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Protein

Protein WHI3

Gene

WHI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in size control and cell cycle.1 Publication

GO - Molecular functioni

  • mRNA binding Source: SGD
  • nucleotide binding Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell wall organization or biogenesis Source: CACAO
  • cytoplasmic sequestering of protein Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • mRNA destabilization Source: SGD
  • positive regulation of cell size Source: CACAO
  • positive regulation of cell-substrate adhesion Source: CACAO
  • positive regulation of response to extracellular stimulus Source: CACAO
  • positive regulation of transcription, DNA-templated Source: CACAO
  • positive regulation of translation Source: CACAO
  • positive regulation of translational elongation Source: CACAO
  • pseudohyphal growth Source: SGD
  • regulation of cell size Source: SGD
  • regulation of mRNA catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33207-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein WHI3
Gene namesi
Name:WHI3
Ordered Locus Names:YNL197C
ORF Names:N1382
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL197C.
SGDiS000005141. WHI3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661Protein WHI3PRO_0000082005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei231 – 2311PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP34761.
PeptideAtlasiP34761.
TopDownProteomicsiP34761.

PTM databases

iPTMnetiP34761.

Interactioni

Protein-protein interaction databases

BioGridi35636. 507 interactions.
IntActiP34761. 16 interactions.
MINTiMINT-4500279.

Structurei

3D structure databases

ProteinModelPortaliP34761.
SMRiP34761. Positions 536-625.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini538 – 62588RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi247 – 27731Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000067542.
HOGENOMiHOG000074699.
InParanoidiP34761.
OMAiVRGPNSR.
OrthoDBiEOG7PP5GG.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSVYFDQT GSFASSSDNV VSSTTNTHNI SPSHRSSLNL NTTSHPHEAS
60 70 80 90 100
GRGSASGELY LNDTNSPLAI SSMLNTLALG SMPQDIASSN ISNHDNNIKG
110 120 130 140 150
SYSLKLSNVA KDITLRECYA IFALAEGVKS IELQKKNSSS SITSASLEDE
160 170 180 190 200
NDIFIIARFE LLNLAINYAV ILNSKNELFG PSFPNKTTVE IIDDTTKNLV
210 220 230 240 250
SFPSSAIFND TSRLNKSNSG MKRPSLLSQR SRFSFSDPFS NDSPLSQQQS
260 270 280 290 300
QQQQQQPQQP QQHSTQKHSP QQCNQQQVNS SIPLSSQGQV IGLHSNHSHQ
310 320 330 340 350
DLSVESTIQT SDIGKSFLLR DNTEINEKIW GTSGIPSSIN GYMSTPQPST
360 370 380 390 400
PTLEWGNTSA SQHGSSFFLP SAASTAIAPT NSNTSANANA SSNNGASNNG
410 420 430 440 450
ANQALSASSQ QPMMQIGNTI NTSLTSSNSL PPYGLMSSQS QHISNMVNTS
460 470 480 490 500
DMNITPQKQN RFMQQPQPEH MYPVNQSNTP QKVPPARLSS SRNSHKNNST
510 520 530 540 550
TSLSSNITGS ASISQADLSL LARIPPPANP ADQNPPCNTL YVGNLPSDAT
560 570 580 590 600
EQELRQLFSG QEGFRRLSFR NKNTTSNGHS HGPMCFVEFD DVSFATRALA
610 620 630 640 650
ELYGRQLPRS TVSSKGGIRL SFSKNPLGVR GPNSRRGGSG NPNPNVNMLS
660
SYNSNVGHIK N
Length:661
Mass (Da):71,253
Last modified:February 1, 1994 - v1
Checksum:i47A6B9716CF96043
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01095 Genomic DNA. Translation: AAA03320.1.
X78898 Genomic DNA. Translation: CAA55511.1.
Z71473 Genomic DNA. Translation: CAA96092.1.
BK006947 Genomic DNA. Translation: DAA10356.1.
PIRiS50734.
RefSeqiNP_014202.1. NM_001183035.1.

Genome annotation databases

EnsemblFungiiYNL197C; YNL197C; YNL197C.
GeneIDi855524.
KEGGisce:YNL197C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01095 Genomic DNA. Translation: AAA03320.1.
X78898 Genomic DNA. Translation: CAA55511.1.
Z71473 Genomic DNA. Translation: CAA96092.1.
BK006947 Genomic DNA. Translation: DAA10356.1.
PIRiS50734.
RefSeqiNP_014202.1. NM_001183035.1.

3D structure databases

ProteinModelPortaliP34761.
SMRiP34761. Positions 536-625.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35636. 507 interactions.
IntActiP34761. 16 interactions.
MINTiMINT-4500279.

PTM databases

iPTMnetiP34761.

Proteomic databases

MaxQBiP34761.
PeptideAtlasiP34761.
TopDownProteomicsiP34761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL197C; YNL197C; YNL197C.
GeneIDi855524.
KEGGisce:YNL197C.

Organism-specific databases

EuPathDBiFungiDB:YNL197C.
SGDiS000005141. WHI3.

Phylogenomic databases

GeneTreeiENSGT00530000067542.
HOGENOMiHOG000074699.
InParanoidiP34761.
OMAiVRGPNSR.
OrthoDBiEOG7PP5GG.

Enzyme and pathway databases

BioCyciYEAST:G3O-33207-MONOMER.

Miscellaneous databases

NextBioi979559.
PROiP34761.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of WHI3, a size-control gene of Saccharomyces cerevisiae."
    Nash R.S., Volpe T., Futcher B.
    Genetics 157:1469-1480(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function."
    Jonniaux J.-L., Coster F., Purnelle B., Goffeau A.
    Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWHI3_YEAST
AccessioniPrimary (citable) accession number: P34761
Secondary accession number(s): D6W0Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5730 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.