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P34719

- T2M1_MORBO

UniProt

P34719 - T2M1_MORBO

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Protein

Type-2 restriction enzyme MboI

Gene

mboIR

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1204. MboI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MboI (EC:3.1.21.4)
Short name:
R.MboI
Alternative name(s):
Endonuclease MboI
Type II restriction enzyme MboI
Gene namesi
Name:mboIR
Synonyms:mboB
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Type-2 restriction enzyme MboIPRO_0000077330Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamiPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P34719-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLAFDDFLN SMSETNTTLD YFTDFDKVKK NVAQIEIHLN QLNYLLGKDD
60 70 80 90 100
LKQAVYDLYA ECPNAFSILE ILIAVRKKEQ KKSLDEKGQV VTLNSYFQSA
110 120 130 140 150
DKIIDFLNNT GLADVFRDKN IKNLVDYVFG IEVGLDTNAR KNRGGDNMSK
160 170 180 190 200
AVQLLFDNAD IYYKKEVRNT IFTDIESLGA DVKQFDFVIK TKRKTYVIET
210 220 230 240 250
NYYNSGGSKL NEVARAYTDV APKINQYSQY EFVWITDGQG WKTAKNKLQE
260 270 280
AYTHIPSVYN LYTLHGFIEQ LNSEGVIKDW
Length:280
Mass (Da):32,248
Last modified:February 1, 1994 - v1
Checksum:iD208B25B5C078C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13968 Genomic DNA. Translation: BAA03072.1.
PIRiS35648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13968 Genomic DNA. Translation: BAA03072.1 .
PIRi S35648.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1204. MboI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011335. Restrct_endonuc-II-like.
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view ]
Pfami PF04556. DpnII. 1 hit.
[Graphical view ]
PIRSFi PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene structure and expression of the MboI restriction-modification system."
    Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
    Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-30.
    Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Entry informationi

Entry nameiT2M1_MORBO
AccessioniPrimary (citable) accession number: P34719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3