Reviewed, UniProtKB/Swiss-Prot P34517 (GPDA_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
    EC=1.1.1.8

Gene names

ORF Names:

K11H3.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Probable glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic		PRO_0000138070
Regions
	Nucleotide binding	42 – 47	6	NAD By similarity
	Region	312 – 313	2	Substrate binding By similarity
Sites
	Active site	248	1	Proton acceptor By similarity
	Binding site	130	1	NAD By similarity
	Binding site	153	1	NAD; via amide nitrogen By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	196	1	NAD; via amide nitrogen By similarity
	Binding site	312	1	NAD By similarity
	Binding site	341	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P34517-1 [UniParc]. Last modified May 16, 2003. Version 2. Checksum: E42BCCBE280B04A5	FASTA	392	42,792
	10 20 30 40 50 60 MSSSRIPQCE NSSRLSLSSI FRYFGTTSTI ATMSPKKVTI IGSGNWGSAI ARIVGSTTKS 70 80 90 100 110 120 FPDEFDPTVR MWVFEEIVNG EKLSEVINNR HENIKYLPGK VLPNNVVAVT DLVESCEGSN 130 140 150 160 170 180 VLVFVVPHQF VKGICEKLVG KIPADTQAIS LIKGISFDKT NQGVSTEKRG GLKLISEEIK 190 200 210 220 230 240 EILKIEVSVL MGANLAPEVA NDNFCEATIG CKRKAEDGPL LKKLFHTDNF RINVVEDAHT 250 260 270 280 290 300 VELCGALKNV VACAAGFTDG LGYGDNTKAA VIRLGLMETT KFVEHYYPGS NLQTFFESCG 310 320 330 340 350 360 IADLITTCYG GRNRKVCEAF VKTGKSMAEV EKELLNGQSA QGPLTAEEVY LMMHKTGLDA 370 380 390 KFPLFTAVHK ICAGEMKPAE LVDCLRNHPE HM

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22180 Genomic DNA. Translation: CAD54146.1.
PIR	S40754.
RefSeq	NP_871632.1.
UniGene	Cel.10288
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P34517. 2 interactions.
STRING	P34517.
Proteomic databases
PRIDE	P34517.
Genome annotation databases
Ensembl	K11H3.1b; K11H3.1b; K11H3.1; Caenorhabditis elegans. [Genome view]
GeneID	176399.
Organism-specific databases
CTD	176399.
WormBase	WBGene00010778. K11H3.1.
WormPep	K11H3.1b. CE31851. [WorfDB]
Phylogenomic databases
OMA	THENVKY.
Enzyme and pathway databases
BRENDA	1.1.1.8. 672.
Gene expression databases
ArrayExpress	P34517.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	892406.

Entry information

Entry name

GPDA_CAEEL

Accession

Primary (citable) accession number: P34517
Secondary accession number(s): Q8I4H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 16, 2003
Last modified:	November 3, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project