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P34878 (MTSB_LACLC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase ScrFIB

Short name=M.ScrFI-B
Short name=M.ScrFIB
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase ScrFIB
Gene names
Name:scrFIBM
OrganismLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifier1359 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Modification methylase ScrFIB
PRO_0000087893

Regions

Domain2 – 360359SAM-dependent MTase C5-type

Sites

Active site1271 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34878 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: B51D60F72A22A7D7

FASTA36041,844
        10         20         30         40         50         60 
MLRVFEAFAG YGSQRLALIK ANIPHEIVGI SEIEGDVLLS YSAIHENLLE ERNKNIKLTD 

        70         80         90        100        110        120 
DEMRDYLKNI NIPLDYKTFE NRADKLGGQK LKDMYIANKL NKNFGDIRSI DPKKLPDFDF 

       130        140        150        160        170        180 
FTYSFPCQDI SVAGYQNGLV ADSGTRSSLL WECCKIIEHK KPKYLMMENV KNLVGKNHKV 

       190        200        210        220        230        240 
NFNKFLLYLE SLGYTNYWDI LNARDFGIPQ NRERVFCISI LNPNEDFTFP QKQNLTLSMN 

       250        260        270        280        290        300 
DLLEENVSEK FYLKNNQVSD EPILQDYIYC LDSNYWKGTF LKDFLSKKRR QLVSGKVRPD 

       310        320        330        340        350        360 
GKYPARRLTP RETWRFMGVE DTNFDKASIL VSNTSLYKQS GNSIVVPVLE SLFKELFKSQ 

« Hide

References

[1]"Sequence of the gene encoding a second ScrFI m5C methyltransferase of Lactococcus lactis."
Twomey D.P., Davis R., Daly C., Fitzgerald G.F.
Gene 136:205-209(1993)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: UC503.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12227 Unassigned DNA. Translation: AAA16838.1.
U89998 Genomic DNA. Translation: AAB66694.1.

3D structure databases

ProteinModelPortalP34878.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3682. M2.ScrFI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTSB_LACLC
AccessionPrimary (citable) accession number: P34878
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries