Reviewed, UniProtKB/Swiss-Prot P34795 (G6PI_ARATH)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate isomerase, cytosolic
      Short name=GPI
    EC=5.3.1.9
Alternative name(s):
    Phosphoglucose isomerase
      Short name=PGI
    Phosphohexose isomerase
      Short name=PHI

Gene names

Name:	PGIC
Ordered Locus Names:	At5g42740
ORF Names:	MJB21.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Polymorphism	Was sequenced in many cultivars; Ag-0, Bl-1, Bus-1, Ci-0, Cvi-0, Dra-0, Edi-0, Hau-0, Hiroshima, In-0, Ita-0, Kas-1, Mr-0, Nok-4, Ost-0, Pog-0, Rou-0, Su-0, Ts-1 and Ws-0.
Sequence similarities	Belongs to the GPI family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Glucose-6-phosphate isomerase, cytosolic		PRO_0000180550
Sites
	Active site	361	1	Proton donor By similarity
	Active site	392	1	By similarity
	Active site	517	1	By similarity
Natural variations
	Natural variant	3	1	S → P in strain: cv. Bus-1.
	Natural variant	5	1	T → P in strain: cv. Ag-0, cv. Bus-1, cv. Dra-0, cv. Hiroshima and cv. Kas-1.
	Natural variant	36	1	A → V in strain: cv. Bus-1.
	Natural variant	73	1	S → A in strain: cv. Bl-1 and cv. In-0.
	Natural variant	95	1	S → A in strain: cv. Bl-1 and cv. In-0.
	Natural variant	99	1	V → I in strain: cv. Bus-1 and cv. Kas-1.
	Natural variant	105	1	K → E in strain: cv. Hiroshima.
	Natural variant	114	1	M → K in strain: cv. Bl-1 and cv. In-0.
	Natural variant	119	1	E → D in strain: cv. Nok-4.
	Natural variant	198	1	N → S in strain: cv. Bus-1.
	Natural variant	295	1	M → T in strain: cv. Cvi-0.
	Natural variant	355	1	I → T in strain: cv. WS-0.
	Natural variant	425	1	Missing in strain: cv. Cvi-0.
	Natural variant	431	1	L → F in strain: cv. Pog-0.
	Natural variant	432	1	M → I in strain: cv. In-0.
	Natural variant	528	1	H → Q in strain: cv. Ost-0.
Experimental info
	Sequence conflict	216	1	F → S in AAK50107. Ref.5
	Sequence conflict	216	1	F → S in AAM16223. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P34795-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: C4F5C7D8FE5F193B	FASTA	560	61,718
	10 20 30 40 50 60 MASSTALICD TEAWKDLKGH VEDIKKTHLR DLMSDANRCQ SMMMEFDGLL LDYSRQRATV 70 80 90 100 110 120 ETMDKLLNLA KASQLTEKIS RMFNGEHINS TENRSVLHVA LRAPKDAVIK ADGMNVVPEV 130 140 150 160 170 180 WNVLDKIKEF SDKIRSGSWV GATGKPLKDV IAIGIGGSFL GPLFVHTALQ TDPEALESAK 190 200 210 220 230 240 GRQLRFLANI DPVDVARNIS GLNPETTLVV VVSKTFTTAE TMLNARTLRE WITAALGASA 250 260 270 280 290 300 VAKHMVAVST NLALVEKFGI DPNNAFAFWD WVGGRYSVCS AVGVLPLSLQ YGFSMVEKFL 310 320 330 340 350 360 KGASSIDQHF QSTPFEKNIP VLLGLLSVWN VSFLGYPARA ILPYSQALEK FAPHIQQVSM 370 380 390 400 410 420 ESNGKGVSID GLPLPFETGE IDFGEPGTNG QHSFYQLIHQ GRVIPCDFIG IVKSQQPVYL 430 440 450 460 470 480 KGEVVSNHDE LMSNFFAQPD ALAYGKTPEQ LQKENVSENL IPHKTFSGNR PSLSLLLPEL 490 500 510 520 530 540 TAYNVGQLLA IYEHRVAVQG FVWGINSFDQ WGVELGKVLA TQVRKQLHSS RTQGTAPEGF 550 560 NYSTTTLLKR YLETSSEPQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of duplicate cytosolic phosphoglucose isomerase genes in Clarkia and comparison to the single gene in Arabidopsis." Thomas B.R., Ford V.S., Pichersky E., Gottlieb L.D. Genetics 135:895-905(1993) [PubMed: 8293986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"The 5' leader of plant PgiC has an intron: the leader shows both the loss and maintenance of constraints compared with introns and exons in the coding region." Gottlieb L.D., Ford V.S. Mol. Biol. Evol. 19:1613-1623(2002) [PubMed: 12200488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"DNA polymorphism in active gene and pseudogene of the cytosolic phosphoglucose isomerase (PgiC) loci in Arabidopsis halleri ssp. gemmifera." Kawabe A., Miyashita N.T. Mol. Biol. Evol. 20:1043-1050(2003) [PubMed: 12716977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Many cultivars.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69195 Genomic DNA. Translation: CAA48940.1. AJ419524 mRNA. Translation: CAD11677.1. AB044948 Genomic DNA. Translation: BAB17635.1. AB044949 Genomic DNA. Translation: BAB17636.1. AB044950 Genomic DNA. Translation: BAB17637.1. AB044951 Genomic DNA. Translation: BAB17638.1. AB044952 Genomic DNA. Translation: BAB17639.1. AB044953 Genomic DNA. Translation: BAB17640.1. AB044954 Genomic DNA. Translation: BAB17641.1. AB044955 Genomic DNA. Translation: BAB17642.1. AB044956 Genomic DNA. Translation: BAB17643.1. AB044957 Genomic DNA. Translation: BAB17644.1. AB044958 Genomic DNA. Translation: BAB17645.1. AB044959 Genomic DNA. Translation: BAB17646.1. AB044960 Genomic DNA. Translation: BAB17647.1. AB044961 Genomic DNA. Translation: BAB17648.1. AB044962 Genomic DNA. Translation: BAB17649.1. AB044963 Genomic DNA. Translation: BAB17650.1. AB044964 Genomic DNA. Translation: BAB17651.1. AB044965 Genomic DNA. Translation: BAB17652.1. AB044966 Genomic DNA. Translation: BAB17653.1. AB044967 Genomic DNA. Translation: BAB17654.1. AB007647 Genomic DNA. Translation: BAB10630.1. AF372970 mRNA. Translation: AAK50107.1. AY093962 mRNA. Translation: AAM16223.1.
IPI	IPI00531102.
PIR	S41808.
RefSeq	NP_199088.1.
UniGene	At.20998
3D structure databases
SMR	P34795. Positions 8-554.
ModBase	Search...
Proteomic databases
PRIDE	P34795.
Genome annotation databases
GeneID	834283.
GenomeReviews	Gene locus AT5G42740 in contig BA000015_GR.
KEGG	ath:AT5G42740.
NMPDR	fig|3702.1.peg.25997.
Organism-specific databases
TAIR	At5g42740.
Phylogenomic databases
InParanoid	P34795.
OMA	GPKIVSQ.
PhylomeDB	P34795.
Enzyme and pathway databases
BRENDA	5.3.1.9. 302.
Gene expression databases
ArrayExpress	P34795.
Genevestigator	P34795.
GermOnline	AT5G42740. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001672. G6P_Isomerase. IPR018189. Phosphoglucose_isomerase_CS. [Graphical view]
PANTHER	PTHR11469. G6P_Isomerase. 1 hit.
Pfam	PF00342. PGI. 1 hit. [Graphical view]
PRINTS	PR00662. G6PISOMERASE.
PROSITE	PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit. PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit. PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PI_ARATH

Accession

Primary (citable) accession number: P34795
Secondary accession number(s): Q546J1 , Q94JT1, Q9FE90, Q9FXM6, Q9FXM7, Q9FXM8, Q9FXM9, Q9FXN0, Q9FXN1, Q9FXN2, Q9FXN3, Q9FXN4, Q9FXN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)