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Protein

3-phytase A

Gene

phyA

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of inorganic orthophosphate from phytate.

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei82NucleophileBy similarity1
Active sitei362Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.3.26. 518.
3.1.3.8. 518.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phytase A (EC:3.1.3.8)
Alternative name(s):
3 phytase A
Myo-inositol hexakisphosphate phosphohydrolase A
Myo-inositol-hexaphosphate 3-phosphohydrolase A
Gene namesi
Name:phyA
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Is used as a food and feed additive. It can facilitate the degradation of phytin in soybean and other seeds used as food for monogastric animals. Sold by Novo Nordisk under the name Phytase Novo.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000002397024 – 4673-phytase AAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...)1
Disulfide bondi31 ↔ 40
Glycosylationi59N-linked (GlcNAc...)1
Disulfide bondi71 ↔ 414
Glycosylationi105N-linked (GlcNAc...)1
Glycosylationi120N-linked (GlcNAc...)1
Glycosylationi207N-linked (GlcNAc...)1
Disulfide bondi215 ↔ 465
Glycosylationi230N-linked (GlcNAc...)1
Disulfide bondi264 ↔ 282
Glycosylationi339N-linked (GlcNAc...)1
Glycosylationi352N-linked (GlcNAc...)1
Glycosylationi376N-linked (GlcNAc...)1
Glycosylationi388N-linked (GlcNAc...)1
Disulfide bondi436 ↔ 444

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP34752.

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00003968.

Structurei

Secondary structure

1467
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni34 – 36Combined sources3
Helixi42 – 45Combined sources4
Helixi49 – 51Combined sources3
Helixi58 – 60Combined sources3
Beta strandi71 – 81Combined sources11
Helixi89 – 105Combined sources17
Helixi111 – 117Combined sources7
Beta strandi126 – 128Combined sources3
Helixi130 – 146Combined sources17
Helixi148 – 151Combined sources4
Beta strandi157 – 163Combined sources7
Helixi164 – 182Combined sources19
Beta strandi198 – 201Combined sources4
Helixi216 – 220Combined sources5
Helixi223 – 232Combined sources10
Turni233 – 235Combined sources3
Helixi236 – 246Combined sources11
Helixi254 – 268Combined sources15
Helixi272 – 275Combined sources4
Helixi280 – 284Combined sources5
Helixi287 – 304Combined sources18
Turni310 – 312Combined sources3
Helixi313 – 316Combined sources4
Helixi317 – 328Combined sources12
Beta strandi335 – 337Combined sources3
Helixi340 – 343Combined sources4
Turni346 – 348Combined sources3
Beta strandi354 – 360Combined sources7
Helixi362 – 371Combined sources10
Turni372 – 377Combined sources6
Beta strandi383 – 385Combined sources3
Turni389 – 394Combined sources6
Helixi397 – 400Combined sources4
Beta strandi401 – 403Combined sources3
Beta strandi406 – 414Combined sources9
Beta strandi421 – 426Combined sources6
Beta strandi434 – 436Combined sources3
Helixi446 – 452Combined sources7
Helixi454 – 457Combined sources4
Turni458 – 461Combined sources4
Helixi462 – 465Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IHPX-ray2.50A30-467[»]
3K4PX-ray2.40A/B24-467[»]
3K4QX-ray2.20A/B24-467[»]
ProteinModelPortaliP34752.
SMRiP34752.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34752.

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1382. Eukaryota.
ENOG4111FS8. LUCA.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVSAVLLPL YLLSGVTSGL AVPASRNQSS CDTVDQGYQC FSETSHLWGQ
60 70 80 90 100
YAPFFSLANE SVISPEVPAG CRVTFAQVLS RHGARYPTDS KGKKYSALIE
110 120 130 140 150
EIQQNATTFD GKYAFLKTYN YSLGADDLTP FGEQELVNSG IKFYQRYESL
160 170 180 190 200
TRNIVPFIRS SGSSRVIASG KKFIEGFQST KLKDPRAQPG QSSPKIDVVI
210 220 230 240 250
SEASSSNNTL DPGTCTVFED SELADTVEAN FTATFVPSIR QRLENDLSGV
260 270 280 290 300
TLTDTEVTYL MDMCSFDTIS TSTVDTKLSP FCDLFTHDEW INYDYLQSLK
310 320 330 340 350
KYYGHGAGNP LGPTQGVGYA NELIARLTHS PVHDDTSSNH TLDSSPATFP
360 370 380 390 400
LNSTLYADFS HDNGIISILF ALGLYNGTKP LSTTTVENIT QTDGFSSAWT
410 420 430 440 450
VPFASRLYVE MMQCQAEQEP LVRVLVNDRV VPLHGCPVDA LGRCTRDSFV
460
RGLSFARSGG DWAECFA
Length:467
Mass (Da):51,086
Last modified:February 1, 1994 - v1
Checksum:i88FE8F3584341D6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z16414 Genomic DNA. Translation: CAA78904.1.
M94550 Genomic DNA. Translation: AAA32705.1.
PIRiJN0482.
JN0656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z16414 Genomic DNA. Translation: CAA78904.1.
M94550 Genomic DNA. Translation: AAA32705.1.
PIRiJN0482.
JN0656.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IHPX-ray2.50A30-467[»]
3K4PX-ray2.40A/B24-467[»]
3K4QX-ray2.20A/B24-467[»]
ProteinModelPortaliP34752.
SMRiP34752.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5061.CADANGAP00003968.

Proteomic databases

PaxDbiP34752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1382. Eukaryota.
ENOG4111FS8. LUCA.

Enzyme and pathway databases

BRENDAi3.1.3.26. 518.
3.1.3.8. 518.

Miscellaneous databases

EvolutionaryTraceiP34752.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHYA_ASPNG
AccessioniPrimary (citable) accession number: P34752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.