Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine aminopeptidase 1

Gene

lap-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably acts as a digestive enzyme.1 Publication

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521Zinc 2By similarity
Metal bindingi257 – 2571Zinc 1By similarity
Metal bindingi257 – 2571Zinc 2By similarity
Active sitei264 – 2641Sequence analysis
Metal bindingi275 – 2751Zinc 2By similarity
Metal bindingi334 – 3341Zinc 1By similarity
Metal bindingi336 – 3361Zinc 1By similarity
Metal bindingi336 – 3361Zinc 2By similarity
Active sitei338 – 3381Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • regulation of growth rate Source: WormBase
  • regulation of oviposition Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM17.A05.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine aminopeptidase 1 (EC:3.4.11.1)
Gene namesi
Name:lap-1
ORF Names:ZK353.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK353.6; CE00390; WBGene00002249; lap-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Leucine aminopeptidase 1PRO_0000165831Add
BLAST

Proteomic databases

EPDiP34629.
PaxDbiP34629.

Expressioni

Tissue specificityi

Expressed in the buccal cavity, pharynx, anterior gut and rectum.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-332157,EBI-332157
lbp-6O018122EBI-332157,EBI-327961

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi41389. 7 interactions.
DIPiDIP-27312N.
IntActiP34629. 3 interactions.
MINTiMINT-1056525.
STRINGi6239.ZK353.6.1.

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Beta strandi18 – 258Combined sources
Helixi26 – 316Combined sources
Helixi36 – 427Combined sources
Turni43 – 453Combined sources
Helixi49 – 5810Combined sources
Beta strandi61 – 7717Combined sources
Helixi90 – 978Combined sources
Beta strandi108 – 1147Combined sources
Helixi117 – 1193Combined sources
Helixi120 – 1289Combined sources
Beta strandi145 – 1517Combined sources
Helixi157 – 17822Combined sources
Turni181 – 1833Combined sources
Helixi186 – 19914Combined sources
Beta strandi203 – 2086Combined sources
Helixi210 – 2156Combined sources
Helixi218 – 2247Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi232 – 2387Combined sources
Beta strandi246 – 25712Combined sources
Turni266 – 2683Combined sources
Helixi272 – 2765Combined sources
Helixi277 – 29014Combined sources
Turni291 – 2933Combined sources
Beta strandi296 – 30712Combined sources
Beta strandi319 – 3213Combined sources
Beta strandi327 – 3293Combined sources
Helixi337 – 35014Combined sources
Beta strandi355 – 3617Combined sources
Helixi367 – 3715Combined sources
Turni372 – 3743Combined sources
Beta strandi375 – 3817Combined sources
Helixi383 – 39614Combined sources
Beta strandi400 – 4023Combined sources
Helixi407 – 4104Combined sources
Helixi411 – 4144Combined sources
Beta strandi417 – 4237Combined sources
Helixi433 – 44210Combined sources
Turni443 – 4453Combined sources
Helixi446 – 4483Combined sources
Beta strandi451 – 4577Combined sources
Turni459 – 4624Combined sources
Helixi473 – 4808Combined sources
Helixi481 – 4833Combined sources
Helixi487 – 4893Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HB6X-ray2.00A/B1-491[»]
2HC9X-ray1.85A1-491[»]
ProteinModelPortaliP34629.
SMRiP34629. Positions 2-490.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP34629.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243131.
InParanoidiP34629.
KOiK09611.
OMAiMDGPPSA.
OrthoDBiEOG7966GR.
PhylomeDBiP34629.

Family and domain databases

InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVLVRNGI QAVGDGLTSL IIVGKKSVLK NVTFEGKFKE VAQKFVTDGD
60 70 80 90 100
SWNSMISRIP ASGRHPLHYE LAHLITVPDA SSRGNTPTNA HSIYKELKPI
110 120 130 140 150
NYPEDTKNVH FVLFAEYPDV LSHVAAIART FCKFSMKTSG IRELNVNIDV
160 170 180 190 200
VCDKLTNEDA VFLTDLSESV RETARLIDTP ANILTTDALV DEAVKVGNAT
210 220 230 240 250
GSKITVIRGE ELLKAGFGGI YHVGKAGPTP PAFVVLSHEV PGSTEHIALV
260 270 280 290 300
GKGVVYDTGG LQIKTKTGMP NMKRDMGGAA GMLEAYSALV KHGFSQTLHA
310 320 330 340 350
CLCIVENNVS PIANKPDDII KMLSGKTVEI NNTDAEGRLI LADGVFYAKE
360 370 380 390 400
TLKATTIFDM ATLTGAQAWL SGRLHGAAMT NDEQLENEII KAGKASGDLV
410 420 430 440 450
APMLFAPDLF FGDLKSSIAD MKNSNLGKMD GPPSAVAGLF IGAHIGFGEG
460 470 480 490
LRWLHLDIAA PAEVGDRGTG YGPALFSTLL GKYTSVPMLK Q
Length:491
Mass (Da):52,449
Last modified:February 1, 1994 - v1
Checksum:iAECC10758180D1EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081668 Genomic DNA. Translation: CCD73205.1.
PIRiS44657.
RefSeqiNP_498854.1. NM_066453.3.
UniGeneiCel.5920.

Genome annotation databases

EnsemblMetazoaiZK353.6; ZK353.6; WBGene00002249.
GeneIDi176185.
KEGGicel:CELE_ZK353.6.
UCSCiZK353.6.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081668 Genomic DNA. Translation: CCD73205.1.
PIRiS44657.
RefSeqiNP_498854.1. NM_066453.3.
UniGeneiCel.5920.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HB6X-ray2.00A/B1-491[»]
2HC9X-ray1.85A1-491[»]
ProteinModelPortaliP34629.
SMRiP34629. Positions 2-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41389. 7 interactions.
DIPiDIP-27312N.
IntActiP34629. 3 interactions.
MINTiMINT-1056525.
STRINGi6239.ZK353.6.1.

Protein family/group databases

MEROPSiM17.A05.

Proteomic databases

EPDiP34629.
PaxDbiP34629.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK353.6; ZK353.6; WBGene00002249.
GeneIDi176185.
KEGGicel:CELE_ZK353.6.
UCSCiZK353.6.1. c. elegans.

Organism-specific databases

CTDi176185.
WormBaseiZK353.6; CE00390; WBGene00002249; lap-1.

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243131.
InParanoidiP34629.
KOiK09611.
OMAiMDGPPSA.
OrthoDBiEOG7966GR.
PhylomeDBiP34629.

Miscellaneous databases

EvolutionaryTraceiP34629.
PROiP34629.

Family and domain databases

InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Functional analysis of leucine aminopeptidase in Caenorhabditis elegans."
    Joshua G.W.
    Mol. Biochem. Parasitol. 113:223-232(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiAMPL_CAEEL
AccessioniPrimary (citable) accession number: P34629
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.