P34799 (URIC2_CANLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Uricase-2 isozyme 2 EC=1.7.3.3 Alternative name(s): Urate oxidase Uricase II clone pcClNUO-02
Organism	Canavalia lineata (Beach bean) (Dolichos lineatus)
Taxonomic identifier	28957 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Canavalia

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin By similarity.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Pathway	Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Nodulation Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	nodulation Inferred from electronic annotation. Source: UniProtKB-KW purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW urate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Uricase-2 isozyme 2		PRO_0000166001
Regions
	Region	63 – 64	2	Substrate binding By similarity
	Region	237 – 238	2	Substrate binding By similarity
	Motif	299 – 301	3	Microbody targeting signal Potential
Sites
	Active site	182	1	Charge relay system By similarity
	Active site	238	1	Charge relay system By similarity
	Binding site	182	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34799 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: E90B96F7B5BD5294	FASTA	301	34,035
	10 20 30 40 50 60 MAKEIVGGFK FDQRHGKERV RVARVWKTKK GGYFIVEWRV GISLLSDCVN SYVRDDNSDI 70 80 90 100 110 120 VATDTMKNTV YAKAKECSEI LSVEDFAILL AKHFISFYKQ VTAAIVNIVE KPWERVSVDG 130 140 150 160 170 180 QPHEHGFKLG SERHTAEAIV QKSGALQLTS GIEGLSLLKT TKSGFEGFIR DKYTALPETH 190 200 210 220 230 240 ERMLATEVTA LWRYSYESLY SIPQKPLYFT DKYLEVKKVL ADTFFGPPNV GVYSPSVQNT 250 260 270 280 290 300 LYLMAKAHSS IQLKMPNIHF LPVNISNKDG PIVKFDDDVY FPTDEPHGSI QASLSRLWSK L

References

[1]	"Nucleotide sequence and expression of cDNA clones encoding uricase II in Canavalia lineata." Bang K.H., An C.S. Singmul Hakhoe Chi 36:415-423(1993) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76287 mRNA. Translation: CAA53905.1.
PIR	S38910.
3D structure databases
ProteinModelPortal	P34799.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00394; UER00650.
Family and domain databases
Gene3D	3.10.270.10. 1 hit.
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
PANTHER	PTHR10395:SF1. PTHR10395:SF1. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PIRSF	PIRSF000241. Urate_oxidase. 1 hit.
PRINTS	PR00093. URICASE.
TIGRFAMs	TIGR03383. urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC2_CANLI

Accession

Primary (citable) accession number: P34799

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	March 6, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program