P34730 (BMH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BMH2

Gene names

Name:	BMH2
Ordered Locus Names:	YDR099W
ORF Names:	YD8557.08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with FIN1. Ref.8
Subcellular location	Cytoplasm. Nucleus Ref.9.
Miscellaneous	Present with 47600 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Inferred from mutant phenotype. Source: SGD DNA-dependent DNA replication initiation Inferred from genetic interaction. Source: SGD Ras protein signal transduction Inferred from genetic interaction. Source: SGD ascospore formation Inferred from genetic interaction. Source: SGD fungal-type cell wall chitin biosynthetic process Inferred from genetic interaction. Source: SGD glycogen metabolic process Inferred from genetic interaction. Source: SGD negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Inferred from physical interaction. Source: SGD pre-replicative complex assembly Inferred from genetic interaction. Source: SGD pseudohyphal growth Inferred from genetic interaction. Source: SGD signal transduction involved in filamentous growth Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	DNA replication origin binding Inferred from direct assay. Source: SGD phosphoserine binding Inferred from mutant phenotype. Source: SGD protein domain specific binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 273	272	Protein BMH2		PRO_0000058716
Regions
	Compositional bias	245 – 261	17	Poly-Gln
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.7
	Modified residue	104	1	Phosphoserine Ref.11
	Modified residue	212	1	Phosphoserine Ref.11
Experimental info
	Sequence conflict	119	1	S → C in CAA59275. Ref.1
	Sequence conflict	174	1	G → F in CAA59275. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34730 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 74788A9103F129FB	FASTA	273	31,061
	10 20 30 40 50 60 MSQTREDSVY LAKLAEQAER YEEMVENMKA VASSGQELSV EERNLLSVAY KNVIGARRAS 70 80 90 100 110 120 WRIVSSIEQK EESKEKSEHQ VELIRSYRSK IETELTKISD DILSVLDSHL IPSATTGESK 130 140 150 160 170 180 VFYYKMKGDY HRYLAEFSSG DAREKATNSS LEAYKTASEI ATTELPPTHP IRLGLALNFS 190 200 210 220 230 240 VFYYEIQNSP DKACHLAKQA FDDAIAELDT LSEESYKDST LIMQLLRDNL TLWTSDISES 250 260 270 GQEDQQQQQQ QQQQQQQQQQ QAPAEQTQGE PTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 14-3-3 proteins encoded by the BMH1 and BMH2 genes are essential in the yeast Saccharomyces cerevisiae and can be replaced by a plant homologue." van Heusden G.P.H., Griffith D.J.F., Ford J.C., Chin-A-Woeng T.F.C., Schrader P.A.T., Carr A.M., Steensma H.Y. Eur. J. Biochem. 229:45-53(1995) [PubMed: 7744048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae." Gelperin D., Weigle J., Nelson K.K., Roseboom P., Irie K., Matsumoto K., Lemmon S.K. Proc. Natl. Acad. Sci. U.S.A. 92:11539-11543(1995) [PubMed: 8524799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Genome-wide identification of spliced introns using a tiling microarray." Zhang Z., Hesselberth J.R., Fields S. Genome Res. 17:503-509(2007) [PubMed: 17351133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-143. Strain: ATCC 201390 / BY4743.
[6]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed: 17244705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-116. Strain: ATCC 201390 / BY4743.
[7]	"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT SER-2.
[8]	"Self-association of the spindle pole body-related intermediate filament protein Fin1p and its phosphorylation-dependent interaction with 14-3-3 proteins in yeast." van Hemert M.J., Deelder A.M., Molenaar C., Steensma H.Y., van Heusden G.P.H. J. Biol. Chem. 278:15049-15055(2003) [PubMed: 12551942] [Abstract] Cited for: INTERACTION WITH FIN1.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104 AND SER-212, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84817 Genomic DNA. Translation: CAA59275.1. U01883 Unassigned DNA. Translation: AAA03336.1. Z47746 Genomic DNA. Translation: CAA87675.1. DQ881448 mRNA. Translation: ABI95875.1. EF123145 mRNA. Translation: ABM97489.1. BK006938 Genomic DNA. Translation: DAA11946.1.
PIR	S51250.
RefSeq	NP_010384.1. NM_001180407.1.
3D structure databases
ProteinModelPortal	P34730.
SMR	P34730. Positions 5-234.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1212N.
IntAct	P34730. 32 interactions.
MINT	MINT-397916.
STRING	P34730.
2D gel databases
SWISS-2DPAGE	P34730.
Proteomic databases
PeptideAtlas	P34730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR099W; YDR099W; YDR099W.
GeneID	851676.
KEGG	sce:YDR099W.
NMPDR	fig|4932.3.peg.1132.
Organism-specific databases
SGD	S000002506. BMH2.
Phylogenomic databases
eggNOG	fuNOG05351.
GeneTree	EFGT00050000004854.
HOGENOM	HBG611720.
OMA	DEIKEAP.
OrthoDB	EOG4VX5F8.
Gene expression databases
ArrayExpress	P34730.
Genevestigator	P34730.
GermOnline	YDR099W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
Gene3D	G3DSA:1.20.190.20. 14-3-3. 1 hit.
KO	K06630.
PANTHER	PTHR18860. 14-3-3. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969306.

Entry information

Entry name

BMH2_YEAST

Accession

Primary (citable) accession number: P34730
Secondary accession number(s): A2TBP2, D6VS86, Q06HN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program