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P34795 (G6PI_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate isomerase, cytosolic

Short name=GPI
EC=5.3.1.9
Alternative name(s):
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI
Gene names
Name:PGIC
Ordered Locus Names:At5g42740
ORF Names:MJB21.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_00473

Enzyme regulation

Inhibited by glycerol-3-P (G3P). Ref.7

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_00473

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00473

Subcellular location

Cytoplasm HAMAP-Rule MF_00473.

Polymorphism

Was sequenced in many cultivars; Ag-0, Bl-1, Bus-1, Ci-0, Cvi-0, Dra-0, Edi-0, Hau-0, Hiroshima, In-0, Ita-0, Kas-1, Mr-0, Nok-4, Ost-0, Pog-0, Rou-0, Su-0, Ts-1 and Ws-0. HAMAP-Rule MF_00473

Sequence similarities

Belongs to the GPI family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   Molecular functionIsomerase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to fungus, incompatible interaction

Inferred from direct assay PubMed 19857612. Source: TAIR

gluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

response to cadmium ion

Inferred from expression pattern PubMed 16502469. Source: TAIR

   Cellular_componentcytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

   Molecular_functionglucose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 560559Glucose-6-phosphate isomerase, cytosolic HAMAP-Rule MF_00473
PRO_0000180550

Sites

Active site3611Proton donor By similarity
Active site3921 By similarity
Active site5171 By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.8

Natural variations

Natural variant31S → P in strain: cv. Bus-1.
Natural variant51T → P in strain: cv. Ag-0, cv. Bus-1, cv. Dra-0, cv. Hiroshima and cv. Kas-1.
Natural variant361A → V in strain: cv. Bus-1.
Natural variant731S → A in strain: cv. Bl-1 and cv. In-0.
Natural variant951S → A in strain: cv. Bl-1 and cv. In-0.
Natural variant991V → I in strain: cv. Bus-1 and cv. Kas-1.
Natural variant1051K → E in strain: cv. Hiroshima.
Natural variant1141M → K in strain: cv. Bl-1 and cv. In-0.
Natural variant1191E → D in strain: cv. Nok-4.
Natural variant1981N → S in strain: cv. Bus-1.
Natural variant2951M → T in strain: cv. Cvi-0.
Natural variant3551I → T in strain: cv. WS-0.
Natural variant4251Missing in strain: cv. Cvi-0.
Natural variant4311L → F in strain: cv. Pog-0.
Natural variant4321M → I in strain: cv. In-0.
Natural variant5281H → Q in strain: cv. Ost-0.

Experimental info

Sequence conflict2161F → S in AAK50107. Ref.6
Sequence conflict2161F → S in AAM16223. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P34795 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: C4F5C7D8FE5F193B

FASTA56061,718
        10         20         30         40         50         60 
MASSTALICD TEAWKDLKGH VEDIKKTHLR DLMSDANRCQ SMMMEFDGLL LDYSRQRATV 

        70         80         90        100        110        120 
ETMDKLLNLA KASQLTEKIS RMFNGEHINS TENRSVLHVA LRAPKDAVIK ADGMNVVPEV 

       130        140        150        160        170        180 
WNVLDKIKEF SDKIRSGSWV GATGKPLKDV IAIGIGGSFL GPLFVHTALQ TDPEALESAK 

       190        200        210        220        230        240 
GRQLRFLANI DPVDVARNIS GLNPETTLVV VVSKTFTTAE TMLNARTLRE WITAALGASA 

       250        260        270        280        290        300 
VAKHMVAVST NLALVEKFGI DPNNAFAFWD WVGGRYSVCS AVGVLPLSLQ YGFSMVEKFL 

       310        320        330        340        350        360 
KGASSIDQHF QSTPFEKNIP VLLGLLSVWN VSFLGYPARA ILPYSQALEK FAPHIQQVSM 

       370        380        390        400        410        420 
ESNGKGVSID GLPLPFETGE IDFGEPGTNG QHSFYQLIHQ GRVIPCDFIG IVKSQQPVYL 

       430        440        450        460        470        480 
KGEVVSNHDE LMSNFFAQPD ALAYGKTPEQ LQKENVSENL IPHKTFSGNR PSLSLLLPEL 

       490        500        510        520        530        540 
TAYNVGQLLA IYEHRVAVQG FVWGINSFDQ WGVELGKVLA TQVRKQLHSS RTQGTAPEGF 

       550        560 
NYSTTTLLKR YLETSSEPQM 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of duplicate cytosolic phosphoglucose isomerase genes in Clarkia and comparison to the single gene in Arabidopsis."
Thomas B.R., Ford V.S., Pichersky E., Gottlieb L.D.
Genetics 135:895-905(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"The 5' leader of plant PgiC has an intron: the leader shows both the loss and maintenance of constraints compared with introns and exons in the coding region."
Gottlieb L.D., Ford V.S.
Mol. Biol. Evol. 19:1613-1623(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"DNA polymorphism in active gene and pseudogene of the cytosolic phosphoglucose isomerase (PgiC) loci in Arabidopsis halleri ssp. gemmifera."
Kawabe A., Miyashita N.T.
Mol. Biol. Evol. 20:1043-1050(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Many cultivars.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis."
Quettier A.-L., Shaw E., Eastmond P.J.
Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69195 Genomic DNA. Translation: CAA48940.1.
AJ419524 mRNA. Translation: CAD11677.1.
AB044948 Genomic DNA. Translation: BAB17635.1.
AB044949 Genomic DNA. Translation: BAB17636.1.
AB044950 Genomic DNA. Translation: BAB17637.1.
AB044951 Genomic DNA. Translation: BAB17638.1.
AB044952 Genomic DNA. Translation: BAB17639.1.
AB044953 Genomic DNA. Translation: BAB17640.1.
AB044954 Genomic DNA. Translation: BAB17641.1.
AB044955 Genomic DNA. Translation: BAB17642.1.
AB044956 Genomic DNA. Translation: BAB17643.1.
AB044957 Genomic DNA. Translation: BAB17644.1.
AB044958 Genomic DNA. Translation: BAB17645.1.
AB044959 Genomic DNA. Translation: BAB17646.1.
AB044960 Genomic DNA. Translation: BAB17647.1.
AB044961 Genomic DNA. Translation: BAB17648.1.
AB044962 Genomic DNA. Translation: BAB17649.1.
AB044963 Genomic DNA. Translation: BAB17650.1.
AB044964 Genomic DNA. Translation: BAB17651.1.
AB044965 Genomic DNA. Translation: BAB17652.1.
AB044966 Genomic DNA. Translation: BAB17653.1.
AB044967 Genomic DNA. Translation: BAB17654.1.
AB007647 Genomic DNA. Translation: BAB10630.1.
CP002688 Genomic DNA. Translation: AED94855.1.
AF372970 mRNA. Translation: AAK50107.1.
AY093962 mRNA. Translation: AAM16223.1.
PIRS41808.
RefSeqNP_199088.1. NM_123638.4.
UniGeneAt.20998.

3D structure databases

ProteinModelPortalP34795.
SMRP34795. Positions 22-555.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19533. 2 interactions.

Proteomic databases

PaxDbP34795.
PRIDEP34795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G42740.1; AT5G42740.1; AT5G42740.
GeneID834283.
KEGGath:AT5G42740.

Organism-specific databases

TAIRAT5G42740.

Phylogenomic databases

eggNOGCOG0166.
InParanoidP34795.
KOK01810.
OMARAHYVSN.
PhylomeDBP34795.

Enzyme and pathway databases

BioCycARA:AT5G42740-MONOMER.
UniPathwayUPA00109; UER00181.

Gene expression databases

ArrayExpressP34795.
GenevestigatorP34795.

Family and domain databases

Gene3D1.10.1390.10. 1 hit.
HAMAPMF_00473. G6P_isomerase.
InterProIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERPTHR11469. PTHR11469. 1 hit.
PfamPF00342. PGI. 1 hit.
[Graphical view]
PRINTSPR00662. G6PISOMERASE.
PROSITEPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP34795.

Entry information

Entry nameG6PI_ARATH
AccessionPrimary (citable) accession number: P34795
Secondary accession number(s): Q546J1 expand/collapse secondary AC list , Q94JT1, Q9FE90, Q9FXM6, Q9FXM7, Q9FXM8, Q9FXM9, Q9FXN0, Q9FXN1, Q9FXN2, Q9FXN3, Q9FXN4, Q9FXN5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names