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Protein

Probable citrate synthase, mitochondrial

Gene

cts-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathway:itricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable citrate synthase, mitochondrial (cts-1)
  2. Probable aconitate hydratase, mitochondrial (aco-2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei303 – 3031PROSITE-ProRule annotation
Active sitei349 – 3491PROSITE-ProRule annotation
Active sitei404 – 4041PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiREACT_287541. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable citrate synthase, mitochondrial (EC:2.3.3.16)
Gene namesi
Name:cts-1
ORF Names:T20G5.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT20G5.2; CE00513; WBGene00000833; cts-1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 468Probable citrate synthase, mitochondrialPRO_0000005470
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PaxDbiP34575.

2D gel databases

World-2DPAGE0020:P34575.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi6239.T20G5.2.2.

Structurei

3D structure databases

ProteinModelPortaliP34575.
SMRiP34575. Positions 32-462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
GeneTreeiENSGT00390000006813.
HOGENOMiHOG000130831.
InParanoidiP34575.
KOiK01647.
OMAiINRTKYW.
PhylomeDBiP34575.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSGMAIRR LITKGVIPVC QVAPLSTSAE GSTNLKEVLS KKIPAHNAKV
60 70 80 90 100
KSFRTEHGST VVQNVNIDMI YGGMRSMKGM VTETSVLDPE EGIRFRGYSI
110 120 130 140 150
PECQKLLPKA KGGEEPLPEA IWWLLCTGDV PSEAQTAAIT KEWNARADLP
160 170 180 190 200
THVVRMLDNF PDNLHPMAQF IAAIAALNNE SKFAGAYARG VAKASYWEYA
210 220 230 240 250
YEDSMDLLAK LPTVAAIIYR NLYRDGSAVS VIDPKKDWSA NFSSMLGYDD
260 270 280 290 300
PLFAELMRLY LVIHSDHEGG NVSAHTSHLV GSALSDPYLS FSAAMAGLAG
310 320 330 340 350
PLHGLANQEV LVFLNKIVGE IGFNYTEEQL KEWVWKHLKS GQVVPGYGHA
360 370 380 390 400
VLRKTDPRYE CQREFALKHL PNDDLFKLVS TLYKITPGIL LEQGKAKNPW
410 420 430 440 450
PNVDSHSGVL LQYFGMTEMS FYTVLFGVSR ALGCLSQLIW ARGMGLPLER
460
PKSHSTDGLI KLALAAKK
Length:468
Mass (Da):51,540
Last modified:February 1, 1994 - v1
Checksum:iCDE40CCA5729B9EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30423 Genomic DNA. Translation: CAA83004.1.
PIRiS42370.
RefSeqiNP_499264.1. NM_066863.6.
UniGeneiCel.17843.

Genome annotation databases

EnsemblMetazoaiT20G5.2; T20G5.2; WBGene00000833.
GeneIDi176437.
KEGGicel:CELE_T20G5.2.
UCSCiT20G5.2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30423 Genomic DNA. Translation: CAA83004.1.
PIRiS42370.
RefSeqiNP_499264.1. NM_066863.6.
UniGeneiCel.17843.

3D structure databases

ProteinModelPortaliP34575.
SMRiP34575. Positions 32-462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T20G5.2.2.

2D gel databases

World-2DPAGE0020:P34575.

Proteomic databases

PaxDbiP34575.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT20G5.2; T20G5.2; WBGene00000833.
GeneIDi176437.
KEGGicel:CELE_T20G5.2.
UCSCiT20G5.2.1. c. elegans.

Organism-specific databases

CTDi176437.
WormBaseiT20G5.2; CE00513; WBGene00000833; cts-1.

Phylogenomic databases

eggNOGiCOG0372.
GeneTreeiENSGT00390000006813.
HOGENOMiHOG000130831.
InParanoidiP34575.
KOiK01647.
OMAiINRTKYW.
PhylomeDBiP34575.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
ReactomeiREACT_287541. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi892576.
PROiP34575.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiCISY_CAEEL
AccessioniPrimary (citable) accession number: P34575
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 22, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.