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P34719

- T2M1_MORBO

UniProt

P34719 - T2M1_MORBO

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Protein
Type-2 restriction enzyme MboI
Gene
mboIR, mboB
Organism
Moraxella bovis
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1204. MboI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MboI (EC:3.1.21.4)
Short name:
R.MboI
Alternative name(s):
Endonuclease MboI
Type II restriction enzyme MboI
Gene namesi
Name:mboIR
Synonyms:mboB
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Type-2 restriction enzyme MboI
PRO_0000077330Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamiPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P34719-1 [UniParc]FASTAAdd to Basket

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MKLAFDDFLN SMSETNTTLD YFTDFDKVKK NVAQIEIHLN QLNYLLGKDD    50
LKQAVYDLYA ECPNAFSILE ILIAVRKKEQ KKSLDEKGQV VTLNSYFQSA 100
DKIIDFLNNT GLADVFRDKN IKNLVDYVFG IEVGLDTNAR KNRGGDNMSK 150
AVQLLFDNAD IYYKKEVRNT IFTDIESLGA DVKQFDFVIK TKRKTYVIET 200
NYYNSGGSKL NEVARAYTDV APKINQYSQY EFVWITDGQG WKTAKNKLQE 250
AYTHIPSVYN LYTLHGFIEQ LNSEGVIKDW 280
Length:280
Mass (Da):32,248
Last modified:February 1, 1994 - v1
Checksum:iD208B25B5C078C72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13968 Genomic DNA. Translation: BAA03072.1.
PIRiS35648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13968 Genomic DNA. Translation: BAA03072.1 .
PIRi S35648.

3D structure databases

ModBasei Search...

Protein family/group databases

REBASEi 1204. MboI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011335. Restrct_endonuc-II-like.
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view ]
Pfami PF04556. DpnII. 1 hit.
[Graphical view ]
PIRSFi PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene structure and expression of the MboI restriction-modification system."
    Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
    Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-30.
    Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Entry informationi

Entry nameiT2M1_MORBO
AccessioniPrimary (citable) accession number: P34719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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