Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent RNA helicase glh-1

Gene

glh-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 175CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri183 – 200CCHC-type 2PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri242 – 259CCHC-type 3PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri262 – 279CCHC-type 4PROSITE-ProRule annotationAdd BLAST18
Nucleotide bindingi385 – 392ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • DEAD/H-box RNA helicase binding Source: WormBase
  • JUN kinase binding Source: WormBase
  • protein self-association Source: WormBase
  • RNA binding Source: UniProtKB-KW
  • RNA helicase activity Source: WormBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • germ cell development Source: WormBase
  • post-embryonic development Source: WormBase
  • RNA metabolic process Source: WormBase
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase glh-1 (EC:3.6.4.13)
Alternative name(s):
Germline helicase 1
Gene namesi
Name:glh-1
ORF Names:T21G5.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiT21G5.3; CE25121; WBGene00001598; glh-1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • P granule Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi581K → N: Partially abolishes the interaction with kgb-1; when associated with W-588. No effect on the interaction with kgb-1; when associated with W-589. 1 Publication1
Mutagenesisi588L → W: Partially abolishes the interaction with kgb-1; when associated with N-581. 1 Publication1
Mutagenesisi589L → W: No effect on the interaction with kgb-1; when associated with N-581. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550891 – 763ATP-dependent RNA helicase glh-1Add BLAST763

Post-translational modificationi

Phosphorylated by kgb-1 (in vitro); this may be responsible for its degradation by the proteasome.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP34689.
PaxDbiP34689.
PeptideAtlasiP34689.
PRIDEiP34689.

PTM databases

iPTMnetiP34689.

Expressioni

Developmental stagei

During germline proliferation.

Gene expression databases

BgeeiWBGene00001598.

Interactioni

Subunit structurei

Interacts with csn-5; this may prevent glh-1 degradation induced by kgb-1 (PubMed:12435362, PubMed:17699606). Interacts with zyx-1 (PubMed:12435362). Interacts (via the N-terminal region containing the four CCHC zinc fingers) with pan-1 (PubMed:22342905). Interacts with kgb-1; this may promote glh-1 degradation by the proteasome (PubMed:17699606).1 Publication3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
csn-5P910013EBI-1571791,EBI-313007
glh-3O018362EBI-1571791,EBI-1571750

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: WormBase
  • JUN kinase binding Source: WormBase
  • protein self-association Source: WormBase

Protein-protein interaction databases

BioGridi37860. 4 interactors.
IntActiP34689. 4 interactors.
STRINGi6239.T21G5.3.

Structurei

3D structure databases

ProteinModelPortaliP34689.
SMRiP34689.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati24 – 33110
Repeati34 – 43210
Repeati44 – 53310
Repeati54 – 63410
Repeati64 – 73510
Repeati74 – 83610
Repeati84 – 93710
Domaini372 – 556Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST185
Domaini592 – 739Helicase C-terminalPROSITE-ProRule annotationAdd BLAST148

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 937 X 10 AA tandem repeats, Gly-richAdd BLAST70

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi341 – 369Q motifAdd BLAST29
Motifi423 – 427Phosphodegron1 Publication5
Motifi499 – 502DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi207 – 236Gly-richAdd BLAST30

Sequence similaritiesi

Contains 4 CCHC-type zinc fingers.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri158 – 175CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri183 – 200CCHC-type 2PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri242 – 259CCHC-type 3PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri262 – 279CCHC-type 4PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
InParanoidiP34689.
KOiK13982.
OMAiCTEPRKE.
OrthoDBiEOG091G04U3.
PhylomeDBiP34689.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.60.10. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00098. zf-CCHC. 4 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00343. ZnF_C2HC. 4 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
PS50158. ZF_CCHC. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34689-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGWSDSES AAKAKTGFGS GGGFGGGNNG GSGFGGGKNG GTGFGGGNTG
60 70 80 90 100
GSGFGGGNTG GSGFGGGKTG GSGFGGGNTC GSGFGGGSTG GSPYGGASSG
110 120 130 140 150
FGGSTATSGF GSGEKSSAFG GSGGFGGSAT GFGSGGGSFG GGNSGFGEGG
160 170 180 190 200
HGGGERNNNC FNCQQPGHRS SDCPEPRKER EPRVCYNCQQ PGHTSRECTE
210 220 230 240 250
ERKPREGRTG GFGGGAGFGN NGGNDGFGGD GGFGGGEERG PMKCFNCKGE
260 270 280 290 300
GHRSAECPEP PRGCFNCGEQ GHRSNECPNP AKPREGVEGE GPKATYVPVE
310 320 330 340 350
DNMEDVFNMQ KISEGLMFNK FFDAEVKLTS SEKTVGIKPC KTFAEANLTE
360 370 380 390 400
TMQKNVAHAG YSKTTPIQQY ALPLVHQGYD IMACAQTGSG KTAAFLLPIM
410 420 430 440 450
TRLIDDNNLN TAGEGGCYPR CIILTPTREL ADQIYNEGRK FAYQTMMEIK
460 470 480 490 500
PVYGGLAVGY NKGQIEKGAT IIVGTVGRIK HFCEEGTIKL DKCRFFVLDE
510 520 530 540 550
ADRMIDAMGF GTDIETIVNY DSMPRKENRQ TLMFSATFPD SVQEAARAFL
560 570 580 590 600
RENYVMIAID KIGAANKCVL QEFERCERSE KKDKLLELLG IDIDSYTTEK
610 620 630 640 650
SAEVYTKKTM VFVSQRAMAD TLASILSSAQ VPAITIHGAR EQRERSEALR
660 670 680 690 700
QFRNGSKPVL IATAVAERGL DIKGVDHVIN YDMPDNIDDY IHRIGRTGRV
710 720 730 740 750
GNSGRATSFI SEDCSLLSEL VGVLADAQQI VPDWMQGAAG GNYGASGFGS
760
SVPTQVPQDE EGW
Length:763
Mass (Da):79,780
Last modified:August 16, 2004 - v3
Checksum:i956CADE2937A1725
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69T → A in AAB04136 (PubMed:8943022).Curated1
Sequence conflicti621T → I in AAC27384 (PubMed:8415696).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19948 mRNA. Translation: AAC27384.1.
U62772 Genomic DNA. Translation: AAB04136.1.
FO080897 Genomic DNA. Translation: CCD67591.1.
PIRiA48686.
C87818.
T15132.
RefSeqiNP_491963.1. NM_059562.5.
UniGeneiCel.19653.

Genome annotation databases

EnsemblMetazoaiT21G5.3; T21G5.3; WBGene00001598.
GeneIDi172414.
KEGGicel:CELE_T21G5.3.
UCSCiT21G5.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19948 mRNA. Translation: AAC27384.1.
U62772 Genomic DNA. Translation: AAB04136.1.
FO080897 Genomic DNA. Translation: CCD67591.1.
PIRiA48686.
C87818.
T15132.
RefSeqiNP_491963.1. NM_059562.5.
UniGeneiCel.19653.

3D structure databases

ProteinModelPortaliP34689.
SMRiP34689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37860. 4 interactors.
IntActiP34689. 4 interactors.
STRINGi6239.T21G5.3.

PTM databases

iPTMnetiP34689.

Proteomic databases

EPDiP34689.
PaxDbiP34689.
PeptideAtlasiP34689.
PRIDEiP34689.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT21G5.3; T21G5.3; WBGene00001598.
GeneIDi172414.
KEGGicel:CELE_T21G5.3.
UCSCiT21G5.3. c. elegans.

Organism-specific databases

CTDi172414.
WormBaseiT21G5.3; CE25121; WBGene00001598; glh-1.

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
InParanoidiP34689.
KOiK13982.
OMAiCTEPRKE.
OrthoDBiEOG091G04U3.
PhylomeDBiP34689.

Miscellaneous databases

PROiP34689.

Gene expression databases

BgeeiWBGene00001598.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.60.10. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00098. zf-CCHC. 4 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00343. ZnF_C2HC. 4 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
PS50158. ZF_CCHC. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLH1_CAEEL
AccessioniPrimary (citable) accession number: P34689
Secondary accession number(s): Q22873, Q7KQH5, Q9TXH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.