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P34678

- GALT3_CAEEL

UniProt

P34678 - GALT3_CAEEL

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Protein
Polypeptide N-acetylgalactosaminyltransferase 3
Gene
gly-3, ZK688.8
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei210 – 2101Substrate By similarity
Binding sitei240 – 2401Substrate By similarity
Metal bindingi263 – 2631Manganese By similarity
Metal bindingi265 – 2651Manganese By similarity
Binding sitei370 – 3701Substrate By similarity
Metal bindingi398 – 3981Manganese By similarity
Binding sitei401 – 4011Substrate By similarity
Binding sitei406 – 4061Substrate By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. polypeptide N-acetylgalactosaminyltransferase activity Source: WormBase
Complete GO annotation...

GO - Biological processi

  1. protein O-linked glycosylation via threonine Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 3 (EC:2.4.1.41)
Alternative name(s):
GalNAc-T1
Protein-UDP acetylgalactosaminyltransferase 3
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
Short name:
pp-GaNTase 3
Gene namesi
Name:gly-3
ORF Names:ZK688.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiZK688.8; CE29649; WBGene00001628; gly-3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei14 – 3421Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini35 – 612578Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Polypeptide N-acetylgalactosaminyltransferase 3
PRO_0000059146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi157 ↔ 393 By similarity
Glycosylationi239 – 2391N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi384 ↔ 462 By similarity
Disulfide bondi496 ↔ 513 By similarity
Disulfide bondi536 ↔ 554 By similarity
Disulfide bondi582 ↔ 595 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP34678.

Interactioni

Protein-protein interaction databases

STRINGi6239.ZK688.8.1.

Structurei

3D structure databases

ProteinModelPortaliP34678.
SMRiP34678. Positions 110-611.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini478 – 607130Ricin B-type lectin
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 279111Catalytic subdomain A
Add
BLAST
Regioni339 – 40163Catalytic subdomain B
Add
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00750000117385.
HOGENOMiHOG000038227.
InParanoidiP34678.
KOiK00710.
OMAiCRAVIAT.
OrthoDBiEOG7J9VP2.
PhylomeDBiP34678.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34678-1 [UniParc]FASTAAdd to Basket

« Hide

MLSVGGGRSA VCRAVIATSI VWLLIDVVIL FYYLDPSTSQ QQPFPEDNRI    50
LNRARRIEPL PPAAQHDSDP DAHPIQPEKQ EKQVYPVDKE TANQLRKLME 100
TQAFGPGYHG QGGTGVTVPE DKKTIKEKRF LENQFNVVAS EMISVNRTLP 150
DYRSDACRTS GNNLKTAGMP KTSIIIVFHN EAWTTLLRTL HSVINRSPRH 200
LLEEIILVDD KSDRDYLVKP LDSYIKMFPI PIHLVHLENR SGLIRARLTG 250
SEMAKGKILL FLDAHVEVTD GWLEPLVSRV AEDRKRVVAP IIDVISDDTF 300
EYVTASETTW GGFNWHLNFR WYAVPKRELN RRGSDRSMPI QTPTIAGGLF 350
AIDKQFFYDI GSYDEGMQVW GGENLEISFR VWMCGGSLEI HPCSRVGHVF 400
RKQTPYTFPG GTAKVIHHNA ARTAEVWMDE YKAFFYKMVP AARNVEAGDV 450
SERKKLRETL QCKSFKWYLE NIYPEAPLPA DFRSLGAIVN RFTEKCVDTN 500
GKKDGQAPGI QACHGAGGNQ AWSLTGKGEI RSDDLCLSSG HVYQIGSELK 550
LERCSVSKIN VKHVFVFDDQ AGTLLHKKTG KCVTGADQRV TLDECGLGRK 600
DQMWQLEGYQ SP 612
Length:612
Mass (Da):68,912
Last modified:May 27, 2002 - v2
Checksum:i3031C2FE933F9858
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031833 mRNA. Translation: AAC13669.1.
FO080277 Genomic DNA. Translation: CCD62541.1.
PIRiA88515.
T42243.
RefSeqiNP_498722.1. NM_066321.5.
UniGeneiCel.17588.

Genome annotation databases

EnsemblMetazoaiZK688.8; ZK688.8; WBGene00001628.
GeneIDi176112.
KEGGicel:CELE_ZK688.8.
UCSCiZK688.8.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031833 mRNA. Translation: AAC13669.1 .
FO080277 Genomic DNA. Translation: CCD62541.1 .
PIRi A88515.
T42243.
RefSeqi NP_498722.1. NM_066321.5.
UniGenei Cel.17588.

3D structure databases

ProteinModelPortali P34678.
SMRi P34678. Positions 110-611.
ModBasei Search...

Protein-protein interaction databases

STRINGi 6239.ZK688.8.1.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Proteomic databases

PaxDbi P34678.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZK688.8 ; ZK688.8 ; WBGene00001628 .
GeneIDi 176112.
KEGGi cel:CELE_ZK688.8.
UCSCi ZK688.8.1. c. elegans.

Organism-specific databases

CTDi 176112.
WormBasei ZK688.8 ; CE29649 ; WBGene00001628 ; gly-3.

Phylogenomic databases

eggNOGi NOG239675.
GeneTreei ENSGT00750000117385.
HOGENOMi HOG000038227.
InParanoidi P34678.
KOi K00710.
OMAi CRAVIAT.
OrthoDBi EOG7J9VP2.
PhylomeDBi P34678.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 891164.
PROi P34678.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans."
    Hagen F.K., Nehrke K.
    J. Biol. Chem. 273:8268-8277(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY.
    Strain: Bristol N2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiGALT3_CAEEL
AccessioniPrimary (citable) accession number: P34678
Secondary accession number(s): Q9U003
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 27, 2002
Last modified: June 11, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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