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P34810

- CD68_HUMAN

UniProt

P34810 - CD68_HUMAN

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Protein

Macrosialin

Gene

CD68

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

GO - Biological processi

  1. cellular response to organic substance Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Macrosialin
Alternative name(s):
Gp110
CD_antigen: CD68
Gene namesi
Name:CD68
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:1693. CD68.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 319298ExtracellularSequence AnalysisAdd
BLAST
Transmembranei320 – 34425HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini345 – 35410CytoplasmicPROSITE-ProRule annotation

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. integral component of membrane Source: ProtInc
  3. lysosome Source: UniProtKB-KW
  4. membrane Source: ProtInc
  5. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26232.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 354333MacrosialinPRO_0000017102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi169 ↔ 207PROSITE-ProRule annotation
Glycosylationi199 – 1991N-linked (GlcNAc...)1 Publication
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi261 – 2611N-linked (GlcNAc...)2 Publications
Disulfide bondi277 ↔ 314PROSITE-ProRule annotation
Glycosylationi279 – 2791N-linked (GlcNAc...)1 Publication

Post-translational modificationi

N- and O-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP34810.
PaxDbiP34810.
PRIDEiP34810.

PTM databases

PhosphoSiteiP34810.

Expressioni

Tissue specificityi

Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.1 Publication

Gene expression databases

BgeeiP34810.
CleanExiHS_CD68.
ExpressionAtlasiP34810. baseline.
GenevestigatoriP34810.

Organism-specific databases

HPAiCAB000051.
CAB000066.
HPA048982.

Interactioni

Protein-protein interaction databases

BioGridi107406. 15 interactions.
IntActiP34810. 3 interactions.
MINTiMINT-4824093.
STRINGi9606.ENSP00000250092.

Structurei

3D structure databases

ProteinModelPortaliP34810.
SMRiP34810. Positions 156-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati70 – 99301Add
BLAST
Repeati100 – 129302Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 140118Mucin-likeAdd
BLAST
Regioni70 – 129602 X 30 AA tandem repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi140 – 15213Pro-rich (hinge)Add
BLAST

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG244352.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000070034.
HOVERGENiHBG005287.
InParanoidiP34810.
KOiK06501.
OMAiGNDCPHK.
OrthoDBiEOG7ZD1VH.
PhylomeDBiP34810.
TreeFamiTF316339.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P34810-1) [UniParc]FASTAAdd to Basket

Also known as: CD68.1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLAVLFSGA LLGLLAAQGT GNDCPHKKSA TLLPSFTVTP TVTESTGTTS
60 70 80 90 100
HRTTKSHKTT THRTTTTGTT SHGPTTATHN PTTTSHGNVT VHPTSNSTAT
110 120 130 140 150
SQGPSTATHS PATTSHGNAT VHPTSNSTAT SPGFTSSAHP EPPPPSPSPS
160 170 180 190 200
PTSKETIGDY TWTNGSQPCV HLQAQIQIRV MYTTQGGGEA WGISVLNPNK
210 220 230 240 250
TKVQGSCEGA HPHLLLSFPY GHLSFGFMQD LQQKVVYLSY MAVEYNVSFP
260 270 280 290 300
HAAQWTFSAQ NASLRDLQAP LGQSFSCSNS SIILSPAVHL DLLSLRLQAA
310 320 330 340 350
QLPHTGVFGQ SFSCPSDRSI LLPLIIGLIL LGLLALVLIA FCIIRRRPSA

YQAL
Length:354
Mass (Da):37,408
Last modified:May 15, 2007 - v2
Checksum:i69E68D69EDE8EFB0
GO
Isoform Short (identifier: P34810-2) [UniParc]FASTAAdd to Basket

Also known as: CD68.2

The sequence of this isoform differs from the canonical sequence as follows:
     17-43: Missing.
     83-112: Missing.

Show »
Length:297
Mass (Da):31,738
Checksum:iB0A78BF5F01BC0B0
GO
Isoform 3 (identifier: P34810-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-43: Missing.

Show »
Length:327
Mass (Da):34,654
Checksum:i8AD19346F7827330
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951S → G in BAD96234. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti254 – 2541Q → K.1 Publication
Corresponds to variant rs25679 [ dbSNP | Ensembl ].
VAR_016144
Natural varianti329 – 3291I → T.
Corresponds to variant rs35452170 [ dbSNP | Ensembl ].
VAR_049725
Natural varianti340 – 3401A → T.
Corresponds to variant rs17607 [ dbSNP | Ensembl ].
VAR_016145
Natural varianti350 – 3501A → T.
Corresponds to variant rs9901675 [ dbSNP | Ensembl ].
VAR_049726

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei17 – 4327Missing in isoform Short and isoform 3. 2 PublicationsVSP_003041Add
BLAST
Alternative sequencei83 – 11230Missing in isoform Short. 1 PublicationVSP_003042Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57235 mRNA. Translation: AAB25811.1.
BT009923 mRNA. Translation: AAP88925.1.
AK290238 mRNA. Translation: BAF82927.1.
AK222492 mRNA. Translation: BAD96212.1.
AK222514 mRNA. Translation: BAD96234.1.
AK301223 mRNA. Translation: BAG62796.1.
AC016876 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90165.1.
BC015557 mRNA. Translation: AAH15557.1.
AF060540 Genomic DNA. Translation: AAC70006.1.
CCDSiCCDS11114.1. [P34810-1]
CCDS58512.1. [P34810-3]
PIRiA48931.
RefSeqiNP_001035148.1. NM_001040059.1. [P34810-3]
NP_001242.2. NM_001251.2. [P34810-1]
UniGeneiHs.647419.

Genome annotation databases

EnsembliENST00000250092; ENSP00000250092; ENSG00000129226. [P34810-1]
ENST00000380498; ENSP00000369867; ENSG00000129226. [P34810-3]
GeneIDi968.
KEGGihsa:968.
UCSCiuc002ghu.3. human.
uc002ghv.3. human. [P34810-1]

Polymorphism databases

DMDMi147744552.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57235 mRNA. Translation: AAB25811.1 .
BT009923 mRNA. Translation: AAP88925.1 .
AK290238 mRNA. Translation: BAF82927.1 .
AK222492 mRNA. Translation: BAD96212.1 .
AK222514 mRNA. Translation: BAD96234.1 .
AK301223 mRNA. Translation: BAG62796.1 .
AC016876 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90165.1 .
BC015557 mRNA. Translation: AAH15557.1 .
AF060540 Genomic DNA. Translation: AAC70006.1 .
CCDSi CCDS11114.1. [P34810-1 ]
CCDS58512.1. [P34810-3 ]
PIRi A48931.
RefSeqi NP_001035148.1. NM_001040059.1. [P34810-3 ]
NP_001242.2. NM_001251.2. [P34810-1 ]
UniGenei Hs.647419.

3D structure databases

ProteinModelPortali P34810.
SMRi P34810. Positions 156-316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107406. 15 interactions.
IntActi P34810. 3 interactions.
MINTi MINT-4824093.
STRINGi 9606.ENSP00000250092.

PTM databases

PhosphoSitei P34810.

Polymorphism databases

DMDMi 147744552.

Proteomic databases

MaxQBi P34810.
PaxDbi P34810.
PRIDEi P34810.

Protocols and materials databases

DNASUi 968.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000250092 ; ENSP00000250092 ; ENSG00000129226 . [P34810-1 ]
ENST00000380498 ; ENSP00000369867 ; ENSG00000129226 . [P34810-3 ]
GeneIDi 968.
KEGGi hsa:968.
UCSCi uc002ghu.3. human.
uc002ghv.3. human. [P34810-1 ]

Organism-specific databases

CTDi 968.
GeneCardsi GC17P007482.
HGNCi HGNC:1693. CD68.
HPAi CAB000051.
CAB000066.
HPA048982.
MIMi 153634. gene.
neXtProti NX_P34810.
PharmGKBi PA26232.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG244352.
GeneTreei ENSGT00530000063068.
HOGENOMi HOG000070034.
HOVERGENi HBG005287.
InParanoidi P34810.
KOi K06501.
OMAi GNDCPHK.
OrthoDBi EOG7ZD1VH.
PhylomeDBi P34810.
TreeFami TF316339.

Miscellaneous databases

ChiTaRSi CD68. human.
GenomeRNAii 968.
NextBioi 4050.
PROi P34810.
SOURCEi Search...

Gene expression databases

Bgeei P34810.
CleanExi HS_CD68.
ExpressionAtlasi P34810. baseline.
Genevestigatori P34810.

Family and domain databases

InterProi IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view ]
PANTHERi PTHR11506. PTHR11506. 1 hit.
Pfami PF01299. Lamp. 1 hit.
[Graphical view ]
PRINTSi PR00336. LYSASSOCTDMP.
PROSITEi PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins."
    Holness C.L., Simmons D.L.
    Blood 81:1607-1613(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), VARIANT LYS-254.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND 3).
    Tissue: Spleen and Umbilical cord blood.
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Tissue: Adipose tissue.
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Tissue: Skin.
  8. "The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13."
    Jones E., Quinn C.M., See C.G., Montgomery D.S., Ford M.J., Koelble K., Gordon S., Greaves D.R.
    Genomics 53:248-250(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
  9. "Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry."
    Kunisch E., Fuhrmann R., Roth A., Winter R., Lungershausen W., Kinne R.W.
    Ann. Rheum. Dis. 63:774-784(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: USE AS A MACROPHAGE MARKER.
  10. "CD68 is not a macrophage-specific antigen."
    Beranek J.T.
    Ann. Rheum. Dis. 64:342-343(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: USE AS A MACROPHAGE MARKER.
  11. "Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach."
    Lewandrowski U., Moebius J., Walter U., Sickmann A.
    Mol. Cell. Proteomics 5:226-233(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-261.
    Tissue: Platelet.
  12. Cited for: TISSUE SPECIFICITY.
  13. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-199; ASN-261 AND ASN-279.
    Tissue: Liver.

Entry informationi

Entry nameiCD68_HUMAN
AccessioniPrimary (citable) accession number: P34810
Secondary accession number(s): B4DVT4
, Q53HR6, Q53XI3, Q96BI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 15, 2007
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

CD68 is a commonly used marker for macrophages. However, a number of studies (PubMed:15194571, PubMed:15647451 and PubMed:18405323) have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3