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P34795

- G6PI_ARATH

UniProt

P34795 - G6PI_ARATH

Protein

Glucose-6-phosphate isomerase, cytosolic

Gene

PGIC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.

    Enzyme regulationi

    Inhibited by glycerol-3-P (G3P).1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei361 – 3611Proton donorBy similarity
    Active sitei392 – 3921By similarity
    Active sitei517 – 5171By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response to fungus, incompatible interaction Source: TAIR
    2. gluconeogenesis Source: UniProtKB-KW
    3. glycolytic process Source: UniProtKB-UniPathway
    4. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Gluconeogenesis, Glycolysis

    Enzyme and pathway databases

    BioCyciARA:AT5G42740-MONOMER.
    UniPathwayiUPA00109; UER00181.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate isomerase, cytosolic (EC:5.3.1.9)
    Short name:
    GPI
    Alternative name(s):
    Phosphoglucose isomerase
    Short name:
    PGI
    Phosphohexose isomerase
    Short name:
    PHI
    Gene namesi
    Name:PGIC
    Ordered Locus Names:At5g42740
    ORF Names:MJB21.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G42740.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 560559Glucose-6-phosphate isomerase, cytosolicPRO_0000180550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP34795.
    PRIDEiP34795.

    Expressioni

    Gene expression databases

    ArrayExpressiP34795.
    GenevestigatoriP34795.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi19533. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP34795.
    SMRiP34795. Positions 22-555.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI family.Curated

    Phylogenomic databases

    eggNOGiCOG0166.
    InParanoidiP34795.
    KOiK01810.
    OMAiRAHYVSN.
    PhylomeDBiP34795.

    Family and domain databases

    Gene3Di1.10.1390.10. 1 hit.
    HAMAPiMF_00473. G6P_isomerase.
    InterProiIPR001672. G6P_Isomerase.
    IPR023096. G6P_Isomerase_C.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view]
    PANTHERiPTHR11469. PTHR11469. 1 hit.
    PfamiPF00342. PGI. 1 hit.
    [Graphical view]
    PRINTSiPR00662. G6PISOMERASE.
    PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P34795-1 [UniParc]FASTAAdd to Basket

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    MASSTALICD TEAWKDLKGH VEDIKKTHLR DLMSDANRCQ SMMMEFDGLL    50
    LDYSRQRATV ETMDKLLNLA KASQLTEKIS RMFNGEHINS TENRSVLHVA 100
    LRAPKDAVIK ADGMNVVPEV WNVLDKIKEF SDKIRSGSWV GATGKPLKDV 150
    IAIGIGGSFL GPLFVHTALQ TDPEALESAK GRQLRFLANI DPVDVARNIS 200
    GLNPETTLVV VVSKTFTTAE TMLNARTLRE WITAALGASA VAKHMVAVST 250
    NLALVEKFGI DPNNAFAFWD WVGGRYSVCS AVGVLPLSLQ YGFSMVEKFL 300
    KGASSIDQHF QSTPFEKNIP VLLGLLSVWN VSFLGYPARA ILPYSQALEK 350
    FAPHIQQVSM ESNGKGVSID GLPLPFETGE IDFGEPGTNG QHSFYQLIHQ 400
    GRVIPCDFIG IVKSQQPVYL KGEVVSNHDE LMSNFFAQPD ALAYGKTPEQ 450
    LQKENVSENL IPHKTFSGNR PSLSLLLPEL TAYNVGQLLA IYEHRVAVQG 500
    FVWGINSFDQ WGVELGKVLA TQVRKQLHSS RTQGTAPEGF NYSTTTLLKR 550
    YLETSSEPQM 560
    Length:560
    Mass (Da):61,718
    Last modified:February 1, 1994 - v1
    Checksum:iC4F5C7D8FE5F193B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti216 – 2161F → S in AAK50107. (PubMed:14593172)Curated
    Sequence conflicti216 – 2161F → S in AAM16223. (PubMed:14593172)Curated

    Polymorphismi

    Was sequenced in many cultivars; Ag-0, Bl-1, Bus-1, Ci-0, Cvi-0, Dra-0, Edi-0, Hau-0, Hiroshima, In-0, Ita-0, Kas-1, Mr-0, Nok-4, Ost-0, Pog-0, Rou-0, Su-0, Ts-1 and Ws-0.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti3 – 31S → P in strain: cv. Bus-1.
    Natural varianti5 – 51T → P in strain: cv. Ag-0, cv. Bus-1, cv. Dra-0, cv. Hiroshima and cv. Kas-1.
    Natural varianti36 – 361A → V in strain: cv. Bus-1.
    Natural varianti73 – 731S → A in strain: cv. Bl-1 and cv. In-0.
    Natural varianti95 – 951S → A in strain: cv. Bl-1 and cv. In-0.
    Natural varianti99 – 991V → I in strain: cv. Bus-1 and cv. Kas-1.
    Natural varianti105 – 1051K → E in strain: cv. Hiroshima.
    Natural varianti114 – 1141M → K in strain: cv. Bl-1 and cv. In-0.
    Natural varianti119 – 1191E → D in strain: cv. Nok-4.
    Natural varianti198 – 1981N → S in strain: cv. Bus-1.
    Natural varianti295 – 2951M → T in strain: cv. Cvi-0.
    Natural varianti355 – 3551I → T in strain: cv. WS-0.
    Natural varianti425 – 4251Missing in strain: cv. Cvi-0.
    Natural varianti431 – 4311L → F in strain: cv. Pog-0.
    Natural varianti432 – 4321M → I in strain: cv. In-0.
    Natural varianti528 – 5281H → Q in strain: cv. Ost-0.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69195 Genomic DNA. Translation: CAA48940.1.
    AJ419524 mRNA. Translation: CAD11677.1.
    AB044948 Genomic DNA. Translation: BAB17635.1.
    AB044949 Genomic DNA. Translation: BAB17636.1.
    AB044950 Genomic DNA. Translation: BAB17637.1.
    AB044951 Genomic DNA. Translation: BAB17638.1.
    AB044952 Genomic DNA. Translation: BAB17639.1.
    AB044953 Genomic DNA. Translation: BAB17640.1.
    AB044954 Genomic DNA. Translation: BAB17641.1.
    AB044955 Genomic DNA. Translation: BAB17642.1.
    AB044956 Genomic DNA. Translation: BAB17643.1.
    AB044957 Genomic DNA. Translation: BAB17644.1.
    AB044958 Genomic DNA. Translation: BAB17645.1.
    AB044959 Genomic DNA. Translation: BAB17646.1.
    AB044960 Genomic DNA. Translation: BAB17647.1.
    AB044961 Genomic DNA. Translation: BAB17648.1.
    AB044962 Genomic DNA. Translation: BAB17649.1.
    AB044963 Genomic DNA. Translation: BAB17650.1.
    AB044964 Genomic DNA. Translation: BAB17651.1.
    AB044965 Genomic DNA. Translation: BAB17652.1.
    AB044966 Genomic DNA. Translation: BAB17653.1.
    AB044967 Genomic DNA. Translation: BAB17654.1.
    AB007647 Genomic DNA. Translation: BAB10630.1.
    CP002688 Genomic DNA. Translation: AED94855.1.
    AF372970 mRNA. Translation: AAK50107.1.
    AY093962 mRNA. Translation: AAM16223.1.
    PIRiS41808.
    RefSeqiNP_199088.1. NM_123638.4.
    UniGeneiAt.20998.

    Genome annotation databases

    EnsemblPlantsiAT5G42740.1; AT5G42740.1; AT5G42740.
    GeneIDi834283.
    KEGGiath:AT5G42740.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69195 Genomic DNA. Translation: CAA48940.1 .
    AJ419524 mRNA. Translation: CAD11677.1 .
    AB044948 Genomic DNA. Translation: BAB17635.1 .
    AB044949 Genomic DNA. Translation: BAB17636.1 .
    AB044950 Genomic DNA. Translation: BAB17637.1 .
    AB044951 Genomic DNA. Translation: BAB17638.1 .
    AB044952 Genomic DNA. Translation: BAB17639.1 .
    AB044953 Genomic DNA. Translation: BAB17640.1 .
    AB044954 Genomic DNA. Translation: BAB17641.1 .
    AB044955 Genomic DNA. Translation: BAB17642.1 .
    AB044956 Genomic DNA. Translation: BAB17643.1 .
    AB044957 Genomic DNA. Translation: BAB17644.1 .
    AB044958 Genomic DNA. Translation: BAB17645.1 .
    AB044959 Genomic DNA. Translation: BAB17646.1 .
    AB044960 Genomic DNA. Translation: BAB17647.1 .
    AB044961 Genomic DNA. Translation: BAB17648.1 .
    AB044962 Genomic DNA. Translation: BAB17649.1 .
    AB044963 Genomic DNA. Translation: BAB17650.1 .
    AB044964 Genomic DNA. Translation: BAB17651.1 .
    AB044965 Genomic DNA. Translation: BAB17652.1 .
    AB044966 Genomic DNA. Translation: BAB17653.1 .
    AB044967 Genomic DNA. Translation: BAB17654.1 .
    AB007647 Genomic DNA. Translation: BAB10630.1 .
    CP002688 Genomic DNA. Translation: AED94855.1 .
    AF372970 mRNA. Translation: AAK50107.1 .
    AY093962 mRNA. Translation: AAM16223.1 .
    PIRi S41808.
    RefSeqi NP_199088.1. NM_123638.4.
    UniGenei At.20998.

    3D structure databases

    ProteinModelPortali P34795.
    SMRi P34795. Positions 22-555.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19533. 2 interactions.

    Proteomic databases

    PaxDbi P34795.
    PRIDEi P34795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G42740.1 ; AT5G42740.1 ; AT5G42740 .
    GeneIDi 834283.
    KEGGi ath:AT5G42740.

    Organism-specific databases

    TAIRi AT5G42740.

    Phylogenomic databases

    eggNOGi COG0166.
    InParanoidi P34795.
    KOi K01810.
    OMAi RAHYVSN.
    PhylomeDBi P34795.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00181 .
    BioCyci ARA:AT5G42740-MONOMER.

    Miscellaneous databases

    PROi P34795.

    Gene expression databases

    ArrayExpressi P34795.
    Genevestigatori P34795.

    Family and domain databases

    Gene3Di 1.10.1390.10. 1 hit.
    HAMAPi MF_00473. G6P_isomerase.
    InterProi IPR001672. G6P_Isomerase.
    IPR023096. G6P_Isomerase_C.
    IPR018189. Phosphoglucose_isomerase_CS.
    [Graphical view ]
    PANTHERi PTHR11469. PTHR11469. 1 hit.
    Pfami PF00342. PGI. 1 hit.
    [Graphical view ]
    PRINTSi PR00662. G6PISOMERASE.
    PROSITEi PS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
    PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
    PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of duplicate cytosolic phosphoglucose isomerase genes in Clarkia and comparison to the single gene in Arabidopsis."
      Thomas B.R., Ford V.S., Pichersky E., Gottlieb L.D.
      Genetics 135:895-905(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "The 5' leader of plant PgiC has an intron: the leader shows both the loss and maintenance of constraints compared with introns and exons in the coding region."
      Gottlieb L.D., Ford V.S.
      Mol. Biol. Evol. 19:1613-1623(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. "DNA polymorphism in active gene and pseudogene of the cytosolic phosphoglucose isomerase (PgiC) loci in Arabidopsis halleri ssp. gemmifera."
      Kawabe A., Miyashita N.T.
      Mol. Biol. Evol. 20:1043-1050(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Many cultivars.
    4. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
      Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "SUGAR-DEPENDENT6 encodes a mitochondrial flavin adenine dinucleotide-dependent glycerol-3-p dehydrogenase, which is required for glycerol catabolism and post germinative seedling growth in Arabidopsis."
      Quettier A.-L., Shaw E., Eastmond P.J.
      Plant Physiol. 148:519-528(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiG6PI_ARATH
    AccessioniPrimary (citable) accession number: P34795
    Secondary accession number(s): Q546J1
    , Q94JT1, Q9FE90, Q9FXM6, Q9FXM7, Q9FXM8, Q9FXM9, Q9FXN0, Q9FXN1, Q9FXN2, Q9FXN3, Q9FXN4, Q9FXN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3