Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable aconitate hydratase, mitochondrial

Gene

aco-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.By similarity

Catalytic activityi

Citrate = isocitrate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable citrate synthase, mitochondrial (cts-1)
  2. Probable aconitate hydratase, mitochondrial (aco-2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Metal bindingi382 – 3821Iron-sulfur (4Fe-4S)By similarity
Metal bindingi445 – 4451Iron-sulfur (4Fe-4S)By similarity
Metal bindingi448 – 4481Iron-sulfur (4Fe-4S)By similarity
Binding sitei471 – 4711SubstrateBy similarity
Binding sitei476 – 4761SubstrateBy similarity
Binding sitei601 – 6011SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00718.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aconitate hydratase, mitochondrial (EC:4.2.1.3)
Short name:
Aconitase
Alternative name(s):
Citrate hydro-lyase
Gene namesi
Name:aco-2
ORF Names:F54H12.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF54H12.1a; CE25005; WBGene00000041; aco-2.
F54H12.1b; CE30144; WBGene00000041; aco-2.
F54H12.1c; CE32436; WBGene00000041; aco-2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionSequence analysisAdd
BLAST
Chaini27 – 777751Probable aconitate hydratase, mitochondrialPRO_0000000545Add
BLAST

Proteomic databases

EPDiP34455.
PaxDbiP34455.
PRIDEiP34455.

2D gel databases

World-2DPAGE0020:P34455.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi6239.F54H12.1a.

Structurei

3D structure databases

ProteinModelPortaliP34455.
SMRiP34455. Positions 27-775.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1913Substrate bindingBy similarity
Regioni664 – 6652Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0453. Eukaryota.
COG1048. LUCA.
GeneTreeiENSGT00840000129913.
HOGENOMiHOG000224293.
InParanoidiP34455.
KOiK01681.
OMAiPLKCIIK.
OrthoDBiEOG74FF06.
PhylomeDBiP34455.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P34455-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSLLRLSHL AGPAHYRALH SSSSIWSKVA ISKFEPKSYL PYEKLSQTVK
60 70 80 90 100
IVKDRLKRPL TLSEKILYGH LDQPKTQDIE RGVSYLRLRP DRVAMQDATA
110 120 130 140 150
QMAMLQFISS GLPKTAVPST IHCDHLIEAQ KGGAQDLARA KDLNKEVFNF
160 170 180 190 200
LATAGSKYGV GFWKPGSGII HQIILENYAF PGLLLIGTDS HTPNGGGLGG
210 220 230 240 250
LCIGVGGADA VDVMADIPWE LKCPKVIGIK LTGKLNGWTS AKDVILKVAD
260 270 280 290 300
ILTVKGGTGA IVEYFGPGVD SISATGMGTI CNMGAEIGAT TSVFPYNESM
310 320 330 340 350
YKYLEATGRK EIAEEARKYK DLLTADDGAN YDQIIEINLD TLTPHVNGPF
360 370 380 390 400
TPDLASSIDK LGENAKKNGW PLDVKVSLIG SCTNSSYEDM TRAASIAKQA
410 420 430 440 450
LDKGLKAKTI FTITPGSEQV RATIERDGLS KIFADFGGMV LANACGPCIG
460 470 480 490 500
QWDRQDVKKG EKNTIVTSYN RNFTGRNDAN PATHGFVTSP DITTAMAISG
510 520 530 540 550
RLDFNPLTDE LTAADGSKFK LQAPTGLDLP PKGYDPGEDT FQAPSGSGQV
560 570 580 590 600
DVSPSSDRLQ LLSPFDKWDG KDLEDMKILI KVTGKCTTDH ISAAGPWLKY
610 620 630 640 650
RGHLDNISNN LFLTAINADN GEMNKVKNQV TGEYGAVPAT ARKYKADGVR
660 670 680 690 700
WVAIGDENYG EGSSREHAAL EPRHLGGRAI IVKSFARIHE TNLKKQGMLP
710 720 730 740 750
LTFANPADYD KIDPSDNVSI VGLSSFAPGK PLTAIFKKTN GSKVEVTLNH
760 770
TFNEQQIEWF KAGSALNRMK EVFAKSK
Note: No experimental confirmation available.
Length:777
Mass (Da):84,047
Last modified:May 27, 2002 - v2
Checksum:iD482AAD48BAB0844
GO
Isoform b (identifier: P34455-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-208: Missing.

Note: No experimental confirmation available.
Show »
Length:665
Mass (Da):72,528
Checksum:i81DF1D0AA488EB95
GO
Isoform c (identifier: P34455-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.
Show »
Length:683
Mass (Da):73,236
Checksum:i2193B2223EE175E1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9494Missing in isoform c. CuratedVSP_016115Add
BLAST
Alternative sequencei97 – 208112Missing in isoform b. CuratedVSP_016116Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080401 Genomic DNA. Translation: CCD63454.1.
FO080401 Genomic DNA. Translation: CCD63455.1.
FO080401 Genomic DNA. Translation: CCD63456.1.
PIRiS44831.
RefSeqiNP_498738.2. NM_066337.5. [P34455-3]
NP_741235.1. NM_171200.4. [P34455-1]
NP_741236.1. NM_171885.1. [P34455-2]
UniGeneiCel.17858.

Genome annotation databases

EnsemblMetazoaiF54H12.1a; F54H12.1a; WBGene00000041. [P34455-1]
GeneIDi176121.
KEGGicel:CELE_F54H12.1.
UCSCiF54H12.1c.2. c. elegans. [P34455-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080401 Genomic DNA. Translation: CCD63454.1.
FO080401 Genomic DNA. Translation: CCD63455.1.
FO080401 Genomic DNA. Translation: CCD63456.1.
PIRiS44831.
RefSeqiNP_498738.2. NM_066337.5. [P34455-3]
NP_741235.1. NM_171200.4. [P34455-1]
NP_741236.1. NM_171885.1. [P34455-2]
UniGeneiCel.17858.

3D structure databases

ProteinModelPortaliP34455.
SMRiP34455. Positions 27-775.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F54H12.1a.

2D gel databases

World-2DPAGE0020:P34455.

Proteomic databases

EPDiP34455.
PaxDbiP34455.
PRIDEiP34455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF54H12.1a; F54H12.1a; WBGene00000041. [P34455-1]
GeneIDi176121.
KEGGicel:CELE_F54H12.1.
UCSCiF54H12.1c.2. c. elegans. [P34455-1]

Organism-specific databases

CTDi176121.
WormBaseiF54H12.1a; CE25005; WBGene00000041; aco-2.
F54H12.1b; CE30144; WBGene00000041; aco-2.
F54H12.1c; CE32436; WBGene00000041; aco-2.

Phylogenomic databases

eggNOGiKOG0453. Eukaryota.
COG1048. LUCA.
GeneTreeiENSGT00840000129913.
HOGENOMiHOG000224293.
InParanoidiP34455.
KOiK01681.
OMAiPLKCIIK.
OrthoDBiEOG74FF06.
PhylomeDBiP34455.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
ReactomeiR-CEL-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi891200.
PROiP34455.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiACON_CAEEL
AccessioniPrimary (citable) accession number: P34455
Secondary accession number(s): Q8IG17, Q8WQF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 27, 2002
Last modified: May 11, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.