P34477 (UBC7_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 7
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 7
Ubiquitin-protein ligase 7

Gene names

Name:	ubc-7
ORF Names:	F58A4.10

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 164

164

Probable ubiquitin-conjugating enzyme E2 7

PRO_0000082516

Sites

Active site

88

1

Glycyl thioester intermediate By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

164

Helix Strand Turn

Details...

Helix

5 – 17

13

Beta strand

23 – 29

7

Beta strand

35 – 41

7

Beta strand

52 – 58

7

Helix

63 – 65

3

Beta strand

69 – 72

4

Beta strand

85 – 87

3

Helix

90 – 92

3

Helix

115 – 127

13

Helix

137 – 144

8

Helix

147 – 161

15

Sequences

	Sequence		Length	Mass (Da)
	P34477 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 62580D9F3F8FD968	FASTA	164	18,939
	10 20 30 40 50 60 MEQSSLLLKK QLADMRRVPV DGFSAGLVDD NDIYKWEVLV IGPPDTLYEG GFFKAILDFP 70 80 90 100 110 120 RDYPQKPPKM KFISEIWHPN IDKEGNVCIS ILHDPGDDKW GYERPEERWL PVHTVETILL 130 140 150 160 SVISMLTDPN FESPANVDAA KMQRENYAEF KKKVAQCVRR SQEE

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z22179 Genomic DNA. Translation: CAA80166.1.

PIR

S40982.

RefSeq

NP_499133.1. NM_066732.3.

UniGene

Cel.18114.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PZV	X-ray	2.52	A	1-164	[»]

ProteinModelPortal

P34477.

SMR

P34477. Positions 4-164.

ModBase

Search...

Protein-protein interaction databases

STRING

P34477.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

F58A4.10.1; F58A4.10.1; F58A4.10.
F58A4.10.2; F58A4.10.2; F58A4.10.

GeneID

176363.

KEGG

cel:F58A4.10.

UCSC

F58A4.10.1. c. elegans.

Organism-specific databases

CTD

176363.

WormBase

F58A4.10; CE00216; WBGene00006704; ubc-7.

Phylogenomic databases

eggNOG

meNOG06697.

GeneTree

EMGT00050000007020.

HOGENOM

HBG756483.

InParanoid

P34477.

OMA

FISEIWH.

PhylomeDB

P34477.

Gene expression databases

ArrayExpress

P34477.

Family and domain databases

InterPro

IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

KO

K10575.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 1 hit.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

892258.

Entry information

Entry name

UBC7_CAEEL

Accession

Primary (citable) accession number: P34477

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project