Reviewed, UniProtKB/Swiss-Prot P34742 (E13A_HORVU)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase GI     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase GI     (1->3)-beta-glucanase isoenzyme GI     Beta-1,3-endoglucanase GI
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May provide a degree of protection against microbial invasion of germinated barley grain through its ability to degrade fungal cell wall polysaccharides. Does not hydrolyze (1,3;1,4)-beta-D-glucans, (1,6)-beta-D-glucan, CM-cellulose, insoluble (1,3)-beta-D-glucans or aryl beta-D-glycosides.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subunit structure	Monomer.
Tissue specificity	Young leaves and roots.
Developmental stage	First detected in young leaves ten days after initiation of germination. Also detected in young roots but not in mature leaves or roots.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.
Biophysicochemical properties	pH dependence: Optimum pH is 4.8.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Glucan endo-1,3-beta-glucosidase GI		PRO_0000205273
Sites
	Active site	234	1	Nucleophile By similarity
	Active site	291	1	Proton donor By similarity
Experimental info
	Sequence conflict	36 – 38	3	ADA → TDT AA sequence Ref.2
	Sequence conflict	45	1	R → S AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P34742-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: CD10EF40C3A232C2	FASTA	310	32,954
	10 20 30 40 50 60 TIGVCYGVVA NNLPPANEVV QLYRSNGLTG MRIYFADAKA LSALRGSGIG LILDVGGNDV 70 80 90 100 110 120 LASLAANASN AANWVRDNVR PYYPAVNIKY IAAGNEVWGG DTQNIVPAMR NLGAALKAPG 130 140 150 160 170 180 LGTIKVSTSI RFDAVTNTFP PSNGVFAQAY MTDVARLLAS TGAPLLTNVY PYFAYKDNPR 190 200 210 220 230 240 DIQLNYATFR PGTTTVRDPN TGLTSQCLFD AMVDAVVAAL ERSGAPGVRV VVSESGWPSA 250 260 270 280 290 300 SGFAATADNA RAYNQGLIDH VGGGTPKRPG ALETYIFAMF NENFKTGELT EKHFGLFNPD 310 KSPAYPIRFQ

References

[1]	"Evolution and differential expression of the (1-->3)-beta-glucan endohydrolase-encoding gene family in barley, Hordeum vulgare." Xu P., Wang J., Fincher G.B. Gene 120:157-165(1992) [PubMed: 1398132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-310. Strain: cv. Clipper. Tissue: Leaf and Root.
[2]	"Isolation and characterization of a (1-->3)-beta-glucan endohydrolase from germinating barley (Hordeum vulgare): amino acid sequence similarity with barley (1-->3,1-->4)-beta-glucanases." Hoej P.B., Slade A.M., Wettenhall R.E.H., Fincher G.B. FEBS Lett. 230:67-71(1988) Cited for: PROTEIN SEQUENCE OF 1-46. Tissue: Seed.
[3]	"Purification and properties of three (1-->3)-beta-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare)." Hrmova M., Fincher G.B. Biochem. J. 289:453-461(1993) [PubMed: 8424790] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-13, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M96938 mRNA. Translation: AAA32960.1.
PIR	JC1434.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
SMR	P34742. Positions 2-309.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Organism-specific databases
Gramene	P34742.
Enzyme and pathway databases
BRENDA	3.2.1.39. 283.
Gene expression databases
Genevestigator	P34742.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13A_HORVU

Accession

Primary (citable) accession number: P34742

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1994
Last modified:	October 13, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)