P34455 (ACON_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aconitate hydratase, mitochondrial
Short name=Aconitase
EC=4.2.1.3

Alternative name(s):
Citrate hydro-lyase

Gene names

Name:	aco-2
ORF Names:	F54H12.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA interference Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P34455-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: P34455-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-208: Missing.

Note: No experimental confirmation available.

Isoform c (identifier: P34455-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 777	751	Probable aconitate hydratase, mitochondrial		PRO_0000000545
Regions
	Region	189 – 191	3	Substrate binding By similarity
	Region	664 – 665	2	Substrate binding By similarity
Sites
	Metal binding	382	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	445	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	448	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	471	1	Substrate By similarity
	Binding site	476	1	Substrate By similarity
	Binding site	601	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 94	94	Missing in isoform c.		VSP_016115
	Alternative sequence	97 – 208	112	Missing in isoform b.		VSP_016116

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified May 27, 2002. Version 2. Checksum: D482AAD48BAB0844	FASTA	777	84,047
	10 20 30 40 50 60 MNSLLRLSHL AGPAHYRALH SSSSIWSKVA ISKFEPKSYL PYEKLSQTVK IVKDRLKRPL 70 80 90 100 110 120 TLSEKILYGH LDQPKTQDIE RGVSYLRLRP DRVAMQDATA QMAMLQFISS GLPKTAVPST 130 140 150 160 170 180 IHCDHLIEAQ KGGAQDLARA KDLNKEVFNF LATAGSKYGV GFWKPGSGII HQIILENYAF 190 200 210 220 230 240 PGLLLIGTDS HTPNGGGLGG LCIGVGGADA VDVMADIPWE LKCPKVIGIK LTGKLNGWTS 250 260 270 280 290 300 AKDVILKVAD ILTVKGGTGA IVEYFGPGVD SISATGMGTI CNMGAEIGAT TSVFPYNESM 310 320 330 340 350 360 YKYLEATGRK EIAEEARKYK DLLTADDGAN YDQIIEINLD TLTPHVNGPF TPDLASSIDK 370 380 390 400 410 420 LGENAKKNGW PLDVKVSLIG SCTNSSYEDM TRAASIAKQA LDKGLKAKTI FTITPGSEQV 430 440 450 460 470 480 RATIERDGLS KIFADFGGMV LANACGPCIG QWDRQDVKKG EKNTIVTSYN RNFTGRNDAN 490 500 510 520 530 540 PATHGFVTSP DITTAMAISG RLDFNPLTDE LTAADGSKFK LQAPTGLDLP PKGYDPGEDT 550 560 570 580 590 600 FQAPSGSGQV DVSPSSDRLQ LLSPFDKWDG KDLEDMKILI KVTGKCTTDH ISAAGPWLKY 610 620 630 640 650 660 RGHLDNISNN LFLTAINADN GEMNKVKNQV TGEYGAVPAT ARKYKADGVR WVAIGDENYG 670 680 690 700 710 720 EGSSREHAAL EPRHLGGRAI IVKSFARIHE TNLKKQGMLP LTFANPADYD KIDPSDNVSI 730 740 750 760 770 VGLSSFAPGK PLTAIFKKTN GSKVEVTLNH TFNEQQIEWF KAGSALNRMK EVFAKSK
	Isoform b [UniParc]. Checksum: 81DF1D0AA488EB95	FASTA	665	72,528
	10 20 30 40 50 60 MNSLLRLSHL AGPAHYRALH SSSSIWSKVA ISKFEPKSYL PYEKLSQTVK IVKDRLKRPL 70 80 90 100 110 120 TLSEKILYGH LDQPKTQDIE RGVSYLRLRP DRVAMQDAVD VMADIPWELK CPKVIGIKLT 130 140 150 160 170 180 GKLNGWTSAK DVILKVADIL TVKGGTGAIV EYFGPGVDSI SATGMGTICN MGAEIGATTS 190 200 210 220 230 240 VFPYNESMYK YLEATGRKEI AEEARKYKDL LTADDGANYD QIIEINLDTL TPHVNGPFTP 250 260 270 280 290 300 DLASSIDKLG ENAKKNGWPL DVKVSLIGSC TNSSYEDMTR AASIAKQALD KGLKAKTIFT 310 320 330 340 350 360 ITPGSEQVRA TIERDGLSKI FADFGGMVLA NACGPCIGQW DRQDVKKGEK NTIVTSYNRN 370 380 390 400 410 420 FTGRNDANPA THGFVTSPDI TTAMAISGRL DFNPLTDELT AADGSKFKLQ APTGLDLPPK 430 440 450 460 470 480 GYDPGEDTFQ APSGSGQVDV SPSSDRLQLL SPFDKWDGKD LEDMKILIKV TGKCTTDHIS 490 500 510 520 530 540 AAGPWLKYRG HLDNISNNLF LTAINADNGE MNKVKNQVTG EYGAVPATAR KYKADGVRWV 550 560 570 580 590 600 AIGDENYGEG SSREHAALEP RHLGGRAIIV KSFARIHETN LKKQGMLPLT FANPADYDKI 610 620 630 640 650 660 DPSDNVSIVG LSSFAPGKPL TAIFKKTNGS KVEVTLNHTF NEQQIEWFKA GSALNRMKEV FAKSK
	Isoform c [UniParc]. Checksum: 2193B2223EE175E1	FASTA	683	73,236
	10 20 30 40 50 60 MQDATAQMAM LQFISSGLPK TAVPSTIHCD HLIEAQKGGA QDLARAKDLN KEVFNFLATA 70 80 90 100 110 120 GSKYGVGFWK PGSGIIHQII LENYAFPGLL LIGTDSHTPN GGGLGGLCIG VGGADAVDVM 130 140 150 160 170 180 ADIPWELKCP KVIGIKLTGK LNGWTSAKDV ILKVADILTV KGGTGAIVEY FGPGVDSISA 190 200 210 220 230 240 TGMGTICNMG AEIGATTSVF PYNESMYKYL EATGRKEIAE EARKYKDLLT ADDGANYDQI 250 260 270 280 290 300 IEINLDTLTP HVNGPFTPDL ASSIDKLGEN AKKNGWPLDV KVSLIGSCTN SSYEDMTRAA 310 320 330 340 350 360 SIAKQALDKG LKAKTIFTIT PGSEQVRATI ERDGLSKIFA DFGGMVLANA CGPCIGQWDR 370 380 390 400 410 420 QDVKKGEKNT IVTSYNRNFT GRNDANPATH GFVTSPDITT AMAISGRLDF NPLTDELTAA 430 440 450 460 470 480 DGSKFKLQAP TGLDLPPKGY DPGEDTFQAP SGSGQVDVSP SSDRLQLLSP FDKWDGKDLE 490 500 510 520 530 540 DMKILIKVTG KCTTDHISAA GPWLKYRGHL DNISNNLFLT AINADNGEMN KVKNQVTGEY 550 560 570 580 590 600 GAVPATARKY KADGVRWVAI GDENYGEGSS REHAALEPRH LGGRAIIVKS FARIHETNLK 610 620 630 640 650 660 KQGMLPLTFA NPADYDKIDP SDNVSIVGLS SFAPGKPLTA IFKKTNGSKV EVTLNHTFNE 670 680 QQIEWFKAGS ALNRMKEVFA KSK

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080401 Genomic DNA. Translation: CCD63454.1. FO080401 Genomic DNA. Translation: CCD63455.1. FO080401 Genomic DNA. Translation: CCD63456.1.
PIR	S44831.
RefSeq	NP_498738.2. NM_066337.4. NP_741235.1. NM_171200.3. NP_741236.1. NM_171885.1.
UniGene	Cel.17858.
3D structure databases
ProteinModelPortal	P34455.
SMR	P34455. Positions 27-775.
ModBase	Search...
Protein-protein interaction databases
IntAct	P34455. 4 interactions.
STRING	P34455.
2D gel databases
World-2DPAGE	0020:P34455.
Proteomic databases
PRIDE	P34455.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F54H12.1a; F54H12.1a; F54H12.1.
GeneID	176121.
KEGG	cel:F54H12.1.
UCSC	F54H12.1c.2. c. elegans.
Organism-specific databases
CTD	176121.
WormBase	F54H12.1a; CE25005; WBGene00000041; aco-2. F54H12.1b; CE30144; WBGene00000041; aco-2. F54H12.1c; CE32436; WBGene00000041; aco-2.
Phylogenomic databases
eggNOG	meNOG04827.
GeneTree	EMGT00050000006872.
HOGENOM	HBG295973.
InParanoid	P34455.
OMA	KHGIRWV.
PhylomeDB	P34455.
Gene expression databases
ArrayExpress	P34455.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR006248. Aconitase_mito-like. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
KO	K01681.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF5. Aconitase_mito. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR01340. Aconitase_mito. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	891200.

Entry information

Entry name

ACON_CAEEL

Accession

Primary (citable) accession number: P34455
Secondary accession number(s): Q8IG17, Q8WQF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 27, 2002
Last modified:	December 14, 2011
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project