250        260        270        280        290        300 
NNNRVLTFED MLQSAMELSR KWNNVSYTDS EKEEIQRSIL KQIEKYSDFP YVVNYYRNRL 


SALFD 

« Hide

References

[1]"Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase."
Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.
Gene 124:13-19(1993) [PubMed: 7916706] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 47.

Cross-references

Sequence databases

M86545 Genomic DNA. Translation: AAA98280.1.
PIRJU0156.

3D structure databases

SMRP34880. Positions 3-305.
ModBaseSearch...

Protein family/group databases

REBASE1739. SsoII.

Enzyme and pathway databases

BRENDA3.1.21.4. 74546.

Family and domain databases

InterProIPR015109. Restrict_endonuc_II_EcoRII_C.
[Graphical view]
PfamPF09019. EcoRII-C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S2_SHISO
AccessionPrimary (citable) accession number: P34880
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents