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Protein

Protein kinase C-like 1

Gene

tpa-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Diacylglycerol (DAG)-dependent serine/threonine-protein kinase that phosphorylates a range of cellular proteins (Probable). Phosphorylates mlk-1, a component of the JNK pathway. Involved in axon regeneration after injury probably by activating the JNK pathway (PubMed:23072806). Plays a role in resistance to fungal infection and in wound healing by promoting expression of antimicrobial peptide nlp-29 in the epidermis downstream of gpa-12 and plc-3 and upstream of tir-1-p38-like pathway (PubMed:22470487, PubMed:19380113). Probably by regulating neuronal transmission in ALA neurons, regulates the decrease in pharyngeal pumping during the quiescent state that precedes each larval molt, downstream of lin-3 and receptor let-23 and phospholipase plc-3 (PubMed:17891142).Curated4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei404ATPPROSITE-ProRule annotation1
Active sitei499Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri165 – 215Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri237 – 287Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi381 – 389ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • defense response to fungus Source: WormBase
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • response to wounding Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13. 1045.
ReactomeiR-CEL-111933. Calmodulin induced events.
R-CEL-114508. Effects of PIP2 hydrolysis.
R-CEL-1489509. DAG and IP3 signaling.
R-CEL-202424. Downstream TCR signaling.
R-CEL-2029485. Role of phospholipids in phagocytosis.
R-CEL-2871837. FCERI mediated NF-kB activation.
R-CEL-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-CEL-5218921. VEGFR2 mediated cell proliferation.
R-CEL-5607764. CLEC7A (Dectin-1) signaling.
R-CEL-6798695. Neutrophil degranulation.
SignaLinkiP34722.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C-like 1 (EC:2.7.11.13)
Short name:
PKC
Alternative name(s):
Tetradecanoyl phorbol acetate-resistant protein 1
Gene namesi
Name:tpa-1
ORF Names:B0545.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiB0545.1a; CE17353; WBGene00006599; tpa-1.
B0545.1b; CE17354; WBGene00006599; tpa-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Up-regulation of nlp-29 is severely impaired in the hyp7 and vulva epidermal cells following fungal infection by D.coniospora or physical injury.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi384G → E: Severe loss of nlp-29 expression upon D.coniospora infection. 1 Publication1
Mutagenesisi404K → R: Probable loss of kinase activity. Loss of mlk-1 phosphorylation. 1 Publication1
Mutagenesisi544P → S in k501; normal up-regulation of nlp-29 upon D.coniospora infection or physical injury. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557341 – 704Protein kinase C-like 1Add BLAST704

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89Phosphothreonine; by autocatalysisSequence analysis1
Modified residuei139Phosphothreonine; by autocatalysisSequence analysis1
Modified residuei324Phosphothreonine; by autocatalysisSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP34722.
PaxDbiP34722.
PeptideAtlasiP34722.
PRIDEiP34722.

PTM databases

iPTMnetiP34722.

Expressioni

Gene expression databases

BgeeiWBGene00006599.

Interactioni

Protein-protein interaction databases

STRINGi6239.B0545.1a.

Structurei

3D structure databases

ProteinModelPortaliP34722.
SMRiP34722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini375 – 634Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini635 – 704AGC-kinase C-terminalAdd BLAST70

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri165 – 215Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri237 – 287Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
InParanoidiP34722.
KOiK06068.
OMAiITHIWIN.
OrthoDBiEOG091G0QRS.
PhylomeDBiP34722.

Family and domain databases

CDDicd00029. C1. 2 hits.
Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P34722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQAENACRL KLLRADVPVD LLPAGCSATD LQPAVNVKEK IEVNGESRLV
60 70 80 90 100
QKKKTLYPEW EKCWDTAVAE GRILQIVLMF NQTPVVEATM RLEDIISKCK
110 120 130 140 150
SDAITHIWIN TKPNGRILAQ TRHLKNAPDD DHPVEDIMTS RSNSGPGIQR
160 170 180 190 200
RRGAIKHARV HEIRGHQFVA TFFRQPHFCS LCSDFMWGLN KQGYQCQLCS
210 220 230 240 250
AAVHKKCHEK VIMQCPGSAK NTKETMALKE RFKVDIPHRF KTYNFKSPTF
260 270 280 290 300
CDHCGSMLYG LFKQGLRCEV CNVACHHKCE RLMSNLCGVN QKQLSEMYHE
310 320 330 340 350
IKRGTHATAS CPPNIANLHL NGETSKNNGS LPNKLKNLFK SHQYSVEEQK
360 370 380 390 400
ETDEYMDNIW GGGDGPVKKF ALPHFNLLKV LGKGSFGKVM LVELKGKNEF
410 420 430 440 450
YAMKCLKKDV ILEDDDTECT YIERRVLILA SQCPFLCQLF CSFQTNEYLF
460 470 480 490 500
FVMEYLNGGD LMHHIQQIKK FDEARTRFYA CEIVVALQFL HTNNIIYRDL
510 520 530 540 550
KLDNVLLDCD GHIKLADFGM AKTEMNRENG MASTFCGTPD YISPEIIKGQ
560 570 580 590 600
LYNEAVDFWS FGVLMYEMLV GQSPFHGEGE DELFDSILNE RPYFPKTISK
610 620 630 640 650
EAAKCLSALF DRNPNTRLGM PECPDGPIRQ HCFFRGVDWK RFENRQVPPP
660 670 680 690 700
FKPNIKSNSD ASNFDDDFTN EKAALTPVHD KNLLASIDPE AFLNFSYTNP

HFSK
Length:704
Mass (Da):80,298
Last modified:November 1, 1997 - v2
Checksum:iC44F2E25F58057E3
GO
Isoform b (identifier: P34722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.

Show »
Length:567
Mass (Da):64,919
Checksum:iDDB9114F649E590E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0047441 – 137Missing in isoform b. CuratedAdd BLAST137

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49525 Genomic DNA. Translation: BAA08470.1.
D49525 Genomic DNA. Translation: BAA08471.1.
FO080226 Genomic DNA. Translation: CCD62171.1.
FO080226 Genomic DNA. Translation: CCD62172.1.
D14815 mRNA. Translation: BAA03556.1.
PIRiS60117.
T33400.
RefSeqiNP_499860.1. NM_067459.3. [P34722-1]
NP_499861.1. NM_067460.4. [P34722-2]
UniGeneiCel.12259.

Genome annotation databases

EnsemblMetazoaiB0545.1a; B0545.1a; WBGene00006599. [P34722-1]
GeneIDi176825.
KEGGicel:CELE_B0545.1.
UCSCiB0545.1a. c. elegans. [P34722-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49525 Genomic DNA. Translation: BAA08470.1.
D49525 Genomic DNA. Translation: BAA08471.1.
FO080226 Genomic DNA. Translation: CCD62171.1.
FO080226 Genomic DNA. Translation: CCD62172.1.
D14815 mRNA. Translation: BAA03556.1.
PIRiS60117.
T33400.
RefSeqiNP_499860.1. NM_067459.3. [P34722-1]
NP_499861.1. NM_067460.4. [P34722-2]
UniGeneiCel.12259.

3D structure databases

ProteinModelPortaliP34722.
SMRiP34722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.B0545.1a.

PTM databases

iPTMnetiP34722.

Proteomic databases

EPDiP34722.
PaxDbiP34722.
PeptideAtlasiP34722.
PRIDEiP34722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0545.1a; B0545.1a; WBGene00006599. [P34722-1]
GeneIDi176825.
KEGGicel:CELE_B0545.1.
UCSCiB0545.1a. c. elegans. [P34722-1]

Organism-specific databases

CTDi176825.
WormBaseiB0545.1a; CE17353; WBGene00006599; tpa-1.
B0545.1b; CE17354; WBGene00006599; tpa-1.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
InParanoidiP34722.
KOiK06068.
OMAiITHIWIN.
OrthoDBiEOG091G0QRS.
PhylomeDBiP34722.

Enzyme and pathway databases

BRENDAi2.7.11.13. 1045.
ReactomeiR-CEL-111933. Calmodulin induced events.
R-CEL-114508. Effects of PIP2 hydrolysis.
R-CEL-1489509. DAG and IP3 signaling.
R-CEL-202424. Downstream TCR signaling.
R-CEL-2029485. Role of phospholipids in phagocytosis.
R-CEL-2871837. FCERI mediated NF-kB activation.
R-CEL-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-CEL-5218921. VEGFR2 mediated cell proliferation.
R-CEL-5607764. CLEC7A (Dectin-1) signaling.
R-CEL-6798695. Neutrophil degranulation.
SignaLinkiP34722.

Miscellaneous databases

PROiP34722.

Gene expression databases

BgeeiWBGene00006599.

Family and domain databases

CDDicd00029. C1. 2 hits.
Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPC1_CAEEL
AccessioniPrimary (citable) accession number: P34722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.