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Protein

Cyclin-dependent kinase 1

Gene

cdk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation both activates and inactivates the enzyme depending on the site of phosphorylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51ATPPROSITE-ProRule annotation1
Active sitei146Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 36ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: WormBase
  • protein kinase binding Source: UniProtKB
  • RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  • anterior/posterior axis specification, embryo Source: WormBase
  • cell division Source: UniProtKB
  • meiotic nuclear division Source: WormBase
  • mitotic nuclear division Source: WormBase
  • oocyte maturation Source: WormBase
  • positive regulation of meiotic cell cycle Source: UniProtKB
  • positive regulation of meiotic nuclear division Source: WormBase
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: WormBase
  • positive regulation of protein serine/threonine kinase activity Source: WormBase
  • protein phosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1045.
ReactomeiR-CEL-110056. MAPK3 (ERK1) activation.
R-CEL-113507. E2F-enabled inhibition of pre-replication complex formation.
R-CEL-113510. E2F mediated regulation of DNA replication.
R-CEL-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-CEL-2299718. Condensation of Prophase Chromosomes.
R-CEL-4419969. Depolymerisation of the Nuclear Lamina.
R-CEL-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-CEL-69205. G1/S-Specific Transcription.
R-CEL-69273. Cyclin A/B1 associated events during G2/M transition.
R-CEL-69478. G2/M DNA replication checkpoint.
SignaLinkiP34556.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:cdk-1
Synonyms:ncc-1
ORF Names:T05G5.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiT05G5.3; CE00315; WBGene00000405; cdk-1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • microtubule organizing center Source: UniProtKB-SubCell
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173I → F: No affect on cks-1 binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857381 – 332Cyclin-dependent kinase 1Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphothreonineBy similarity1
Modified residuei33PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP34556.
PaxDbiP34556.
PeptideAtlasiP34556.
PRIDEiP34556.

PTM databases

iPTMnetiP34556.

Expressioni

Gene expression databases

BgeeiWBGene00000405.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cks-1.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi41569. 3 interactors.
DIPiDIP-26477N.
IntActiP34556. 4 interactors.
MINTiMINT-1082928.
STRINGi6239.T05G5.3.2.

Structurei

3D structure databases

ProteinModelPortaliP34556.
SMRiP34556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 312Protein kinasePROSITE-ProRule annotationAdd BLAST291

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
GeneTreeiENSGT00830000128256.
InParanoidiP34556.
KOiK02087.
OMAiGKMALKH.
OrthoDBiEOG091G0CH0.
PhylomeDBiP34556.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPIREGEVA HEGDSVYTLN DFTKLEKIGE GTYGVVYKGK NRRTNAMVAM
60 70 80 90 100
KKIRLESEDE GVPSTAVREI SLLKELQHPN VVGLEAVIMQ ENRLFLIFEF
110 120 130 140 150
LSFDLKRYMD QLGKDEYLPL ETLKSYTFQI LQAMCFCHQR RVIHRDLKPQ
160 170 180 190 200
NLLVDNNGAI KLADFGLARA IGIPIRVYTH EVVTLWYRAP EILMGAQRYS
210 220 230 240 250
MGVDMWSIGC IFAEMATKKP LFQGDSEIDE LFRIFRVLGT PTELEWNGVE
260 270 280 290 300
SLPDYKATFP KWRENFLRDK FYDKKTGKHL LDDTAFSLLE GLLIYDPSLR
310 320 330
LNAKKALVHP YFDNMDTSKL PAGNYRGELE LF
Length:332
Mass (Da):38,295
Last modified:February 1, 1994 - v1
Checksum:iB6297E92949C8206
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176R → P in AAC60520 (PubMed:7830726).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68384 mRNA. Translation: CAA48455.1.
S75262 mRNA. Translation: AAC60520.1.
AF129109 mRNA. Translation: AAD37119.1.
Z27079 Genomic DNA. Translation: CAA81590.1.
PIRiS41003.
RefSeqiNP_001022747.1. NM_001027576.3.
UniGeneiCel.23379.

Genome annotation databases

EnsemblMetazoaiT05G5.3.1; T05G5.3.1; WBGene00000405.
T05G5.3.2; T05G5.3.2; WBGene00000405.
GeneIDi176374.
KEGGicel:CELE_T05G5.3.
UCSCiT05G5.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68384 mRNA. Translation: CAA48455.1.
S75262 mRNA. Translation: AAC60520.1.
AF129109 mRNA. Translation: AAD37119.1.
Z27079 Genomic DNA. Translation: CAA81590.1.
PIRiS41003.
RefSeqiNP_001022747.1. NM_001027576.3.
UniGeneiCel.23379.

3D structure databases

ProteinModelPortaliP34556.
SMRiP34556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41569. 3 interactors.
DIPiDIP-26477N.
IntActiP34556. 4 interactors.
MINTiMINT-1082928.
STRINGi6239.T05G5.3.2.

PTM databases

iPTMnetiP34556.

Proteomic databases

EPDiP34556.
PaxDbiP34556.
PeptideAtlasiP34556.
PRIDEiP34556.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05G5.3.1; T05G5.3.1; WBGene00000405.
T05G5.3.2; T05G5.3.2; WBGene00000405.
GeneIDi176374.
KEGGicel:CELE_T05G5.3.
UCSCiT05G5.3.1. c. elegans.

Organism-specific databases

CTDi176374.
WormBaseiT05G5.3; CE00315; WBGene00000405; cdk-1.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
GeneTreeiENSGT00830000128256.
InParanoidiP34556.
KOiK02087.
OMAiGKMALKH.
OrthoDBiEOG091G0CH0.
PhylomeDBiP34556.

Enzyme and pathway databases

BRENDAi2.7.11.22. 1045.
ReactomeiR-CEL-110056. MAPK3 (ERK1) activation.
R-CEL-113507. E2F-enabled inhibition of pre-replication complex formation.
R-CEL-113510. E2F mediated regulation of DNA replication.
R-CEL-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-CEL-2299718. Condensation of Prophase Chromosomes.
R-CEL-4419969. Depolymerisation of the Nuclear Lamina.
R-CEL-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-CEL-69205. G1/S-Specific Transcription.
R-CEL-69273. Cyclin A/B1 associated events during G2/M transition.
R-CEL-69478. G2/M DNA replication checkpoint.
SignaLinkiP34556.

Miscellaneous databases

PROiP34556.

Gene expression databases

BgeeiWBGene00000405.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK1_CAEEL
AccessioniPrimary (citable) accession number: P34556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.