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P34721

- MT1B_MORBO

UniProt

P34721 - MT1B_MORBO

Protein

Modification methylase MboIB

Gene

mboIBM

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3668. M2.MboI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase MboIB (EC:2.1.1.72)
    Short name:
    M.MboIB
    Alternative name(s):
    Adenine-specific methyltransferase MboIB
    M.MboI C
    Gene namesi
    Name:mboIBM
    Synonyms:mboC
    OrganismiMoraxella bovis
    Taxonomic identifieri476 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273Modification methylase MboIBPRO_0000087956Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP34721.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34721-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIKPYFESD DKNFNIYQGN CIDFMSHFQD NSIDMIFADP PYFLSNDGLT    50
    FKNSIIQSVN KGEWDKNDNE ASIYNFNHEW IAQARQLLKD NGTIWISGTH 100
    HNIFTVGQVL KENNFKILNI ITWEKPNPPP NFSCRYFTYS SEWIIWARKH 150
    SKIPHYFNYD LMKKLNGDKQ QKDIWRLPAV GSWEKTQGKH PTQKPLGLLS 200
    RIILSSTQKD DLILDPFSGS GTTGIAGVLL DRNYIGIEQE LEFLELSKRR 250
    YHEITPVLKN EFKQKIRKQI SAI 273
    Length:273
    Mass (Da):31,923
    Last modified:February 1, 1994 - v1
    Checksum:i9571B82CB6B55D93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13968 Genomic DNA. Translation: BAA03073.1.
    PIRiS35647.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13968 Genomic DNA. Translation: BAA03073.1 .
    PIRi S35647.

    3D structure databases

    ProteinModelPortali P34721.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3668. M2.MboI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure and expression of the MboI restriction-modification system."
      Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
      Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

    Entry informationi

    Entry nameiMT1B_MORBO
    AccessioniPrimary (citable) accession number: P34721
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The MboI restriction system has two different methylases.

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3