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Protein

Calnexin

Gene

cnx-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins (By similarity). Required for embryogenesis and larval development under heat and ER stress conditions. May be important for germ cell development. Involved in neuronal necrotic cell death.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Calcium; via carbonyl oxygenBy similarity
Binding sitei156 – 1561CarbohydrateBy similarity
Binding sitei158 – 1581CarbohydrateBy similarity
Binding sitei177 – 1771CarbohydrateBy similarity
Binding sitei208 – 2081CarbohydrateBy similarity
Metal bindingi428 – 4281CalciumBy similarity

GO - Molecular functioni

  • calcium ion binding Source: WormBase
  • carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • protein folding Source: InterPro
  • response to heat Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Developmental protein

Keywords - Biological processi

Stress response

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin
Short name:
CeCNX-1
Gene namesi
Name:cnx-1
ORF Names:ZK632.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK632.6a; CE00423; WBGene00000567; cnx-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei481 – 50121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • integral component of endoplasmic reticulum membrane Source: WormBase
  • perinuclear region of cytoplasm Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced fertility and slowed development. Temperature-sensitive reproduction defects.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi203 – 2031N → Q: Impairs its biological function; when associated with Q-571. 1 Publication
Mutagenesisi571 – 5711N → Q: Impairs its biological function; when associated with Q-203. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 619598CalnexinPRO_0000004207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi152 ↔ 186By similarity
Glycosylationi203 – 2031N-linked (GlcNAc...)1 Publication
Disulfide bondi352 ↔ 358By similarity
Glycosylationi571 – 5711N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Glycosylation is important for its biological activity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP34652.
PaxDbiP34652.

Expressioni

Tissue specificityi

Expressed ubiquitously in every blastomere of the embryo up to the gastrulation stage. Expression becomes gradually restricted to the head and tail regions at the comma stage during embryogenesis. During postembryonic development, expressed prominently in the H-shaped excretory cell, in the neurons of head (including ASK and ADL) and tail (including PHA and PHB), in the dorsal and ventral nerve cords, and in the spermatheca. Expressed in the spicules of the male tail (at protein level).1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Inductioni

By stress conditions, such as higher temperature, EGTA, DTT or tunicamycin (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiP34652. baseline.

Interactioni

Protein-protein interaction databases

BioGridi41584. 2 interactions.
DIPiDIP-25990N.
IntActiP34652. 2 interactions.
MINTiMINT-1050231.
STRINGi6239.ZK632.6.1.

Structurei

3D structure databases

ProteinModelPortaliP34652.
SMRiP34652. Positions 61-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati270 – 282131-1Add
BLAST
Repeati287 – 299131-2Add
BLAST
Repeati306 – 318131-3Add
BLAST
Repeati325 – 337131-4Add
BLAST
Repeati340 – 350112-1Add
BLAST
Repeati359 – 369112-2Add
BLAST
Repeati373 – 383112-3Add
BLAST
Repeati387 – 397112-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 401134P domain (Extended arm)By similarityAdd
BLAST
Regioni270 – 337684 X approximate repeatsAdd
BLAST
Regioni340 – 397584 X approximate repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
InParanoidiP34652.
KOiK08054.
OMAiNTHLFTL.
PhylomeDBiP34652.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNRKWMYIF IQFLLVSSIR SDDDVFEDDE EEVTKGSDDK EEFVPSLFVA
60 70 80 90 100
PKLSDKSTPN FFDYFPVGSK IGLTWIKSLA KKDDVDSDIA KYNGEWSIGA
110 120 130 140 150
PTKVSIEGDL GLIVKTKARH HAIAAKLNTP FAFDANTFVV QYDIKFEEGQ
160 170 180 190 200
ECGGGYLKLL SEGAEKDLAN FQDKTAYTIM FGPDKCGATG KVHLIFRYKN
210 220 230 240 250
PINGTISEYH ANQPTTIGST YWDDHNTHLF TLVVKPTGEY SVSVDGKSLY
260 270 280 290 300
YGNMMSDVTP ALTPPKQIFD ETDLKPVDWD ERENIEDESA VKPDDWDENE
310 320 330 340 350
PQSVVDEAAT KPYDWNEEEN ELIADPEAKK PQDWDEDMDG SWEAPLIDNP
360 370 380 390 400
ACKGLSGCGT WKAPTIKNPK YKGKWIRPKI SNPAFKGKWT ARLIDNPNYF
410 420 430 440 450
EPKPFAGLAP ITAVGIEMWT MSENILFDNI LITSSEEDSS DVAKQTFYVK
460 470 480 490 500
QKEEYRLAAA TGNGNGFFQQ IIDATNEKPW LWAVYILCVL LPLVAIGVFC
510 520 530 540 550
FGKQSKPTPN FAKKSDAYSA DDDRVPNLVD DDEEEIIGDE EDDVNQPGPS
560 570 580 590 600
GSQSNPEPQD EEENAEQQSA NSSQSSAAEE EDDEHVVPEN EPVKPTEEFA
610
KKSPKNTGGA KRRTARRGD
Length:619
Mass (Da):69,208
Last modified:February 1, 1994 - v1
Checksum:i08903CE519A75B88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22181 Genomic DNA. Translation: CAA80183.1.
PIRiS40938.
RefSeqiNP_499176.1. NM_066775.4.
UniGeneiCel.17175.

Genome annotation databases

EnsemblMetazoaiZK632.6a; ZK632.6a; WBGene00000567.
GeneIDi176390.
KEGGicel:CELE_ZK632.6.
UCSCiZK632.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22181 Genomic DNA. Translation: CAA80183.1.
PIRiS40938.
RefSeqiNP_499176.1. NM_066775.4.
UniGeneiCel.17175.

3D structure databases

ProteinModelPortaliP34652.
SMRiP34652. Positions 61-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41584. 2 interactions.
DIPiDIP-25990N.
IntActiP34652. 2 interactions.
MINTiMINT-1050231.
STRINGi6239.ZK632.6.1.

Proteomic databases

EPDiP34652.
PaxDbiP34652.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK632.6a; ZK632.6a; WBGene00000567.
GeneIDi176390.
KEGGicel:CELE_ZK632.6.
UCSCiZK632.6. c. elegans.

Organism-specific databases

CTDi176390.
WormBaseiZK632.6a; CE00423; WBGene00000567; cnx-1.

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
InParanoidiP34652.
KOiK08054.
OMAiNTHLFTL.
PhylomeDBiP34652.

Enzyme and pathway databases

ReactomeiR-CEL-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

NextBioi892370.
PROiP34652.

Gene expression databases

ExpressionAtlasiP34652. baseline.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Caenorhabditis elegans calnexin is N-glycosylated and required for stress response."
    Lee W., Lee T.H., Park B.J., Chang J.W., Yu J.R., Koo H.S., Park H., Yoo Y.J., Ahnn J.
    Biochem. Biophys. Res. Commun. 338:1018-1030(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE, GLYCOSYLATION AT ASN-203 AND ASN-571, MUTAGENESIS OF ASN-203 AND ASN-571.
    Strain: Bristol N2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Necrotic cell death in C. elegans requires the function of calreticulin and regulators of Ca(2+) release from the endoplasmic reticulum."
    Xu K., Tavernarakis N., Driscoll M.
    Neuron 31:957-971(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCALX_CAEEL
AccessioniPrimary (citable) accession number: P34652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.