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Protein

Modification methylase ScrFIB

Gene

scrFIBM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3682. M2.ScrFI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ScrFIB (EC:2.1.1.37)
Short name:
M.ScrFI-B
Short name:
M.ScrFIB
Alternative name(s):
Cytosine-specific methyltransferase ScrFIB
Gene namesi
Name:scrFIBM
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Modification methylase ScrFIBPRO_0000087893Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP34878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 360359SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVFEAFAG YGSQRLALIK ANIPHEIVGI SEIEGDVLLS YSAIHENLLE
60 70 80 90 100
ERNKNIKLTD DEMRDYLKNI NIPLDYKTFE NRADKLGGQK LKDMYIANKL
110 120 130 140 150
NKNFGDIRSI DPKKLPDFDF FTYSFPCQDI SVAGYQNGLV ADSGTRSSLL
160 170 180 190 200
WECCKIIEHK KPKYLMMENV KNLVGKNHKV NFNKFLLYLE SLGYTNYWDI
210 220 230 240 250
LNARDFGIPQ NRERVFCISI LNPNEDFTFP QKQNLTLSMN DLLEENVSEK
260 270 280 290 300
FYLKNNQVSD EPILQDYIYC LDSNYWKGTF LKDFLSKKRR QLVSGKVRPD
310 320 330 340 350
GKYPARRLTP RETWRFMGVE DTNFDKASIL VSNTSLYKQS GNSIVVPVLE
360
SLFKELFKSQ
Length:360
Mass (Da):41,844
Last modified:February 1, 1994 - v1
Checksum:iB51D60F72A22A7D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12227 Unassigned DNA. Translation: AAA16838.1.
U89998 Genomic DNA. Translation: AAB66694.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12227 Unassigned DNA. Translation: AAA16838.1.
U89998 Genomic DNA. Translation: AAB66694.1.

3D structure databases

ProteinModelPortaliP34878.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3682. M2.ScrFI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the gene encoding a second ScrFI m5C methyltransferase of Lactococcus lactis."
    Twomey D.P., Davis R., Daly C., Fitzgerald G.F.
    Gene 136:205-209(1993)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UC503.

Entry informationi

Entry nameiMTSB_LACLC
AccessioniPrimary (citable) accession number: P34878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.