P34477 (UBC7_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 7
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 7
Ubiquitin-protein ligase 7

Gene names

Name:	ubc-7
ORF Names:	F58A4.10

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 164

164

Probable ubiquitin-conjugating enzyme E2 7

PRO_0000082516

Sites

Active site

88

1

Glycyl thioester intermediate By similarity

Secondary structure

1

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164

Helix Strand Turn

Details...

Helix

5 – 17

13

Beta strand

23 – 29

7

Beta strand

35 – 41

7

Beta strand

52 – 58

7

Helix

63 – 65

3

Beta strand

69 – 72

4

Beta strand

85 – 87

3

Helix

90 – 92

3

Helix

115 – 127

13

Helix

137 – 144

8

Helix

147 – 161

15

Sequences

	Sequence		Length	Mass (Da)
	P34477 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 62580D9F3F8FD968	FASTA	164	18,939
	10 20 30 40 50 60 MEQSSLLLKK QLADMRRVPV DGFSAGLVDD NDIYKWEVLV IGPPDTLYEG GFFKAILDFP 70 80 90 100 110 120 RDYPQKPPKM KFISEIWHPN IDKEGNVCIS ILHDPGDDKW GYERPEERWL PVHTVETILL 130 140 150 160 SVISMLTDPN FESPANVDAA KMQRENYAEF KKKVAQCVRR SQEE

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z22179 Genomic DNA. Translation: CAA80166.1.

PIR

S40982.

RefSeq

NP_499133.1. NM_066732.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PZV	X-ray	2.52	A	1-164	[»]

ProteinModelPortal

P34477.

SMR

P34477. Positions 4-164.

ModBase

Search...

Protein-protein interaction databases

STRING

6239.F58A4.10.1.

Proteomic databases

PaxDb

P34477.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

F58A4.10.1; F58A4.10.1; F58A4.10.
F58A4.10.2; F58A4.10.2; F58A4.10.

GeneID

176363.

KEGG

cel:CELE_F58A4.10.

UCSC

F58A4.10.1. c. elegans.

Organism-specific databases

CTD

176363.

WormBase

F58A4.10; CE00216; WBGene00006704; ubc-7.

Phylogenomic databases

eggNOG

COG5078.

GeneTree

ENSGT00530000063258.

HOGENOM

HOG000233454.

InParanoid

P34477.

KO

K10575.

OMA

EWREDRH.

Enzyme and pathway databases

UniPathway

UPA00143.

Family and domain databases

Gene3D

3.10.110.10. 1 hit.

InterPro

IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 1 hit.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P34477.

NextBio

892258.

Entry information

Entry name

UBC7_CAEEL

Accession

Primary (citable) accession number: P34477

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project