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P34579 (UNC47_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular GABA transporter
Alternative name(s):
Uncoordinated protein 47
Short name=Protein unc-47
Gene names
Name:unc-47
ORF Names:T20G5.6
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the uptake of GABA into the synaptic vesicles. Ref.1

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein Ref.1.

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processneurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

synaptic vesicle

Inferred from direct assay Ref.1. Source: WormBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Vesicular GABA transporter
PRO_0000093824

Regions

Topological domain1 – 9393Cytoplasmic Potential
Transmembrane94 – 11421Helical; Potential
Topological domain115 – 1195Lumenal, vesicle Potential
Transmembrane120 – 14021Helical; Potential
Topological domain141 – 16727Cytoplasmic Potential
Transmembrane168 – 18821Helical; Potential
Topological domain189 – 20315Lumenal, vesicle Potential
Transmembrane204 – 22421Helical; Potential
Topological domain225 – 2284Cytoplasmic Potential
Transmembrane229 – 24921Helical; Potential
Topological domain250 – 26314Lumenal, vesicle Potential
Transmembrane264 – 28421Helical; Potential
Topological domain285 – 30521Cytoplasmic Potential
Transmembrane306 – 32621Helical; Potential
Topological domain327 – 34115Lumenal, vesicle Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 39836Cytoplasmic Potential
Transmembrane399 – 41921Helical; Potential
Topological domain420 – 4212Lumenal, vesicle Potential
Transmembrane422 – 44221Helical; Potential
Topological domain443 – 45715Cytoplasmic Potential
Transmembrane458 – 47821Helical; Potential
Topological domain479 – 4868Lumenal, vesicle Potential

Amino acid modifications

Glycosylation3371N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis4621G → R in N2409; loss of GABA transport into synaptic vesicles. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P34579 [UniParc].

Last modified July 11, 2001. Version 2.
Checksum: 6435C3B338552925

FASTA48654,439
        10         20         30         40         50         60 
MASNRFQNLQ NWTNKHVFSN SLDYWNQELN EVPSYQNQPQ TGESGSNPPP HDRLEPIQES 

        70         80         90        100        110        120 
VVSEQPQKDD INKQEEAKDD GHGEASEPIS ALQAAWNVTN AIQGMFIVGL PIAVKVGGWW 

       130        140        150        160        170        180 
SIGAMVGVAY VCYWTGVLLI ECLYENGVKK RKTYREIADF YKPGFGKWVL AAQLTELLST 

       190        200        210        220        230        240 
CIIYLVLAAD LLQSCFPSVD KAGWMMITSA SLLTCSFLDD LQIVSRLSFF NAISHLIVNL 

       250        260        270        280        290        300 
IMVLYCLSFV SQWSFSTITF SLNINTLPTI VGMVVFGYTS HIFLPNLEGN MKNPAQFNVM 

       310        320        330        340        350        360 
LKWSHIAAAV FKVVFGMLGF LTFGELTQEE ISNSLPNQSF KILVNLILVV KALLSYPLPF 

       370        380        390        400        410        420 
YAAVQLLKNN LFLGYPQTPF TSCYSPDKSL REWAVTLRII LVLFTLFVAL SVPYLVELMG 

       430        440        450        460        470        480 
LVGNITGTML SFIWPALFHL YIKEKTLNNF EKRFDQGIII MGCSVCISGV YFSSMELLRA 


INSADS

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of the vesicular GABA transporter."
McIntire S.L., Reimer R.J., Schuske K., Edwards R.H., Jorgensen E.M.
Nature 389:870-876(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-462.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF031935 mRNA. Translation: AAB87066.1.
Z30423 Genomic DNA. Translation: CAA83006.2.
PIRS42372.
T42254.
RefSeqNP_499255.1. NM_066854.5.
UniGeneCel.19679.

3D structure databases

ProteinModelPortalP34579.
ModBaseSearch...

Protein family/group databases

TCDB2.A.18.5.1. amino acid/auxin permease (AAAP) family.

Proteomic databases

PaxDbP34579.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT20G5.6.1; T20G5.6.1; T20G5.6.
T20G5.6.2; T20G5.6.2; T20G5.6.
GeneID176431.
KEGGcel:CELE_T20G5.6.
UCSCT20G5.6.2. c. elegans.

Organism-specific databases

CTD176431.
WormBaseT20G5.6; CE25119; WBGene00006783; unc-47.

Phylogenomic databases

eggNOGCOG0814.
GeneTreeENSGT00490000043380.
HOGENOMHOG000220837.
InParanoidP34579.
KOK15015.
OMAMAIFIPH.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio892552.

Entry information

Entry nameUNC47_CAEEL
AccessionPrimary (citable) accession number: P34579
Secondary accession number(s): O17475
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 11, 2001
Last modified: May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

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