Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P34720 (MT1A_MORBO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase MboIA

Short name=M.MboIA
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase MboIA
Gene names
Name:mboIAM
Synonyms:mboA
OrganismMoraxella bovis
Taxonomic identifier476 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Miscellaneous

The MboI restriction system has two different methylases.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionsite-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Modification methylase MboIA
PRO_0000087955

Sites

Binding site71S-adenosyl-L-methionine By similarity
Binding site111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site1981S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
P34720 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: E042441B46C6BB04

FASTA29433,767
        10         20         30         40         50         60 
MKPFIKWAGG KNSLLDEIQK RLPDFVHSQD FCLVEPFVGG GAVSLWALSD LPHLKQLVIN 

        70         80         90        100        110        120 
DCNADLINVY QVIKNNPDDL IGYIENLQSH YDKLTDLESK KPYFYHKRDV FNQRTSNDIE 

       130        140        150        160        170        180 
QAGLFIFLNK SAFNGLYRVN KNNQFNVPIG NYKKPTFVDK ENILNISKKL QNTKILSGDF 

       190        200        210        220        230        240 
ELVLAHLPNN FPCLFYLDPP YRPISDTASF TSYSDNGFDD NEQKRLANFC KKIDKLGHYF 

       250        260        270        280        290 
LLSNSDPKNT NSSDEFFDEL YQDFKIERIQ ANRTISANSN GRKKVNEIIV SNGV 

« Hide

References

[1]"Gene structure and expression of the MboI restriction-modification system."
Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13968 Genomic DNA. Translation: BAA03071.1.
PIRS35646.

3D structure databases

ProteinModelPortalP34720.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3667. M1.MboI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
TIGRFAMsTIGR00571. dam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMT1A_MORBO
AccessionPrimary (citable) accession number: P34720
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries