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P34721

- MT1B_MORBO

UniProt

P34721 - MT1B_MORBO

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Protein

Modification methylase MboIB

Gene

mboIBM

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3668. M2.MboI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MboIB (EC:2.1.1.72)
Short name:
M.MboIB
Alternative name(s):
Adenine-specific methyltransferase MboIB
M.MboI C
Gene namesi
Name:mboIBM
Synonyms:mboC
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Modification methylase MboIBPRO_0000087956Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP34721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34721-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIKPYFESD DKNFNIYQGN CIDFMSHFQD NSIDMIFADP PYFLSNDGLT
60 70 80 90 100
FKNSIIQSVN KGEWDKNDNE ASIYNFNHEW IAQARQLLKD NGTIWISGTH
110 120 130 140 150
HNIFTVGQVL KENNFKILNI ITWEKPNPPP NFSCRYFTYS SEWIIWARKH
160 170 180 190 200
SKIPHYFNYD LMKKLNGDKQ QKDIWRLPAV GSWEKTQGKH PTQKPLGLLS
210 220 230 240 250
RIILSSTQKD DLILDPFSGS GTTGIAGVLL DRNYIGIEQE LEFLELSKRR
260 270
YHEITPVLKN EFKQKIRKQI SAI
Length:273
Mass (Da):31,923
Last modified:February 1, 1994 - v1
Checksum:i9571B82CB6B55D93
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13968 Genomic DNA. Translation: BAA03073.1.
PIRiS35647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13968 Genomic DNA. Translation: BAA03073.1 .
PIRi S35647.

3D structure databases

ProteinModelPortali P34721.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3668. M2.MboI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Gene structure and expression of the MboI restriction-modification system."
    Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
    Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Entry informationi

Entry nameiMT1B_MORBO
AccessioniPrimary (citable) accession number: P34721
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The MboI restriction system has two different methylases.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3