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P34742

- E13A_HORVU

UniProt

P34742 - E13A_HORVU

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Protein
Glucan endo-1,3-beta-glucosidase GI
Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides. Does not hydrolyze (1,3;1,4)-beta-D-glucans, (1,6)-beta-D-glucan, CM-cellulose, insoluble (1,3)-beta-D-glucans or aryl beta-D-glycosides.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

pH dependencei

Optimum pH is 4.8.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei234 – 2341Nucleophile By similarity
Active sitei291 – 2911Proton donor By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

SABIO-RKP34742.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase GI (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase GI
(1->3)-beta-glucanase isoenzyme GI
Beta-1,3-endoglucanase GI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP34742.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Glucan endo-1,3-beta-glucosidase GI
PRO_0000205273Add
BLAST

Expressioni

Tissue specificityi

Young leaves and roots.

Developmental stagei

First detected in young leaves ten days after initiation of germination. Also detected in young roots but not in mature leaves or roots.

Gene expression databases

GenevestigatoriP34742.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP34742.
SMRiP34742. Positions 2-309.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34742-1 [UniParc]FASTAAdd to Basket

« Hide

TIGVCYGVVA NNLPPANEVV QLYRSNGLTG MRIYFADAKA LSALRGSGIG    50
LILDVGGNDV LASLAANASN AANWVRDNVR PYYPAVNIKY IAAGNEVWGG 100
DTQNIVPAMR NLGAALKAPG LGTIKVSTSI RFDAVTNTFP PSNGVFAQAY 150
MTDVARLLAS TGAPLLTNVY PYFAYKDNPR DIQLNYATFR PGTTTVRDPN 200
TGLTSQCLFD AMVDAVVAAL ERSGAPGVRV VVSESGWPSA SGFAATADNA 250
RAYNQGLIDH VGGGTPKRPG ALETYIFAMF NENFKTGELT EKHFGLFNPD 300
KSPAYPIRFQ 310
Length:310
Mass (Da):32,954
Last modified:October 1, 1994 - v2
Checksum:iCD10EF40C3A232C2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 383ADA → TDT AA sequence 1 Publication
Sequence conflicti45 – 451R → S AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96938 mRNA. Translation: AAA32960.1.
PIRiJC1434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96938 mRNA. Translation: AAA32960.1 .
PIRi JC1434.

3D structure databases

ProteinModelPortali P34742.
SMRi P34742. Positions 2-309.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P34742.

Enzyme and pathway databases

SABIO-RK P34742.

Gene expression databases

Genevestigatori P34742.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare."
    Xu P., Wang J., Fincher G.B.
    Gene 120:157-165(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-310.
    Strain: cv. Clipper.
    Tissue: Leaf and Root.
  2. "Isolation and characterization of a (1-->3)-beta-glucan endohydrolase from germinating barley (Hordeum vulgare): amino acid sequence similarity with barley (1-->3,1-->4)-beta-glucanases."
    Hoej P.B., Slade A.M., Wettenhall R.E.H., Fincher G.B.
    FEBS Lett. 230:67-71(1988)
    Cited for: PROTEIN SEQUENCE OF 1-46.
    Tissue: Seed.
  3. "Purification and properties of three (1-->3)-beta-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare)."
    Hrmova M., Fincher G.B.
    Biochem. J. 289:453-461(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-13, CHARACTERIZATION.

Entry informationi

Entry nameiE13A_HORVU
AccessioniPrimary (citable) accession number: P34742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1994
Last modified: October 16, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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