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P34720

- MT1A_MORBO

UniProt

P34720 - MT1A_MORBO

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Protein

Modification methylase MboIA

Gene
mboIAM, mboA
Organism
Moraxella bovis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionine By similarity
Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei61 – 611S-adenosyl-L-methionine By similarity
Binding sitei198 – 1981S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3667. M1.MboI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MboIA (EC:2.1.1.72)
Short name:
M.MboIA
Alternative name(s):
Adenine-specific methyltransferase MboIA
Gene namesi
Name:mboIAM
Synonyms:mboA
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Modification methylase MboIAPRO_0000087955Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP34720.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.

Sequencei

Sequence statusi: Complete.

P34720-1 [UniParc]FASTAAdd to Basket

« Hide

MKPFIKWAGG KNSLLDEIQK RLPDFVHSQD FCLVEPFVGG GAVSLWALSD    50
LPHLKQLVIN DCNADLINVY QVIKNNPDDL IGYIENLQSH YDKLTDLESK 100
KPYFYHKRDV FNQRTSNDIE QAGLFIFLNK SAFNGLYRVN KNNQFNVPIG 150
NYKKPTFVDK ENILNISKKL QNTKILSGDF ELVLAHLPNN FPCLFYLDPP 200
YRPISDTASF TSYSDNGFDD NEQKRLANFC KKIDKLGHYF LLSNSDPKNT 250
NSSDEFFDEL YQDFKIERIQ ANRTISANSN GRKKVNEIIV SNGV 294
Length:294
Mass (Da):33,767
Last modified:February 1, 1994 - v1
Checksum:iE042441B46C6BB04
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13968 Genomic DNA. Translation: BAA03071.1.
PIRiS35646.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13968 Genomic DNA. Translation: BAA03071.1 .
PIRi S35646.

3D structure databases

ProteinModelPortali P34720.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3667. M1.MboI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene structure and expression of the MboI restriction-modification system."
    Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
    Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Entry informationi

Entry nameiMT1A_MORBO
AccessioniPrimary (citable) accession number: P34720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The MboI restriction system has two different methylases.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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