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Protein

Integrin alpha pat-2

Gene

pat-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for muscle development probably through the regulation of the actin-myosin cytoskeleton (PubMed:8106547, PubMed:12915588). Component of an integrin containing attachment complex, which is required for muscle maintenance (PubMed:22253611). During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles, probably through lamins such as epi-1, lam-2 and unc-52 (PubMed:16495308). Required for distal tip cell migration and dorsal pathfinding (PubMed:17606640). Required for egg-laying (PubMed:12915588). May play a role in cell motility and cell-cell interactions (By similarity). Plays a role in vulval development (PubMed:28135265). Probably within the alpha pat-2/beta pat-3 integrin receptor complex, plays a role in the negative regulation of let-23 signaling and vulval induction. This is probably partly by restricting the mobility of the let-23 receptor on the plasma membrane of vulval cells which thereby attenuates let-23 signaling (PubMed:28135265).By similarity6 Publications

GO - Molecular functioni

  • transmembrane signaling receptor activity Source: WormBase

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • mitochondrion organization Source: UniProtKB
  • muscle cell cellular homeostasis Source: UniProtKB
  • negative regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
  • positive regulation of engulfment of apoptotic cell Source: WormBase
  • positive regulation of locomotion Source: UniProtKB
  • positive regulation of sarcomere organization Source: UniProtKB
  • regulation of vulval development Source: UniProtKB
  • vulval cell fate specification Source: UniProtKB

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-CEL-354192 Integrin alphaIIb beta3 signaling
R-CEL-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-CEL-6798695 Neutrophil degranulation
SignaLinkiP34446

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha pat-2
Alternative name(s):
Paralyzed arrest at two-fold protein 2
Gene namesi
Name:pat-2Imported
ORF Names:F54F2.1Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi

Organism-specific databases

WormBaseiF54F2.1 ; CE00194 ; WBGene00003929 ; pat-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 1154ExtracellularSequence analysisAdd BLAST1129
Transmembranei1155 – 1177HelicalSequence analysisAdd BLAST23
Topological domaini1178 – 1226CytoplasmicSequence analysisAdd BLAST49

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Severe paralysis at the 1-fold stage of embryonic development followed by an arrest in elongation at 2-fold stage (PubMed:8106547). Loss of myosin and actin organization in embryonic body wall muscles and loss of muscle cell polarization (PubMed:8106547). RNAi-mediated knockdown in post-hatching animals causes paralysis associated with severe disorganization of body wall muscle actin filaments and defects in egg-laying associated with embryonic hatching within the mother (the bag of worms phenotype) (PubMed:12915588). Few surviving adults, are uncoordinated with abnormal body size and shape and have defects in distal tip cells (DTC) migration resulting in abnormal gonad formation (PubMed:17606640). RNAi-mediated knockdown in L4 larval stage, causes ectopic membrane extensions from body wall muscles (PubMed:16495308). RNAi-mediated knockdown results in impaired mobility, mitochondrial fragmentation and disrupted integrin attachment complexes in muscle (PubMed:22253611). This leads to degradation of muscle proteins in the cytosol, myofibrillar defects and disruption of sarcomere organization (PubMed:22253611). RNAi-mediated knockdown in vulval precursor cells in a let-60 gain of function mutant background results in increased vulval induction and an adjacent primary fate (Apf) phenotype whereby secondary vulval precursor cells transform into primary-like vulval cells (PubMed:28135265).6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi441G → D in st567; severe paralysis at embryonic 1-fold stage followed by an arrest in elongation at 2-fold stage. Loss of myosin and actin organization in embryonic muscles. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001633226 – 1226Integrin alpha pat-2Add BLAST1201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi108N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi228N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi290N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi608N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi679N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi775N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi819N-linked (GlcNAc...) asparagine2 Publications1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP34446
PaxDbiP34446
PeptideAtlasiP34446
PRIDEiP34446

PTM databases

iPTMnetiP34446

Expressioni

Tissue specificityi

Expressed in body-wall muscle cells, distal tip cells, and vulval tissue.1 Publication

Developmental stagei

Up-regulated during L3 developmental stage in distal tip cells.1 Publication

Gene expression databases

BgeeiWBGene00003929

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit (Probable). Interacts with beta subunit pat-3 (Probable). Interacts with dep-1 (PubMed:28135265). Component of an integrin containing attachment complex, composed of at least pat-2, pat-3, pat-4, pat-6, unc-52, unc-97 and unc-112 (PubMed:22253611).Curated1 Publication

Protein-protein interaction databases

BioGridi41442, 2 interactors
STRINGi6239.F54F2.1

Structurei

3D structure databases

ProteinModelPortaliP34446
SMRiP34446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 94FG-GAP 1PROSITE-ProRule annotationAdd BLAST68
Repeati108 – 171FG-GAP 2PROSITE-ProRule annotationAdd BLAST64
Repeati178 – 233FG-GAP 3PROSITE-ProRule annotationAdd BLAST56
Repeati234 – 290FG-GAP 4PROSITE-ProRule annotationAdd BLAST57
Repeati291 – 345FG-GAP 5PROSITE-ProRule annotationAdd BLAST55
Repeati362 – 421FG-GAP 6PROSITE-ProRule annotationAdd BLAST60
Repeati425 – 488FG-GAP 7PROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi620 – 622Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3637 Eukaryota
ENOG410XPVZ LUCA
GeneTreeiENSGT00760000118782
HOGENOMiHOG000115457
InParanoidiP34446
OMAiESNPREV
OrthoDBiEOG091G02JI
PhylomeDBiP34446

Family and domain databases

Gene3Di2.130.10.130, 1 hit
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR013519 Int_alpha_beta-p
IPR000413 Integrin_alpha
IPR013649 Integrin_alpha-2
IPR018184 Integrin_alpha_C_CS
IPR028994 Integrin_alpha_N
IPR032695 Integrin_dom_sf
PfamiView protein in Pfam
PF01839 FG-GAP, 1 hit
PF00357 Integrin_alpha, 1 hit
PF08441 Integrin_alpha2, 1 hit
PRINTSiPR01185 INTEGRINA
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
SUPFAMiSSF69179 SSF69179, 4 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits
PS00242 INTEGRIN_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P34446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREGSFPRRI GLLLGLLGLL AGVATFNIDT KNVVVHHMAG NYFGYSLDFY
60 70 80 90 100
HEQKGMPVLV VGAPEAESNN PNLAGIRRPG AVYACSVNRA TCREVHVDKM
110 120 130 140 150
KGNLKKLNGS HLVPIEEKSH QFFGATVRSN DKHDKIVVCA PKYTYFYSKF
160 170 180 190 200
EVIEPVGTCF YAENGFDNAE EFSSCKQEPA RHGRHRLGYG QCGFSAAVPG
210 220 230 240 250
KKNQNRVFIG APGVWYWQGA MFSQNIKNQT DRPNTEYGSK EYDHDMMGYS
260 270 280 290 300
TATGDFDGDG IDDIVAGVPR GNDLHGKLVL YTSKLKMMIN LTDEVSTQHG
310 320 330 340 350
QYCGGSVAVA DVNKDGRDDI IMGCPFYTDY GSVKDAKTQE RKPQYDVGKV
360 370 380 390 400
IVMLQTAPGV FGKQIAVVGD DQWGRFGHAV AAAGDLNLDG YNDVIVGAPY
410 420 430 440 450
AGKNKQGAVY VIHGSKDGVR ELPTQKIEGA NIGHGNIKSF GFSLTGNEDV
460 470 480 490 500
DGNGMPDIAV GAWKSGNAAV LLTKPVVTVT GQTEPESALI SVEDKNCDVD
510 520 530 540 550
GKLGKQACKH INTCFKYEGK GDTPNDLEFD LRFNLDDHSP EPRAYFLQKD
560 570 580 590 600
VKSDRSIKVA QGSKTRDHPS SIEQRVRLEK GRQKCFRHRF FASSTMKDKL
610 620 630 640 650
SPIHWSVNYT YVESKTGKLR GDKLEPAIDT TVPLSFQNKI NIANNCGKDD
660 670 680 690 700
LCVPDLKVTA VADREKFLLG TQDNTMLINV TVQNGGEDSY ETKLYFDVPQ
710 720 730 740 750
GFEYGGIESV GGDGSKSAPA CSPTSDEPDS DGKWTFACDL GNPLPANKVV
760 770 780 790 800
SSVVRVTASS DKPPLAPISI NAHVNSSNDE EAHTVADNKV TFTIPVDFKN
810 820 830 840 850
QLSLNGRSNP EQVDFSMTNK TRVDAFDDNE IGPVVSHLYQ ISNRGPSEVD
860 870 880 890 900
SATLDIFWPS FSTEGGHLLY IITEPVVNPP NKGRCRVKQL QNVNPLNLRI
910 920 930 940 950
TNEHVPTEPP VAKTPNEYSR EEDDESYEDE TTTQSQSTRH QSTQHQTHHQ
960 970 980 990 1000
SGPVHVYEKD EEKIRQNTGN WQYVEDKKKK GDYEYIPDDQ EYDGDDFEEE
1010 1020 1030 1040 1050
DDEDFDRAGS KRVKRNPTPK KKKKGGEHRG EPRSDKARFS DLREAVKLSK
1060 1070 1080 1090 1100
EAGGVVDYKG PLSRASVDCN SLRCTHIECD IYDLKEDEFV LVEIFSRLYT
1110 1120 1130 1140 1150
NTLVDEKNPG GDISSLALAR VTSTKYNLPH KPTLITAVST NMNAIASEEG
1160 1170 1180 1190 1200
RDLPWWLYLL AILIGLAILI LLILLLWRCG FFKRNRPPTE HAELRADRQP
1210 1220
NAQYADSQSR YTSQDQYNQG RHGQML
Length:1,226
Mass (Da):135,940
Last modified:February 1, 1994 - v1
Checksum:iB9169AD75B88901D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA Translation: CCD71234.1
PIRiS44824
RefSeqiNP_498948.1, NM_066547.3
UniGeneiCel.10427

Genome annotation databases

EnsemblMetazoaiF54F2.1; F54F2.1; WBGene00003929
GeneIDi176240
KEGGicel:CELE_F54F2.1
UCSCiF54F2.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiPAT2_CAEEL
AccessioniPrimary (citable) accession number: P34446
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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