P34721 (MT1B_MORBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase MboIB
Short name=M.MboIB
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase MboIB
M.MboI C

Gene names

Name:	mboIBM
Synonyms:	mboC

Organism

Moraxella bovis

Taxonomic identifier

476 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Moraxella › Moraxella

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Miscellaneous	The MboI restriction system has two different methylases.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Modification methylase MboIB		PRO_0000087956

Sequences

	Sequence		Length	Mass (Da)
	P34721 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 9571B82CB6B55D93	FASTA	273	31,923
	10 20 30 40 50 60 MRIKPYFESD DKNFNIYQGN CIDFMSHFQD NSIDMIFADP PYFLSNDGLT FKNSIIQSVN 70 80 90 100 110 120 KGEWDKNDNE ASIYNFNHEW IAQARQLLKD NGTIWISGTH HNIFTVGQVL KENNFKILNI 130 140 150 160 170 180 ITWEKPNPPP NFSCRYFTYS SEWIIWARKH SKIPHYFNYD LMKKLNGDKQ QKDIWRLPAV 190 200 210 220 230 240 GSWEKTQGKH PTQKPLGLLS RIILSSTQKD DLILDPFSGS GTTGIAGVLL DRNYIGIEQE 250 260 270 LEFLELSKRR YHEITPVLKN EFKQKIRKQI SAI

References

[1]

"Gene structure and expression of the MboI restriction-modification system." Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K. Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13968 Genomic DNA. Translation: BAA03073.1.
PIR	S35647.
3D structure databases
ProteinModelPortal	P34721.
ModBase	Search...
Protein family/group databases
REBASE	3668. M2.MboI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT1B_MORBO

Accession

Primary (citable) accession number: P34721

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program