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Protein

Modification methylase ScrFIA

Gene

scrFIAM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processRestriction system, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3681 M1.ScrFI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ScrFIA (EC:2.1.1.37)
Short name:
M.ScrFI-A
Short name:
M.ScrFIA
Alternative name(s):
Cytosine-specific methyltransferase ScrFIA
Gene namesi
Name:scrFIAM
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878921 – 389Modification methylase ScrFIAAdd BLAST389

Structurei

3D structure databases

ProteinModelPortaliP34877
SMRiP34877
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 71HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST56
Domaini79 – 387SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST309

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR001387 Cro/C1-type_HTH
IPR010982 Lambda_DNA-bd_dom_sf
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PF01381 HTH_3, 1 hit
PRINTSiPR00105 C5METTRFRASE
SMARTiView protein in SMART
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS50943 HTH_CROC1, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P34877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTISRNTGT EISIMIKEKR LRLNMTQKEL ADAVGMSKNG DRTIRRWENG
60 70 80 90 100
ETCPSQLEIS AILRFPEIAP FENRKTAKYK MIDLFAGIGG TRLGFHQTEK
110 120 130 140 150
VKSVFSSEID KFAIKTYKAN FGDEPHGDIT KIDEKDIPDH DILVGGFPCQ
160 170 180 190 200
AFSQAGKKLG FDDTRGTLFF EIARIIKEKR PKAFLLENVK NLKTHDKGRT
210 220 230 240 250
FKTILNTLEE LDYEVHTALF KARDFGLPQN RERIYIVGFD RKSISNYSDF
260 270 280 290 300
QMPTPLQEKT RVGNILESVV DDKYTISDKL WDGHQRRKTE NKKNGKGFGY
310 320 330 340 350
TLFNQDSEYT NTLSARYYKD GSEILIEQKN KNPRKITPRE AARLQGFPEN
360 370 380
FIIPVSDTQA YKEFGNSVAV PTIHAIAEKM LEVLEKSKK
Length:389
Mass (Da):44,542
Last modified:February 1, 1994 - v1
Checksum:i6F5FAFFD9973CC02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87289 Genomic DNA Translation: AAA25220.1
U89998 Genomic DNA Translation: AAB66696.1
PIRiA48966
RefSeqiWP_041931724.1, NZ_CP015894.1

Similar proteinsi

Entry informationi

Entry nameiMTSA_LACLC
AccessioniPrimary (citable) accession number: P34877
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health