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Reviewed, UniProtKB/Swiss-Prot P34720 (MT1A_MORBO)

Last modified November 3, 2009. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Modification methylase MboIA
      Short name=M.MboIA
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase MboIA
Gene names
Name: mboIAM
Synonyms: mboA
OrganismMoraxella bovis
Taxonomic identifier476 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Miscellaneous

The MboI restriction system has two different methylases.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Modification methylase MboIA
PRO_0000087955

Sites

Binding site71S-adenosyl-L-methionine By similarity
Binding site111S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site1981S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
P34720-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: E042441B46C6BB04

FASTA29433,767
        10         20         30         40         50         60 
MKPFIKWAGG KNSLLDEIQK RLPDFVHSQD FCLVEPFVGG GAVSLWALSD LPHLKQLVIN 

        70         80         90        100        110        120 
DCNADLINVY QVIKNNPDDL IGYIENLQSH YDKLTDLESK KPYFYHKRDV FNQRTSNDIE 

       130        140        150        160        170        180 
QAGLFIFLNK SAFNGLYRVN KNNQFNVPIG NYKKPTFVDK ENILNISKKL QNTKILSGDF 

       190        200        210        220        230        240 
ELVLAHLPNN FPCLFYLDPP YRPISDTASF TSYSDNGFDD NEQKRLANFC KKIDKLGHYF 

       250        260        270        280        290 
LLSNSDPKNT NSSDEFFDEL YQDFKIERIQ ANRTISANSN GRKKVNEIIV SNGV 

« Hide

References

[1]"Gene structure and expression of the MboI restriction-modification system."
Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
Nucleic Acids Res. 21:2309-2313(1993) [PubMed: 8506128] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases

D13968 Genomic DNA. Translation: BAA03071.1.
PIRS35646.

3D structure databases

HSSPHSSP built from PDB template 2DPM based on UniProtKB P04043.
ModBaseSearch...

Protein family/group databases

REBASE3667. M1.MboI.

Enzyme and pathway databases

BRENDA2.1.1.72. 96192.

Family and domain databases

InterProIPR002294. D12N6_MeTrfase.
IPR012326. Dam.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSPR00505. D12N6MTFRASE.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMT1A_MORBO
AccessionPrimary (citable) accession number: P34720
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents