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P34721 (MT1B_MORBO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase MboIB

Short name=M.MboIB
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase MboIB
M.MboI C
Gene names
Name:mboIBM
Synonyms:mboC
OrganismMoraxella bovis
Taxonomic identifier476 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Miscellaneous

The MboI restriction system has two different methylases.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Modification methylase MboIB
PRO_0000087956

Sequences

Sequence LengthMass (Da)Tools
P34721 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9571B82CB6B55D93

FASTA27331,923
        10         20         30         40         50         60 
MRIKPYFESD DKNFNIYQGN CIDFMSHFQD NSIDMIFADP PYFLSNDGLT FKNSIIQSVN 

        70         80         90        100        110        120 
KGEWDKNDNE ASIYNFNHEW IAQARQLLKD NGTIWISGTH HNIFTVGQVL KENNFKILNI 

       130        140        150        160        170        180 
ITWEKPNPPP NFSCRYFTYS SEWIIWARKH SKIPHYFNYD LMKKLNGDKQ QKDIWRLPAV 

       190        200        210        220        230        240 
GSWEKTQGKH PTQKPLGLLS RIILSSTQKD DLILDPFSGS GTTGIAGVLL DRNYIGIEQE 

       250        260        270 
LEFLELSKRR YHEITPVLKN EFKQKIRKQI SAI 

« Hide

References

[1]"Gene structure and expression of the MboI restriction-modification system."
Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13968 Genomic DNA. Translation: BAA03073.1.
PIRS35647.

3D structure databases

ProteinModelPortalP34721.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3668. M2.MboI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMT1B_MORBO
AccessionPrimary (citable) accession number: P34721
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries