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Protein

Calnexin

Gene

cnx-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins (By similarity). Required for embryogenesis and larval development under heat and ER stress conditions. May be important for germ cell development. Involved in neuronal necrotic cell death.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Calcium; via carbonyl oxygenBy similarity1
Binding sitei156CarbohydrateBy similarity1
Binding sitei158CarbohydrateBy similarity1
Binding sitei177CarbohydrateBy similarity1
Binding sitei208CarbohydrateBy similarity1
Metal bindingi428CalciumBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: WormBase
  • carbohydrate binding Source: UniProtKB-KW
  • unfolded protein binding Source: InterPro

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • protein folding Source: InterPro
  • response to heat Source: WormBase

Keywordsi

Molecular functionChaperone, Developmental protein
Biological processStress response
LigandCalcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-901042 Calnexin/calreticulin cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin
Short name:
CeCNX-1
Gene namesi
Name:cnx-1
ORF Names:ZK632.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK632.6a ; CE00423 ; WBGene00000567 ; cnx-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei481 – 501HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced fertility and slowed development. Temperature-sensitive reproduction defects.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi203N → Q: Impairs its biological function; when associated with Q-571. 1 Publication1
Mutagenesisi571N → Q: Impairs its biological function; when associated with Q-203. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000420722 – 619CalnexinAdd BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi152 ↔ 186By similarity
Glycosylationi203N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi352 ↔ 358By similarity
Glycosylationi571N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

Glycosylation is important for its biological activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP34652
PaxDbiP34652
PeptideAtlasiP34652
PRIDEiP34652

PTM databases

iPTMnetiP34652

Expressioni

Tissue specificityi

Expressed ubiquitously in every blastomere of the embryo up to the gastrulation stage. Expression becomes gradually restricted to the head and tail regions at the comma stage during embryogenesis. During postembryonic development, expressed prominently in the H-shaped excretory cell, in the neurons of head (including ASK and ADL) and tail (including PHA and PHB), in the dorsal and ventral nerve cords, and in the spermatheca. Expressed in the spicules of the male tail (at protein level).1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Inductioni

By stress conditions, such as higher temperature, EGTA, DTT or tunicamycin (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00000567
ExpressionAtlasiP34652 baseline and differential

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi41584, 2 interactors
DIPiDIP-25990N
IntActiP34652, 2 interactors
STRINGi6239.ZK632.6.1

Structurei

3D structure databases

ProteinModelPortaliP34652
SMRiP34652
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati270 – 2821-1Add BLAST13
Repeati287 – 2991-2Add BLAST13
Repeati306 – 3181-3Add BLAST13
Repeati325 – 3371-4Add BLAST13
Repeati340 – 3502-1Add BLAST11
Repeati359 – 3692-2Add BLAST11
Repeati373 – 3832-3Add BLAST11
Repeati387 – 3972-4Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 401P domain (Extended arm)By similarityAdd BLAST134
Regioni270 – 3374 X approximate repeatsAdd BLAST68
Regioni340 – 3974 X approximate repeatsAdd BLAST58

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675 Eukaryota
ENOG410XP7T LUCA
GeneTreeiENSGT00430000030841
HOGENOMiHOG000192436
InParanoidiP34652
KOiK08054
OMAiNHKNPKT
OrthoDBiEOG091G04SS
PhylomeDBiP34652

Family and domain databases

Gene3Di2.10.250.10, 1 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
PS00805 CALRETICULIN_REPEAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNRKWMYIF IQFLLVSSIR SDDDVFEDDE EEVTKGSDDK EEFVPSLFVA
60 70 80 90 100
PKLSDKSTPN FFDYFPVGSK IGLTWIKSLA KKDDVDSDIA KYNGEWSIGA
110 120 130 140 150
PTKVSIEGDL GLIVKTKARH HAIAAKLNTP FAFDANTFVV QYDIKFEEGQ
160 170 180 190 200
ECGGGYLKLL SEGAEKDLAN FQDKTAYTIM FGPDKCGATG KVHLIFRYKN
210 220 230 240 250
PINGTISEYH ANQPTTIGST YWDDHNTHLF TLVVKPTGEY SVSVDGKSLY
260 270 280 290 300
YGNMMSDVTP ALTPPKQIFD ETDLKPVDWD ERENIEDESA VKPDDWDENE
310 320 330 340 350
PQSVVDEAAT KPYDWNEEEN ELIADPEAKK PQDWDEDMDG SWEAPLIDNP
360 370 380 390 400
ACKGLSGCGT WKAPTIKNPK YKGKWIRPKI SNPAFKGKWT ARLIDNPNYF
410 420 430 440 450
EPKPFAGLAP ITAVGIEMWT MSENILFDNI LITSSEEDSS DVAKQTFYVK
460 470 480 490 500
QKEEYRLAAA TGNGNGFFQQ IIDATNEKPW LWAVYILCVL LPLVAIGVFC
510 520 530 540 550
FGKQSKPTPN FAKKSDAYSA DDDRVPNLVD DDEEEIIGDE EDDVNQPGPS
560 570 580 590 600
GSQSNPEPQD EEENAEQQSA NSSQSSAAEE EDDEHVVPEN EPVKPTEEFA
610
KKSPKNTGGA KRRTARRGD
Length:619
Mass (Da):69,208
Last modified:February 1, 1994 - v1
Checksum:i08903CE519A75B88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22181 Genomic DNA Translation: CAA80183.1
PIRiS40938
RefSeqiNP_499176.1, NM_066775.4
UniGeneiCel.17175

Genome annotation databases

EnsemblMetazoaiZK632.6a; ZK632.6a; WBGene00000567
GeneIDi176390
KEGGicel:CELE_ZK632.6
UCSCiZK632.6 c. elegans

Similar proteinsi

Entry informationi

Entry nameiCALX_CAEEL
AccessioniPrimary (citable) accession number: P34652
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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