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P34760 (TSA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Peroxiredoxin TSA1
EC=1.11.1.15
Alternative name(s):
Cytoplasmic thiol peroxidase 1
Short name=cTPx 1
PRP
Thiol-specific antioxidant protein 1
Thioredoxin peroxidase
Gene names
Name:TSA1
Synonyms:TPX1, TSA, ZRG14
Ordered Locus Names:YML028W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Physiologically important antioxidant which constitutes an enzymatic defense against sulfur-containing radicals. Can provide protection against a thiol-containing oxidation system but not against an oxidation system without thiol.

Catalytic activity

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Subunit structure

Homodimer; disulfide-linked, upon oxidation. Ref.6

Subcellular location

Cytoplasm.

Induction

By H2O2.

Post-translational modification

The Cys-48-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-48 (probably Cys-SOH) rapidly reacts with Cys-171-SH of the other subunit to form an intermolecular disulfide. This disulfide is subsequently reduced by thioredoxin.

Forms a transient disulfide bond with SRX1 during the reduction of cysteine sulfinic acid (-SO2H).

Miscellaneous

Present with 378000 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the AhpC/TSA family.

Contains 1 thioredoxin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 196195Peroxiredoxin TSA1
PRO_0000135095

Regions

Domain3 – 161159Thioredoxin

Sites

Active site481Cysteine sulfenic acid (-SOH) intermediate By similarity

Amino acid modifications

Modified residue1741Phosphothreonine Ref.7
Disulfide bond48Interchain (with C-171); in linked form By similarity
Disulfide bond48Interchain (with C-84 in SRX1); transient Ref.6
Disulfide bond171Interchain (with C-48); in linked form By similarity

Sequences

Sequence LengthMass (Da)Tools
P34760 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9ACF40E410F2C04A

FASTA19621,590
        10         20         30         40         50         60 
MVAQVQKQAP TFKKTAVVDG VFDEVSLDKY KGKYVVLAFI PLAFTFVCPT EIIAFSEAAK 

        70         80         90        100        110        120 
KFEEQGAQVL FASTDSEYSL LAWTNIPRKE GGLGPINIPL LADTNHSLSR DYGVLIEEEG 

       130        140        150        160        170        180 
VALRGLFIID PKGVIRHITI NDLPVGRNVD EALRLVEAFQ WTDKNGTVLP CNWTPGAATI 

       190 
KPTVEDSKEY FEAANK

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae."
Chae H.Z., Kim I.-H., Kim K., Rhee S.G.
J. Biol. Chem. 268:16815-16821(1993) [PubMed: 8344960] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE.
Strain: JD7-7C.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae."
Park S.G., Cha M.-K., Jeong W., Kim I.-H.
J. Biol. Chem. 275:5723-5732(2000) [PubMed: 10681558] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin."
Biteau B., Labarre J., Toledano M.B.
Nature 425:980-984(2003) [PubMed: 14586471] [Abstract]
Cited for: DISULFIDE BOND WITH SRX1.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-174, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L14640 Unassigned DNA. Translation: AAA16374.1.
Z46659 Genomic DNA. Translation: CAA86627.1.
BK006946 Genomic DNA. Translation: DAA09870.1.
PIRA47362.
RefSeqNP_013684.1. NM_001182386.1.

3D structure databases

ProteinModelPortalP34760.
SMRP34760. Positions 3-195.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1667N.
IntActP34760. 25 interactions.
MINTMINT-408012.
STRINGP34760.

Protein family/group databases

PeroxiBase4465. Sce2CysPrx01.

2D gel databases

SWISS-2DPAGEP34760.

Proteomic databases

PeptideAtlasP34760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML028W; YML028W; YML028W.
GeneID854980.
KEGGsce:YML028W.
NMPDRfig|4932.3.peg.4725.

Organism-specific databases

SGDS000004490. TSA1.

Phylogenomic databases

eggNOGfuNOG07930.
GeneTreeEFGT00050000006473.
HOGENOMHBG493509.
OMAVIRHITI.
OrthoDBEOG4SN4Z0.

Gene expression databases

ArrayExpressP34760.
GenevestigatorP34760.
GermOnlineYML028W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KOK03386.
PfamPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFPIRSF000239. AHPC. 1 hit.
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978097.

Entry information

Entry nameTSA1_YEAST
AccessionPrimary (citable) accession number: P34760
Secondary accession number(s): D6VZE6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents