Cyclin-dependent kinase 1 - Caenorhabditis elegans

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P34556 (CDK1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 1
Short name=CDK1
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase

Gene names

Name:	cdk-1
Synonyms:	ncc-1
ORF Names:	T05G5.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation both activates and inactivates the enzyme depending on the site of phosphorylation. Ref.6
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cks-1. Ref.6
Subcellular location	Nucleus By similarity. Cytoplasm › cytoskeleton › centrosome By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Inferred from mutant phenotype Ref.3. Source: WormBase cell division Inferred from mutant phenotype Ref.3. Source: UniProtKB meiosis Inferred from mutant phenotype Ref.3. Source: WormBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW oocyte maturation Inferred from mutant phenotype PubMed 16421191. Source: WormBase positive regulation of meiosis Inferred from mutant phenotype PubMed 16421191. Source: WormBase positive regulation of meiotic cell cycle Inferred from mutant phenotype Ref.3. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.3. Source: WormBase microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.3. Source: WormBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay PubMed 19829076. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 332

332

Cyclin-dependent kinase 1

PRO_0000085738

Regions

Domain

22 – 312

291

Protein kinase

Nucleotide binding

28 – 36

ATP By similarity

Sites

Active site

146

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Experimental info

Mutagenesis

173

I → F: No affect on cks-1 binding. Ref.6

Sequence conflict

176

R → P in AAC60520. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P34556 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: B6297E92949C8206
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FASTA

332

38,295

        10         20         30         40         50         60 
MDPIREGEVA HEGDSVYTLN DFTKLEKIGE GTYGVVYKGK NRRTNAMVAM KKIRLESEDE 

        70         80         90        100        110        120 
GVPSTAVREI SLLKELQHPN VVGLEAVIMQ ENRLFLIFEF LSFDLKRYMD QLGKDEYLPL 

       130        140        150        160        170        180 
ETLKSYTFQI LQAMCFCHQR RVIHRDLKPQ NLLVDNNGAI KLADFGLARA IGIPIRVYTH 

       190        200        210        220        230        240 
EVVTLWYRAP EILMGAQRYS MGVDMWSIGC IFAEMATKKP LFQGDSEIDE LFRIFRVLGT 

       250        260        270        280        290        300 
PTELEWNGVE SLPDYKATFP KWRENFLRDK FYDKKTGKHL LDDTAFSLLE GLLIYDPSLR 

       310        320        330 
LNAKKALVHP YFDNMDTSKL PAGNYRGELE LF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of a cDNA coding for a p34-cdc2-like protein from Caenorhabiditis elegans." Ferraz C., Thierry-Mieg D., Le Peuch C.J. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"The identification of a Caenorhabditis elegans homolog of p34cdc2 kinase." Mori H., Palmer R.E., Sternberg P.W. Mol. Gen. Genet. 245:781-786(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The Caenorhabditis elegans gene ncc-1 encodes a cdc2-related kinase required for M phase in meiotic and mitotic cell divisions, but not for S phase." Boxem M., Srinivasan D.G., van den Heuvel S. Development 126:2227-2239(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[4]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[5]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[6]	"The conserved kinases CDK-1, GSK-3, KIN-19, and MBK-2 promote OMA-1 destruction to regulate the oocyte-to-embryo transition in C. elegans." Shirayama M., Soto M.C., Ishidate T., Kim S., Nakamura K., Bei Y., van den Heuvel S., Mello C.C. Curr. Biol. 16:47-55(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CKS-1, ENZYME REGULATION, MUTAGENESIS OF ILE-173.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68384 mRNA. Translation: CAA48455.1. S75262 mRNA. Translation: AAC60520.1. AF129109 mRNA. Translation: AAD37119.1. Z27079 Genomic DNA. Translation: CAA81590.1.
PIR	S41003.
RefSeq	NP_001022747.1. NM_001027576.3.
UniGene	Cel.23379.
3D structure databases
ProteinModelPortal	P34556.
SMR	P34556. Positions 18-315.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26477N.
IntAct	P34556. 4 interactions.
MINT	MINT-1082928.
STRING	6239.T05G5.3.3.
Proteomic databases
PaxDb	P34556.
PRIDE	P34556.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T05G5.3.1; T05G5.3.1; T05G5.3. T05G5.3.2; T05G5.3.2; T05G5.3.
GeneID	176374.
KEGG	cel:CELE_T05G5.3.
UCSC	T05G5.3.1. c. elegans.
Organism-specific databases
CTD	176374.
WormBase	T05G5.3; CE00315; WBGene00000405; cdk-1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00690000101791.
InParanoid	P34556.
KO	K02087.
OMA	IVAEMIL.
Enzyme and pathway databases
BRENDA	2.7.11.22. 1045.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	892302.

Entry information

Entry name

CDK1_CAEEL

Accession

Primary (citable) accession number: P34556

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents