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P34878

- MTSB_LACLC

UniProt

P34878 - MTSB_LACLC

Protein

Modification methylase ScrFIB

Gene

scrFIBM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei127 – 1271PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3682. M2.ScrFI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase ScrFIB (EC:2.1.1.37)
    Short name:
    M.ScrFI-B
    Short name:
    M.ScrFIB
    Alternative name(s):
    Cytosine-specific methyltransferase ScrFIB
    Gene namesi
    Name:scrFIBM
    OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
    Taxonomic identifieri1359 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Modification methylase ScrFIBPRO_0000087893Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP34878.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 360359SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34878-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRVFEAFAG YGSQRLALIK ANIPHEIVGI SEIEGDVLLS YSAIHENLLE    50
    ERNKNIKLTD DEMRDYLKNI NIPLDYKTFE NRADKLGGQK LKDMYIANKL 100
    NKNFGDIRSI DPKKLPDFDF FTYSFPCQDI SVAGYQNGLV ADSGTRSSLL 150
    WECCKIIEHK KPKYLMMENV KNLVGKNHKV NFNKFLLYLE SLGYTNYWDI 200
    LNARDFGIPQ NRERVFCISI LNPNEDFTFP QKQNLTLSMN DLLEENVSEK 250
    FYLKNNQVSD EPILQDYIYC LDSNYWKGTF LKDFLSKKRR QLVSGKVRPD 300
    GKYPARRLTP RETWRFMGVE DTNFDKASIL VSNTSLYKQS GNSIVVPVLE 350
    SLFKELFKSQ 360
    Length:360
    Mass (Da):41,844
    Last modified:February 1, 1994 - v1
    Checksum:iB51D60F72A22A7D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12227 Unassigned DNA. Translation: AAA16838.1.
    U89998 Genomic DNA. Translation: AAB66694.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12227 Unassigned DNA. Translation: AAA16838.1 .
    U89998 Genomic DNA. Translation: AAB66694.1 .

    3D structure databases

    ProteinModelPortali P34878.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3682. M2.ScrFI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the gene encoding a second ScrFI m5C methyltransferase of Lactococcus lactis."
      Twomey D.P., Davis R., Daly C., Fitzgerald G.F.
      Gene 136:205-209(1993)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: UC503.

    Entry informationi

    Entry nameiMTSB_LACLC
    AccessioniPrimary (citable) accession number: P34878
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3