Reviewed, UniProtKB/Swiss-Prot P34544 (MET2_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable histone-lysine N-methyltransferase met-2
    EC=2.1.1.43

Gene names

Name:	met-2
ORF Names:	R05D3.11

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable histone methyltransferase.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. Contains 1 MBD (methyl-CpG-binding) domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: InterPro hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase negative regulation of vulval development Inferred from mutant phenotype. Source: WormBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1316	1316	Probable histone-lysine N-methyltransferase met-2		PRO_0000186066
Regions
	Domain	830 – 905	76	MBD
	Domain	967 – 1045	79	Pre-SET
	Domain	1063 – 1293	231	SET
	Domain	1298 – 1314	17	Post-SET
	Coiled coil	97 – 129	33	Potential

Sequences

	Sequence		Length	Mass (Da)
	P34544-1 [UniParc]. Last modified May 20, 2008. Version 3. Checksum: 77712B59478CACF3	FASTA	1,316	149,559
	10 20 30 40 50 60 MDQQEPSNNV DTSSILSDDG METQEQSSFV TATIDLTVDD YDETEIQEIL DNGKAEEGTD 70 80 90 100 110 120 EDSDLVEGIL NANSDVQALL DAPSEQVAQA LNSFFGNESE QEAVAAQRRV DAEKTAKDEA 130 140 150 160 170 180 ELKQQEEAED LIIEDSIVKT DEEKQAVRRL KINEFLSWFT RLLPEQFKNF EFTNPNYLTE 190 200 210 220 230 240 SISDSPVVNV DKCKEIVKSF KESESLEGLS QKYELIDEDV LVAAICIGVL DTNNEEDVDF 250 260 270 280 290 300 NVLCDDRIDD WSIEKCVTFL DYPNTGLNSK NGPLRFMQFT VTSPASAILM LTLIRLREEG 310 320 330 340 350 360 HPCRLDFDSN PTDDLLLNFD QVEFSNNIID TAVKYWDDQK ENGAQDKIGS FFHEIESTSA 370 380 390 400 410 420 EFKQHFENAV GSRNEIIQLV NEKIPDFDGT EAAVNESFTS DQRTEIINSR AIMETLKAEM 430 440 450 460 470 480 KLAIAEAQKV YDTKTDFEKF FVLTVGDFCL ARANPSDDAE LTYAIVQDRV DAMTYKVKFI 490 500 510 520 530 540 DTSQIRECNI RDLAMTTQGM YDPSLNTFGD VGLRVACRQV ISSSQFGKKT IWLTGTAAGR 550 560 570 580 590 600 RRAHRSDFLI FFDNGTDAYV SAPTMPGEPG YEVASEKKSV FSLKEMIAKM NAAQIAIMVG 610 620 630 640 650 660 QPVGKEGNLD YFLTFHWIRQ SHRSAYIRDF MKEFPEWPLL KMPVGMRICL YNSLVDRRKK 670 680 690 700 710 720 MVTVIGTDRA FAIVRHEAPN PLAPGNRCTD FPCNDRNHQH IDEKIYRGSH RLEGAAHKKH 730 740 750 760 770 780 MISTNNNLSQ RRKDQLQSQF EPTDMIRSMP ERNHQQVVKK KTTGTNQNVA STNDAKSKRE 790 800 810 820 830 840 IEIRKKNQFL FNKIIVPIPV LTPLENLKAH AQCGPDCLQK MDADPYEARF HRNSPIHTPL 850 860 870 880 890 900 LCGWRRIMYT MSTGKKRGAV KKNIIYFSPC GAALHQISDV SEYIHVTRSL LTIDCFSFDA 910 920 930 940 950 960 RIDTATYITV DDKYLKVADF SLGTEGIPIP LVNSVDNDEP PSLEYSKRRF QYNDQVDISS 970 980 990 1000 1010 1020 VSRDFCSGCS CDGDCSDASK CECQQLSIEA MKRLPHNLQF DGHDELVPHY QNRLLSSKVI 1030 1040 1050 1060 1070 1080 SGLYECNDQC SCHRKSCYNR VVQNNIKYPM HVSLFNDDTY QLLFFLQIFK TAQSGWGVRA 1090 1100 1110 1120 1130 1140 LTDIPQSTFI CTYVGAILTD DLADELRNAD QYFADLDLKD TVELEKGRED HETDFGYGGD 1150 1160 1170 1180 1190 1200 ESDYDDEEGS DGDSGDDVMN KMVKRQDSSE SGEETKRLTR QKRKQSKKSG KGGSVEKDDT 1210 1220 1230 1240 1250 1260 TPRDSMEKDN IESKDEPVFN WDKYFEPFPL YVIDAKQRGN LGRFLNHSCD PNVHVQHVMY 1270 1280 1290 1300 1310 DTHDLRLPWV AFFTRKYVKA GDELTWDYQY TQDQTATTQL TCHCGAENCT GRLLKS

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07144 Genomic DNA. Translation: AAK21437.2.
RefSeq	NP_498848.2.
UniGene	Cel.5325
3D structure databases
HSSP	HSSP built from PDB template 1ML9 based on UniProtKB Q8X225.
ModBase	Search...
Protein-protein interaction databases
STRING	P34544.
Genome annotation databases
Ensembl	R05D3.11; R05D3.11; R05D3.11; Caenorhabditis elegans. [Genome view]
GeneID	176183.
KEGG	cel:R05D3.11.
NMPDR	fig|6239.3.peg.10843.
UCSC	R05D3.11. c. elegans.
Organism-specific databases
CTD	176183.
WormBase	WBGene00019883. met-2.
WormPep	R05D3.11. CE42016. [WorfDB]
Enzyme and pathway databases
BRENDA	2.1.1.43. 672.
Gene expression databases
ArrayExpress	P34544.
Family and domain databases
InterPro	IPR001739. Methyl_CpG_DNA-bd. IPR003616. Post-SET_Zn_bd. IPR007728. Pre-SET_Zn_bd. IPR003606. Pre-SET_Zn_bd_sub. IPR001214. SET. [Graphical view]
Pfam	PF01429. MBD. 1 hit. PF05033. Pre-SET. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00391. MBD. 1 hit. SM00508. PostSET. 1 hit. SM00468. PreSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50982. MBD. 1 hit. PS50868. POST_SET. 1 hit. PS50867. PRE_SET. 1 hit. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891482.

Entry information

Entry name

MET2_CAEEL

Accession

Primary (citable) accession number: P34544
Secondary accession number(s): Q8WTP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 20, 2008
Last modified:	September 1, 2009
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project