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Protein

Modification methylase ScrFIB

Gene

scrFIBM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3682 M2.ScrFI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ScrFIB (EC:2.1.1.37)
Short name:
M.ScrFI-B
Short name:
M.ScrFIB
Alternative name(s):
Cytosine-specific methyltransferase ScrFIB
Gene namesi
Name:scrFIBM
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878931 – 360Modification methylase ScrFIBAdd BLAST360

Structurei

3D structure databases

ProteinModelPortaliP34878
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 360SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST359

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 2 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P34878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVFEAFAG YGSQRLALIK ANIPHEIVGI SEIEGDVLLS YSAIHENLLE
60 70 80 90 100
ERNKNIKLTD DEMRDYLKNI NIPLDYKTFE NRADKLGGQK LKDMYIANKL
110 120 130 140 150
NKNFGDIRSI DPKKLPDFDF FTYSFPCQDI SVAGYQNGLV ADSGTRSSLL
160 170 180 190 200
WECCKIIEHK KPKYLMMENV KNLVGKNHKV NFNKFLLYLE SLGYTNYWDI
210 220 230 240 250
LNARDFGIPQ NRERVFCISI LNPNEDFTFP QKQNLTLSMN DLLEENVSEK
260 270 280 290 300
FYLKNNQVSD EPILQDYIYC LDSNYWKGTF LKDFLSKKRR QLVSGKVRPD
310 320 330 340 350
GKYPARRLTP RETWRFMGVE DTNFDKASIL VSNTSLYKQS GNSIVVPVLE
360
SLFKELFKSQ
Length:360
Mass (Da):41,844
Last modified:February 1, 1994 - v1
Checksum:iB51D60F72A22A7D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12227 Unassigned DNA Translation: AAA16838.1
U89998 Genomic DNA Translation: AAB66694.1
RefSeqiWP_015081858.1, NZ_CP015894.1

Similar proteinsi

Entry informationi

Entry nameiMTSB_LACLC
AccessioniPrimary (citable) accession number: P34878
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health