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P34877 (MTSA_LACLC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase ScrFIA

Short name=M.ScrFI-A
Short name=M.ScrFIA
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase ScrFIA
Gene names
Name:scrFIAM
OrganismLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifier1359 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 HTH cro/C1-type DNA-binding domain.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Modification methylase ScrFIA
PRO_0000087892

Regions

Domain16 – 7156HTH cro/C1-type
Domain79 – 387309SAM-dependent MTase C5-type

Sites

Active site1491 By similarity

Sequences

Sequence LengthMass (Da)Tools
P34877 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 6F5FAFFD9973CC02

FASTA38944,542
        10         20         30         40         50         60 
MTTISRNTGT EISIMIKEKR LRLNMTQKEL ADAVGMSKNG DRTIRRWENG ETCPSQLEIS 

        70         80         90        100        110        120 
AILRFPEIAP FENRKTAKYK MIDLFAGIGG TRLGFHQTEK VKSVFSSEID KFAIKTYKAN 

       130        140        150        160        170        180 
FGDEPHGDIT KIDEKDIPDH DILVGGFPCQ AFSQAGKKLG FDDTRGTLFF EIARIIKEKR 

       190        200        210        220        230        240 
PKAFLLENVK NLKTHDKGRT FKTILNTLEE LDYEVHTALF KARDFGLPQN RERIYIVGFD 

       250        260        270        280        290        300 
RKSISNYSDF QMPTPLQEKT RVGNILESVV DDKYTISDKL WDGHQRRKTE NKKNGKGFGY 

       310        320        330        340        350        360 
TLFNQDSEYT NTLSARYYKD GSEILIEQKN KNPRKITPRE AARLQGFPEN FIIPVSDTQA 

       370        380 
YKEFGNSVAV PTIHAIAEKM LEVLEKSKK 

« Hide

References

[1]"ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes."
Davis R., van der Lelie D., Mercenier A., Daly C., Fitzgerald G.F.
Appl. Environ. Microbiol. 59:777-785(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: UC503.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M87289 Genomic DNA. Translation: AAA25220.1.
U89998 Genomic DNA. Translation: AAB66696.1.
PIRA48966.

3D structure databases

ProteinModelPortalP34877.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3681. M1.ScrFI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SMARTSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTSA_LACLC
AccessionPrimary (citable) accession number: P34877
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries