Reviewed, UniProtKB/Swiss-Prot P34559 (ECHM_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase, mitochondrial
    EC=4.2.1.17

Gene names

Name:	ech-6
ORF Names:	T05G5.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Homohexamer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P34605	1	EBI-329772,EBI-312149
	Q9N366	1	EBI-329772,EBI-331341
mdf-2	Q9NGT3	1	EBI-329772,EBI-328699

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Probable enoyl-CoA hydratase, mitochondrial		PRO_0000109262
Regions
	Region	96 – 99	4	Substrate binding By similarity
Sites
	Binding site	139	1	Substrate; via amide nitrogen By similarity
	Site	162	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34559-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: B7DFE711455CDCF2	FASTA	288	31,172
	10 20 30 40 50 60 MMRFSSMLVR NAKLCANVNQ MQVAAFSSKA PEMIKIEKVG EKQNVALIKL NRPKALNALC 70 80 90 100 110 120 AQLMTELADA LEVLDTDKSV GAIVITGSER AFAAGADIKE MTNNEFATTF SGSFLSNWTA 130 140 150 160 170 180 VSDVKKPVIA AVNGFALGGG NELAMMCDII YAGEKARFGQ PEINIGTIPG AGGTQRWARA 190 200 210 220 230 240 AGKSFAMEVC LTGNHVTAQE AKEHGIVSKI FPADQVVGEA VKLGEKIADQ SPLIVQMAKE 250 260 270 280 AVNKAYELTL QEGLHFERRL FHATFATKDR KEGMTAFAEK RKPQWESK

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z27079 Genomic DNA. Translation: CAA81594.1.
PIR	S41006.
RefSeq	NP_499156.1.
UniGene	Cel.4890
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26850N.
IntAct	P34559. 5 interactions.
STRING	P34559.
Genome annotation databases
Ensembl	T05G5.6.1; T05G5.6.1; T05G5.6; Caenorhabditis elegans. [Genome view] T05G5.6.2; T05G5.6.2; T05G5.6; Caenorhabditis elegans. [Genome view]
GeneID	176376.
KEGG	cel:T05G5.6.
UCSC	T05G5.6.1. c. elegans.
Organism-specific databases
CTD	176376.
WormBase	WBGene00001155. ech-6.
WormPep	T05G5.6. CE00318. [WorfDB]
Phylogenomic databases
OMA	GVEYERR.
Enzyme and pathway databases
BRENDA	4.2.1.17. 672.
Gene expression databases
ArrayExpress	P34559.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	892310.

Entry information

Entry name

ECHM_CAEEL

Accession

Primary (citable) accession number: P34559

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project