Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P34547

- UBP46_CAEEL

UniProt

P34547 - UBP46_CAEEL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 46

Gene

usp-46

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates the abundance of the glr-1 glutamate receptor in the ventral nerve cord by promoting its deubiquitination and preventing its degradation in the lysosome. Contributes to the regulation of embryonic polarity.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361NucleophilePROSITE-ProRule annotation
Active sitei342 – 3421Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ionotropic glutamate receptor binding Source: WormBase
  2. ubiquitin-specific protease activity Source: WormBase

GO - Biological processi

  1. protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: WormBase
  2. regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.A37.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 46 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme
Ubiquitin thioesterase
Ubiquitin-specific-processing protease
Gene namesi
Name:usp-46
ORF Names:R10E11.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiR10E11.3a; CE41150; WBGene00011216; usp-46.
R10E11.3b; CE41151; WBGene00011216; usp-46.

Subcellular locationi

Perikaryon 1 Publication. Cytoplasm 1 Publication
Note: In ventral nerve cord interneurons, localized to discrete puncta within the perikaryon. In the ventral nerve cord, localized diffusely throughout the cytoplasm and to punctate structures.

GO - Cellular componenti

  1. cell body Source: WormBase
  2. cytoplasm Source: WormBase
  3. endosome Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Decreased levels of glr-1 in the ventral nerve cord and corresponding defects in glr-1-dependent behaviors including reduced spontaneous reversal frequency and reduced responsiveness to the nose touch assay which normally induces backward locomotion. Increased levels of ubiquitinated glr-1.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 361C → A: Abolishes the increased glr-1 expression associated with coexpression of usp-46 with wdr-20 and wdr-48. Does not rescue the decreased glr-1 levels seen in usp-46 mutants. No effect on interaction with glr-1. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 426425Ubiquitin carboxyl-terminal hydrolase 46PRO_0000080684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP34547.
PRIDEiP34547.

Expressioni

Tissue specificityi

Expressed in a number of tissues including the nervous system, pharynx, body wall muscle, vulva muscle and intestine and is detected in many head and ventral cord neurons.1 Publication

Interactioni

Subunit structurei

Interacts with wdr-20 and wdr-48; the catalytic activity of usp-46 is increased in the presence of both wdr-20 and wdr-48. Interacts with glr-1; the interaction results in deubiquitination of glr-1.2 Publications

Protein-protein interaction databases

BioGridi41576. 2 interactions.
STRINGi6239.R10E11.3a.1.

Structurei

3D structure databases

ProteinModelPortaliP34547.
SMRiP34547. Positions 26-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 406380USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000231498.
InParanoidiP34547.
KOiK11842.
OMAiDANIQMP.
PhylomeDBiP34547.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: P34547-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGATGSSQLE KEISTTESVN NANEHYYGLV NFGNTCYCNS VIQALFFCRP
60 70 80 90 100
FREKVLNYKQ TLKKSGASKD NLVTCLADLF HSIASQKRRV GTIAPKRFIT
110 120 130 140 150
KLKKENELFD NYMQQDAHEF FNYLINTISE TLIQEKIAER EKASRHGTLK
160 170 180 190 200
KGNVTVNLAP ATAGLPRSDE KGTSERNGGI TVEGNEFLNK SDTTTWIHEI
210 220 230 240 250
FQGILTNETR CLSCETVSSK DEDFLDLSID VEQNTSISHC LRVFSETETL
260 270 280 290 300
CGDQKYFCET CSSKQEAQKR MRIKKPPQLL ALHLKRFKFV EPLNRHTKLS
310 320 330 340 350
YRVVFPLELR LFNVSDDAEY GDRMYDLVAT VVHCGATPNR GHYITLVKSN
360 370 380 390 400
SFWLVFDDDI VEKLEVSSME EFSGMSTDAN IQMPPGNQSA PQKNSESAYI
410 420
LFYQARDYAA DDPNHNHKGK NSTHSV
Length:426
Mass (Da):48,277
Last modified:December 4, 2007 - v3
Checksum:i1405021BDB5BA1AF
GO
Isoform b (identifier: P34547-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     32-43: Missing.

Note: No experimental confirmation available.

Show »
Length:414
Mass (Da):46,947
Checksum:i7D7D0C014C061398
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei32 – 4312Missing in isoform b. CuratedVSP_005268Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z29095 Genomic DNA. Translation: CAB54286.2.
Z29095 Genomic DNA. Translation: CAB54287.2.
PIRiS40715.
RefSeqiNP_499162.2. NM_066761.4. [P34547-1]
NP_499163.2. NM_066762.2. [P34547-2]
UniGeneiCel.6955.

Genome annotation databases

EnsemblMetazoaiR10E11.3a.1; R10E11.3a.1; WBGene00011216. [P34547-1]
R10E11.3a.2; R10E11.3a.2; WBGene00011216. [P34547-1]
GeneIDi176381.
KEGGicel:CELE_R10E11.3.
UCSCiR10E11.3a.1. c. elegans. [P34547-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z29095 Genomic DNA. Translation: CAB54286.2 .
Z29095 Genomic DNA. Translation: CAB54287.2 .
PIRi S40715.
RefSeqi NP_499162.2. NM_066761.4. [P34547-1 ]
NP_499163.2. NM_066762.2. [P34547-2 ]
UniGenei Cel.6955.

3D structure databases

ProteinModelPortali P34547.
SMRi P34547. Positions 26-406.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41576. 2 interactions.
STRINGi 6239.R10E11.3a.1.

Protein family/group databases

MEROPSi C19.A37.

Proteomic databases

PaxDbi P34547.
PRIDEi P34547.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai R10E11.3a.1 ; R10E11.3a.1 ; WBGene00011216 . [P34547-1 ]
R10E11.3a.2 ; R10E11.3a.2 ; WBGene00011216 . [P34547-1 ]
GeneIDi 176381.
KEGGi cel:CELE_R10E11.3.
UCSCi R10E11.3a.1. c. elegans. [P34547-1 ]

Organism-specific databases

CTDi 176381.
WormBasei R10E11.3a ; CE41150 ; WBGene00011216 ; usp-46.
R10E11.3b ; CE41151 ; WBGene00011216 ; usp-46.

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00650000093027.
HOGENOMi HOG000231498.
InParanoidi P34547.
KOi K11842.
OMAi DANIQMP.
PhylomeDBi P34547.

Miscellaneous databases

NextBioi 892334.
PROi P34547.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "The deubiquitinating enzyme USP-46 negatively regulates the degradation of glutamate receptors to control their abundance in the ventral nerve cord of Caenorhabditis elegans."
    Kowalski J.R., Dahlberg C.L., Juo P.
    J. Neurosci. 31:1341-1354(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GLR-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF CYS-36.
  4. "Deubiquitylation machinery is required for embryonic polarity in Caenorhabditis elegans."
    McCloskey R.J., Kemphues K.J.
    PLoS Genet. 8:E1003092-E1003092(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The WD40-repeat proteins WDR-20 and WDR-48 bind and activate the deubiquitinating enzyme USP-46 to promote the abundance of the glutamate receptor GLR-1 in the ventral nerve cord of Caenorhabditis elegans."
    Dahlberg C.L., Juo P.
    J. Biol. Chem. 289:3444-3456(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WDR-20 AND WDR-48, MUTAGENESIS OF CYS-36.

Entry informationi

Entry nameiUBP46_CAEEL
AccessioniPrimary (citable) accession number: P34547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: October 1, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3