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P34877

- MTSA_LACLC

UniProt

P34877 - MTSA_LACLC

Protein

Modification methylase ScrFIA

Gene

scrFIAM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei149 – 1491PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3681. M1.ScrFI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase ScrFIA (EC:2.1.1.37)
    Short name:
    M.ScrFI-A
    Short name:
    M.ScrFIA
    Alternative name(s):
    Cytosine-specific methyltransferase ScrFIA
    Gene namesi
    Name:scrFIAM
    OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
    Taxonomic identifieri1359 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 389389Modification methylase ScrFIAPRO_0000087892Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP34877.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 7156HTH cro/C1-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini79 – 387309SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    PF01381. HTH_3. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34877-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTISRNTGT EISIMIKEKR LRLNMTQKEL ADAVGMSKNG DRTIRRWENG    50
    ETCPSQLEIS AILRFPEIAP FENRKTAKYK MIDLFAGIGG TRLGFHQTEK 100
    VKSVFSSEID KFAIKTYKAN FGDEPHGDIT KIDEKDIPDH DILVGGFPCQ 150
    AFSQAGKKLG FDDTRGTLFF EIARIIKEKR PKAFLLENVK NLKTHDKGRT 200
    FKTILNTLEE LDYEVHTALF KARDFGLPQN RERIYIVGFD RKSISNYSDF 250
    QMPTPLQEKT RVGNILESVV DDKYTISDKL WDGHQRRKTE NKKNGKGFGY 300
    TLFNQDSEYT NTLSARYYKD GSEILIEQKN KNPRKITPRE AARLQGFPEN 350
    FIIPVSDTQA YKEFGNSVAV PTIHAIAEKM LEVLEKSKK 389
    Length:389
    Mass (Da):44,542
    Last modified:February 1, 1994 - v1
    Checksum:i6F5FAFFD9973CC02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87289 Genomic DNA. Translation: AAA25220.1.
    U89998 Genomic DNA. Translation: AAB66696.1.
    PIRiA48966.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87289 Genomic DNA. Translation: AAA25220.1 .
    U89998 Genomic DNA. Translation: AAB66696.1 .
    PIRi A48966.

    3D structure databases

    ProteinModelPortali P34877.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3681. M1.ScrFI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    PF01381. HTH_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes."
      Davis R., van der Lelie D., Mercenier A., Daly C., Fitzgerald G.F.
      Appl. Environ. Microbiol. 59:777-785(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: UC503.

    Entry informationi

    Entry nameiMTSA_LACLC
    AccessioniPrimary (citable) accession number: P34877
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3