Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endoribonuclease dcr-1

Gene

dcr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. It produces 21 to 23 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs. Seems to process the precursor of the small temporal RNA let-7 which is involved in developmental timing.

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1682 – 16821Magnesium or manganeseBy similarity
Sitei1787 – 17871Important for activityBy similarity
Metal bindingi1791 – 17911Magnesium or manganeseBy similarity
Metal bindingi1794 – 17941Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 408ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • deoxyribonuclease I activity Source: WormBase
  • DNA binding Source: InterPro
  • helicase activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ribonuclease III activity Source: WormBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • apoptotic DNA fragmentation Source: WormBase
  • DNA catabolic process, endonucleolytic Source: WormBase
  • dsRNA fragmentation Source: WormBase
  • gonad morphogenesis Source: WormBase
  • nematode larval development Source: WormBase
  • olfactory learning Source: WormBase
  • positive regulation of primary miRNA processing Source: WormBase
  • pre-miRNA processing Source: WormBase
  • production of siRNA involved in RNA interference Source: WormBase
  • regulation of development, heterochronic Source: WormBase
  • reproduction Source: WormBase
  • RNA catabolic process Source: WormBase
  • RNA phosphodiester bond hydrolysis Source: GOC
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  • rRNA catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease dcr-1 (EC:3.1.26.-)
Gene namesi
Name:dcr-1
ORF Names:K12H4.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiK12H4.8; CE47418; WBGene00000939; dcr-1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • cytosol Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19101910Endoribonuclease dcr-1PRO_0000180473Add
BLAST

Proteomic databases

EPDiP34529.
PaxDbiP34529.
PRIDEiP34529.

Interactioni

Protein-protein interaction databases

BioGridi41344. 2 interactions.
DIPiDIP-25340N.
IntActiP34529. 1 interaction.
MINTiMINT-1040079.
STRINGi6239.K12H4.8.

Structurei

3D structure databases

ProteinModelPortaliP34529.
SMRiP34529. Positions 11-665, 713-1023, 1643-1894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 201182Helicase ATP-bindingAdd
BLAST
Domaini371 – 542172Helicase C-terminalAdd
BLAST
Domaini571 – 66797Dicer dsRNA-binding foldAdd
BLAST
Domaini847 – 1003157PAZAdd
BLAST
Domaini1381 – 1589209RNase III 1Add
BLAST
Domaini1643 – 1805163RNase III 2Add
BLAST
Domaini1833 – 189664DRBMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi145 – 1484DEAH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.Curated
Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated
Contains 1 PAZ domain.Curated
Contains 2 RNase III domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0701. Eukaryota.
COG0571. LUCA.
COG1111. LUCA.
GeneTreeiENSGT00510000046789.
HOGENOMiHOG000022395.
InParanoidiP34529.
KOiK11592.
OMAiVANMENM.
OrthoDBiEOG78PV82.

Family and domain databases

Gene3Di1.10.1520.10. 4 hits.
3.40.50.300. 2 hits.
HAMAPiMF_00104. RNase_III.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVRADLQC FNPRDYQVEL LDKATKKNTI VQLGTGSGKT FIAVLLLKEY
60 70 80 90 100
GVQLFAPLDQ GGKRAFFVVE KVNLVEQQAI HIEVHTSFKV GQVHGQTSSG
110 120 130 140 150
LWDSKEQCDQ FMKRHHVVVI TAQCLLDLIR HAYLKIEDMC VLIFDECHHA
160 170 180 190 200
LGSQHPYRSI MVDYKLLKKD KPVPRVLGLT ASLIKAKVAP EKLMEQLKKL
210 220 230 240 250
ESAMDSVIET ASDLVSLSKY GAKPYEVVII CKDFEIGCLG IPNFDTVIEI
260 270 280 290 300
FDETVAFVNT TTEFHPDLDL DPRRPIKDSL KTTRAVFRQL GPWAAWRTAQ
310 320 330 340 350
VWEKELGKII KSQVLPDKTL RFLNMAKTSM ITIKRLLEPE MKKIKSIEAL
360 370 380 390 400
RPYVPQRVIR LFEILETFNP EFQKERMKLE KAEHLSAIIF VDQRYIAYSL
410 420 430 440 450
LLMMRHIKSW EPKFKFVNPD YVVGASGRNL ASSDSQGLHK RQTEVLRRFH
460 470 480 490 500
RNEINCLIAT SVLEEGVDVK QCNLVIKFDR PLDMRSYVQS KGRARRAGSR
510 520 530 540 550
YVITVEEKDT AACDSDLKDF QQIEKILLSR HRTVNNPIED DSDRFEEFDV
560 570 580 590 600
DSQMEPYVVE KTGATLKMST AIALINRYCS KLPSDIFTRL VPHNQIIPIE
610 620 630 640 650
ENGVTKYCAE LLLPINSPIK HAIVLKNPMP NKKTAQMAVA LEACRQLHLE
660 670 680 690 700
GELDDNLLPK GRESIAKLLE HIDEEPDEYA PGIAAKVGSS KRKQLYDKKI
710 720 730 740 750
ARALNESFVE ADKECFIYAF ELERFREAEL TLNPKRRKFE DPFNYEYCFG
760 770 780 790 800
FLSAKEIPKI PPFPVFLRQG NMKVRLIVAP KKTTVTAAQL QEIQLFHNYL
810 820 830 840 850
FTQVLQMCKT GNLEFDGTSN APLNTLIVPL NKRKDDMSYT INMKYVSEVV
860 870 880 890 900
ANMENMPRIP KDEVRRQYKF NAEDYKDAIV MPWYRNLEQP VFYYVAEILP
910 920 930 940 950
EWRPSSKFPD THFETFNEYF IKKYKLEIYD QNQSLLDVDF TSTRLNLLQP
960 970 980 990 1000
RIQNQPRRSR TVSNSSTSNI PQASASDSKE SNTSVPHSSQ RQILVPELMD
1010 1020 1030 1040 1050
IHPISATLWN VIAALPSIFY RVNQLLLTDE LRETILVKAF GKEKTKLDDN
1060 1070 1080 1090 1100
VEWNSLAYAT EYEEKQTIIV KKIQQLRDLN QKSIEDQERE TRENDKIDDG
1110 1120 1130 1140 1150
EELFNIGVWD PEEAVRIGVE ISSRDDRMDG EDQDTVGLTQ GLHDGNISDE
1160 1170 1180 1190 1200
DDELPFVMHD YTARLTSNRN GIGAWSGSES IVPSGWGDWD GPEPDNSPMP
1210 1220 1230 1240 1250
FQILGGPGGL NVQALMADVG RVFDPSTASS SLSQTVQEST VSPPKQLTKE
1260 1270 1280 1290 1300
EEQFKKLQND LLKQAKERLE ALEMSEDMEK PRRLEDTVNL EDYGDDQENQ
1310 1320 1330 1340 1350
EDENTPTNFP KTIDEEIEEL SIGARKKQEI DDNAAKTDVL ERENCEVLPV
1360 1370 1380 1390 1400
AINEKSRSFS FEKESKAING RLIRQRSEEY VSHIDSDIGL GVSPCLLLTA
1410 1420 1430 1440 1450
LTTSNAADGM SLERFETIGD SFLKFATTDY LYHTLLDQHE GKLSFARSKE
1460 1470 1480 1490 1500
VSNCNLYRLG KKLGIPQLIV ANKFDAHDSW LPPCYIPTCD FKAPNTDDAE
1510 1520 1530 1540 1550
EKDNEIERIL DGQVIEEKPE NKTGWDIGGD VSKSTTDGIE TITFPKQARV
1560 1570 1580 1590 1600
GNDDISPLPY NLLTQQHISD KSIADAVEAL IGVHLLTLGP NPTLKVMNWM
1610 1620 1630 1640 1650
GLKVIQKDQK SDVPSPLLRF IDTPTNPNAS LNFLNNLWQQ FQFTQLEEKI
1660 1670 1680 1690 1700
GYRFKERAYL VQAFTHASYI NNRVTGCYQR LEFLGDAVLD YMITRYLFED
1710 1720 1730 1740 1750
SRQYSPGVLT DLRSALVNNT IFASLAVKFE FQKHFIAMCP GLYHMIEKFV
1760 1770 1780 1790 1800
KLCSERNFDT NFNAEMYMVT TEEEIDEGQE EDIEVPKAMG DIFESVAGAI
1810 1820 1830 1840 1850
YLDSGRNLDT TWQVIFHMMR GTIELCCANP PRSPIRELME FEQSKVRFSK
1860 1870 1880 1890 1900
MERILESGKV RVTVEVVNNM RFTGMGRNYR IAKATAAKRA LKYLHQIEQQ
1910
RRQSPSLTTV
Length:1,910
Mass (Da):218,423
Last modified:October 3, 2012 - v3
Checksum:iB49D19D7B3730F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081380 Genomic DNA. Translation: CCD71200.2.
PIRiS44849.
RefSeqiNP_498761.2. NM_066360.3.
UniGeneiCel.38691.

Genome annotation databases

EnsemblMetazoaiK12H4.8; K12H4.8; WBGene00000939.
GeneIDi176138.
KEGGicel:CELE_K12H4.8.
UCSCiK12H4.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081380 Genomic DNA. Translation: CCD71200.2.
PIRiS44849.
RefSeqiNP_498761.2. NM_066360.3.
UniGeneiCel.38691.

3D structure databases

ProteinModelPortaliP34529.
SMRiP34529. Positions 11-665, 713-1023, 1643-1894.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41344. 2 interactions.
DIPiDIP-25340N.
IntActiP34529. 1 interaction.
MINTiMINT-1040079.
STRINGi6239.K12H4.8.

Proteomic databases

EPDiP34529.
PaxDbiP34529.
PRIDEiP34529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK12H4.8; K12H4.8; WBGene00000939.
GeneIDi176138.
KEGGicel:CELE_K12H4.8.
UCSCiK12H4.8. c. elegans.

Organism-specific databases

CTDi42693.
WormBaseiK12H4.8; CE47418; WBGene00000939; dcr-1.

Phylogenomic databases

eggNOGiKOG0701. Eukaryota.
COG0571. LUCA.
COG1111. LUCA.
GeneTreeiENSGT00510000046789.
HOGENOMiHOG000022395.
InParanoidiP34529.
KOiK11592.
OMAiVANMENM.
OrthoDBiEOG78PV82.

Miscellaneous databases

NextBioi891290.
PROiP34529.

Family and domain databases

Gene3Di1.10.1520.10. 4 hits.
3.40.50.300. 2 hits.
HAMAPiMF_00104. RNase_III.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans."
    Ketting R.F., Fischer S.E.J., Bernstein E., Sijen T., Hannon G.J., Plasterk R.H.A.
    Genes Dev. 15:2654-2659(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiDCR1_CAEEL
AccessioniPrimary (citable) accession number: P34529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 3, 2012
Last modified: May 11, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.