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P34556

- CDK1_CAEEL

UniProt

P34556 - CDK1_CAEEL

Protein

Cyclin-dependent kinase 1

Gene

cdk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation both activates and inactivates the enzyme depending on the site of phosphorylation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511ATPPROSITE-ProRule annotation
    Active sitei146 – 1461Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 369ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: WormBase
    3. protein kinase binding Source: UniProtKB
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell division Source: UniProtKB
    2. meiotic nuclear division Source: WormBase
    3. mitotic nuclear division Source: WormBase
    4. oocyte maturation Source: WormBase
    5. positive regulation of meiosis Source: WormBase
    6. positive regulation of meiotic cell cycle Source: UniProtKB
    7. positive regulation of protein serine/threonine kinase activity Source: WormBase
    8. protein phosphorylation Source: WormBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 1045.
    ReactomeiREACT_183103. G1/S-Specific Transcription.
    REACT_209994. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_216568. Resolution of Sister Chromatid Cohesion.
    REACT_218728. Cyclin A/B1 associated events during G2/M transition.
    REACT_221191. G2/M DNA replication checkpoint.
    SignaLinkiP34556.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2 homolog
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:cdk-1
    Synonyms:ncc-1
    ORF Names:T05G5.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiT05G5.3; CE00315; WBGene00000405; cdk-1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: WormBase
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. nucleus Source: WormBase

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi173 – 1731I → F: No affect on cks-1 binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Cyclin-dependent kinase 1PRO_0000085738Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei32 – 321PhosphothreonineBy similarity
    Modified residuei33 – 331PhosphotyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP34556.
    PRIDEiP34556.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cks-1.1 Publication

    Protein-protein interaction databases

    BioGridi41569. 3 interactions.
    DIPiDIP-26477N.
    IntActiP34556. 4 interactions.
    MINTiMINT-1082928.
    STRINGi6239.T05G5.3.3.

    Structurei

    3D structure databases

    ProteinModelPortaliP34556.
    SMRiP34556. Positions 18-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 312291Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108415.
    InParanoidiP34556.
    KOiK02087.
    OMAiAKRACTH.
    PhylomeDBiP34556.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P34556-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPIREGEVA HEGDSVYTLN DFTKLEKIGE GTYGVVYKGK NRRTNAMVAM    50
    KKIRLESEDE GVPSTAVREI SLLKELQHPN VVGLEAVIMQ ENRLFLIFEF 100
    LSFDLKRYMD QLGKDEYLPL ETLKSYTFQI LQAMCFCHQR RVIHRDLKPQ 150
    NLLVDNNGAI KLADFGLARA IGIPIRVYTH EVVTLWYRAP EILMGAQRYS 200
    MGVDMWSIGC IFAEMATKKP LFQGDSEIDE LFRIFRVLGT PTELEWNGVE 250
    SLPDYKATFP KWRENFLRDK FYDKKTGKHL LDDTAFSLLE GLLIYDPSLR 300
    LNAKKALVHP YFDNMDTSKL PAGNYRGELE LF 332
    Length:332
    Mass (Da):38,295
    Last modified:February 1, 1994 - v1
    Checksum:iB6297E92949C8206
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti176 – 1761R → P in AAC60520. (PubMed:7830726)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68384 mRNA. Translation: CAA48455.1.
    S75262 mRNA. Translation: AAC60520.1.
    AF129109 mRNA. Translation: AAD37119.1.
    Z27079 Genomic DNA. Translation: CAA81590.1.
    PIRiS41003.
    RefSeqiNP_001022747.1. NM_001027576.3.
    UniGeneiCel.23379.

    Genome annotation databases

    EnsemblMetazoaiT05G5.3.1; T05G5.3.1; WBGene00000405.
    T05G5.3.2; T05G5.3.2; WBGene00000405.
    GeneIDi176374.
    KEGGicel:CELE_T05G5.3.
    UCSCiT05G5.3.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68384 mRNA. Translation: CAA48455.1 .
    S75262 mRNA. Translation: AAC60520.1 .
    AF129109 mRNA. Translation: AAD37119.1 .
    Z27079 Genomic DNA. Translation: CAA81590.1 .
    PIRi S41003.
    RefSeqi NP_001022747.1. NM_001027576.3.
    UniGenei Cel.23379.

    3D structure databases

    ProteinModelPortali P34556.
    SMRi P34556. Positions 18-315.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41569. 3 interactions.
    DIPi DIP-26477N.
    IntActi P34556. 4 interactions.
    MINTi MINT-1082928.
    STRINGi 6239.T05G5.3.3.

    Proteomic databases

    PaxDbi P34556.
    PRIDEi P34556.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T05G5.3.1 ; T05G5.3.1 ; WBGene00000405 .
    T05G5.3.2 ; T05G5.3.2 ; WBGene00000405 .
    GeneIDi 176374.
    KEGGi cel:CELE_T05G5.3.
    UCSCi T05G5.3.1. c. elegans.

    Organism-specific databases

    CTDi 176374.
    WormBasei T05G5.3 ; CE00315 ; WBGene00000405 ; cdk-1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108415.
    InParanoidi P34556.
    KOi K02087.
    OMAi AKRACTH.
    PhylomeDBi P34556.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 1045.
    Reactomei REACT_183103. G1/S-Specific Transcription.
    REACT_209994. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_216568. Resolution of Sister Chromatid Cohesion.
    REACT_218728. Cyclin A/B1 associated events during G2/M transition.
    REACT_221191. G2/M DNA replication checkpoint.
    SignaLinki P34556.

    Miscellaneous databases

    NextBioi 892302.
    PROi P34556.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of a cDNA coding for a p34-cdc2-like protein from Caenorhabiditis elegans."
      Ferraz C., Thierry-Mieg D., Le Peuch C.J.
      Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Bristol N2.
    2. "The identification of a Caenorhabditis elegans homolog of p34cdc2 kinase."
      Mori H., Palmer R.E., Sternberg P.W.
      Mol. Gen. Genet. 245:781-786(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The Caenorhabditis elegans gene ncc-1 encodes a cdc2-related kinase required for M phase in meiotic and mitotic cell divisions, but not for S phase."
      Boxem M., Srinivasan D.G., van den Heuvel S.
      Development 126:2227-2239(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Bristol N2.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    5. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    6. "The conserved kinases CDK-1, GSK-3, KIN-19, and MBK-2 promote OMA-1 destruction to regulate the oocyte-to-embryo transition in C. elegans."
      Shirayama M., Soto M.C., Ishidate T., Kim S., Nakamura K., Bei Y., van den Heuvel S., Mello C.C.
      Curr. Biol. 16:47-55(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CKS-1, ENZYME REGULATION, MUTAGENESIS OF ILE-173.

    Entry informationi

    Entry nameiCDK1_CAEEL
    AccessioniPrimary (citable) accession number: P34556
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3