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Protein

Abnormal embryogenesis protein 30

Gene

emb-30

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (By similarity). The APC/C complex acts by mediating ubiquination and subsequent degradation of target proteins (By similarity). Developmental role in early embryogenesis and the metaphase to anaphase transition in oocyte and spermatocyte meiosis and mitosis in somatic and germ cells (PubMed:10749938, PubMed:11134076, PubMed:17237515). Required for embryonic anterior-posterior axis formation (PubMed:11832245). Plays a role in regulating the abundance of glr-1 receptors in postmitotic neurons, which may in turn control animal locomotion (PubMed:15556870). Involved in regulating GABA neurotransmitter release at neuromuscular junctions in GABA motor neurons (PubMed:24321454).By similarity6 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis, Neurotransmitter transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Abnormal embryogenesis protein 30
Gene namesi
Name:emb-30Imported
ORF Names:F54C8.3Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF54C8.3; CE32676; WBGene00001284; emb-30.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi46 – 461L → W in TN481. 1 Publication
Mutagenesisi223 – 2231A → V in TN478. 1 Publication
Mutagenesisi224 – 2241G → D in TN479. 1 Publication
Mutagenesisi265 – 2651T → I in h1962ts; temperature sensitive mutant that can be maintained at 20 degrees Celsius, but that results in the production of unfertilized eggs and therefore sterility at 25 degrees Celsius. 1 Publication
Mutagenesisi360 – 3601E → K in G53. 1 Publication
Mutagenesisi480 – 4801M → I in h1959ts; temperature sensitive mutant that can be maintained at 20 degrees Celsius, but that results in embryonic lethality at 25 degrees Celsius. 1 Publication
Mutagenesisi483 – 4831E → K in AX69. 1 Publication
Mutagenesisi564 – 5641L → P in TN494. 1 Publication
Mutagenesisi701 – 7011G → R in TN377. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10271027Abnormal embryogenesis protein 30CuratedPRO_0000050959Add
BLAST

Proteomic databases

EPDiP34441.
PaxDbiP34441.
PRIDEiP34441.

Interactioni

Subunit structurei

The APC/C is probably composed of at least 12 subunits: apc-2, apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1, mat-2, mat-3, such-1 and gfi-3.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
pal-1P347662EBI-311903,EBI-311911

Protein-protein interaction databases

BioGridi41519. 15 interactions.
IntActiP34441. 3 interactions.
MINTiMINT-1039095.
STRINGi6239.F54C8.3.

Structurei

3D structure databases

ProteinModelPortaliP34441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 6548WD 1Add
BLAST
Repeati70 – 10940WD 2Add
BLAST

Domaini

The C-terminus is specifically required for completion of oocyte meiotic divisions.

Sequence similaritiesi

Belongs to the APC4 family.Curated
Contains 2 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410JM1R. Eukaryota.
ENOG411130M. LUCA.
HOGENOMiHOG000019161.
InParanoidiP34441.
OMAiMMQELGH.
OrthoDBiEOG7S21XB.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024789. APC4.
IPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13260. PTHR13260. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.

Sequencei

Sequence statusi: Complete.

P34441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSIVKTQI LNNRRTFRTP FKVSSMCFSS QNDLIALGSK TGEILLKRTS
60 70 80 90 100
WKMIWKTNIN MIQAVGTECK LDSSVSALHF SPDGRFLAAA TSKGIIHLLD
110 120 130 140 150
VETGKVRFSV KAASEKIAKL HWNCVREKPF ISNLGEFTTR IKNVEAIEGA
160 170 180 190 200
IELAETTPNI SQEEIAFVYQ RLDEDGSSFK HEDAHKESLE RTLISTETFR
210 220 230 240 250
ESLQNTILLA TDDMDSKIIV LVAGVFPYME IDISDTLLQY NQSLLMLYDM
260 270 280 290 300
HYSSAFGGVS FLATTYGPFL DCKQNELKPP GAEPKKDGQG CHTLLFNVKL
310 320 330 340 350
NINSSLWDTA LRYIRLLFGF NLYSISLETT KRNWEEQIDN LHSLFDTKTK
360 370 380 390 400
AVKIGNVLLE MLLSGSTDAA GEAFLERGLG TDGLDKIELF ATKHMPEVCR
410 420 430 440 450
IARGQLSTSA RNLCFQRCEF STSLSRYAKF IQLKDDDSFL YDEDPPAYLS
460 470 480 490 500
ESNIWLNTLE EKINILDMKT RHLGIQCLTM MQELGHLVKW ISMTKPFAKT
510 520 530 540 550
MKVNALMKIK RMNIAKILLY IVRNFIPDPE AVKDIENRLF NLKELVSKQK
560 570 580 590 600
KKDLEDKFDE VEYLYRLERI AEVERKKLIE TFHEKFRFAD LDDDDLTPLL
610 620 630 640 650
FQELDHPIDI FTDSMMEQND SSPFLEEDEG EPEQEEQKPD EEPEPEGEPQ
660 670 680 690 700
PACDLDRVGS FFEKELSQKA LEVLPKCDET FDCVLNERGS RMNSIEIQKM
710 720 730 740 750
GILRVLEDLA ATLSSPQKIH CDKAGNQKKL EVSFIYEITS TPTHQGYQDA
760 770 780 790 800
KISSVTPSYY KRHPFNQLSG MGRSIQVSVL QKNEFSSIVI VPTSDVLPED
810 820 830 840 850
ENEVNDHVEK LKRCYEFEKI DVTVDKSSAE NRPPGETDAM EVDSNELRID
860 870 880 890 900
VDLGPVVPEY DTLIELSQVH PLHHGELVLL GKFTSAQEGT GVMSQMMRSI
910 920 930 940 950
SSYPHEIPED KDVFAKNSSE DGSSEIPIET LIIHPTIQLA AYLHDDGSKI
960 970 980 990 1000
TIGDMKPEVP PIADYEEKTV RKTFRDYQRR RERYREDMGV MNMNDVQYDV
1010 1020
LVQEAMDSGR DLTDNGESDE DEEDDDI
Length:1,027
Mass (Da):117,051
Last modified:May 23, 2003 - v4
Checksum:iA3D35E7463303888
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192400 mRNA. Translation: AAF24233.1.
Z22178 Genomic DNA. Translation: CAA80159.3.
PIRiG88554.
S40745.
RefSeqiNP_499074.2. NM_066673.6.
UniGeneiCel.16966.

Genome annotation databases

EnsemblMetazoaiF54C8.3; F54C8.3; WBGene00001284.
GeneIDi176322.
KEGGicel:CELE_F54C8.3.
UCSCiF54C8.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192400 mRNA. Translation: AAF24233.1.
Z22178 Genomic DNA. Translation: CAA80159.3.
PIRiG88554.
S40745.
RefSeqiNP_499074.2. NM_066673.6.
UniGeneiCel.16966.

3D structure databases

ProteinModelPortaliP34441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41519. 15 interactions.
IntActiP34441. 3 interactions.
MINTiMINT-1039095.
STRINGi6239.F54C8.3.

Proteomic databases

EPDiP34441.
PaxDbiP34441.
PRIDEiP34441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF54C8.3; F54C8.3; WBGene00001284.
GeneIDi176322.
KEGGicel:CELE_F54C8.3.
UCSCiF54C8.3. c. elegans.

Organism-specific databases

CTDi176322.
WormBaseiF54C8.3; CE32676; WBGene00001284; emb-30.

Phylogenomic databases

eggNOGiENOG410JM1R. Eukaryota.
ENOG411130M. LUCA.
HOGENOMiHOG000019161.
InParanoidiP34441.
OMAiMMQELGH.
OrthoDBiEOG7S21XB.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi892090.
PROiP34441.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024789. APC4.
IPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13260. PTHR13260. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EMB-30: an APC4 homologue required for metaphase-to-anaphase transitions during meiosis and mitosis in Caenorhabditis elegans."
    Furuta T., Tuck S., Kirchner J., Koch B., Auty R., Kitagawa R., Rose A.M., Greenstein D.
    Mol. Biol. Cell 11:1401-1419(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LEU-46; ALA-223; GLY-224; GLU-360; GLU-483; LEU-564 AND GLY-701.
    Strain: Bristol N2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans."
    Golden A., Sadler P.L., Wallenfang M.R., Schumacher J.M., Hamill D.R., Bates G., Bowerman B., Seydoux G., Shakes D.C.
    J. Cell Biol. 151:1469-1482(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The anaphase-promoting complex and separin are required for embryonic anterior-posterior axis formation."
    Rappleye C.A., Tagawa A., Lyczak R., Bowerman B., Aroian R.V.
    Dev. Cell 2:195-206(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The anaphase-promoting complex regulates the abundance of GLR-1 glutamate receptors in the ventral nerve cord of C. elegans."
    Juo P., Kaplan J.M.
    Curr. Biol. 14:2057-2062(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Suppressors of spindle checkpoint defect (such) mutants identify new mdf-1/MAD1 interactors in Caenorhabditis elegans."
    Tarailo M., Kitagawa R., Rose A.M.
    Genetics 175:1665-1679(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF THR-265 AND MET-480.
  8. "The anaphase-promoting complex (APC) ubiquitin ligase regulates GABA transmission at the C. elegans neuromuscular junction."
    Kowalski J.R., Dube H., Touroutine D., Rush K.M., Goodwin P.R., Carozza M., Didier Z., Francis M.M., Juo P.
    Mol. Cell. Neurosci. 58:62-75(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiEMB30_CAEEL
AccessioniPrimary (citable) accession number: P34441
Secondary accession number(s): Q9U4A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 23, 2003
Last modified: May 11, 2016
This is version 115 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.