Reviewed, UniProtKB/Swiss-Prot P34880 (T2S2_SHISO)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme SsoII
      Short name=R.SsoII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme SsoII
    Endonuclease SsoII

Gene names

Name:

ssoIIR

Encoded on

Plasmid P4

Organism

Shigella sonnei

Taxonomic identifier

624 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCNGG and cleaves before C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Type-2 restriction enzyme SsoII		PRO_0000077363

Sequences

	Sequence		Length	Mass (Da)
	P34880-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 61FDF54AB0C02D29	FASTA	305	35,939
	10 20 30 40 50 60 MQRLSPGEFK TLISKERKSH FITPFALVYK TFCDLGYDQK NSDYFLNNPS EYIIAMRKNC 70 80 90 100 110 120 WKEFEPFEKE FTTRMLSYLI DEERIKDMSP YDAIRDFTME YPTHIYDLAL SNTQSRRSRA 130 140 150 160 170 180 GKEFESILEL LMMGAGIPVD VQGAIGKSFF QKNQIGKLVD LVMPGVVQYT SNKRNTMLIS 190 200 210 220 230 240 AKTTLRERWQ EVPEEVNRTG IREMYLATLD DSFSEETINI LYEANVVVVT TIENKNFKYK 250 260 270 280 290 300 NNNRVLTFED MLQSAMELSR KWNNVSYTDS EKEEIQRSIL KQIEKYSDFP YVVNYYRNRL SALFD

References

[1]

"Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase." Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I. Gene 124:13-19(1993) [PubMed: 7916706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 47.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86545 Genomic DNA. Translation: AAA98280.1.
PIR	JU0156.
3D structure databases
SMR	P34880. Positions 3-305.
ModBase	Search...
Protein family/group databases
REBASE	1739. SsoII.
Enzyme and pathway databases
BRENDA	3.1.21.4. 74546.
Family and domain databases
InterPro	IPR015109. Restrict_endonuc_II_EcoRII_C. [Graphical view]
Pfam	PF09019. EcoRII-C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S2_SHISO

Accession

Primary (citable) accession number: P34880

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)