Reviewed, UniProtKB/Swiss-Prot P34563 (IF5A1_CAEEL)

Names and origin

Protein names

Recommended name:
    Eukaryotic translation initiation factor 5A-1
      Short name=eIF-5A-1
Alternative name(s):
    Initiation factor five protein 1

Gene names

Name:	iff-1
ORF Names:	T05G5.10

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis By similarity. Required for mitotic germ cell proliferation, gametogenesis after entry into meiosis, and localization of the P granule component pgl-1 on P granules. Ref.3
Subcellular location	Cytoplasm By similarity. Cytoplasm By similarity.
Tissue specificity	Expressed specifically in the germline in the distal region of gonads where germ cells actively proliferate. Ref.3
Developmental stage	Present at late larval stages and adults. Ref.3
Post-translational modification	eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Sequence similarities	Belongs to the eIF-5A family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Elongation factor
PTM	Hypusine
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro reproduction Ref.3 Inferred from mutant phenotype. Source: WormBase translation Inferred from electronic annotation. Source: UniProtKB-KW translational frameshifting Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	O45679	1	EBI-327278,EBI-313935
	P90908	1	EBI-327278,EBI-327298
	Q19835	1	EBI-327278,EBI-320617
	Q9XXJ0	2	EBI-327278,EBI-322139
ssp-31	Q9XXL3	1	EBI-327278,EBI-327302

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Eukaryotic translation initiation factor 5A-1		PRO_0000142456
Amino acid modifications
	Modified residue	54	1	Hypusine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34563-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 7BD03FAE52D6387C	FASTA	161	17,867
	10 20 30 40 50 60 MSEDHHDEEQ FDSAESGAAA TFPKQCSALR KNEHVMIRGR PCKIVEMSTS KTGKHGHAKV 70 80 90 100 110 120 HMVAIDIFTT KKLEDICPST HNMDVPVVKR REYILMSIED GFCSLMDPES CELKDDLKMP 130 140 150 160 EGDLGNTIRE ALEKDEGSVL VQVVAACGEE AILGYKISTK E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"The Caenorhabditis elegans eukaryotic initiation factor 5A homologue, IFF-1, is required for germ cell proliferation, gametogenesis and localization of the P-granule component PGL-1." Hanazawa M., Kawasaki I., Kunitomo H., Gengyo-Ando K., Bennett K.L., Mitani S., Iino Y. Mech. Dev. 121:213-224(2004) [PubMed: 15003625] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z27079 Genomic DNA. Translation: CAA81597.2. Different initiation.
PIR	S41010.
RefSeq	NP_499152.2.
UniGene	Cel.10082
3D structure databases
SMR	P34563. Positions 17-158.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25111N.
IntAct	P34563. 9 interactions.
STRING	P34563.
Genome annotation databases
Ensembl	T05G5.10; T05G5.10; T05G5.10; Caenorhabditis elegans. [Genome view]
GeneID	176373.
KEGG	cel:T05G5.10.
UCSC	T05G5.10. c. elegans.
Organism-specific databases
CTD	176373.
WormBase	WBGene00002064. iff-1.
WormPep	T05G5.10. CE37787. [WorfDB]
Phylogenomic databases
eggNOG	meNOG12378.
HOGENOM	HBG526951.
InParanoid	P34563.
PhylomeDB	P34563.
Gene expression databases
ArrayExpress	P34563.
Family and domain databases
InterPro	IPR005824. KOW. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR019769. Trans_elong_IF5A_hypusine_site. IPR001884. Transl_elong_IF5A. IPR020189. Transl_elong_IF5A_C. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
PANTHER	PTHR11673. EIF5A_hypusine. 1 hit.
Pfam	PF01287. eIF-5a. 1 hit. PF00467. KOW. 1 hit. [Graphical view]
PIRSF	PIRSF003025. eIF5A. 1 hit.
TIGRFAMs	TIGR00037. eIF_5A. 1 hit.
PROSITE	PS00302. IF5A_HYPUSINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF5A1_CAEEL

Accession

Primary (citable) accession number: P34563

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project