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Protein

General transcription factor IIH subunit 2

Gene

T16H12.4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri286 – 303C4-typeAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-113418. Formation of the Early Elongation Complex.
R-CEL-5696395. Formation of Incision Complex in GG-NER.
R-CEL-5696400. Dual Incision in GG-NER.
R-CEL-674695. RNA Polymerase II Pre-transcription Events.
R-CEL-6781823. Formation of TC-NER Pre-Incision Complex.
R-CEL-6782135. Dual incision in TC-NER.
R-CEL-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-CEL-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-CEL-72086. mRNA Capping.
R-CEL-73776. RNA Polymerase II Promoter Escape.
R-CEL-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-CEL-75953. RNA Polymerase II Transcription Initiation.
R-CEL-75955. RNA Polymerase II Transcription Elongation.
R-CEL-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-CEL-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 2
Alternative name(s):
TFIIH basal transcription factor complex subunit 2
Gene namesi
ORF Names:T16H12.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT16H12.4; CE29053; WBGene00011814.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192501 – 376General transcription factor IIH subunit 2Add BLAST376

Proteomic databases

EPDiP34567.
PaxDbiP34567.
PeptideAtlasiP34567.

Expressioni

Gene expression databases

BgeeiWBGene00011814.

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.By similarity

Protein-protein interaction databases

BioGridi41615. 1 interactor.
IntActiP34567. 1 interactor.
STRINGi6239.T16H12.4.1.

Structurei

3D structure databases

ProteinModelPortaliP34567.
SMRiP34567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 206VWFAPROSITE-ProRule annotationAdd BLAST143

Sequence similaritiesi

Belongs to the GTF2H2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri286 – 303C4-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2807. Eukaryota.
COG5151. LUCA.
GeneTreeiENSGT00490000043395.
HOGENOMiHOG000159415.
InParanoidiP34567.
KOiK03142.
OMAiDCDIFIH.
OrthoDBiEOG091G08ZR.
PhylomeDBiP34567.

Family and domain databases

CDDicd01453. vWA_transcription_factor_IIH_t. 1 hit.
Gene3Di3.30.40.10. 1 hit.
3.40.50.410. 1 hit.
InterProiView protein in InterPro
IPR007198. Ssl1-like.
IPR004595. TFIIH_C1-like_dom.
IPR012170. TFIIH_SSL1/p44.
IPR002035. VWF_A.
IPR036465. vWFA_dom_sf.
IPR013087. Znf_C2H2_type.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
PANTHERiPTHR12695. PTHR12695. 1 hit.
PfamiView protein in Pfam
PF07975. C1_4. 1 hit.
PF04056. Ssl1. 1 hit.
PIRSFiPIRSF015919. TFIIH_SSL1. 1 hit.
SMARTiView protein in SMART
SM01047. C1_4. 1 hit.
SM00327. VWA. 1 hit.
SUPFAMiSSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00622. ssl1. 1 hit.
PROSITEiView protein in PROSITE
PS50234. VWFA. 1 hit.
PS01357. ZF_ZZ_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P34567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDDEQKGYT WEAGYAEGLN INDVLVEDEG GSIEKSIAKY VADSKRKARL
60 70 80 90 100
TKRPERIRLG IMRHVMIVID CSRFMTSKAM PPSRFVVVMK ALQTFLDRFF
110 120 130 140 150
EQNPIAQIGL ITCKDRKADR LTMMTGNIRV LKESLNTLTE AFCGGDFSLQ
160 170 180 190 200
NALQLACANL KGMPGHVSRE VVLVISALST IDPGNIYSTI ETMKRMNIRC
210 220 230 240 250
SAIGLSAEMF VCKEMAKATK GEYSVALDPD HLQLLFSKHT LPPSSAKSSE
260 270 280 290 300
CNAIHVGFPH HELITTRSFC VCHPDTKPIS SRGFICTQCG ARHCSIPAEC
310 320 330 340 350
PVCKLTLVAA PQLARAFRHL QPLSAFEQIE VTRGFCYACE TRLSGEGFRC
360 370
GSCQLVFCLD CDTLLHESLH VCPGCN
Length:376
Mass (Da):41,611
Last modified:January 27, 2003 - v3
Checksum:i03E8CF77EA00766F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z30662 Genomic DNA. Translation: CAA83139.3.
PIRiS42385.
RefSeqiNP_499239.1. NM_066838.3.
UniGeneiCel.10041.

Genome annotation databases

EnsemblMetazoaiT16H12.4.1; T16H12.4.1; WBGene00011814.
T16H12.4.2; T16H12.4.2; WBGene00011814.
GeneIDi176421.
KEGGicel:CELE_T16H12.4.
UCSCiT16H12.4.1. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiTF2H2_CAEEL
AccessioniPrimary (citable) accession number: P34567
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 27, 2003
Last modified: March 28, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome