P34761 (WHI3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein WHI3

Gene names

Name:	WHI3
Ordered Locus Names:	YNL197C
ORF Names:	N1382

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in size control and cell cycle. Ref.1
Miscellaneous	Present with 5730 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Cell cycle
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic sequestering of protein Inferred from mutant phenotype. Source: SGD invasive growth in response to glucose limitation Inferred from mutant phenotype. Source: SGD pseudohyphal growth Inferred from mutant phenotype. Source: SGD regulation of cell size Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from mutant phenotype. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	Protein WHI3		PRO_0000082005
Regions
	Domain	538 – 625	88	RRM
	Compositional bias	247 – 277	31	Gln-rich
Amino acid modifications
	Modified residue	298	1	Phosphoserine Ref.7
	Modified residue	491	1	Phosphoserine Ref.6
	Modified residue	494	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P34761 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 47A6B9716CF96043	FASTA	661	71,253
	10 20 30 40 50 60 MQSSVYFDQT GSFASSSDNV VSSTTNTHNI SPSHRSSLNL NTTSHPHEAS GRGSASGELY 70 80 90 100 110 120 LNDTNSPLAI SSMLNTLALG SMPQDIASSN ISNHDNNIKG SYSLKLSNVA KDITLRECYA 130 140 150 160 170 180 IFALAEGVKS IELQKKNSSS SITSASLEDE NDIFIIARFE LLNLAINYAV ILNSKNELFG 190 200 210 220 230 240 PSFPNKTTVE IIDDTTKNLV SFPSSAIFND TSRLNKSNSG MKRPSLLSQR SRFSFSDPFS 250 260 270 280 290 300 NDSPLSQQQS QQQQQQPQQP QQHSTQKHSP QQCNQQQVNS SIPLSSQGQV IGLHSNHSHQ 310 320 330 340 350 360 DLSVESTIQT SDIGKSFLLR DNTEINEKIW GTSGIPSSIN GYMSTPQPST PTLEWGNTSA 370 380 390 400 410 420 SQHGSSFFLP SAASTAIAPT NSNTSANANA SSNNGASNNG ANQALSASSQ QPMMQIGNTI 430 440 450 460 470 480 NTSLTSSNSL PPYGLMSSQS QHISNMVNTS DMNITPQKQN RFMQQPQPEH MYPVNQSNTP 490 500 510 520 530 540 QKVPPARLSS SRNSHKNNST TSLSSNITGS ASISQADLSL LARIPPPANP ADQNPPCNTL 550 560 570 580 590 600 YVGNLPSDAT EQELRQLFSG QEGFRRLSFR NKNTTSNGHS HGPMCFVEFD DVSFATRALA 610 620 630 640 650 660 ELYGRQLPRS TVSSKGGIRL SFSKNPLGVR GPNSRRGGSG NPNPNVNMLS SYNSNVGHIK N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of WHI3, a size-control gene of Saccharomyces cerevisiae." Nash R.S., Volpe T., Futcher B. Genetics 157:1469-1480(2001) [PubMed: 11290704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function." Jonniaux J.-L., Coster F., Purnelle B., Goffeau A. Yeast 10:1639-1645(1994) [PubMed: 7725799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491 AND SER-494, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01095 Genomic DNA. Translation: AAA03320.1. X78898 Genomic DNA. Translation: CAA55511.1. Z71473 Genomic DNA. Translation: CAA96092.1. BK006947 Genomic DNA. Translation: DAA10356.1.
PIR	S50734.
RefSeq	NP_014202.1. NM_001183035.1.
3D structure databases
ProteinModelPortal	P34761.
SMR	P34761. Positions 526-624.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34761. 16 interactions.
MINT	MINT-4500279.
STRING	P34761.
Proteomic databases
PeptideAtlas	P34761.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL197C; YNL197C; YNL197C.
GeneID	855524.
KEGG	sce:YNL197C.
NMPDR	fig|4932.3.peg.5267.
Organism-specific databases
CYGD	YNL197c.
SGD	S000005141. WHI3.
Phylogenomic databases
eggNOG	fuNOG10848.
GeneTree	EFGT00050000001634.
HOGENOM	HBG203116.
OMA	QRSRFSF.
OrthoDB	EOG42592X.
Gene expression databases
ArrayExpress	P34761.
Genevestigator	P34761.
GermOnline	YNL197C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979559.

Entry information

Entry name

WHI3_YEAST

Accession

Primary (citable) accession number: P34761
Secondary accession number(s): D6W0Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program