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Protein

Peptidyl-prolyl cis-trans isomerase CYP18-3

Gene

CYP18-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in de-etiolation. Reduces the sensitivity to brassinosteroids by decreasing somehow the abundance of the partially dephosphorylated form of BES1. Triggers the activation of bacterial AvrRpt2 protease activity upon infection by P.syringae. Activated AvrRpt2 confers virulence in plant lacking the RPS2 resistance gene. In plants expressing RPS2, the AvrRpt2-mediated degradation of RIN4 activates RPS2, which induces hypersensitive response (HR) and plant resistance.4 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulationi

Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase.

GO - Molecular functioni

  • peptide binding Source: UniProtKB-KW
  • peptidyl-prolyl cis-trans isomerase activity Source: TAIR

GO - Biological processi

  • blue light signaling pathway Source: TAIR
  • brassinosteroid mediated signaling pathway Source: TAIR
  • de-etiolation Source: TAIR
  • plant-type hypersensitive response Source: UniProtKB-KW
  • protein folding Source: InterPro
  • red, far-red light phototransduction Source: TAIR
  • regulation of protein phosphorylation Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cytokinin Source: TAIR
  • response to light stimulus Source: TAIR
  • signal transduction Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase, Rotamase

Keywords - Biological processi

Hypersensitive response, Plant defense

Keywords - Ligandi

Cyclosporin

Enzyme and pathway databases

BRENDAi5.2.1.8. 399.
ReactomeiR-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase CYP18-3 (EC:5.2.1.8)
Short name:
PPIase CYP18-3
Alternative name(s):
Cyclophilin of 18 kDa 3
Cyclosporin A-binding protein
Rotamase cyclophilin-1
Gene namesi
Name:CYP18-3
Synonyms:ROC1
Ordered Locus Names:At4g38740
ORF Names:T9A14.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G38740.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62R → A: Loss of PPIase activity. 1 Publication1
Mutagenesisi128W → A: Loss of PPIase activity. 1 Publication1
Mutagenesisi133H → A: Decreased PPIase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000641331 – 172Peptidyl-prolyl cis-trans isomerase CYP18-3Add BLAST172

Proteomic databases

PaxDbiP34790.
PRIDEiP34790.

PTM databases

iPTMnetiP34790.

Expressioni

Tissue specificityi

Pollen (at protein level). Ubiquitous.4 Publications

Inductioni

Up-regulated by light, salt and wounding. Down-regulated by cytokinin treatment.3 Publications

Gene expression databases

GenevisibleiP34790. AT.

Interactioni

Subunit structurei

Interacts with P.syringae AvrRpt2 and with A.tumefaciens VirD2, but not with BES1.4 Publications

Protein-protein interaction databases

BioGridi15309. 6 interactors.
IntActiP34790. 2 interactors.
MINTiMINT-8061878.
STRINGi3702.AT4G38740.1.

Structurei

3D structure databases

ProteinModelPortaliP34790.
SMRiP34790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 170PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST164

Sequence similaritiesi

Belongs to the cyclophilin-type PPIase family.Curated
Contains 1 PPIase cyclophilin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0865. Eukaryota.
COG0652. LUCA.
HOGENOMiHOG000065981.
InParanoidiP34790.
KOiK01802.
OMAiHSIYNND.
OrthoDBiEOG09360PV0.
PhylomeDBiP34790.

Family and domain databases

Gene3Di2.40.100.10. 1 hit.
InterProiIPR029000. Cyclophilin-like_dom.
IPR024936. Cyclophilin-type_PPIase.
IPR020892. Cyclophilin-type_PPIase_CS.
IPR002130. Cyclophilin-type_PPIase_dom.
[Graphical view]
PANTHERiPTHR11071. PTHR11071. 1 hit.
PfamiPF00160. Pro_isomerase. 1 hit.
[Graphical view]
PIRSFiPIRSF001467. Peptidylpro_ismrse. 1 hit.
PRINTSiPR00153. CSAPPISMRASE.
SUPFAMiSSF50891. SSF50891. 1 hit.
PROSITEiPS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPKVYFDM TIDGQPAGRI VMELYTDKTP RTAENFRALC TGEKGVGGTG
60 70 80 90 100
KPLHFKGSKF HRVIPNFMCQ GGDFTAGNGT GGESIYGSKF EDENFERKHT
110 120 130 140 150
GPGILSMANA GANTNGSQFF ICTVKTDWLD GKHVVFGQVV EGLDVVKAIE
160 170
KVGSSSGKPT KPVVVADCGQ LS
Length:172
Mass (Da):18,373
Last modified:February 1, 1994 - v1
Checksum:iB49B8C68E60E15E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14844 mRNA. Translation: AAA20047.1.
AL035656 Genomic DNA. Translation: CAB38608.1.
AL161593 Genomic DNA. Translation: CAB80537.1.
CP002687 Genomic DNA. Translation: AEE86970.1.
AY093227 mRNA. Translation: AAM13226.1.
BT003397 mRNA. Translation: AAO30060.1.
AK226392 mRNA. Translation: BAE98538.1.
AY088103 mRNA. Translation: AAM65649.1.
PIRiT06073.
RefSeqiNP_195585.1. NM_120034.2.
UniGeneiAt.24097.
At.24759.
At.71539.
At.72496.

Genome annotation databases

EnsemblPlantsiAT4G38740.1; AT4G38740.1; AT4G38740.
GeneIDi830029.
GrameneiAT4G38740.1; AT4G38740.1; AT4G38740.
KEGGiath:AT4G38740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14844 mRNA. Translation: AAA20047.1.
AL035656 Genomic DNA. Translation: CAB38608.1.
AL161593 Genomic DNA. Translation: CAB80537.1.
CP002687 Genomic DNA. Translation: AEE86970.1.
AY093227 mRNA. Translation: AAM13226.1.
BT003397 mRNA. Translation: AAO30060.1.
AK226392 mRNA. Translation: BAE98538.1.
AY088103 mRNA. Translation: AAM65649.1.
PIRiT06073.
RefSeqiNP_195585.1. NM_120034.2.
UniGeneiAt.24097.
At.24759.
At.71539.
At.72496.

3D structure databases

ProteinModelPortaliP34790.
SMRiP34790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15309. 6 interactors.
IntActiP34790. 2 interactors.
MINTiMINT-8061878.
STRINGi3702.AT4G38740.1.

PTM databases

iPTMnetiP34790.

Proteomic databases

PaxDbiP34790.
PRIDEiP34790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G38740.1; AT4G38740.1; AT4G38740.
GeneIDi830029.
GrameneiAT4G38740.1; AT4G38740.1; AT4G38740.
KEGGiath:AT4G38740.

Organism-specific databases

TAIRiAT4G38740.

Phylogenomic databases

eggNOGiKOG0865. Eukaryota.
COG0652. LUCA.
HOGENOMiHOG000065981.
InParanoidiP34790.
KOiK01802.
OMAiHSIYNND.
OrthoDBiEOG09360PV0.
PhylomeDBiP34790.

Enzyme and pathway databases

BRENDAi5.2.1.8. 399.
ReactomeiR-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiP34790.

Gene expression databases

GenevisibleiP34790. AT.

Family and domain databases

Gene3Di2.40.100.10. 1 hit.
InterProiIPR029000. Cyclophilin-like_dom.
IPR024936. Cyclophilin-type_PPIase.
IPR020892. Cyclophilin-type_PPIase_CS.
IPR002130. Cyclophilin-type_PPIase_dom.
[Graphical view]
PANTHERiPTHR11071. PTHR11071. 1 hit.
PfamiPF00160. Pro_isomerase. 1 hit.
[Graphical view]
PIRSFiPIRSF001467. Peptidylpro_ismrse. 1 hit.
PRINTSiPR00153. CSAPPISMRASE.
SUPFAMiSSF50891. SSF50891. 1 hit.
PROSITEiPS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP18C_ARATH
AccessioniPrimary (citable) accession number: P34790
Secondary accession number(s): Q0WWG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.