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P34720

- MT1A_MORBO

UniProt

P34720 - MT1A_MORBO

Protein

Modification methylase MboIA

Gene

mboIAM

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71S-adenosyl-L-methionineBy similarity
    Binding sitei11 – 111S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei61 – 611S-adenosyl-L-methionineBy similarity
    Binding sitei198 – 1981S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3667. M1.MboI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase MboIA (EC:2.1.1.72)
    Short name:
    M.MboIA
    Alternative name(s):
    Adenine-specific methyltransferase MboIA
    Gene namesi
    Name:mboIAM
    Synonyms:mboA
    OrganismiMoraxella bovis
    Taxonomic identifieri476 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 294294Modification methylase MboIAPRO_0000087955Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP34720.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34720-1 [UniParc]FASTAAdd to Basket

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    MKPFIKWAGG KNSLLDEIQK RLPDFVHSQD FCLVEPFVGG GAVSLWALSD    50
    LPHLKQLVIN DCNADLINVY QVIKNNPDDL IGYIENLQSH YDKLTDLESK 100
    KPYFYHKRDV FNQRTSNDIE QAGLFIFLNK SAFNGLYRVN KNNQFNVPIG 150
    NYKKPTFVDK ENILNISKKL QNTKILSGDF ELVLAHLPNN FPCLFYLDPP 200
    YRPISDTASF TSYSDNGFDD NEQKRLANFC KKIDKLGHYF LLSNSDPKNT 250
    NSSDEFFDEL YQDFKIERIQ ANRTISANSN GRKKVNEIIV SNGV 294
    Length:294
    Mass (Da):33,767
    Last modified:February 1, 1994 - v1
    Checksum:iE042441B46C6BB04
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13968 Genomic DNA. Translation: BAA03071.1.
    PIRiS35646.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13968 Genomic DNA. Translation: BAA03071.1 .
    PIRi S35646.

    3D structure databases

    ProteinModelPortali P34720.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3667. M1.MboI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure and expression of the MboI restriction-modification system."
      Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
      Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

    Entry informationi

    Entry nameiMT1A_MORBO
    AccessioniPrimary (citable) accession number: P34720
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The MboI restriction system has two different methylases.

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3