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P34877

- MTSA_LACLC

UniProt

P34877 - MTSA_LACLC

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Protein
Modification methylase ScrFIA
Gene
scrFIAM
Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi3681. M1.ScrFI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ScrFIA (EC:2.1.1.37)
Short name:
M.ScrFI-A
Short name:
M.ScrFIA
Alternative name(s):
Cytosine-specific methyltransferase ScrFIA
Gene namesi
Name:scrFIAM
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Modification methylase ScrFIA
PRO_0000087892Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP34877.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 7156HTH cro/C1-type
Add
BLAST
Domaini79 – 387309SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34877-1 [UniParc]FASTAAdd to Basket

« Hide

MTTISRNTGT EISIMIKEKR LRLNMTQKEL ADAVGMSKNG DRTIRRWENG    50
ETCPSQLEIS AILRFPEIAP FENRKTAKYK MIDLFAGIGG TRLGFHQTEK 100
VKSVFSSEID KFAIKTYKAN FGDEPHGDIT KIDEKDIPDH DILVGGFPCQ 150
AFSQAGKKLG FDDTRGTLFF EIARIIKEKR PKAFLLENVK NLKTHDKGRT 200
FKTILNTLEE LDYEVHTALF KARDFGLPQN RERIYIVGFD RKSISNYSDF 250
QMPTPLQEKT RVGNILESVV DDKYTISDKL WDGHQRRKTE NKKNGKGFGY 300
TLFNQDSEYT NTLSARYYKD GSEILIEQKN KNPRKITPRE AARLQGFPEN 350
FIIPVSDTQA YKEFGNSVAV PTIHAIAEKM LEVLEKSKK 389
Length:389
Mass (Da):44,542
Last modified:February 1, 1994 - v1
Checksum:i6F5FAFFD9973CC02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87289 Genomic DNA. Translation: AAA25220.1.
U89998 Genomic DNA. Translation: AAB66696.1.
PIRiA48966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87289 Genomic DNA. Translation: AAA25220.1 .
U89998 Genomic DNA. Translation: AAB66696.1 .
PIRi A48966.

3D structure databases

ProteinModelPortali P34877.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3681. M1.ScrFI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SMARTi SM00530. HTH_XRE. 1 hit.
[Graphical view ]
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes."
    Davis R., van der Lelie D., Mercenier A., Daly C., Fitzgerald G.F.
    Appl. Environ. Microbiol. 59:777-785(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UC503.

Entry informationi

Entry nameiMTSA_LACLC
AccessioniPrimary (citable) accession number: P34877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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