P34547 (UBPX_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase 46
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme
Ubiquitin thioesterase
Ubiquitin-specific-processing protease

Gene names

Name:	usp-46
ORF Names:	R10E11.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from mutant phenotype PubMed 21273419. Source: WormBase regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Inferred from mutant phenotype PubMed 21273419. Source: WormBase
Cellular_component	cell body Inferred from direct assay PubMed 21273419. Source: WormBase endosome Inferred from direct assay PubMed 21273419. Source: WormBase
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P34547-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P34547-2)

The sequence of this isoform differs from the canonical sequence as follows:
     32-43: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 426	425	Probable ubiquitin carboxyl-terminal hydrolase 46		PRO_0000080684
Sites
	Active site	36	1	Nucleophile By similarity
	Active site	342	1	Proton acceptor By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
Natural variations
	Alternative sequence	32 – 43	12	Missing in isoform b.		VSP_005268

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 4, 2007. Version 3. Checksum: 1405021BDB5BA1AF	FASTA	426	48,277
	10 20 30 40 50 60 MGATGSSQLE KEISTTESVN NANEHYYGLV NFGNTCYCNS VIQALFFCRP FREKVLNYKQ 70 80 90 100 110 120 TLKKSGASKD NLVTCLADLF HSIASQKRRV GTIAPKRFIT KLKKENELFD NYMQQDAHEF 130 140 150 160 170 180 FNYLINTISE TLIQEKIAER EKASRHGTLK KGNVTVNLAP ATAGLPRSDE KGTSERNGGI 190 200 210 220 230 240 TVEGNEFLNK SDTTTWIHEI FQGILTNETR CLSCETVSSK DEDFLDLSID VEQNTSISHC 250 260 270 280 290 300 LRVFSETETL CGDQKYFCET CSSKQEAQKR MRIKKPPQLL ALHLKRFKFV EPLNRHTKLS 310 320 330 340 350 360 YRVVFPLELR LFNVSDDAEY GDRMYDLVAT VVHCGATPNR GHYITLVKSN SFWLVFDDDI 370 380 390 400 410 420 VEKLEVSSME EFSGMSTDAN IQMPPGNQSA PQKNSESAYI LFYQARDYAA DDPNHNHKGK NSTHSV
	Isoform b [UniParc]. Checksum: 7D7D0C014C061398	FASTA	414	46,947
	10 20 30 40 50 60 MGATGSSQLE KEISTTESVN NANEHYYGLV NALFFCRPFR EKVLNYKQTL KKSGASKDNL 70 80 90 100 110 120 VTCLADLFHS IASQKRRVGT IAPKRFITKL KKENELFDNY MQQDAHEFFN YLINTISETL 130 140 150 160 170 180 IQEKIAEREK ASRHGTLKKG NVTVNLAPAT AGLPRSDEKG TSERNGGITV EGNEFLNKSD 190 200 210 220 230 240 TTTWIHEIFQ GILTNETRCL SCETVSSKDE DFLDLSIDVE QNTSISHCLR VFSETETLCG 250 260 270 280 290 300 DQKYFCETCS SKQEAQKRMR IKKPPQLLAL HLKRFKFVEP LNRHTKLSYR VVFPLELRLF 310 320 330 340 350 360 NVSDDAEYGD RMYDLVATVV HCGATPNRGH YITLVKSNSF WLVFDDDIVE KLEVSSMEEF 370 380 390 400 410 SGMSTDANIQ MPPGNQSAPQ KNSESAYILF YQARDYAADD PNHNHKGKNS THSV

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z29095 Genomic DNA. Translation: CAB54286.2. Z29095 Genomic DNA. Translation: CAB54287.2.
PIR	S40715.
RefSeq	NP_499162.2. NM_066761.4. NP_499163.2. NM_066762.2.
UniGene	Cel.6955.
3D structure databases
ProteinModelPortal	P34547.
SMR	P34547. Positions 26-406.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.R10E11.3a.1.
Protein family/group databases
MEROPS	C19.A37.
Proteomic databases
PaxDb	P34547.
PRIDE	P34547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	R10E11.3a.1; R10E11.3a.1; R10E11.3. R10E11.3a.2; R10E11.3a.2; R10E11.3.
GeneID	176381.
KEGG	cel:CELE_R10E11.3.
UCSC	R10E11.3a.1. c. elegans.
Organism-specific databases
CTD	176381.
WormBase	R10E11.3a; CE41150; WBGene00011216; usp-46. R10E11.3b; CE41151; WBGene00011216; usp-46.
Phylogenomic databases
eggNOG	COG5077.
GeneTree	ENSGT00650000093027.
HOGENOM	HOG000231498.
InParanoid	P34547.
KO	K11842.
OMA	STDANIQ.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	892334.

Entry information

Entry name

UBPX_CAEEL

Accession

Primary (citable) accession number: P34547

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project