Reviewed, UniProtKB/Swiss-Prot P34609 (JIP_CAEEL)

Names and origin

Protein names

Recommended name:
    JNK-interacting protein
      Short name=JIP
Alternative name(s):
    JNK MAP kinase scaffold protein
    Uncoordinated protein 16

Gene names

Name:	unc-16
ORF Names:	ZK1098.10

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of synaptic vesicle transport, through interations with the JNK-signaling components and motor proteins. Binds specific components of the JNK signaling pathway namely jnk-1, jkk-1 and sek-1. Associates with components of the motor protein, kinesin-1. Pre-assembled unc-16 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location. Ref.1 Ref.4
Subcellular location	Cytoplasm › perinuclear region. Note: Diffusely localized throughout cell body but intensely localized in regions adjacent to nucleus and at presumptive tips of neural processes. Ref.1 Ref.4
Tissue specificity	Expressed in neurons of the ventral cord, retrovesicular and preanal ganglia and nerve ring, intestinal cells, seam and hypodermal cells, body wall, head muscle and pharynx. Ref.1 Ref.4
Sequence similarities	Belongs to the JIP scaffold family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of JNK cascade Ref.1 Inferred from direct assay. Source: UniProtKB vesicle-mediated transport Ref.4 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	MAP-kinase scaffold activity Ref.1 Inferred from physical interaction. Source: UniProtKB kinesin binding Ref.4 Inferred from mutant phenotype. Source: UniProtKB protein kinase binding Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q18668	1	EBI-315684,EBI-317396
F46G11.1	Q5WRT0	1	EBI-315684,EBI-322655
gei-4	Q9TZ38	1	EBI-315684,EBI-329192
jkk-1	Q9UAH1	1	EBI-315684,EBI-321088
jnk-1	Q8WQG9	1	EBI-315684,EBI-321822
klc-2	P46822	1	EBI-315684,EBI-315578
Y41D4B.18	Q95Y19	1	EBI-315684,EBI-1811923

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform b (identifier: P34609-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform a (identifier: P34609-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.

Note: No experimental confirmation available.

Isoform c (identifier: P34609-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: Missing.
     331-331: G → GNLVDPAEFASAVNDTFIG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1157	1157	JNK-interacting protein		PRO_0000220637
Regions
	Coiled coil	73 – 181	109	Potential
	Coiled coil	319 – 474	156	Potential
	Coiled coil	621 – 645	25	Potential
Natural variations
	Alternative sequence	1 – 36	36	Missing in isoform a and isoform c.		VSP_002781
	Alternative sequence	331	1	G → GNLVDPAEFASAVNDTFIG in isoform c.		VSP_002782

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified November 25, 2002. Version 3. Checksum: 44FA445C96B78A59	FASTA	1,157	130,480
	10 20 30 40 50 60 MACNLSPVNE MADSITSSTP SEIVYGGPGS PDEHRTMSDK VQTMASAIYR ELETMIKVHG 70 80 90 100 110 120 EDGVKTLMPL VVNVLEALDL AYLERDEQTA ELEMLKEDNE QLQTQYEREK ALRKQTEQKY 130 140 150 160 170 180 IEIEDTLIGQ NKELDKKIES LESIMRMLEL KAKNATDHAS RLEEREVEQK LEFDRLHERY 190 200 210 220 230 240 NTLLRTHVDH MERTKYLMGS EKFELMQNMP LPNMQLRNKM GMAASVDASS IRGVSDLISA 250 260 270 280 290 300 HMTQSTTMDV NLANHITNED WQDEFSSDIE PSPRDIPQSS ADALTSPITT KEPTPKREAA 310 320 330 340 350 360 SPKQSEEEEA DETTSVDPKE NNDLLGADLT GMGREVENLI KENSELLDMK NALNIVKNDL 370 380 390 400 410 420 INQVDELNSE NMILRDENLS RQMVSEKMQE QITKHEEEIK TLKQKLMEKE NEQEEDDVPM 430 440 450 460 470 480 AMRKRFTRSE MQRVLMDRNA YKEKLMELEE SIKWTEMQRA KKMQQQQQNV NQKKSGGIWE 490 500 510 520 530 540 FFSSLLGDSV TPPASSRGNR ASSSRGKMTR SVEYIDPDMI SERRAAERRE QYKLVREHVK 550 560 570 580 590 600 KEDGRIEAYG WSLPNVEAEV SSVPIPVCCR PLLDNEPSLK IWCATGVVLR GGRDERGQWI 610 620 630 640 650 660 VGDPIYFAPA SMKKTKTSNH RPELEDEIKR ARNLDARESE LDEWQSSSLV WVVSSNQGKS 670 680 690 700 710 720 LIAVLDANNP NNIIETFPAC DSHLLCIQAV SGVMEGEPEM NEEQSKKYLS GGGKIKDLPE 730 740 750 760 770 780 GLDGTDLGAC EWVELRKMED SEDGVPTYCS NDMKPSPKRT RDFSISEVAP VDSSAPVKED 790 800 810 820 830 840 PLPPPANRPG GRAALPPHIR DAMSKYDGVS GQMSGALPTV WMGGQNQYIY IHSAVTAWKQ 850 860 870 880 890 900 CLRRIKMPDA VLSIVHYKSR IFAALANGTI AIFHRNKHGE WSDEGYHSLR VGSATSSVRS 910 920 930 940 950 960 LCLVSTNIWA TYKNCVVVLD AESLQIVKVF AAHPRKDSQV RNMQWVGAGV WLSIRLDSTL 970 980 990 1000 1010 1020 RLYHAHTYEH LQDVDIEPYV TKMLGTSKLD FSYMRTTALL VSNRRLWIGT GTGVIISVPF 1030 1040 1050 1060 1070 1080 SGQLEKKIET KDSKRPAGPG GLVRVYGATS ENATNDEKTN DDFIPYCNLA HAQLSFHGHK 1090 1100 1110 1120 1130 1140 DSVKFFLGVP GASKNGEDES AEVTLRRMLI MSGGDGYIDF RIGEENEPEL TGQSIRPRDM 1150 SHLIIWEVDA ELPILSK
	Isoform a. Checksum: 1A316F5A561CBB4A	FASTA	1,121	126,747
	10 20 30 40 50 60 MSDKVQTMAS AIYRELETMI KVHGEDGVKT LMPLVVNVLE ALDLAYLERD EQTAELEMLK 70 80 90 100 110 120 EDNEQLQTQY EREKALRKQT EQKYIEIEDT LIGQNKELDK KIESLESIMR MLELKAKNAT 130 140 150 160 170 180 DHASRLEERE VEQKLEFDRL HERYNTLLRT HVDHMERTKY LMGSEKFELM QNMPLPNMQL 190 200 210 220 230 240 RNKMGMAASV DASSIRGVSD LISAHMTQST TMDVNLANHI TNEDWQDEFS SDIEPSPRDI 250 260 270 280 290 300 PQSSADALTS PITTKEPTPK REAASPKQSE EEEADETTSV DPKENNDLLG ADLTGMGREV 310 320 330 340 350 360 ENLIKENSEL LDMKNALNIV KNDLINQVDE LNSENMILRD ENLSRQMVSE KMQEQITKHE 370 380 390 400 410 420 EEIKTLKQKL MEKENEQEED DVPMAMRKRF TRSEMQRVLM DRNAYKEKLM ELEESIKWTE 430 440 450 460 470 480 MQRAKKMQQQ QQNVNQKKSG GIWEFFSSLL GDSVTPPASS RGNRASSSRG KMTRSVEYID 490 500 510 520 530 540 PDMISERRAA ERREQYKLVR EHVKKEDGRI EAYGWSLPNV EAEVSSVPIP VCCRPLLDNE 550 560 570 580 590 600 PSLKIWCATG VVLRGGRDER GQWIVGDPIY FAPASMKKTK TSNHRPELED EIKRARNLDA 610 620 630 640 650 660 RESELDEWQS SSLVWVVSSN QGKSLIAVLD ANNPNNIIET FPACDSHLLC IQAVSGVMEG 670 680 690 700 710 720 EPEMNEEQSK KYLSGGGKIK DLPEGLDGTD LGACEWVELR KMEDSEDGVP TYCSNDMKPS 730 740 750 760 770 780 PKRTRDFSIS EVAPVDSSAP VKEDPLPPPA NRPGGRAALP PHIRDAMSKY DGVSGQMSGA 790 800 810 820 830 840 LPTVWMGGQN QYIYIHSAVT AWKQCLRRIK MPDAVLSIVH YKSRIFAALA NGTIAIFHRN 850 860 870 880 890 900 KHGEWSDEGY HSLRVGSATS SVRSLCLVST NIWATYKNCV VVLDAESLQI VKVFAAHPRK 910 920 930 940 950 960 DSQVRNMQWV GAGVWLSIRL DSTLRLYHAH TYEHLQDVDI EPYVTKMLGT SKLDFSYMRT 970 980 990 1000 1010 1020 TALLVSNRRL WIGTGTGVII SVPFSGQLEK KIETKDSKRP AGPGGLVRVY GATSENATND 1030 1040 1050 1060 1070 1080 EKTNDDFIPY CNLAHAQLSF HGHKDSVKFF LGVPGASKNG EDESAEVTLR RMLIMSGGDG 1090 1100 1110 1120 YIDFRIGEEN EPELTGQSIR PRDMSHLIIW EVDAELPILS K
	Isoform c. Checksum: 65517DA05AF2A2B4	FASTA	1,139	128,609
	10 20 30 40 50 60 MSDKVQTMAS AIYRELETMI KVHGEDGVKT LMPLVVNVLE ALDLAYLERD EQTAELEMLK 70 80 90 100 110 120 EDNEQLQTQY EREKALRKQT EQKYIEIEDT LIGQNKELDK KIESLESIMR MLELKAKNAT 130 140 150 160 170 180 DHASRLEERE VEQKLEFDRL HERYNTLLRT HVDHMERTKY LMGSEKFELM QNMPLPNMQL 190 200 210 220 230 240 RNKMGMAASV DASSIRGVSD LISAHMTQST TMDVNLANHI TNEDWQDEFS SDIEPSPRDI 250 260 270 280 290 300 PQSSADALTS PITTKEPTPK REAASPKQSE EEEADETTSV DPKENNDLLG ADLTGNLVDP 310 320 330 340 350 360 AEFASAVNDT FIGMGREVEN LIKENSELLD MKNALNIVKN DLINQVDELN SENMILRDEN 370 380 390 400 410 420 LSRQMVSEKM QEQITKHEEE IKTLKQKLME KENEQEEDDV PMAMRKRFTR SEMQRVLMDR 430 440 450 460 470 480 NAYKEKLMEL EESIKWTEMQ RAKKMQQQQQ NVNQKKSGGI WEFFSSLLGD SVTPPASSRG 490 500 510 520 530 540 NRASSSRGKM TRSVEYIDPD MISERRAAER REQYKLVREH VKKEDGRIEA YGWSLPNVEA 550 560 570 580 590 600 EVSSVPIPVC CRPLLDNEPS LKIWCATGVV LRGGRDERGQ WIVGDPIYFA PASMKKTKTS 610 620 630 640 650 660 NHRPELEDEI KRARNLDARE SELDEWQSSS LVWVVSSNQG KSLIAVLDAN NPNNIIETFP 670 680 690 700 710 720 ACDSHLLCIQ AVSGVMEGEP EMNEEQSKKY LSGGGKIKDL PEGLDGTDLG ACEWVELRKM 730 740 750 760 770 780 EDSEDGVPTY CSNDMKPSPK RTRDFSISEV APVDSSAPVK EDPLPPPANR PGGRAALPPH 790 800 810 820 830 840 IRDAMSKYDG VSGQMSGALP TVWMGGQNQY IYIHSAVTAW KQCLRRIKMP DAVLSIVHYK 850 860 870 880 890 900 SRIFAALANG TIAIFHRNKH GEWSDEGYHS LRVGSATSSV RSLCLVSTNI WATYKNCVVV 910 920 930 940 950 960 LDAESLQIVK VFAAHPRKDS QVRNMQWVGA GVWLSIRLDS TLRLYHAHTY EHLQDVDIEP 970 980 990 1000 1010 1020 YVTKMLGTSK LDFSYMRTTA LLVSNRRLWI GTGTGVIISV PFSGQLEKKI ETKDSKRPAG 1030 1040 1050 1060 1070 1080 PGGLVRVYGA TSENATNDEK TNDDFIPYCN LAHAQLSFHG HKDSVKFFLG VPGASKNGED 1090 1100 1110 1120 1130 ESAEVTLRRM LIMSGGDGYI DFRIGEENEP ELTGQSIRPR DMSHLIIWEV DAELPILSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Caenorhabditis elegans JNK signal transduction pathway regulates coordinated movement via type-D GABAergic motor neurons." Kawasaki M., Hisamoto N., Iino Y., Yamamoto M., Ninomiya-Tsuji J., Matsumoto K. EMBO J. 18:3604-3615(1999) [PubMed: 10393177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), FUNCTION, INTERACTION WITH JNK-1; JKK-1 AND SEK-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[4]	"UNC-16, a JNK-signaling scaffold protein, regulates vesicle transport in C. elegans." Byrd D.T., Kawasaki M., Walcoff M., Hisamoto N., Matsumoto K., Jin Y. Neuron 32:787-800(2001) [PubMed: 11738026] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH JNK-1; JKK-1 AND SEK-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF424978 mRNA. Translation: AAL23934.1. Z22176 Genomic DNA. Translation: CAA80140.1. Z22176 Genomic DNA. Translation: CAD45611.1.
PIR	S40932.
RefSeq	NP_001022981.1. NP_741263.3.
UniGene	Cel.34202
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25590N.
IntAct	P34609. 9 interactions.
STRING	P34609.
Proteomic databases
PRIDE	P34609.
Genome annotation databases
Ensembl	ZK1098.10b.1; ZK1098.10b.1; ZK1098.10; Caenorhabditis elegans. [Genome view] ZK1098.10b.2; ZK1098.10b.2; ZK1098.10; Caenorhabditis elegans. [Genome view]
GeneID	176349.
KEGG	cel:ZK1098.10.
UCSC	ZK1098.10b.1. c. elegans.
Organism-specific databases
CTD	176349.
WormBase	WBGene00006755. unc-16.
WormPep	ZK1098.10a. CE00363. [WorfDB] ZK1098.10b. CE31846. [WorfDB]
Phylogenomic databases
OMA	VKTLMPL
Gene expression databases
ArrayExpress	P34609.
Family and domain databases
InterPro	IPR019143. JNK/Rab-associated_protein-1. IPR011044. Quino_amine_DH_bsu. [Graphical view]
Pfam	PF09744. Jnk-SapK_ap_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	892188.

Entry information

Entry name

JIP_CAEEL

Accession

Primary (citable) accession number: P34609
Secondary accession number(s): Q95V72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 25, 2002
Last modified:	November 24, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project