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P34556

- CDK1_CAEEL

UniProt

P34556 - CDK1_CAEEL

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Protein

Cyclin-dependent kinase 1

Gene

cdk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation both activates and inactivates the enzyme depending on the site of phosphorylation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511ATPPROSITE-ProRule annotation
Active sitei146 – 1461Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 369ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: WormBase
  3. protein kinase binding Source: UniProtKB
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell division Source: UniProtKB
  2. meiotic nuclear division Source: WormBase
  3. mitotic nuclear division Source: WormBase
  4. oocyte maturation Source: WormBase
  5. positive regulation of meiosis Source: WormBase
  6. positive regulation of meiotic cell cycle Source: UniProtKB
  7. positive regulation of protein serine/threonine kinase activity Source: WormBase
  8. protein phosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1045.
ReactomeiREACT_183103. G1/S-Specific Transcription.
REACT_208370. Depolymerisation of the Nuclear Lamina.
REACT_209994. Regulation of APC/C activators between G1/S and early anaphase.
REACT_216568. Resolution of Sister Chromatid Cohesion.
REACT_218728. Cyclin A/B1 associated events during G2/M transition.
REACT_221191. G2/M DNA replication checkpoint.
REACT_234286. E2F-enabled inhibition of pre-replication complex formation.
REACT_237648. E2F mediated regulation of DNA replication.
REACT_250477. Cyclin B2 mediated events.
REACT_257283. Activation of NIMA Kinases NEK9, NEK6, NEK7.
REACT_259352. ERK1 activation.
SignaLinkiP34556.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:cdk-1
Synonyms:ncc-1
ORF Names:T05G5.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiT05G5.3; CE00315; WBGene00000405; cdk-1.

Subcellular locationi

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. cytoskeleton Source: UniProtKB-KW
  3. nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi173 – 1731I → F: No affect on cks-1 binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Cyclin-dependent kinase 1PRO_0000085738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphothreonineBy similarity
Modified residuei33 – 331PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP34556.
PRIDEiP34556.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cks-1.1 Publication

Protein-protein interaction databases

BioGridi41569. 3 interactions.
DIPiDIP-26477N.
IntActiP34556. 4 interactions.
MINTiMINT-1082928.
STRINGi6239.T05G5.3.3.

Structurei

3D structure databases

ProteinModelPortaliP34556.
SMRiP34556. Positions 18-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 312291Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119154.
InParanoidiP34556.
KOiK02087.
OMAiAKRACTH.
PhylomeDBiP34556.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34556-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPIREGEVA HEGDSVYTLN DFTKLEKIGE GTYGVVYKGK NRRTNAMVAM
60 70 80 90 100
KKIRLESEDE GVPSTAVREI SLLKELQHPN VVGLEAVIMQ ENRLFLIFEF
110 120 130 140 150
LSFDLKRYMD QLGKDEYLPL ETLKSYTFQI LQAMCFCHQR RVIHRDLKPQ
160 170 180 190 200
NLLVDNNGAI KLADFGLARA IGIPIRVYTH EVVTLWYRAP EILMGAQRYS
210 220 230 240 250
MGVDMWSIGC IFAEMATKKP LFQGDSEIDE LFRIFRVLGT PTELEWNGVE
260 270 280 290 300
SLPDYKATFP KWRENFLRDK FYDKKTGKHL LDDTAFSLLE GLLIYDPSLR
310 320 330
LNAKKALVHP YFDNMDTSKL PAGNYRGELE LF
Length:332
Mass (Da):38,295
Last modified:February 1, 1994 - v1
Checksum:iB6297E92949C8206
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761R → P in AAC60520. (PubMed:7830726)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68384 mRNA. Translation: CAA48455.1.
S75262 mRNA. Translation: AAC60520.1.
AF129109 mRNA. Translation: AAD37119.1.
Z27079 Genomic DNA. Translation: CAA81590.1.
PIRiS41003.
RefSeqiNP_001022747.1. NM_001027576.3.
UniGeneiCel.23379.

Genome annotation databases

EnsemblMetazoaiT05G5.3.1; T05G5.3.1; WBGene00000405.
T05G5.3.2; T05G5.3.2; WBGene00000405.
GeneIDi176374.
KEGGicel:CELE_T05G5.3.
UCSCiT05G5.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68384 mRNA. Translation: CAA48455.1 .
S75262 mRNA. Translation: AAC60520.1 .
AF129109 mRNA. Translation: AAD37119.1 .
Z27079 Genomic DNA. Translation: CAA81590.1 .
PIRi S41003.
RefSeqi NP_001022747.1. NM_001027576.3.
UniGenei Cel.23379.

3D structure databases

ProteinModelPortali P34556.
SMRi P34556. Positions 18-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41569. 3 interactions.
DIPi DIP-26477N.
IntActi P34556. 4 interactions.
MINTi MINT-1082928.
STRINGi 6239.T05G5.3.3.

Proteomic databases

PaxDbi P34556.
PRIDEi P34556.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T05G5.3.1 ; T05G5.3.1 ; WBGene00000405 .
T05G5.3.2 ; T05G5.3.2 ; WBGene00000405 .
GeneIDi 176374.
KEGGi cel:CELE_T05G5.3.
UCSCi T05G5.3.1. c. elegans.

Organism-specific databases

CTDi 176374.
WormBasei T05G5.3 ; CE00315 ; WBGene00000405 ; cdk-1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119154.
InParanoidi P34556.
KOi K02087.
OMAi AKRACTH.
PhylomeDBi P34556.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 1045.
Reactomei REACT_183103. G1/S-Specific Transcription.
REACT_208370. Depolymerisation of the Nuclear Lamina.
REACT_209994. Regulation of APC/C activators between G1/S and early anaphase.
REACT_216568. Resolution of Sister Chromatid Cohesion.
REACT_218728. Cyclin A/B1 associated events during G2/M transition.
REACT_221191. G2/M DNA replication checkpoint.
REACT_234286. E2F-enabled inhibition of pre-replication complex formation.
REACT_237648. E2F mediated regulation of DNA replication.
REACT_250477. Cyclin B2 mediated events.
REACT_257283. Activation of NIMA Kinases NEK9, NEK6, NEK7.
REACT_259352. ERK1 activation.
SignaLinki P34556.

Miscellaneous databases

NextBioi 892302.
PROi P34556.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of a cDNA coding for a p34-cdc2-like protein from Caenorhabiditis elegans."
    Ferraz C., Thierry-Mieg D., Le Peuch C.J.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "The identification of a Caenorhabditis elegans homolog of p34cdc2 kinase."
    Mori H., Palmer R.E., Sternberg P.W.
    Mol. Gen. Genet. 245:781-786(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The Caenorhabditis elegans gene ncc-1 encodes a cdc2-related kinase required for M phase in meiotic and mitotic cell divisions, but not for S phase."
    Boxem M., Srinivasan D.G., van den Heuvel S.
    Development 126:2227-2239(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  5. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  6. "The conserved kinases CDK-1, GSK-3, KIN-19, and MBK-2 promote OMA-1 destruction to regulate the oocyte-to-embryo transition in C. elegans."
    Shirayama M., Soto M.C., Ishidate T., Kim S., Nakamura K., Bei Y., van den Heuvel S., Mello C.C.
    Curr. Biol. 16:47-55(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CKS-1, ENZYME REGULATION, MUTAGENESIS OF ILE-173.

Entry informationi

Entry nameiCDK1_CAEEL
AccessioniPrimary (citable) accession number: P34556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3