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P34722 (KPC1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase C-like 1
Short name=PKC
EC=2.7.11.13
Alternative name(s):
Tetradecanoyl phorbol acetate-resistant protein 1
Gene names
Name:tpa-1
ORF Names:B0545.1
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: P34722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: P34722-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 704704Protein kinase C-like 1
PRO_0000055734

Regions

Domain375 – 634260Protein kinase
Domain635 – 70470AGC-kinase C-terminal
Zinc finger165 – 21551Phorbol-ester/DAG-type 1
Zinc finger237 – 28751Phorbol-ester/DAG-type 2
Nucleotide binding381 – 3899ATP By similarity

Sites

Active site4991Proton acceptor By similarity
Binding site4041ATP By similarity

Amino acid modifications

Modified residue891Phosphothreonine; by autocatalysis Potential
Modified residue1391Phosphothreonine; by autocatalysis Potential
Modified residue3241Phosphothreonine; by autocatalysis Potential

Natural variations

Alternative sequence1 – 137137Missing in isoform b.
VSP_004744

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: C44F2E25F58057E3

FASTA70480,298
        10         20         30         40         50         60 
MAQAENACRL KLLRADVPVD LLPAGCSATD LQPAVNVKEK IEVNGESRLV QKKKTLYPEW 

        70         80         90        100        110        120 
EKCWDTAVAE GRILQIVLMF NQTPVVEATM RLEDIISKCK SDAITHIWIN TKPNGRILAQ 

       130        140        150        160        170        180 
TRHLKNAPDD DHPVEDIMTS RSNSGPGIQR RRGAIKHARV HEIRGHQFVA TFFRQPHFCS 

       190        200        210        220        230        240 
LCSDFMWGLN KQGYQCQLCS AAVHKKCHEK VIMQCPGSAK NTKETMALKE RFKVDIPHRF 

       250        260        270        280        290        300 
KTYNFKSPTF CDHCGSMLYG LFKQGLRCEV CNVACHHKCE RLMSNLCGVN QKQLSEMYHE 

       310        320        330        340        350        360 
IKRGTHATAS CPPNIANLHL NGETSKNNGS LPNKLKNLFK SHQYSVEEQK ETDEYMDNIW 

       370        380        390        400        410        420 
GGGDGPVKKF ALPHFNLLKV LGKGSFGKVM LVELKGKNEF YAMKCLKKDV ILEDDDTECT 

       430        440        450        460        470        480 
YIERRVLILA SQCPFLCQLF CSFQTNEYLF FVMEYLNGGD LMHHIQQIKK FDEARTRFYA 

       490        500        510        520        530        540 
CEIVVALQFL HTNNIIYRDL KLDNVLLDCD GHIKLADFGM AKTEMNRENG MASTFCGTPD 

       550        560        570        580        590        600 
YISPEIIKGQ LYNEAVDFWS FGVLMYEMLV GQSPFHGEGE DELFDSILNE RPYFPKTISK 

       610        620        630        640        650        660 
EAAKCLSALF DRNPNTRLGM PECPDGPIRQ HCFFRGVDWK RFENRQVPPP FKPNIKSNSD 

       670        680        690        700 
ASNFDDDFTN EKAALTPVHD KNLLASIDPE AFLNFSYTNP HFSK

« Hide

Isoform b [UniParc].

Checksum: DDB9114F649E590E
Show »

FASTA56764,919

References

« Hide 'large scale' references
[1]"The tpa-1 gene of Caenorhabditis elegans encodes two proteins similar to Ca(2+)-independent protein kinase Cs: evidence by complete genomic and complementary DNA sequences of the tpa-1 gene."
Sano T., Tabuse Y., Nishiwaki K., Miwa J.
J. Mol. Biol. 251:477-485(1995) [PubMed: 7658466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"Mutations in a protein kinase C homolog confer phorbol ester resistance on Caenorhabditis elegans."
Tabuse Y., Nishiwaki K., Miwa J.
Science 243:1713-1716(1989) [PubMed: 2538925] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 148-704.
[4]Miwa J.
Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D49525 Genomic DNA. Translation: BAA08470.1.
D49525 Genomic DNA. Translation: BAA08471.1.
FO080226 Genomic DNA. Translation: CCD62171.1.
FO080226 Genomic DNA. Translation: CCD62172.1.
D14815 mRNA. Translation: BAA03556.1.
PIRS60117.
T33400.
RefSeqNP_499860.1. NM_067459.2.
NP_499861.1. NM_067460.3.
UniGeneCel.12259.

3D structure databases

ProteinModelPortalP34722.
SMRP34722. Positions 5-126, 150-219, 238-287, 367-703.
ModBaseSearch...

Protein-protein interaction databases

STRINGP34722.

Proteomic databases

PRIDEP34722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaB0545.1a; B0545.1a; B0545.1.
GeneID176825.
KEGGcel:B0545.1.
UCSCB0545.1a. c. elegans.

Organism-specific databases

CTD176825.
WormBaseB0545.1a; CE17353; WBGene00006599; tpa-1.
B0545.1b; CE17354; WBGene00006599; tpa-1.

Phylogenomic databases

eggNOGmeNOG04064.
GeneTreeEMGT00050000001097.
HOGENOMHBG755340.
InParanoidP34722.
OMAAITHIWI.
PhylomeDBP34722.

Enzyme and pathway databases

BRENDA2.7.11.13. 1045.

Gene expression databases

ArrayExpressP34722.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR002290. Ser/Thr_kinase_dom.
[Graphical view]
KOK06068.
PfamPF00130. C1_1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFPIRSF000551. PKC_delta. 1 hit.
PRINTSPR00008. DAGPEDOMAIN.
SMARTSM00109. C1. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF49562. C2_CaLB. 1 hit.
SSF56112. Kinase_like. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio894172.

Entry information

Entry nameKPC1_CAEEL
AccessionPrimary (citable) accession number: P34722
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: December 14, 2011
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents