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P34742

- E13A_HORVU

UniProt

P34742 - E13A_HORVU

Protein

Glucan endo-1,3-beta-glucosidase GI

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides. Does not hydrolyze (1,3;1,4)-beta-D-glucans, (1,6)-beta-D-glucan, CM-cellulose, insoluble (1,3)-beta-D-glucans or aryl beta-D-glycosides.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    pH dependencei

    Optimum pH is 4.8.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei234 – 2341NucleophileBy similarity
    Active sitei291 – 2911Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    SABIO-RKP34742.

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase GI (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase GI
    (1->3)-beta-glucanase isoenzyme GI
    Beta-1,3-endoglucanase GI
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP34742.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Glucan endo-1,3-beta-glucosidase GIPRO_0000205273Add
    BLAST

    Expressioni

    Tissue specificityi

    Young leaves and roots.

    Developmental stagei

    First detected in young leaves ten days after initiation of germination. Also detected in young roots but not in mature leaves or roots.

    Gene expression databases

    GenevestigatoriP34742.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP34742.
    SMRiP34742. Positions 2-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34742-1 [UniParc]FASTAAdd to Basket

    « Hide

    TIGVCYGVVA NNLPPANEVV QLYRSNGLTG MRIYFADAKA LSALRGSGIG    50
    LILDVGGNDV LASLAANASN AANWVRDNVR PYYPAVNIKY IAAGNEVWGG 100
    DTQNIVPAMR NLGAALKAPG LGTIKVSTSI RFDAVTNTFP PSNGVFAQAY 150
    MTDVARLLAS TGAPLLTNVY PYFAYKDNPR DIQLNYATFR PGTTTVRDPN 200
    TGLTSQCLFD AMVDAVVAAL ERSGAPGVRV VVSESGWPSA SGFAATADNA 250
    RAYNQGLIDH VGGGTPKRPG ALETYIFAMF NENFKTGELT EKHFGLFNPD 300
    KSPAYPIRFQ 310
    Length:310
    Mass (Da):32,954
    Last modified:October 1, 1994 - v2
    Checksum:iCD10EF40C3A232C2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 383ADA → TDT AA sequence 1 PublicationCurated
    Sequence conflicti45 – 451R → S AA sequence 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96938 mRNA. Translation: AAA32960.1.
    PIRiJC1434.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96938 mRNA. Translation: AAA32960.1 .
    PIRi JC1434.

    3D structure databases

    ProteinModelPortali P34742.
    SMRi P34742. Positions 2-309.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P34742.

    Enzyme and pathway databases

    SABIO-RK P34742.

    Gene expression databases

    Genevestigatori P34742.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare."
      Xu P., Wang J., Fincher G.B.
      Gene 120:157-165(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-310.
      Strain: cv. Clipper.
      Tissue: Leaf and Root.
    2. "Isolation and characterization of a (1-->3)-beta-glucan endohydrolase from germinating barley (Hordeum vulgare): amino acid sequence similarity with barley (1-->3,1-->4)-beta-glucanases."
      Hoej P.B., Slade A.M., Wettenhall R.E.H., Fincher G.B.
      FEBS Lett. 230:67-71(1988)
      Cited for: PROTEIN SEQUENCE OF 1-46.
      Tissue: Seed.
    3. "Purification and properties of three (1-->3)-beta-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare)."
      Hrmova M., Fincher G.B.
      Biochem. J. 289:453-461(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-13, CHARACTERIZATION.

    Entry informationi

    Entry nameiE13A_HORVU
    AccessioniPrimary (citable) accession number: P34742
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3