Reviewed, UniProtKB/Swiss-Prot P34552 (ALX1_CAEEL)

Names and origin

Protein names

Recommended name:
    Apoptosis-linked gene 2-interacting protein X 1
Alternative name(s):
    Prion-like-(Q/N-rich) domain-bearing protein 58
    Protein YNK1

Gene names

Name:	alx-1
Synonyms:	pqn-58
ORF Names:	R10E12.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for lin-12 degradation after it has been internalised in the vulval precursor cells. Ref.4
Developmental stage	Expressed throughout development. Ref.1
Sequence similarities	Contains 1 BRO1 domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform b (identifier: P34552-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform a (identifier: P34552-2)

The sequence of this isoform differs from the canonical sequence as follows:
     722-742: Missing.

Isoform c (identifier: P34552-3)

The sequence of this isoform differs from the canonical sequence as follows:
     782-817: Missing.

Note: No experimental confirmation available.

Isoform d (identifier: P34552-4)

The sequence of this isoform differs from the canonical sequence as follows:
     359-742: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Apoptosis-linked gene 2-interacting protein X 1		PRO_0000058559
Regions
	Domain	5 – 398	394	BRO1
	Compositional bias	756 – 861	106	Gln-rich
Natural variations
	Alternative sequence	359 – 742	384	Missing in isoform d.		VSP_016611
	Alternative sequence	722 – 742	21	Missing in isoform a.		VSP_016612
	Alternative sequence	782 – 817	36	Missing in isoform c.		VSP_016613

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified December 20, 2005. Version 3. Checksum: 8596D6D69A8786D5	FASTA	882	98,264
	10 20 30 40 50 60 MATFGFLSAP LKSTNEVDLV KPLTSYIDNV YNTSDNNRSD VAEAVQELNK LRSKACCQPL 70 80 90 100 110 120 DKHQSALDVL TRYYDQLVAI ENKIIISATQ NPVVFKWKDA FDKGSLFSSR ASLSLSDGSF 130 140 150 160 170 180 ERAAVLFNIG SLMSQIGAAQ QFHTDDEIKV SAKLFQQSAG VFARLRDVVL GMVQQEPTPD 190 200 210 220 230 240 LMPDTLAALS ALMTAQAQEA IYIKGHKDKM KATSMVKISA QVAEFYSEAQ KMMSKDIVRG 250 260 270 280 290 300 LWDKDWSAIV SGKNLAYQAL AQYHQSEVCG EARQIGEQLS RLAESLKLFD TAQKYLPRDI 310 320 330 340 350 360 TGIWDIYPSV SKAHAAAKKD NDFIYHEKVS DFRTLPTLPK AVLAKPTPMQ TPMTPSFRDM 370 380 390 400 410 420 FAVLVPVQVH NAMQSYDARK AELVNMETVR MREATQLMNG VLASLNLPAA LDDVTSTETL 430 440 450 460 470 480 PESLKLKSAK LKQNGGSSEI MRLFSELPTL YQRNEDILTE TSRILNEEKE SDDTMRKQLG 490 500 510 520 530 540 TKWTRMSSEQ LTGPLVTEIG KYRGILHTAS NADKMVKEKF ESHRQGIELL SKNESELRSS 550 560 570 580 590 600 IPGQTAHATG ETDTVRQLRQ FMSQWNEVTT DRELLEKELK NTNCDIANDF LKAMAENQLI 610 620 630 640 650 660 NEEHISKEKI AQIFGDLKRR VQSSLDTQET LMNQIQAANN TFTGEKTGSS TGAERERILK 670 680 690 700 710 720 MLAQASDAYV ELKANLEEGT KFYNDLTPIL VRLQQKVSDF AFARQTEKED LMRQLQLSIV 730 740 750 760 770 780 SGQAAKAVVD GVNSLVSSYL TGGTNAAQSP ANAPPRPPPP RPAAPSVESP IPPPRTQQSM 790 800 810 820 830 840 QATPGAPPQY NPYQQQQQPQ MQQFQQHPGY YQQPMPYGQP QPMFQPQYQP TFAAPYPTFP 850 860 870 880 GAFPSYQQQW PQQQQQGGFP PNPQFGQQNQ QQGGGGGANP FQ
	Isoform a. Checksum: F2F600623FF74965	FASTA	861	96,246
	10 20 30 40 50 60 MATFGFLSAP LKSTNEVDLV KPLTSYIDNV YNTSDNNRSD VAEAVQELNK LRSKACCQPL 70 80 90 100 110 120 DKHQSALDVL TRYYDQLVAI ENKIIISATQ NPVVFKWKDA FDKGSLFSSR ASLSLSDGSF 130 140 150 160 170 180 ERAAVLFNIG SLMSQIGAAQ QFHTDDEIKV SAKLFQQSAG VFARLRDVVL GMVQQEPTPD 190 200 210 220 230 240 LMPDTLAALS ALMTAQAQEA IYIKGHKDKM KATSMVKISA QVAEFYSEAQ KMMSKDIVRG 250 260 270 280 290 300 LWDKDWSAIV SGKNLAYQAL AQYHQSEVCG EARQIGEQLS RLAESLKLFD TAQKYLPRDI 310 320 330 340 350 360 TGIWDIYPSV SKAHAAAKKD NDFIYHEKVS DFRTLPTLPK AVLAKPTPMQ TPMTPSFRDM 370 380 390 400 410 420 FAVLVPVQVH NAMQSYDARK AELVNMETVR MREATQLMNG VLASLNLPAA LDDVTSTETL 430 440 450 460 470 480 PESLKLKSAK LKQNGGSSEI MRLFSELPTL YQRNEDILTE TSRILNEEKE SDDTMRKQLG 490 500 510 520 530 540 TKWTRMSSEQ LTGPLVTEIG KYRGILHTAS NADKMVKEKF ESHRQGIELL SKNESELRSS 550 560 570 580 590 600 IPGQTAHATG ETDTVRQLRQ FMSQWNEVTT DRELLEKELK NTNCDIANDF LKAMAENQLI 610 620 630 640 650 660 NEEHISKEKI AQIFGDLKRR VQSSLDTQET LMNQIQAANN TFTGEKTGSS TGAERERILK 670 680 690 700 710 720 MLAQASDAYV ELKANLEEGT KFYNDLTPIL VRLQQKVSDF AFARQTEKED LMRQLQLSIV 730 740 750 760 770 780 SGTNAAQSPA NAPPRPPPPR PAAPSVESPI PPPRTQQSMQ ATPGAPPQYN PYQQQQQPQM 790 800 810 820 830 840 QQFQQHPGYY QQPMPYGQPQ PMFQPQYQPT FAAPYPTFPG AFPSYQQQWP QQQQQGGFPP 850 860 NPQFGQQNQQ QGGGGGANPF Q
	Isoform c. Checksum: B8124DFFD49D1A46	FASTA	846	93,987
	10 20 30 40 50 60 MATFGFLSAP LKSTNEVDLV KPLTSYIDNV YNTSDNNRSD VAEAVQELNK LRSKACCQPL 70 80 90 100 110 120 DKHQSALDVL TRYYDQLVAI ENKIIISATQ NPVVFKWKDA FDKGSLFSSR ASLSLSDGSF 130 140 150 160 170 180 ERAAVLFNIG SLMSQIGAAQ QFHTDDEIKV SAKLFQQSAG VFARLRDVVL GMVQQEPTPD 190 200 210 220 230 240 LMPDTLAALS ALMTAQAQEA IYIKGHKDKM KATSMVKISA QVAEFYSEAQ KMMSKDIVRG 250 260 270 280 290 300 LWDKDWSAIV SGKNLAYQAL AQYHQSEVCG EARQIGEQLS RLAESLKLFD TAQKYLPRDI 310 320 330 340 350 360 TGIWDIYPSV SKAHAAAKKD NDFIYHEKVS DFRTLPTLPK AVLAKPTPMQ TPMTPSFRDM 370 380 390 400 410 420 FAVLVPVQVH NAMQSYDARK AELVNMETVR MREATQLMNG VLASLNLPAA LDDVTSTETL 430 440 450 460 470 480 PESLKLKSAK LKQNGGSSEI MRLFSELPTL YQRNEDILTE TSRILNEEKE SDDTMRKQLG 490 500 510 520 530 540 TKWTRMSSEQ LTGPLVTEIG KYRGILHTAS NADKMVKEKF ESHRQGIELL SKNESELRSS 550 560 570 580 590 600 IPGQTAHATG ETDTVRQLRQ FMSQWNEVTT DRELLEKELK NTNCDIANDF LKAMAENQLI 610 620 630 640 650 660 NEEHISKEKI AQIFGDLKRR VQSSLDTQET LMNQIQAANN TFTGEKTGSS TGAERERILK 670 680 690 700 710 720 MLAQASDAYV ELKANLEEGT KFYNDLTPIL VRLQQKVSDF AFARQTEKED LMRQLQLSIV 730 740 750 760 770 780 SGQAAKAVVD GVNSLVSSYL TGGTNAAQSP ANAPPRPPPP RPAAPSVESP IPPPRTQQSM 790 800 810 820 830 840 QGQPQPMFQP QYQPTFAAPY PTFPGAFPSY QQQWPQQQQQ GGFPPNPQFG QQNQQQGGGG GANPFQ
	Isoform d. Checksum: 7B098D5B5F599A2D	FASTA	498	55,166
	10 20 30 40 50 60 MATFGFLSAP LKSTNEVDLV KPLTSYIDNV YNTSDNNRSD VAEAVQELNK LRSKACCQPL 70 80 90 100 110 120 DKHQSALDVL TRYYDQLVAI ENKIIISATQ NPVVFKWKDA FDKGSLFSSR ASLSLSDGSF 130 140 150 160 170 180 ERAAVLFNIG SLMSQIGAAQ QFHTDDEIKV SAKLFQQSAG VFARLRDVVL GMVQQEPTPD 190 200 210 220 230 240 LMPDTLAALS ALMTAQAQEA IYIKGHKDKM KATSMVKISA QVAEFYSEAQ KMMSKDIVRG 250 260 270 280 290 300 LWDKDWSAIV SGKNLAYQAL AQYHQSEVCG EARQIGEQLS RLAESLKLFD TAQKYLPRDI 310 320 330 340 350 360 TGIWDIYPSV SKAHAAAKKD NDFIYHEKVS DFRTLPTLPK AVLAKPTPMQ TPMTPSFRGT 370 380 390 400 410 420 NAAQSPANAP PRPPPPRPAA PSVESPIPPP RTQQSMQATP GAPPQYNPYQ QQQQPQMQQF 430 440 450 460 470 480 QQHPGYYQQP MPYGQPQPMF QPQYQPTFAA PYPTFPGAFP SYQQQWPQQQ QQGGFPPNPQ 490 FGQQNQQQGG GGGANPFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a splice variant of Caenorhabditis elegans YNK1, a putative element in signal transduction." Che S., Weil M.M., Etkin L.D., Epstein H.F., Kuang J. Biochim. Biophys. Acta 1354:231-240(1997) [PubMed: 9427532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[4]	"LIN-12/Notch trafficking and regulation of DSL ligand activity during vulval induction in Caenorhabditis elegans." Shaye D.D., Greenwald I. Development 132:5081-5092(2005) [PubMed: 16236769] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73679 mRNA. Translation: AAC67305.1. Z29561 Genomic DNA. Translation: CAA82667.2. Z29561 Genomic DNA. Translation: CAD54152.1. Z29561 Genomic DNA. Translation: CAD54153.1. Z29561 Genomic DNA. Translation: CAD91697.1.
PIR	S41034.
RefSeq	NP_001022713.1. NP_001022714.1. NP_001022715.1. NP_499213.1.
UniGene	Cel.19623
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P34552. 3 interactions.
STRING	P34552.
Proteomic databases
PRIDE	P34552.
Genome annotation databases
Ensembl	R10E12.1b; R10E12.1b; R10E12.1; Caenorhabditis elegans. [Genome view]
GeneID	176410.
KEGG	cel:R10E12.1.
NMPDR	fig|6239.3.peg.11326.
UCSC	R10E12.1a. c. elegans.
Organism-specific databases
CTD	176410.
WormBase	WBGene00007010. alx-1.
WormPep	R10E12.1a. CE25075. [WorfDB] R10E12.1b. CE00310. [WorfDB] R10E12.1c. CE31853. [WorfDB] R10E12.1d. CE34084. [WorfDB]
Phylogenomic databases
HOGENOM	HBG356869.
InParanoid	P34552.
OMA	RTMPPTK.
Gene expression databases
ArrayExpress	P34552.
Family and domain databases
InterPro	IPR004328. BRO1. [Graphical view]
Pfam	PF03097. BRO1. 1 hit. [Graphical view]
PROSITE	PS51180. BRO1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	892456.

Entry information

Entry name

ALX1_CAEEL

Accession

Primary (citable) accession number: P34552
Secondary accession number(s): Q7YXC7 , Q8I114, Q8I115, Q94159

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 20, 2005
Last modified:	December 15, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project