P34650 (MPI_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannose-6-phosphate isomerase
EC=5.3.1.8

Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI

Gene names

ORF Names:

ZK632.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Probable mannose-6-phosphate isomerase		PRO_0000194233
Sites
	Active site	278	1	By similarity
	Metal binding	99	1	Zinc By similarity
	Metal binding	101	1	Zinc By similarity
	Metal binding	126	1	Zinc By similarity
	Metal binding	259	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34650 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: 1C7DEDEE85DB8459	FASTA	416	46,384
	10 20 30 40 50 60 MLLKLKCTVN NYAWGPKGNS SMAGSLALDG GHIPNLDKDK PYAEFWVGTH ANGPAHVIEK 70 80 90 100 110 120 DIALKQLLAT SPELQGKHEK GNLSFLFKVL SVLGPLSIQI HPTKEQGKLL HATDPKNYPD 130 140 150 160 170 180 DNHKPEIAIA LTEFELLSGF RQHSQISEYL KLYSEIQELL TEEEKSQIDS LGSYGESSAQ 190 200 210 220 230 240 VLKKIFSRIW RTPKEKLQIV VDKLARRIQG HENKTALDEI IVYLFTLYPG DVGVFAPIFL 250 260 270 280 290 300 NYFKLQPGEA TFLEPNMPHA YLKGDCVECM ADSDNTIRAG LTPKYIDVES LVEMLNYDET 310 320 330 340 350 360 LLPKYIPNEL DDGSLLFTPR GIDEFWVQEV KGPAGSIYQL PYSESCSVLT VLYGTATVTL 370 380 390 400 410 GDASQVLNRG EVVFIGATHD AERPKINISD DFLAFRAFTP SPRALESLSN KRLIID

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22181 Genomic DNA. Translation: CAA80181.3.
PIR	S40936.
RefSeq	NP_499174.3. NM_066773.3.
UniGene	Cel.9546.
3D structure databases
ProteinModelPortal	P34650.
SMR	P34650. Positions 2-401.
ModBase	Search...
Protein-protein interaction databases
STRING	P34650.
Proteomic databases
PRIDE	P34650.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZK632.4; ZK632.4; ZK632.4.
GeneID	176388.
KEGG	cel:ZK632.4.
NMPDR	fig|6239.3.peg.11275.
UCSC	ZK632.4. c. elegans.
Organism-specific databases
CTD	176388.
WormBase	ZK632.4; CE36417; WBGene00014013.
Phylogenomic databases
eggNOG	meNOG06971.
GeneTree	EMGT00050000017218.
HOGENOM	HBG590632.
InParanoid	P34650.
OMA	YGTATVT.
PhylomeDB	P34650.
Gene expression databases
ArrayExpress	P34650.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 3 hits.
KO	K01809.
PANTHER	PTHR10309. PTHR10309. 1 hit.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR00218. ManA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	892362.

Entry information

Entry name

MPI_CAEEL

Accession

Primary (citable) accession number: P34650

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	August 16, 2004
Last modified:	November 16, 2011
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project