Reviewed, UniProtKB/Swiss-Prot P34879 (MTS2_SHISO)

Names and origin

Protein names

Recommended name:
    Modification methylase SsoII
      Short name=M.SsoII
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase SsoII

Gene names

Name:

ssoIIM

Encoded on

Plasmid P4

Organism

Shigella sonnei

Taxonomic identifier

624 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SsoII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 HTH cro/C1-type DNA-binding domain.

Ontologies

Keywords
Biological process	Restriction system Transcription Transcription regulation
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Modification methylase SsoII		PRO_0000087906
Regions
	Domain	9 – 66	58	HTH cro/C1-type
Sites
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34879-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 97440A79FC6AB275	FASTA	379	42,890
	10 20 30 40 50 60 MTDNIAATIK EKRERLHMTQ KEFADALGLS KYGDRTIRRW ERGETKPTGA ELKAVIDFPD 70 80 90 100 110 120 TPPYPNNENG RYRMIDLFAG IGGTRLGFHQ TNAVNVVFSS EWDKFAQKTY HANYGDFPDG 130 140 150 160 170 180 DITKIDEKDI PDHEILVGGF PCVAFSQAGL KKGFNDTRGT LFFDIARIIK EKKPHAFLLE 190 200 210 220 230 240 NVKNLLGHDK GRTFSIIKNT LEELNYTVYY NIFAAKDFGV PQNRERIYIV GFNKEKVRNH 250 260 270 280 290 300 EHFTFPTPLK TKTRVGDILE KSVDNKYTLS DALWNGHQRR KLVNAAAGKG FGYGLFNENS 310 320 330 340 350 360 PYTNTISARY YKDGSEILIE QKGSNPRKIT PREASRLQGF PSDFIIPVSD TQAYKQFGNS 370 VAVPVINAIA EKIISTLDS

References

[1]

"Analysis of the nucleotide and derived amino acid sequences of the SsoII restriction endonuclease and methyltransferase." Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I. Gene 124:13-19(1993) [PubMed: 7916706] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 47.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86545 Genomic DNA. Translation: AAA98279.1.
PIR	JT0744.
3D structure databases
HSSP	HSSP built from PDB template 1FJX based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	3509. M.SsoII.
Enzyme and pathway databases
BRENDA	2.1.1.37. 74546.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR001387. HTH_3. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. PF01381. HTH_3. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00530. HTH_XRE. 1 hit. [Graphical view]
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. PS50943. HTH_CROC1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS2_SHISO

Accession

Primary (citable) accession number: P34879

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	September 22, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)