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Protein

Leucine aminopeptidase 1

Gene

lap-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably acts as a digestive enzyme.1 Publication

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi252Zinc 2By similarity1
Metal bindingi257Zinc 1By similarity1
Metal bindingi257Zinc 2By similarity1
Active sitei264Sequence analysis1
Metal bindingi275Zinc 2By similarity1
Metal bindingi334Zinc 1By similarity1
Metal bindingi336Zinc 1By similarity1
Metal bindingi336Zinc 2By similarity1
Active sitei338Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • regulation of growth rate Source: WormBase
  • regulation of oviposition Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM17.A05.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine aminopeptidase 1 (EC:3.4.11.1)
Gene namesi
Name:lap-1
ORF Names:ZK353.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK353.6; CE00390; WBGene00002249; lap-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001658311 – 491Leucine aminopeptidase 1Add BLAST491

Proteomic databases

EPDiP34629.
PaxDbiP34629.
PeptideAtlasiP34629.
PRIDEiP34629.

Expressioni

Tissue specificityi

Expressed in the buccal cavity, pharynx, anterior gut and rectum.1 Publication

Gene expression databases

BgeeiWBGene00002249.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-332157,EBI-332157
lbp-6O018122EBI-332157,EBI-327961

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi41389. 7 interactors.
DIPiDIP-27312N.
IntActiP34629. 3 interactors.
MINTiMINT-1056525.
STRINGi6239.ZK353.6.1.

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Beta strandi18 – 25Combined sources8
Helixi26 – 31Combined sources6
Helixi36 – 42Combined sources7
Turni43 – 45Combined sources3
Helixi49 – 58Combined sources10
Beta strandi61 – 77Combined sources17
Helixi90 – 97Combined sources8
Beta strandi108 – 114Combined sources7
Helixi117 – 119Combined sources3
Helixi120 – 128Combined sources9
Beta strandi145 – 151Combined sources7
Helixi157 – 178Combined sources22
Turni181 – 183Combined sources3
Helixi186 – 199Combined sources14
Beta strandi203 – 208Combined sources6
Helixi210 – 215Combined sources6
Helixi218 – 224Combined sources7
Beta strandi227 – 229Combined sources3
Beta strandi232 – 238Combined sources7
Beta strandi246 – 257Combined sources12
Turni266 – 268Combined sources3
Helixi272 – 276Combined sources5
Helixi277 – 290Combined sources14
Turni291 – 293Combined sources3
Beta strandi296 – 307Combined sources12
Beta strandi319 – 321Combined sources3
Beta strandi327 – 329Combined sources3
Helixi337 – 350Combined sources14
Beta strandi355 – 361Combined sources7
Helixi367 – 371Combined sources5
Turni372 – 374Combined sources3
Beta strandi375 – 381Combined sources7
Helixi383 – 396Combined sources14
Beta strandi400 – 402Combined sources3
Helixi407 – 410Combined sources4
Helixi411 – 414Combined sources4
Beta strandi417 – 423Combined sources7
Helixi433 – 442Combined sources10
Turni443 – 445Combined sources3
Helixi446 – 448Combined sources3
Beta strandi451 – 457Combined sources7
Turni459 – 462Combined sources4
Helixi473 – 480Combined sources8
Helixi481 – 483Combined sources3
Helixi487 – 489Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HB6X-ray2.00A/B1-491[»]
2HC9X-ray1.85A1-491[»]
ProteinModelPortaliP34629.
SMRiP34629.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34629.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243131.
InParanoidiP34629.
KOiK09611.
OMAiMDGPPSA.
OrthoDBiEOG091G0A99.
PhylomeDBiP34629.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVLVRNGI QAVGDGLTSL IIVGKKSVLK NVTFEGKFKE VAQKFVTDGD
60 70 80 90 100
SWNSMISRIP ASGRHPLHYE LAHLITVPDA SSRGNTPTNA HSIYKELKPI
110 120 130 140 150
NYPEDTKNVH FVLFAEYPDV LSHVAAIART FCKFSMKTSG IRELNVNIDV
160 170 180 190 200
VCDKLTNEDA VFLTDLSESV RETARLIDTP ANILTTDALV DEAVKVGNAT
210 220 230 240 250
GSKITVIRGE ELLKAGFGGI YHVGKAGPTP PAFVVLSHEV PGSTEHIALV
260 270 280 290 300
GKGVVYDTGG LQIKTKTGMP NMKRDMGGAA GMLEAYSALV KHGFSQTLHA
310 320 330 340 350
CLCIVENNVS PIANKPDDII KMLSGKTVEI NNTDAEGRLI LADGVFYAKE
360 370 380 390 400
TLKATTIFDM ATLTGAQAWL SGRLHGAAMT NDEQLENEII KAGKASGDLV
410 420 430 440 450
APMLFAPDLF FGDLKSSIAD MKNSNLGKMD GPPSAVAGLF IGAHIGFGEG
460 470 480 490
LRWLHLDIAA PAEVGDRGTG YGPALFSTLL GKYTSVPMLK Q
Length:491
Mass (Da):52,449
Last modified:February 1, 1994 - v1
Checksum:iAECC10758180D1EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081668 Genomic DNA. Translation: CCD73205.1.
PIRiS44657.
RefSeqiNP_498854.1. NM_066453.3.
UniGeneiCel.5920.

Genome annotation databases

EnsemblMetazoaiZK353.6; ZK353.6; WBGene00002249.
GeneIDi176185.
KEGGicel:CELE_ZK353.6.
UCSCiZK353.6.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081668 Genomic DNA. Translation: CCD73205.1.
PIRiS44657.
RefSeqiNP_498854.1. NM_066453.3.
UniGeneiCel.5920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HB6X-ray2.00A/B1-491[»]
2HC9X-ray1.85A1-491[»]
ProteinModelPortaliP34629.
SMRiP34629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41389. 7 interactors.
DIPiDIP-27312N.
IntActiP34629. 3 interactors.
MINTiMINT-1056525.
STRINGi6239.ZK353.6.1.

Protein family/group databases

MEROPSiM17.A05.

Proteomic databases

EPDiP34629.
PaxDbiP34629.
PeptideAtlasiP34629.
PRIDEiP34629.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK353.6; ZK353.6; WBGene00002249.
GeneIDi176185.
KEGGicel:CELE_ZK353.6.
UCSCiZK353.6.1. c. elegans.

Organism-specific databases

CTDi176185.
WormBaseiZK353.6; CE00390; WBGene00002249; lap-1.

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243131.
InParanoidiP34629.
KOiK09611.
OMAiMDGPPSA.
OrthoDBiEOG091G0A99.
PhylomeDBiP34629.

Miscellaneous databases

EvolutionaryTraceiP34629.
PROiP34629.

Gene expression databases

BgeeiWBGene00002249.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPL_CAEEL
AccessioniPrimary (citable) accession number: P34629
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.