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P34879 (MTS2_SHISO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase SsoII

Short name=M.SsoII
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase SsoII
Gene names
Name:ssoIIM
Encoded onPlasmid P4
OrganismShigella sonnei
Taxonomic identifier624 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SsoII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 HTH cro/C1-type DNA-binding domain.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Modification methylase SsoII
PRO_0000087906

Regions

Domain9 – 6658HTH cro/C1-type
Domain72 – 379308SAM-dependent MTase C5-type

Sites

Active site1421 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34879 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 97440A79FC6AB275

FASTA37942,890
        10         20         30         40         50         60 
MTDNIAATIK EKRERLHMTQ KEFADALGLS KYGDRTIRRW ERGETKPTGA ELKAVIDFPD 

        70         80         90        100        110        120 
TPPYPNNENG RYRMIDLFAG IGGTRLGFHQ TNAVNVVFSS EWDKFAQKTY HANYGDFPDG 

       130        140        150        160        170        180 
DITKIDEKDI PDHEILVGGF PCVAFSQAGL KKGFNDTRGT LFFDIARIIK EKKPHAFLLE 

       190        200        210        220        230        240 
NVKNLLGHDK GRTFSIIKNT LEELNYTVYY NIFAAKDFGV PQNRERIYIV GFNKEKVRNH 

       250        260        270        280        290        300 
EHFTFPTPLK TKTRVGDILE KSVDNKYTLS DALWNGHQRR KLVNAAAGKG FGYGLFNENS 

       310        320        330        340        350        360 
PYTNTISARY YKDGSEILIE QKGSNPRKIT PREASRLQGF PSDFIIPVSD TQAYKQFGNS 

       370 
VAVPVINAIA EKIISTLDS 

« Hide

References

[1]"Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase."
Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.
Gene 124:13-19(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86545 Genomic DNA. Translation: AAA98279.1.
PIRJT0744.

3D structure databases

ProteinModelPortalP34879.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3509. M.SsoII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SMARTSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS2_SHISO
AccessionPrimary (citable) accession number: P34879
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries