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P34678

- GALT3_CAEEL

UniProt

P34678 - GALT3_CAEEL

Protein

Polypeptide N-acetylgalactosaminyltransferase 3

Gene

gly-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei210 – 2101SubstrateBy similarity
    Binding sitei240 – 2401SubstrateBy similarity
    Metal bindingi263 – 2631ManganeseBy similarity
    Metal bindingi265 – 2651ManganeseBy similarity
    Binding sitei370 – 3701SubstrateBy similarity
    Metal bindingi398 – 3981ManganeseBy similarity
    Binding sitei401 – 4011SubstrateBy similarity
    Binding sitei406 – 4061SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: WormBase

    GO - Biological processi

    1. protein O-linked glycosylation via threonine Source: WormBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 3 (EC:2.4.1.41)
    Alternative name(s):
    GalNAc-T1
    Protein-UDP acetylgalactosaminyltransferase 3
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3
    Short name:
    pp-GaNTase 3
    Gene namesi
    Name:gly-3
    ORF Names:ZK688.8
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiZK688.8; CE29649; WBGene00001628; gly-3.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 612612Polypeptide N-acetylgalactosaminyltransferase 3PRO_0000059146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi157 ↔ 393PROSITE-ProRule annotation
    Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi384 ↔ 462PROSITE-ProRule annotation
    Disulfide bondi496 ↔ 513PROSITE-ProRule annotation
    Disulfide bondi536 ↔ 554PROSITE-ProRule annotation
    Disulfide bondi582 ↔ 595PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP34678.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.ZK688.8.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP34678.
    SMRiP34678. Positions 110-611.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1313CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini35 – 612578LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini478 – 607130Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni169 – 279111Catalytic subdomain AAdd
    BLAST
    Regioni339 – 40163Catalytic subdomain BAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG239675.
    GeneTreeiENSGT00750000117385.
    HOGENOMiHOG000038227.
    InParanoidiP34678.
    KOiK00710.
    OMAiCRAVIAT.
    OrthoDBiEOG7J9VP2.
    PhylomeDBiP34678.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34678-1 [UniParc]FASTAAdd to Basket

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    MLSVGGGRSA VCRAVIATSI VWLLIDVVIL FYYLDPSTSQ QQPFPEDNRI    50
    LNRARRIEPL PPAAQHDSDP DAHPIQPEKQ EKQVYPVDKE TANQLRKLME 100
    TQAFGPGYHG QGGTGVTVPE DKKTIKEKRF LENQFNVVAS EMISVNRTLP 150
    DYRSDACRTS GNNLKTAGMP KTSIIIVFHN EAWTTLLRTL HSVINRSPRH 200
    LLEEIILVDD KSDRDYLVKP LDSYIKMFPI PIHLVHLENR SGLIRARLTG 250
    SEMAKGKILL FLDAHVEVTD GWLEPLVSRV AEDRKRVVAP IIDVISDDTF 300
    EYVTASETTW GGFNWHLNFR WYAVPKRELN RRGSDRSMPI QTPTIAGGLF 350
    AIDKQFFYDI GSYDEGMQVW GGENLEISFR VWMCGGSLEI HPCSRVGHVF 400
    RKQTPYTFPG GTAKVIHHNA ARTAEVWMDE YKAFFYKMVP AARNVEAGDV 450
    SERKKLRETL QCKSFKWYLE NIYPEAPLPA DFRSLGAIVN RFTEKCVDTN 500
    GKKDGQAPGI QACHGAGGNQ AWSLTGKGEI RSDDLCLSSG HVYQIGSELK 550
    LERCSVSKIN VKHVFVFDDQ AGTLLHKKTG KCVTGADQRV TLDECGLGRK 600
    DQMWQLEGYQ SP 612
    Length:612
    Mass (Da):68,912
    Last modified:May 27, 2002 - v2
    Checksum:i3031C2FE933F9858
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031833 mRNA. Translation: AAC13669.1.
    FO080277 Genomic DNA. Translation: CCD62541.1.
    PIRiA88515.
    T42243.
    RefSeqiNP_498722.1. NM_066321.5.
    UniGeneiCel.17588.

    Genome annotation databases

    EnsemblMetazoaiZK688.8; ZK688.8; WBGene00001628.
    GeneIDi176112.
    KEGGicel:CELE_ZK688.8.
    UCSCiZK688.8.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031833 mRNA. Translation: AAC13669.1 .
    FO080277 Genomic DNA. Translation: CCD62541.1 .
    PIRi A88515.
    T42243.
    RefSeqi NP_498722.1. NM_066321.5.
    UniGenei Cel.17588.

    3D structure databases

    ProteinModelPortali P34678.
    SMRi P34678. Positions 110-611.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.ZK688.8.1.

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Proteomic databases

    PaxDbi P34678.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZK688.8 ; ZK688.8 ; WBGene00001628 .
    GeneIDi 176112.
    KEGGi cel:CELE_ZK688.8.
    UCSCi ZK688.8.1. c. elegans.

    Organism-specific databases

    CTDi 176112.
    WormBasei ZK688.8 ; CE29649 ; WBGene00001628 ; gly-3.

    Phylogenomic databases

    eggNOGi NOG239675.
    GeneTreei ENSGT00750000117385.
    HOGENOMi HOG000038227.
    InParanoidi P34678.
    KOi K00710.
    OMAi CRAVIAT.
    OrthoDBi EOG7J9VP2.
    PhylomeDBi P34678.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 891164.
    PROi P34678.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans."
      Hagen F.K., Nehrke K.
      J. Biol. Chem. 273:8268-8277(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY.
      Strain: Bristol N2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGALT3_CAEEL
    AccessioniPrimary (citable) accession number: P34678
    Secondary accession number(s): Q9U003
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3