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P34719

- T2M1_MORBO

UniProt

P34719 - T2M1_MORBO

Protein

Type-2 restriction enzyme MboI

Gene

mboIR

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1204. MboI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MboI (EC:3.1.21.4)
    Short name:
    R.MboI
    Alternative name(s):
    Endonuclease MboI
    Type II restriction enzyme MboI
    Gene namesi
    Name:mboIR
    Synonyms:mboB
    OrganismiMoraxella bovis
    Taxonomic identifieri476 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 280280Type-2 restriction enzyme MboIPRO_0000077330Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR021191. Restrct_endonuc_II_DpmII.
    IPR007637. Restrct_endonuc_II_DpmII-like.
    [Graphical view]
    PfamiPF04556. DpnII. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P34719-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLAFDDFLN SMSETNTTLD YFTDFDKVKK NVAQIEIHLN QLNYLLGKDD    50
    LKQAVYDLYA ECPNAFSILE ILIAVRKKEQ KKSLDEKGQV VTLNSYFQSA 100
    DKIIDFLNNT GLADVFRDKN IKNLVDYVFG IEVGLDTNAR KNRGGDNMSK 150
    AVQLLFDNAD IYYKKEVRNT IFTDIESLGA DVKQFDFVIK TKRKTYVIET 200
    NYYNSGGSKL NEVARAYTDV APKINQYSQY EFVWITDGQG WKTAKNKLQE 250
    AYTHIPSVYN LYTLHGFIEQ LNSEGVIKDW 280
    Length:280
    Mass (Da):32,248
    Last modified:February 1, 1994 - v1
    Checksum:iD208B25B5C078C72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13968 Genomic DNA. Translation: BAA03072.1.
    PIRiS35648.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13968 Genomic DNA. Translation: BAA03072.1 .
    PIRi S35648.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1204. MboI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR021191. Restrct_endonuc_II_DpmII.
    IPR007637. Restrct_endonuc_II_DpmII-like.
    [Graphical view ]
    Pfami PF04556. DpnII. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure and expression of the MboI restriction-modification system."
      Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
      Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-30.
      Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

    Entry informationi

    Entry nameiT2M1_MORBO
    AccessioniPrimary (citable) accession number: P34719
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3