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Reviewed, UniProtKB/Swiss-Prot P34712 (MDR1_CAEEL)

Last modified January 19, 2010. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Multidrug resistance protein 1
    EC=3.6.3.44
Alternative name(s):
    P-glycoprotein A
Gene names
Name: pgp-1
ORF Names: K08E7.9
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length1321 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Intestinal cells. Ref.3

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtransmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13211321Multidrug resistance protein 1
PRO_0000093342

Regions

Topological domain1 – 7777Cytoplasmic Potential
Transmembrane78 – 9821 Potential
Transmembrane144 – 16421 Potential
Transmembrane213 – 23321 Potential
Transmembrane240 – 26021 Potential
Transmembrane321 – 34121 Potential
Transmembrane350 – 37021 Potential
Topological domain371 – 753383Cytoplasmic Potential
Transmembrane754 – 77421 Potential
Transmembrane798 – 81821 Potential
Transmembrane874 – 89421 Potential
Transmembrane895 – 91521 Potential
Transmembrane978 – 99821 Potential
Transmembrane1017 – 103721 Potential
Topological domain1038 – 1321284Cytoplasmic Potential
Domain77 – 381305ABC transmembrane type-1 1
Domain416 – 652237ABC transporter 1
Domain754 – 1043290ABC transmembrane type-1 2
Domain1077 – 1315239ABC transporter 2
Nucleotide binding451 – 4588ATP 1 Potential
Nucleotide binding1112 – 11198ATP 2 Potential

Amino acid modifications

Glycosylation1151N-linked (GlcNAc...) Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation1901N-linked (GlcNAc...) Potential
Glycosylation8501N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1561T → I in CAA46190. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P34712-1 [UniParc].

Last modified March 28, 2003. Version 2.
Checksum: 68094121B30E8746

FASTA1,321145,063
        10         20         30         40         50         60 
MLRNGSLRQS LRTLDSFSLA PEDVLKTAIK TVEDYEGDNI DSNGEIKITR DAKEEVVNKV 

        70         80         90        100        110        120 
SIPQLYRYTT TLEKLLLFIG TLVAVITGAG LPLMSILQGK VSQAFINEQI VINNNGSTFL 

       130        140        150        160        170        180 
PTGQNYTKTD FEHDVMNVVW SYAAMTVGMW AAGQITVTCY LYVAEQMNNR LRREFVKSIL 

       190        200        210        220        230        240 
RQEISWFDTN HSGTLATKLF DNLERVKEGT GDKIGMAFQY LSQFITGFIV AFTHSWQLTL 

       250        260        270        280        290        300 
VMLAVTPIQA LCGFAIAKSM STFAIRETLR YAKAGKVVEE TISSIRTVVS LNGLRYELER 

       310        320        330        340        350        360 
YSTAVEEAKK AGVLKGLFLG ISFGAMQASN FISFALAFYI GVGWVHDGSL NFGDMLTTFS 

       370        380        390        400        410        420 
SVMMGSMALG LAGPQLAVLG TAQGAASGIY EVLDRKPVID SSSKAGRKDM KIKGDITVEN 

       430        440        450        460        470        480 
VHFTYPSRPD VPILRGMNLR VNAGQTVALV GSSGCGKSTI ISLLLRYYDV LKGKITIDGV 

       490        500        510        520        530        540 
DVRDINLEFL RKNVAVVSQE PALFNCTIEE NISLGKEGIT REEMVAACKM ANAEKFIKTL 

       550        560        570        580        590        600 
PNGYNTLVGD RGTQLSGGQK QRIAIARALV RNPKILLLDE ATSALDAESE GIVQQALDKA 

       610        620        630        640        650        660 
AKGRTTIIIA HRLSTIRNAD LIISCKNGQV VEVGDHRALM AQQGLYYDLV TAQTFTDAVD 

       670        680        690        700        710        720 
SAAEGKFSRE NSVARQTSEH EGLSRQASEM DDIMNRVRSS TIGSITNGPV IDEKEERIGK 

       730        740        750        760        770        780 
DALSRLKQEL EENNAQKTNL FEILYHARPH ALSLFIGMST ATIGGFIYPT YSVFFTSFMN 

       790        800        810        820        830        840 
VFAGNPADFL SQGHFWALMF LVLAAAQGIC SFLMTFFMGI ASESLTRDLR NKLFRNVLSQ 

       850        860        870        880        890        900 
HIGFFDSPQN ASGKISTRLA TDVPNLRTAI DFRFSTVITT LVSMVAGIGL AFFYGWQMAL 

       910        920        930        940        950        960 
LIIAILPIVA FGQYLRGRRF TGKNVKSASE FADSGKIAIE AIENVRTVQA LAREDTFYEN 

       970        980        990       1000       1010       1020 
FCEKLDIPHK EAIKEAFIQG LSYGCASSVL YLLNTCAYRM GLALIITDPP TMQPMRVLRV 

      1030       1040       1050       1060       1070       1080 
MYAITISTST LGFATSYFPE YAKATFAGGI IFGMLRKISK IDSLSLAGEK KKLYGKVIFK 

      1090       1100       1110       1120       1130       1140 
NVRFAYPERP EIEILKGLSF SVEPGQTLAL VGPSGCGKST VVALLERFYD TLGGEIFIDG 

      1150       1160       1170       1180       1190       1200 
SEIKTLNPEH TRSQIAIVSQ EPTLFDCSIA ENIIYGLDPS SVTMAQVEEA ARLANIHNFI 

      1210       1220       1230       1240       1250       1260 
AELPEGFETR VGDRGTQLSG GQKQRIAIAR ALVRNPKILL LDEATSALDT ESEKVVQEAL 

      1270       1280       1290       1300       1310       1320 
DRAREGRTCI VIAHRLNTVM NADCIAVVSN GTIIEKGTHT QLMSEKGAYY KLTQKQMTEK 


K

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References

« Hide 'large scale' references
[1]"The P-glycoprotein gene family of Caenorhabditis elegans. Cloning and characterization of genomic and complementary DNA sequences."
Lincke C.R., The I., van Groenigen M., Borst P.
J. Mol. Biol. 228:701-711(1992) [PubMed: 1360540] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"The expression of two P-glycoprotein (pgp) genes in transgenic Caenorhabditis elegans is confined to intestinal cells."
Lincke C.R., Broeks A., The I., Plasterk H.A., Borst P.
EMBO J. 12:1615-1620(1993) [PubMed: 8096815] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: Bristol N2.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X65054 Genomic DNA. Translation: CAA46190.1.
Z77666 Genomic DNA. Translation: CAB01232.1.
PIRS27337.
T23476.
RefSeqNP_502413.1.
UniGeneCel.12537

3D structure databases

SMRP34712. Positions 158-654, 796-1316.
ModBaseSearch...

Genome annotation databases

EnsemblK08E7.9; K08E7.9; K08E7.9; Caenorhabditis elegans. [Genome view]
GeneID178215.
KEGGcel:K08E7.9.
NMPDRfig|6239.3.peg.15541.
UCSCK08E7.9. c. elegans.

Organism-specific databases

CTD178215.
WormBaseWBGene00003995. pgp-1.
WormPepK08E7.9. CE11932. [WorfDB]

Phylogenomic databases

eggNOGmeNOG06663.
HOGENOMHBG758042.
InParanoidP34712.
OMAQTSEHEG.
PhylomeDBP34712.

Enzyme and pathway databases

BRENDA3.6.3.44. 672.

Gene expression databases

ArrayExpressP34712.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio900198.

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Entry information

Entry nameMDR1_CAEEL
AccessionPrimary (citable) accession number: P34712
Secondary accession number(s): Q21349
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 28, 2003
Last modified: January 19, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents