Reviewed, UniProtKB/Swiss-Prot P34877 (MTSA_LACLC)

Names and origin

Protein names

Recommended name:
    Modification methylase ScrFIA
      Short name=M.ScrFIA
      Short name=M.ScrFI-A
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase ScrFIA

Gene names

Name:

scrFIAM

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 HTH cro/C1-type DNA-binding domain.

Ontologies

Keywords
Biological process	Restriction system Transcription Transcription regulation
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Modification methylase ScrFIA		PRO_0000087892
Regions
	Domain	16 – 71	56	HTH cro/C1-type
Sites
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34877-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 6F5FAFFD9973CC02	FASTA	389	44,542
	10 20 30 40 50 60 MTTISRNTGT EISIMIKEKR LRLNMTQKEL ADAVGMSKNG DRTIRRWENG ETCPSQLEIS 70 80 90 100 110 120 AILRFPEIAP FENRKTAKYK MIDLFAGIGG TRLGFHQTEK VKSVFSSEID KFAIKTYKAN 130 140 150 160 170 180 FGDEPHGDIT KIDEKDIPDH DILVGGFPCQ AFSQAGKKLG FDDTRGTLFF EIARIIKEKR 190 200 210 220 230 240 PKAFLLENVK NLKTHDKGRT FKTILNTLEE LDYEVHTALF KARDFGLPQN RERIYIVGFD 250 260 270 280 290 300 RKSISNYSDF QMPTPLQEKT RVGNILESVV DDKYTISDKL WDGHQRRKTE NKKNGKGFGY 310 320 330 340 350 360 TLFNQDSEYT NTLSARYYKD GSEILIEQKN KNPRKITPRE AARLQGFPEN FIIPVSDTQA 370 380 YKEFGNSVAV PTIHAIAEKM LEVLEKSKK

References

[1]

"ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes." Davis R., van der Lelie D., Mercenier A., Daly C., Fitzgerald G.F. Appl. Environ. Microbiol. 59:777-785(1993) [PubMed: 8481004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UC503.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M87289 Genomic DNA. Translation: AAA25220.1. U89998 Genomic DNA. Translation: AAB66696.1.
PIR	A48966.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	3681. M1.ScrFI.
Enzyme and pathway databases
BRENDA	2.1.1.37. 289716.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR001387. HTH_3. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. PF01381. HTH_3. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00530. HTH_XRE. 1 hit. [Graphical view]
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. PS50943. HTH_CROC1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTSA_LACLC

Accession

Primary (citable) accession number: P34877

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)