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Protein

Type-2 restriction enzyme MboI

Gene

mboIR

Organism
Moraxella bovis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Protein family/group databases

REBASEi1204. MboI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MboI (EC:3.1.21.4)
Short name:
R.MboI
Alternative name(s):
Endonuclease MboI
Type II restriction enzyme MboI
Gene namesi
Name:mboIR
Synonyms:mboB
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773301 – 280Type-2 restriction enzyme MboIAdd BLAST280

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR011335. Restrct_endonuc-II-like.
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
PfamiView protein in Pfam
PF04556. DpnII. 1 hit.
PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P34719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAFDDFLN SMSETNTTLD YFTDFDKVKK NVAQIEIHLN QLNYLLGKDD
60 70 80 90 100
LKQAVYDLYA ECPNAFSILE ILIAVRKKEQ KKSLDEKGQV VTLNSYFQSA
110 120 130 140 150
DKIIDFLNNT GLADVFRDKN IKNLVDYVFG IEVGLDTNAR KNRGGDNMSK
160 170 180 190 200
AVQLLFDNAD IYYKKEVRNT IFTDIESLGA DVKQFDFVIK TKRKTYVIET
210 220 230 240 250
NYYNSGGSKL NEVARAYTDV APKINQYSQY EFVWITDGQG WKTAKNKLQE
260 270 280
AYTHIPSVYN LYTLHGFIEQ LNSEGVIKDW
Length:280
Mass (Da):32,248
Last modified:February 1, 1994 - v1
Checksum:iD208B25B5C078C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13968 Genomic DNA. Translation: BAA03072.1.
PIRiS35648.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2M1_MORBO
AccessioniPrimary (citable) accession number: P34719
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families