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P34719 (T2M1_MORBO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MboI

Short name=R.MboI
EC=3.1.21.4
Alternative name(s):
Endonuclease MboI
Type II restriction enzyme MboI
Gene names
Name:mboIR
Synonyms:mboB
OrganismMoraxella bovis
Taxonomic identifier476 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sequence similarities

Belongs to the type II restriction enzyme DpnII family.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 280280Type-2 restriction enzyme MboI
PRO_0000077330

Sequences

Sequence LengthMass (Da)Tools
P34719 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: D208B25B5C078C72

FASTA28032,248
        10         20         30         40         50         60 
MKLAFDDFLN SMSETNTTLD YFTDFDKVKK NVAQIEIHLN QLNYLLGKDD LKQAVYDLYA 

        70         80         90        100        110        120 
ECPNAFSILE ILIAVRKKEQ KKSLDEKGQV VTLNSYFQSA DKIIDFLNNT GLADVFRDKN 

       130        140        150        160        170        180 
IKNLVDYVFG IEVGLDTNAR KNRGGDNMSK AVQLLFDNAD IYYKKEVRNT IFTDIESLGA 

       190        200        210        220        230        240 
DVKQFDFVIK TKRKTYVIET NYYNSGGSKL NEVARAYTDV APKINQYSQY EFVWITDGQG 

       250        260        270        280 
WKTAKNKLQE AYTHIPSVYN LYTLHGFIEQ LNSEGVIKDW 

« Hide

References

[1]"Gene structure and expression of the MboI restriction-modification system."
Ueno T., Ito H., Kimizuka F., Kotani H., Nakajima K.
Nucleic Acids Res. 21:2309-2313(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-30.
Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13968 Genomic DNA. Translation: BAA03072.1.
PIRS35648.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1204. MboI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011335. Restrct_endonuc-II-like.
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2M1_MORBO
AccessionPrimary (citable) accession number: P34719
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries