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Protein

Multidrug resistance protein pgp-1

Gene

pgp-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi451 – 4588ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1112 – 11198ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • defense response to Gram-negative bacterium Source: WormBase
  • innate immune response Source: WormBase
  • response to metal ion Source: WormBase
  • response to nematicide Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.201.14. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein pgp-1 (EC:3.6.3.44)
Alternative name(s):
P-glycoprotein A
P-glycoprotein-related protein 1
Gene namesi
Name:pgp-1
ORF Names:K08E7.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiK08E7.9; CE11932; WBGene00003995; pgp-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7777CytoplasmicSequence analysisAdd
BLAST
Transmembranei78 – 9821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei144 – 16421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei213 – 23321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei240 – 26021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei321 – 34121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei350 – 37021HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini371 – 753383CytoplasmicSequence analysisAdd
BLAST
Transmembranei754 – 77421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei798 – 81821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei874 – 89421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei895 – 91521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei978 – 99821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei1017 – 103721HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini1038 – 1321284CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13211321Multidrug resistance protein pgp-1PRO_0000093342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence analysis
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Glycosylationi850 – 8501N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP34712.
PaxDbiP34712.
PRIDEiP34712.

PTM databases

iPTMnetiP34712.

Expressioni

Tissue specificityi

Intestinal cells.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.K08E7.9.

Structurei

Secondary structure

1
1321
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1612Combined sources
Helixi21 – 3313Combined sources
Turni37 – 393Combined sources
Beta strandi42 – 443Combined sources
Helixi62 – 654Combined sources
Turni66 – 683Combined sources
Helixi71 – 8818Combined sources
Helixi90 – 11324Combined sources
Helixi128 – 17952Combined sources
Helixi184 – 1896Combined sources
Helixi195 – 20814Combined sources
Helixi212 – 23423Combined sources
Helixi236 – 2438Combined sources
Helixi246 – 28338Combined sources
Helixi285 – 2906Combined sources
Helixi294 – 34552Combined sources
Turni346 – 3483Combined sources
Helixi352 – 39241Combined sources
Turni393 – 3953Combined sources
Beta strandi402 – 4043Combined sources
Beta strandi416 – 4238Combined sources
Beta strandi426 – 4283Combined sources
Beta strandi433 – 4419Combined sources
Beta strandi446 – 4516Combined sources
Beta strandi453 – 4553Combined sources
Helixi457 – 4648Combined sources
Beta strandi471 – 4777Combined sources
Turni482 – 4843Combined sources
Helixi487 – 4937Combined sources
Beta strandi494 – 4974Combined sources
Beta strandi505 – 5073Combined sources
Helixi508 – 5136Combined sources
Helixi521 – 53010Combined sources
Helixi534 – 5396Combined sources
Turni541 – 5444Combined sources
Beta strandi545 – 5528Combined sources
Helixi557 – 56913Combined sources
Beta strandi574 – 5807Combined sources
Turni581 – 5844Combined sources
Turni587 – 5893Combined sources
Helixi590 – 60112Combined sources
Beta strandi604 – 6096Combined sources
Turni613 – 6186Combined sources
Beta strandi620 – 6267Combined sources
Beta strandi629 – 6346Combined sources
Helixi636 – 6405Combined sources
Turni641 – 6433Combined sources
Helixi645 – 66319Combined sources
Helixi722 – 7287Combined sources
Turni729 – 7324Combined sources
Helixi740 – 7467Combined sources
Helixi747 – 7504Combined sources
Helixi751 – 76313Combined sources
Helixi764 – 7663Combined sources
Helixi767 – 78115Combined sources
Turni788 – 7925Combined sources
Helixi793 – 83846Combined sources
Beta strandi842 – 8443Combined sources
Helixi848 – 8503Combined sources
Helixi852 – 8609Combined sources
Helixi862 – 8676Combined sources
Turni868 – 8703Combined sources
Helixi872 – 89423Combined sources
Helixi896 – 91621Combined sources
Turni925 – 9273Combined sources
Helixi930 – 94314Combined sources
Helixi945 – 9506Combined sources
Turni951 – 9533Combined sources
Helixi954 – 98633Combined sources
Helixi989 – 100315Combined sources
Beta strandi1006 – 10094Combined sources
Helixi1014 – 102512Combined sources
Turni1026 – 10283Combined sources
Helixi1033 – 10364Combined sources
Helixi1037 – 105620Combined sources
Beta strandi1077 – 10848Combined sources
Beta strandi1094 – 11029Combined sources
Beta strandi1107 – 11115Combined sources
Helixi1121 – 11255Combined sources
Beta strandi1132 – 11387Combined sources
Turni1143 – 11453Combined sources
Helixi1148 – 11525Combined sources
Beta strandi1155 – 11584Combined sources
Beta strandi1166 – 11683Combined sources
Helixi1169 – 11735Combined sources
Beta strandi1174 – 11774Combined sources
Turni1179 – 11813Combined sources
Helixi1184 – 119310Combined sources
Helixi1197 – 12015Combined sources
Turni1204 – 12074Combined sources
Beta strandi1209 – 12113Combined sources
Turni1212 – 12143Combined sources
Helixi1220 – 123314Combined sources
Beta strandi1237 – 12437Combined sources
Helixi1251 – 126010Combined sources
Turni1261 – 12633Combined sources
Beta strandi1264 – 12729Combined sources
Beta strandi1274 – 12774Combined sources
Turni1278 – 12814Combined sources
Beta strandi1283 – 129715Combined sources
Helixi1299 – 13046Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F4CX-ray3.40A1-1321[»]
ProteinModelPortaliP34712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 381305ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini416 – 652237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini754 – 1043290ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1077 – 1315239ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
InParanoidiP34712.
KOiK05658.
OrthoDBiEOG7Z3F4H.
PhylomeDBiP34712.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34712-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRNGSLRQS LRTLDSFSLA PEDVLKTAIK TVEDYEGDNI DSNGEIKITR
60 70 80 90 100
DAKEEVVNKV SIPQLYRYTT TLEKLLLFIG TLVAVITGAG LPLMSILQGK
110 120 130 140 150
VSQAFINEQI VINNNGSTFL PTGQNYTKTD FEHDVMNVVW SYAAMTVGMW
160 170 180 190 200
AAGQITVTCY LYVAEQMNNR LRREFVKSIL RQEISWFDTN HSGTLATKLF
210 220 230 240 250
DNLERVKEGT GDKIGMAFQY LSQFITGFIV AFTHSWQLTL VMLAVTPIQA
260 270 280 290 300
LCGFAIAKSM STFAIRETLR YAKAGKVVEE TISSIRTVVS LNGLRYELER
310 320 330 340 350
YSTAVEEAKK AGVLKGLFLG ISFGAMQASN FISFALAFYI GVGWVHDGSL
360 370 380 390 400
NFGDMLTTFS SVMMGSMALG LAGPQLAVLG TAQGAASGIY EVLDRKPVID
410 420 430 440 450
SSSKAGRKDM KIKGDITVEN VHFTYPSRPD VPILRGMNLR VNAGQTVALV
460 470 480 490 500
GSSGCGKSTI ISLLLRYYDV LKGKITIDGV DVRDINLEFL RKNVAVVSQE
510 520 530 540 550
PALFNCTIEE NISLGKEGIT REEMVAACKM ANAEKFIKTL PNGYNTLVGD
560 570 580 590 600
RGTQLSGGQK QRIAIARALV RNPKILLLDE ATSALDAESE GIVQQALDKA
610 620 630 640 650
AKGRTTIIIA HRLSTIRNAD LIISCKNGQV VEVGDHRALM AQQGLYYDLV
660 670 680 690 700
TAQTFTDAVD SAAEGKFSRE NSVARQTSEH EGLSRQASEM DDIMNRVRSS
710 720 730 740 750
TIGSITNGPV IDEKEERIGK DALSRLKQEL EENNAQKTNL FEILYHARPH
760 770 780 790 800
ALSLFIGMST ATIGGFIYPT YSVFFTSFMN VFAGNPADFL SQGHFWALMF
810 820 830 840 850
LVLAAAQGIC SFLMTFFMGI ASESLTRDLR NKLFRNVLSQ HIGFFDSPQN
860 870 880 890 900
ASGKISTRLA TDVPNLRTAI DFRFSTVITT LVSMVAGIGL AFFYGWQMAL
910 920 930 940 950
LIIAILPIVA FGQYLRGRRF TGKNVKSASE FADSGKIAIE AIENVRTVQA
960 970 980 990 1000
LAREDTFYEN FCEKLDIPHK EAIKEAFIQG LSYGCASSVL YLLNTCAYRM
1010 1020 1030 1040 1050
GLALIITDPP TMQPMRVLRV MYAITISTST LGFATSYFPE YAKATFAGGI
1060 1070 1080 1090 1100
IFGMLRKISK IDSLSLAGEK KKLYGKVIFK NVRFAYPERP EIEILKGLSF
1110 1120 1130 1140 1150
SVEPGQTLAL VGPSGCGKST VVALLERFYD TLGGEIFIDG SEIKTLNPEH
1160 1170 1180 1190 1200
TRSQIAIVSQ EPTLFDCSIA ENIIYGLDPS SVTMAQVEEA ARLANIHNFI
1210 1220 1230 1240 1250
AELPEGFETR VGDRGTQLSG GQKQRIAIAR ALVRNPKILL LDEATSALDT
1260 1270 1280 1290 1300
ESEKVVQEAL DRAREGRTCI VIAHRLNTVM NADCIAVVSN GTIIEKGTHT
1310 1320
QLMSEKGAYY KLTQKQMTEK K
Length:1,321
Mass (Da):145,063
Last modified:March 28, 2003 - v2
Checksum:i68094121B30E8746
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561T → I in CAA46190 (PubMed:1360540).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65054 Genomic DNA. Translation: CAA46190.1.
Z77666 Genomic DNA. Translation: CAB01232.1.
PIRiS27337.
T23476.
RefSeqiNP_502413.1. NM_070012.4.
UniGeneiCel.12537.

Genome annotation databases

EnsemblMetazoaiK08E7.9; K08E7.9; WBGene00003995.
GeneIDi178215.
KEGGicel:CELE_K08E7.9.
UCSCiK08E7.9. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65054 Genomic DNA. Translation: CAA46190.1.
Z77666 Genomic DNA. Translation: CAB01232.1.
PIRiS27337.
T23476.
RefSeqiNP_502413.1. NM_070012.4.
UniGeneiCel.12537.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F4CX-ray3.40A1-1321[»]
ProteinModelPortaliP34712.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K08E7.9.

Protein family/group databases

TCDBi3.A.1.201.14. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiP34712.

Proteomic databases

EPDiP34712.
PaxDbiP34712.
PRIDEiP34712.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK08E7.9; K08E7.9; WBGene00003995.
GeneIDi178215.
KEGGicel:CELE_K08E7.9.
UCSCiK08E7.9. c. elegans.

Organism-specific databases

CTDi178215.
WormBaseiK08E7.9; CE11932; WBGene00003995; pgp-1.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
InParanoidiP34712.
KOiK05658.
OrthoDBiEOG7Z3F4H.
PhylomeDBiP34712.

Miscellaneous databases

PROiP34712.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The P-glycoprotein gene family of Caenorhabditis elegans. Cloning and characterization of genomic and complementary DNA sequences."
    Lincke C.R., The I., van Groenigen M., Borst P.
    J. Mol. Biol. 228:701-711(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "The expression of two P-glycoprotein (pgp) genes in transgenic Caenorhabditis elegans is confined to intestinal cells."
    Lincke C.R., Broeks A., The I., Plasterk H.A., Borst P.
    EMBO J. 12:1615-1620(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Strain: Bristol N2.

Entry informationi

Entry nameiPGP1_CAEEL
AccessioniPrimary (citable) accession number: P34712
Secondary accession number(s): Q21349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 28, 2003
Last modified: June 8, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.