Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P34879

- MTS2_SHISO

UniProt

P34879 - MTS2_SHISO

Protein

Modification methylase SsoII

Gene

ssoIIM

Organism
Shigella sonnei
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SsoII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei142 – 1421PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3509. M.SsoII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase SsoII (EC:2.1.1.37)
    Short name:
    M.SsoII
    Alternative name(s):
    Cytosine-specific methyltransferase SsoII
    Gene namesi
    Name:ssoIIM
    Encoded oniPlasmid P40 Publication
    OrganismiShigella sonnei
    Taxonomic identifieri624 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Modification methylase SsoIIPRO_0000087906Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP34879.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 6658HTH cro/C1-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini72 – 379308SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    PF01381. HTH_3. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34879-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDNIAATIK EKRERLHMTQ KEFADALGLS KYGDRTIRRW ERGETKPTGA    50
    ELKAVIDFPD TPPYPNNENG RYRMIDLFAG IGGTRLGFHQ TNAVNVVFSS 100
    EWDKFAQKTY HANYGDFPDG DITKIDEKDI PDHEILVGGF PCVAFSQAGL 150
    KKGFNDTRGT LFFDIARIIK EKKPHAFLLE NVKNLLGHDK GRTFSIIKNT 200
    LEELNYTVYY NIFAAKDFGV PQNRERIYIV GFNKEKVRNH EHFTFPTPLK 250
    TKTRVGDILE KSVDNKYTLS DALWNGHQRR KLVNAAAGKG FGYGLFNENS 300
    PYTNTISARY YKDGSEILIE QKGSNPRKIT PREASRLQGF PSDFIIPVSD 350
    TQAYKQFGNS VAVPVINAIA EKIISTLDS 379
    Length:379
    Mass (Da):42,890
    Last modified:February 1, 1994 - v1
    Checksum:i97440A79FC6AB275
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86545 Genomic DNA. Translation: AAA98279.1.
    PIRiJT0744.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86545 Genomic DNA. Translation: AAA98279.1 .
    PIRi JT0744.

    3D structure databases

    ProteinModelPortali P34879.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3509. M.SsoII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    PF01381. HTH_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase."
      Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.
      Gene 124:13-19(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 47.

    Entry informationi

    Entry nameiMTS2_SHISO
    AccessioniPrimary (citable) accession number: P34879
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3