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Protein

Synaptotagmin-1

Gene

snt-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone (By similarity). Involved in necrotic cell death (PubMed:22157748).By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi190Calcium 1By similarity1
Metal bindingi190Calcium 2By similarity1
Metal bindingi196Calcium 1By similarity1
Metal bindingi248Calcium 1By similarity1
Metal bindingi248Calcium 2By similarity1
Metal bindingi249Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi250Calcium 1By similarity1
Metal bindingi250Calcium 2By similarity1
Metal bindingi250Calcium 3By similarity1
Metal bindingi253Calcium 3By similarity1
Metal bindingi254Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi256Calcium 2By similarity1
Metal bindingi256Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

  • calcium ion-regulated exocytosis of neurotransmitter Source: GO_Central
  • defecation Source: WormBase
  • locomotory behavior Source: WormBase
  • positive regulation of necrotic cell death Source: WormBase
  • programmed cell death Source: UniProtKB-KW
  • regulation of calcium ion-dependent exocytosis Source: GO_Central
  • regulation of neurotransmitter secretion Source: WormBase
  • regulation of pharyngeal pumping Source: WormBase
  • synaptic vesicle endocytosis Source: WormBase
  • synaptic vesicle exocytosis Source: WormBase
  • vesicle fusion Source: GO_Central

Keywordsi

Biological processNecrosis
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-8856825 Cargo recognition for clathrin-mediated endocytosis
R-CEL-8856828 Clathrin-mediated endocytosis

Protein family/group databases

TCDBi1.F.1.1.3 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-1
Alternative name(s):
Synaptotagmin I
Gene namesi
Name:snt-1
ORF Names:F31E8.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF31E8.2a ; CE02711 ; WBGene00004921 ; snt-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 69VesicularSequence analysisAdd BLAST69
Transmembranei70 – 96HelicalSequence analysisAdd BLAST27
Topological domaini97 – 441CytoplasmicSequence analysisAdd BLAST345

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Worms exhibit severe behavioral abnormalities that are characteristic of deficiencies in synaptic function, including severe locomotion, feeding, and defecation defects (PubMed:8391930). Increased survival in response to hypoxia induced by sodium azide (PubMed:22157748). Reduces the formation of neuron cell corpses in a hyperactive mec-4 or deg-3 mutant background (PubMed:22157748).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839881 – 441Synaptotagmin-1Add BLAST441

Proteomic databases

EPDiP34693
PaxDbiP34693
PeptideAtlasiP34693
PRIDEiP34693

Expressioni

Tissue specificityi

Localized to regions known to be rich in synapses and appears to be associated with synaptic vesicles. Also found in some non-neuronal secretory structures.

Gene expression databases

BgeeiWBGene00004921
ExpressionAtlasiP34693 baseline and differential

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi6239.F31E8.2b

Structurei

3D structure databases

ProteinModelPortaliP34693
SMRiP34693
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 262C2 1PROSITE-ProRule annotationAdd BLAST88
Domaini306 – 397C2 2PROSITE-ProRule annotationAdd BLAST92

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT3Q Eukaryota
ENOG410Z15P LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232127
InParanoidiP34693

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR015428 Synaptotagmin1/2
PANTHERiPTHR10024:SF239 PTHR10024:SF239, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequencei

Sequence statusi: Complete.

P34693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLDFSSQD EENDEDLTKE FVRDEAPMEE TTSEAVKQIA TTTKETLKDV
60 70 80 90 100
VVNKVIDVKD VVKEKVMQQT GMPEWAFVFL GFVFILLVLA CAFCLIRKLF
110 120 130 140 150
GKKRHGEKNK KGGLKGFFGK GQDVVDGKNI QGMAQDLEEL GDAMEQNEKE
160 170 180 190 200
QAEEKEEVKL GRIQYKLDYD FQQGQLTVTV IQAEDLPGMD MSGTSDPYVK
210 220 230 240 250
LYLLPEKKKK VETKVHRKTL NPVFNETFIF KVAFNEITAK TLVFAIYDFD
260 270 280 290 300
RFSKHDQIGQ VLIPLGKIDL GAVIEEWKDI APPPDDKEAE KSLGDICFSL
310 320 330 340 350
RYVPTAGKLT VVILEAKNLK KMDVGGLSDP YVKIVLMQGG KRLKKKKTSI
360 370 380 390 400
KKCTLNPYYN ESFSFEVPFE QIQKVSLMIT VMDYDKLGSN DAIGRCLLGC
410 420 430 440
NGTGAELRHW MDMLASPRRP IAQWHTLGPV EEEGDKKDDK K
Length:441
Mass (Da):49,904
Last modified:February 1, 1994 - v1
Checksum:iF8D174337EB472DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15302 mRNA Translation: AAA28145.1
FO080321 Genomic DNA Translation: CCD62852.1
PIRiA40707
RefSeqiNP_001022129.1, NM_001026958.3
UniGeneiCel.18270

Genome annotation databases

EnsemblMetazoaiF31E8.2a; F31E8.2a; WBGene00004921
GeneIDi174120
UCSCiF31E8.2a c. elegans

Similar proteinsi

Entry informationi

Entry nameiSYT1_CAEEL
AccessioniPrimary (citable) accession number: P34693
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health