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Protein

Kinesin heavy chain

Gene

unc-116

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport (By similarity). May play a role in endosome transport (PubMed:22634595).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 958ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: WormBase

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • establishment of meiotic spindle localization Source: WormBase
  • establishment of meiotic spindle orientation Source: WormBase
  • establishment or maintenance of microtubule cytoskeleton polarity Source: CACAO
  • locomotion Source: WormBase
  • meiotic cytokinesis Source: WormBase
  • microtubule-based movement Source: InterPro
  • polar body extrusion after meiotic divisions Source: WormBase
  • positive regulation of necrotic cell death Source: WormBase
  • regulation of dendrite morphogenesis Source: WormBase
  • synaptic vesicle transport Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin heavy chain
Alternative name(s):
Uncoordinated protein 116
Short name:
Protein unc-116
Gene namesi
Name:unc-116
Synonyms:khc-1
ORF Names:R05D3.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiR05D3.7; CE26945; WBGene00006840; unc-116.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • kinesin I complex Source: WormBase
  • microtubule Source: UniProtKB-KW
  • neuron projection Source: WormBase
  • nuclear envelope Source: WormBase
  • synapse Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in an altered distribution of recycling and late endosomes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Kinesin heavy chainPRO_0000125349Add
BLAST

Proteomic databases

EPDiP34540.
PaxDbiP34540.
PRIDEiP34540.

PTM databases

iPTMnetiP34540.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains.

Protein-protein interaction databases

BioGridi41383. 4 interactions.
IntActiP34540. 3 interactions.
MINTiMINT-1114807.
STRINGi6239.R05D3.7.

Structurei

3D structure databases

ProteinModelPortaliP34540.
SMRiP34540. Positions 8-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 329319Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili335 – 37440Sequence analysisAdd
BLAST
Coiled coili422 – 554133Sequence analysisAdd
BLAST
Coiled coili695 – 78591Sequence analysisAdd
BLAST

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000216718.
InParanoidiP34540.
KOiK10396.
OMAiGTMRENE.
PhylomeDBiP34540.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRTDGAEC GVQVFCRIRP LNKTEEKNAD RFLPKFPSED SISLGGKVYV
60 70 80 90 100
FDKVFKPNTT QEQVYKGAAY HIVQDVLSGY NGTVFAYGQT SSGKTHTMEG
110 120 130 140 150
VIGDNGLSGI IPRIVADIFN HIYSMDENLQ FHIKVSYYEI YNEKIRDLLD
160 170 180 190 200
PEKVNLSIHE DKNRVPYVKG ATERFVGGPD EVLQAIEDGK SNRMVAVTNM
210 220 230 240 250
NEHSSRSHSV FLITVKQEHQ TTKKQLTGKL YLVDLAGSEK VSKTGAQGTV
260 270 280 290 300
LEEAKNINKS LTALGIVISA LAEGTKSHVP YRDSKLTRIL QESLGGNSRT
310 320 330 340 350
TVIICASPSH FNEAETKSTL LFGARAKTIK NVVQINEELT AEEWKRRYEK
360 370 380 390 400
EKEKNTRLAA LLQAAALELS RWRAGESVSE VEWVNLSDSA QMAVSEVSGG
410 420 430 440 450
STPLMERSIA PAPPMLTSTT GPITDEEKKK YEEERVKLYQ QLDEKDDEIQ
460 470 480 490 500
KVSQELEKLR QQVLLQEEAL GTMRENEELI REENNRFQKE AEDKQQEGKE
510 520 530 540 550
MMTALEEIAV NLDVRQAECE KLKRELEVVQ EDNQSLEDRM NQATSLLNAH
560 570 580 590 600
LDECGPKIRH FKEGIYNVIR EFNIADIASQ NDQLPDHDLL NHVRIGVSKL
610 620 630 640 650
FSEYSAAKES STAAEHDAEA KLAADVARVE SGQDAGRMKQ LLVKDQAAKE
660 670 680 690 700
IKPLTDRVNM ELTTLKNLKK EFMRVLVARC QANQDTEGED SLSGPAQKQR
710 720 730 740 750
IQFLENNLDK LTKVHKQLVR DNADLRVELP KMEARLRGRE DRIKILETAL
760 770 780 790 800
RDSKQRSQAE RKKYQQEVER IKEAVRQRNM RRMNAPQIVK PIRPGQVYTS
810
PSAGMSQGAP NGSNA
Length:815
Mass (Da):91,894
Last modified:November 1, 1995 - v2
Checksum:i1B32718C3A7C254C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017163 mRNA. Translation: BAA32594.1.
L19120 Genomic DNA. Translation: AAA28155.1.
FO081667 Genomic DNA. Translation: CCD73193.1.
PIRiS44868.
RefSeqiNP_498842.1. NM_066441.6.
UniGeneiCel.37531.

Genome annotation databases

EnsemblMetazoaiR05D3.7; R05D3.7; WBGene00006840.
GeneIDi176179.
KEGGicel:CELE_R05D3.7.
UCSCiR05D3.7. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017163 mRNA. Translation: BAA32594.1.
L19120 Genomic DNA. Translation: AAA28155.1.
FO081667 Genomic DNA. Translation: CCD73193.1.
PIRiS44868.
RefSeqiNP_498842.1. NM_066441.6.
UniGeneiCel.37531.

3D structure databases

ProteinModelPortaliP34540.
SMRiP34540. Positions 8-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41383. 4 interactions.
IntActiP34540. 3 interactions.
MINTiMINT-1114807.
STRINGi6239.R05D3.7.

PTM databases

iPTMnetiP34540.

Proteomic databases

EPDiP34540.
PaxDbiP34540.
PRIDEiP34540.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR05D3.7; R05D3.7; WBGene00006840.
GeneIDi176179.
KEGGicel:CELE_R05D3.7.
UCSCiR05D3.7. c. elegans.

Organism-specific databases

CTDi176179.
WormBaseiR05D3.7; CE26945; WBGene00006840; unc-116.

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000216718.
InParanoidiP34540.
KOiK10396.
OMAiGTMRENE.
PhylomeDBiP34540.

Enzyme and pathway databases

ReactomeiR-CEL-983189. Kinesins.

Miscellaneous databases

NextBioi891464.
PROiP34540.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain."
    Patel N., Thierry-Mieg D., Mancillas J.R.
    Proc. Natl. Acad. Sci. U.S.A. 90:9181-9185(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. Siddiqui S.S., Ali M.Y., Khan M.A.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  5. "Caenorhabditis elegans screen reveals role of PAR-5 in RAB-11-recycling endosome positioning and apicobasal cell polarity."
    Winter J.F., Hoepfner S., Korn K., Farnung B.O., Bradshaw C.R., Marsico G., Volkmer M., Habermann B., Zerial M.
    Nat. Cell Biol. 14:666-676(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiKINH_CAEEL
AccessioniPrimary (citable) accession number: P34540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.