P34548 (SQV3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactosyltransferase sqv-3
EC=2.4.1.-

Alternative name(s):
Squashed vulva protein 3

Gene names

Name:	sqv-3
ORF Names:	R10E11.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chondroitin sulfate biosynthetic process Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase heparan sulfate proteoglycan biosynthetic process Inferred from mutant phenotype. Source: WormBase morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase oviposition Inferred from mutant phenotype. Source: WormBase regulation of cell morphogenesis Inferred from mutant phenotype. Source: WormBase vulval development Inferred from mutant phenotype. Source: WormBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Inferred from direct assay. Source: WormBase
Molecular function	galactosyltransferase activity Inferred from direct assay. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Probable galactosyltransferase sqv-3		PRO_0000080552
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 289	262	Lumenal Potential
Amino acid modifications
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	183	1	G → E in N2841. Ref.1
	Mutagenesis	275	1	V → F in N2823. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P34548 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 16C2C1F5AF35DC91	FASTA	289	33,807
	10 20 30 40 50 60 MKLKTRLILS GTILISLAAC YFLVLLVLDL EITRDLMTDY VDPRPLQTSY HKLCVIVPYR 70 80 90 100 110 120 DRLEELREFS PHMSKFLHNQ NVSHHILIIN QTDPLRFNRA SLINVGWNEA DRLGCDYMVM 130 140 150 160 170 180 NDVDLLPVNP EVPYDFPGIG VIRHITSPQY HPKYHYEKFI GGILMLTLKD YKKLNGMSNK 190 200 210 220 230 240 YWGWGLEDDE FYLRIIDSKL NLTRVSGLST DSSNTFRHIH GPKRKRDYTP KKNDKNQWEI 250 260 270 280 KRKRDHVSGL HDVRYLIDSR QLLDFSGTSV TIINVALHCD LNWTPYCKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway." Herman T., Horvitz H.R. Proc. Natl. Acad. Sci. U.S.A. 96:974-979(1999) [PubMed: 9927678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLY-183 AND PHE-275. Strain: Bristol N2.
[2]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005867 mRNA. Translation: CAA06744.1. Z29095 Genomic DNA. Translation: CAA82350.1.
PIR	S40716.
RefSeq	NP_499164.1. NM_066763.5.
UniGene	Cel.37560.
3D structure databases
ProteinModelPortal	P34548.
SMR	P34548. Positions 4-288.
ModBase	Search...
Protein-protein interaction databases
STRING	P34548.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	P34548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R10E11.4; R10E11.4; R10E11.4.
GeneID	176382.
KEGG	cel:R10E11.4.
NMPDR	fig|6239.3.peg.11262.
UCSC	R10E11.4. c. elegans.
Organism-specific databases
CTD	176382.
WormBase	R10E11.4; CE00306; WBGene00005021; sqv-3.
Phylogenomic databases
eggNOG	meNOG06459.
GeneTree	EMGT00050000004582.
HOGENOM	HBG315803.
InParanoid	P34548.
OMA	GWGLEDD.
PhylomeDB	P34548.
Gene expression databases
ArrayExpress	P34548.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2_met. [Graphical view]
KO	K00733.
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
Pfam	PF02709. Galactosyl_T_2. 1 hit. [Graphical view]
PRINTS	PR02050. B14GALTRFASE.
ProtoNet	Search...
Other
NextBio	892340.

Entry information

Entry name

SQV3_CAEEL

Accession

Primary (citable) accession number: P34548

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project