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Protein

Glucan endo-1,3-beta-glucosidase GI

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides. Does not hydrolyze (1,3;1,4)-beta-D-glucans, (1,6)-beta-D-glucan, CM-cellulose, insoluble (1,3)-beta-D-glucans or aryl beta-D-glycosides.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

pH dependencei

Optimum pH is 4.8.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei234NucleophileBy similarity1
Active sitei291Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

SABIO-RKP34742.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase GI (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase GI
(1->3)-beta-glucanase isoenzyme GI
Beta-1,3-endoglucanase GI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002052731 – 310Glucan endo-1,3-beta-glucosidase GIAdd BLAST310

Expressioni

Tissue specificityi

Young leaves and roots.

Developmental stagei

First detected in young leaves ten days after initiation of germination. Also detected in young roots but not in mature leaves or roots.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP34742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TIGVCYGVVA NNLPPANEVV QLYRSNGLTG MRIYFADAKA LSALRGSGIG
60 70 80 90 100
LILDVGGNDV LASLAANASN AANWVRDNVR PYYPAVNIKY IAAGNEVWGG
110 120 130 140 150
DTQNIVPAMR NLGAALKAPG LGTIKVSTSI RFDAVTNTFP PSNGVFAQAY
160 170 180 190 200
MTDVARLLAS TGAPLLTNVY PYFAYKDNPR DIQLNYATFR PGTTTVRDPN
210 220 230 240 250
TGLTSQCLFD AMVDAVVAAL ERSGAPGVRV VVSESGWPSA SGFAATADNA
260 270 280 290 300
RAYNQGLIDH VGGGTPKRPG ALETYIFAMF NENFKTGELT EKHFGLFNPD
310
KSPAYPIRFQ
Length:310
Mass (Da):32,954
Last modified:October 1, 1994 - v2
Checksum:iCD10EF40C3A232C2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36 – 38ADA → TDT AA sequence (Ref. 2) Curated3
Sequence conflicti45R → S AA sequence (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96938 mRNA. Translation: AAA32960.1.
PIRiJC1434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96938 mRNA. Translation: AAA32960.1.
PIRiJC1434.

3D structure databases

ProteinModelPortaliP34742.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP34742.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE13A_HORVU
AccessioniPrimary (citable) accession number: P34742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1994
Last modified: October 5, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.