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Protein

Glucose-6-phosphate isomerase, cytosolic

Gene

PGIC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by glycerol-3-P (G3P).1 Publication

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC), Glucose-6-phosphate isomerase, cytosolic (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase 3 (PFK3), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), ATP-dependent 6-phosphofructokinase 2 (PFK2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 7 (PFK7)
  4. Fructose-bisphosphate aldolase (M3E9.40), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (F8J2_100), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei361 – 3611Proton donorBy similarity
Active sitei392 – 3921By similarity
Active sitei517 – 5171By similarity

GO - Molecular functioni

GO - Biological processi

  • defense response to fungus, incompatible interaction Source: TAIR
  • gluconeogenesis Source: UniProtKB-KW
  • glycolytic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciARA:AT5G42740-MONOMER.
BRENDAi5.3.1.9. 399.
ReactomeiR-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase, cytosolic (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:PGIC
Ordered Locus Names:At5g42740
ORF Names:MJB21.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42740.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 560559Glucose-6-phosphate isomerase, cytosolicPRO_0000180550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP34795.
PRIDEiP34795.

Expressioni

Gene expression databases

GenevisibleiP34795. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi19533. 2 interactions.
STRINGi3702.AT5G42740.1.

Structurei

3D structure databases

ProteinModelPortaliP34795.
SMRiP34795. Positions 22-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiKOG2446. Eukaryota.
COG0166. LUCA.
InParanoidiP34795.
KOiK01810.
OMAiSHLIAPY.
OrthoDBiEOG093606CP.
PhylomeDBiP34795.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSTALICD TEAWKDLKGH VEDIKKTHLR DLMSDANRCQ SMMMEFDGLL
60 70 80 90 100
LDYSRQRATV ETMDKLLNLA KASQLTEKIS RMFNGEHINS TENRSVLHVA
110 120 130 140 150
LRAPKDAVIK ADGMNVVPEV WNVLDKIKEF SDKIRSGSWV GATGKPLKDV
160 170 180 190 200
IAIGIGGSFL GPLFVHTALQ TDPEALESAK GRQLRFLANI DPVDVARNIS
210 220 230 240 250
GLNPETTLVV VVSKTFTTAE TMLNARTLRE WITAALGASA VAKHMVAVST
260 270 280 290 300
NLALVEKFGI DPNNAFAFWD WVGGRYSVCS AVGVLPLSLQ YGFSMVEKFL
310 320 330 340 350
KGASSIDQHF QSTPFEKNIP VLLGLLSVWN VSFLGYPARA ILPYSQALEK
360 370 380 390 400
FAPHIQQVSM ESNGKGVSID GLPLPFETGE IDFGEPGTNG QHSFYQLIHQ
410 420 430 440 450
GRVIPCDFIG IVKSQQPVYL KGEVVSNHDE LMSNFFAQPD ALAYGKTPEQ
460 470 480 490 500
LQKENVSENL IPHKTFSGNR PSLSLLLPEL TAYNVGQLLA IYEHRVAVQG
510 520 530 540 550
FVWGINSFDQ WGVELGKVLA TQVRKQLHSS RTQGTAPEGF NYSTTTLLKR
560
YLETSSEPQM
Length:560
Mass (Da):61,718
Last modified:February 1, 1994 - v1
Checksum:iC4F5C7D8FE5F193B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161F → S in AAK50107 (PubMed:14593172).Curated
Sequence conflicti216 – 2161F → S in AAM16223 (PubMed:14593172).Curated

Polymorphismi

Was sequenced in many cultivars; Ag-0, Bl-1, Bus-1, Ci-0, Cvi-0, Dra-0, Edi-0, Hau-0, Hiroshima, In-0, Ita-0, Kas-1, Mr-0, Nok-4, Ost-0, Pog-0, Rou-0, Su-0, Ts-1 and Ws-0.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31S → P in strain: cv. Bus-1.
Natural varianti5 – 51T → P in strain: cv. Ag-0, cv. Bus-1, cv. Dra-0, cv. Hiroshima and cv. Kas-1.
Natural varianti36 – 361A → V in strain: cv. Bus-1.
Natural varianti73 – 731S → A in strain: cv. Bl-1 and cv. In-0.
Natural varianti95 – 951S → A in strain: cv. Bl-1 and cv. In-0.
Natural varianti99 – 991V → I in strain: cv. Bus-1 and cv. Kas-1.
Natural varianti105 – 1051K → E in strain: cv. Hiroshima.
Natural varianti114 – 1141M → K in strain: cv. Bl-1 and cv. In-0.
Natural varianti119 – 1191E → D in strain: cv. Nok-4.
Natural varianti198 – 1981N → S in strain: cv. Bus-1.
Natural varianti295 – 2951M → T in strain: cv. Cvi-0.
Natural varianti355 – 3551I → T in strain: cv. WS-0.
Natural varianti425 – 4251Missing in strain: cv. Cvi-0.
Natural varianti431 – 4311L → F in strain: cv. Pog-0.
Natural varianti432 – 4321M → I in strain: cv. In-0.
Natural varianti528 – 5281H → Q in strain: cv. Ost-0.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69195 Genomic DNA. Translation: CAA48940.1.
AJ419524 mRNA. Translation: CAD11677.1.
AB044948 Genomic DNA. Translation: BAB17635.1.
AB044949 Genomic DNA. Translation: BAB17636.1.
AB044950 Genomic DNA. Translation: BAB17637.1.
AB044951 Genomic DNA. Translation: BAB17638.1.
AB044952 Genomic DNA. Translation: BAB17639.1.
AB044953 Genomic DNA. Translation: BAB17640.1.
AB044954 Genomic DNA. Translation: BAB17641.1.
AB044955 Genomic DNA. Translation: BAB17642.1.
AB044956 Genomic DNA. Translation: BAB17643.1.
AB044957 Genomic DNA. Translation: BAB17644.1.
AB044958 Genomic DNA. Translation: BAB17645.1.
AB044959 Genomic DNA. Translation: BAB17646.1.
AB044960 Genomic DNA. Translation: BAB17647.1.
AB044961 Genomic DNA. Translation: BAB17648.1.
AB044962 Genomic DNA. Translation: BAB17649.1.
AB044963 Genomic DNA. Translation: BAB17650.1.
AB044964 Genomic DNA. Translation: BAB17651.1.
AB044965 Genomic DNA. Translation: BAB17652.1.
AB044966 Genomic DNA. Translation: BAB17653.1.
AB044967 Genomic DNA. Translation: BAB17654.1.
AB007647 Genomic DNA. Translation: BAB10630.1.
CP002688 Genomic DNA. Translation: AED94855.1.
AF372970 mRNA. Translation: AAK50107.1.
AY093962 mRNA. Translation: AAM16223.1.
PIRiS41808.
RefSeqiNP_199088.1. NM_123638.4.
UniGeneiAt.20998.

Genome annotation databases

EnsemblPlantsiAT5G42740.1; AT5G42740.1; AT5G42740.
GeneIDi834283.
GrameneiAT5G42740.1; AT5G42740.1; AT5G42740.
KEGGiath:AT5G42740.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69195 Genomic DNA. Translation: CAA48940.1.
AJ419524 mRNA. Translation: CAD11677.1.
AB044948 Genomic DNA. Translation: BAB17635.1.
AB044949 Genomic DNA. Translation: BAB17636.1.
AB044950 Genomic DNA. Translation: BAB17637.1.
AB044951 Genomic DNA. Translation: BAB17638.1.
AB044952 Genomic DNA. Translation: BAB17639.1.
AB044953 Genomic DNA. Translation: BAB17640.1.
AB044954 Genomic DNA. Translation: BAB17641.1.
AB044955 Genomic DNA. Translation: BAB17642.1.
AB044956 Genomic DNA. Translation: BAB17643.1.
AB044957 Genomic DNA. Translation: BAB17644.1.
AB044958 Genomic DNA. Translation: BAB17645.1.
AB044959 Genomic DNA. Translation: BAB17646.1.
AB044960 Genomic DNA. Translation: BAB17647.1.
AB044961 Genomic DNA. Translation: BAB17648.1.
AB044962 Genomic DNA. Translation: BAB17649.1.
AB044963 Genomic DNA. Translation: BAB17650.1.
AB044964 Genomic DNA. Translation: BAB17651.1.
AB044965 Genomic DNA. Translation: BAB17652.1.
AB044966 Genomic DNA. Translation: BAB17653.1.
AB044967 Genomic DNA. Translation: BAB17654.1.
AB007647 Genomic DNA. Translation: BAB10630.1.
CP002688 Genomic DNA. Translation: AED94855.1.
AF372970 mRNA. Translation: AAK50107.1.
AY093962 mRNA. Translation: AAM16223.1.
PIRiS41808.
RefSeqiNP_199088.1. NM_123638.4.
UniGeneiAt.20998.

3D structure databases

ProteinModelPortaliP34795.
SMRiP34795. Positions 22-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19533. 2 interactions.
STRINGi3702.AT5G42740.1.

Proteomic databases

PaxDbiP34795.
PRIDEiP34795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42740.1; AT5G42740.1; AT5G42740.
GeneIDi834283.
GrameneiAT5G42740.1; AT5G42740.1; AT5G42740.
KEGGiath:AT5G42740.

Organism-specific databases

TAIRiAT5G42740.

Phylogenomic databases

eggNOGiKOG2446. Eukaryota.
COG0166. LUCA.
InParanoidiP34795.
KOiK01810.
OMAiSHLIAPY.
OrthoDBiEOG093606CP.
PhylomeDBiP34795.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
BioCyciARA:AT5G42740-MONOMER.
BRENDAi5.3.1.9. 399.
ReactomeiR-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.

Miscellaneous databases

PROiP34795.

Gene expression databases

GenevisibleiP34795. AT.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_ARATH
AccessioniPrimary (citable) accession number: P34795
Secondary accession number(s): Q546J1
, Q94JT1, Q9FE90, Q9FXM6, Q9FXM7, Q9FXM8, Q9FXM9, Q9FXN0, Q9FXN1, Q9FXN2, Q9FXN3, Q9FXN4, Q9FXN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.