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P34878

- MTSB_LACLC

UniProt

P34878 - MTSB_LACLC

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Protein
Modification methylase ScrFIB
Gene
scrFIBM
Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3682. M2.ScrFI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ScrFIB (EC:2.1.1.37)
Short name:
M.ScrFI-B
Short name:
M.ScrFIB
Alternative name(s):
Cytosine-specific methyltransferase ScrFIB
Gene namesi
Name:scrFIBM
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Modification methylase ScrFIB
PRO_0000087893Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP34878.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 360359SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34878-1 [UniParc]FASTAAdd to Basket

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MLRVFEAFAG YGSQRLALIK ANIPHEIVGI SEIEGDVLLS YSAIHENLLE    50
ERNKNIKLTD DEMRDYLKNI NIPLDYKTFE NRADKLGGQK LKDMYIANKL 100
NKNFGDIRSI DPKKLPDFDF FTYSFPCQDI SVAGYQNGLV ADSGTRSSLL 150
WECCKIIEHK KPKYLMMENV KNLVGKNHKV NFNKFLLYLE SLGYTNYWDI 200
LNARDFGIPQ NRERVFCISI LNPNEDFTFP QKQNLTLSMN DLLEENVSEK 250
FYLKNNQVSD EPILQDYIYC LDSNYWKGTF LKDFLSKKRR QLVSGKVRPD 300
GKYPARRLTP RETWRFMGVE DTNFDKASIL VSNTSLYKQS GNSIVVPVLE 350
SLFKELFKSQ 360
Length:360
Mass (Da):41,844
Last modified:February 1, 1994 - v1
Checksum:iB51D60F72A22A7D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12227 Unassigned DNA. Translation: AAA16838.1.
U89998 Genomic DNA. Translation: AAB66694.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12227 Unassigned DNA. Translation: AAA16838.1 .
U89998 Genomic DNA. Translation: AAB66694.1 .

3D structure databases

ProteinModelPortali P34878.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3682. M2.ScrFI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the gene encoding a second ScrFI m5C methyltransferase of Lactococcus lactis."
    Twomey D.P., Davis R., Daly C., Fitzgerald G.F.
    Gene 136:205-209(1993)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UC503.

Entry informationi

Entry nameiMTSB_LACLC
AccessioniPrimary (citable) accession number: P34878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi