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Protein

3-phytase B

Gene

phyB

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of inorganic orthophosphate from phytate.

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821NucleophileBy similarity
Active sitei338 – 3381Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
3-phytase B (EC:3.1.3.8)
Alternative name(s):
3 phytase B
Myo-inositol hexakisphosphate phosphohydrolase B
Myo-inositol-hexaphosphate 3-phosphohydrolase B
Gene namesi
Name:phyB
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Pathology & Biotechi

Protein family/group databases

Allergomei3131. Asp n 25.0101.
844. Asp n 25.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Chaini20 – 4794603-phytase BPRO_0000023974Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP34754.
SMRiP34754. Positions 33-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG260296.

Family and domain databases

Gene3Di3.40.50.1240. 3 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRTSLLTLA CALATGASAF SYGAAIPQST QEKQFSQEFR DGYSILKHYG
60 70 80 90 100
GNGPYSERVS YGIARDPPTG CEVDQVIMVK RHGERYPSPS AGKSIEEALA
110 120 130 140 150
KVYSINTTEY KGDLAFLNDW TYYVPNECYY NAETTSGPYA GLLDAYNHGN
160 170 180 190 200
DYKARYGHLW NGETVVPFFS SGYGRVIETA RKFGEGFFGY NYSTNAALNI
210 220 230 240 250
ISESEVMGAD SLTPTCDTDN DQTTCDNLTY QLPQFKVAAA RLNSQNPGMN
260 270 280 290 300
LTASDVYNLI VMASFELNAR PFSNWINAFT QDEWVSFGYV EDLNYYYCAG
310 320 330 340 350
PGDKNMAAVG AVYANASLTL LNQGPKEAGP LFFNFAHDTN ITPILAALGV
360 370 380 390 400
LIPNEDLPLD RVAFGNPYSI GNIVPMGGHL TIERLSCQAT ALSDKGTYVR
410 420 430 440 450
LVLNEAVLPF NDCTSGPGYS CPLANYTSIL NKNLPDYTTT CNVSASYPQY
460 470
LSFWWNYNTT TELNYRSSPI ACQEGDAMD
Length:479
Mass (Da):52,612
Last modified:February 1, 1994 - v1
Checksum:i395D4DA2B50FDFC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20567 Unassigned DNA. Translation: AAA02934.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20567 Unassigned DNA. Translation: AAA02934.1.

3D structure databases

ProteinModelPortaliP34754.
SMRiP34754. Positions 33-479.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3131. Asp n 25.0101.
844. Asp n 25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG260296.

Family and domain databases

Gene3Di3.40.50.1240. 3 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum)."
    Ehrlich K.C., Montalbano B.G., Mullaney E.J., Dischinger H.C. Jr., Ullah A.H.J.
    Biochem. Biophys. Res. Commun. 195:53-57(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-101; 133-141 AND 376-399.

Entry informationi

Entry nameiPHYB_ASPNG
AccessioniPrimary (citable) accession number: P34754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.