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Protein

Abnormal embryogenesis protein 30

Gene

emb-30

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (By similarity). The APC/C complex acts by mediating ubiquination and subsequent degradation of target proteins (By similarity). Developmental role in early embryogenesis and the metaphase to anaphase transition in oocyte and spermatocyte meiosis and mitosis in somatic and germ cells (PubMed:10749938, PubMed:12498686, PubMed:11134076, PubMed:17237515). Required for embryonic anterior-posterior axis formation (PubMed:11832245). Negatively regulates ify-1 protein levels during meiosis I (PubMed:12498686). Plays a role in regulating the abundance of glr-1 receptors in postmitotic neurons, which may in turn control animal locomotion (PubMed:15556870). Involved in regulating GABA neurotransmitter release at neuromuscular junctions in GABA motor neurons (PubMed:24321454).By similarity7 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: InterPro
  • anterior/posterior axis specification, embryo Source: WormBase
  • asymmetric cell division Source: UniProtKB
  • metaphase/anaphase transition of meiosis I Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • neurotransmitter transport Source: UniProtKB-KW
  • polarity specification of anterior/posterior axis Source: UniProtKB
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of mitotic metaphase/anaphase transition Source: InterPro

Keywordsi

Molecular functionDevelopmental protein
Biological processCell cycle, Cell division, Meiosis, Mitosis, Neurotransmitter transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Abnormal embryogenesis protein 30
Gene namesi
Name:emb-30Imported
ORF Names:F54C8.3Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF54C8.3; CE32676; WBGene00001284; emb-30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46L → W in TN481. 1 Publication1
Mutagenesisi223A → V in TN478. 1 Publication1
Mutagenesisi224G → D in TN479. 1 Publication1
Mutagenesisi265T → I in h1962ts; temperature sensitive mutant that can be maintained at 20 degrees Celsius, but that results in the production of unfertilized eggs and therefore sterility at 25 degrees Celsius. 1 Publication1
Mutagenesisi360E → K in G53. 1 Publication1
Mutagenesisi480M → I in h1959ts; temperature sensitive mutant that can be maintained at 20 degrees Celsius, but that results in embryonic lethality at 25 degrees Celsius. 1 Publication1
Mutagenesisi483E → K in AX69. 1 Publication1
Mutagenesisi564L → P in TN494. 1 Publication1
Mutagenesisi701G → R in TN377. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509591 – 1027Abnormal embryogenesis protein 30CuratedAdd BLAST1027

Proteomic databases

EPDiP34441.
PaxDbiP34441.
PeptideAtlasiP34441.
PRIDEiP34441.

Expressioni

Gene expression databases

BgeeiWBGene00001284.

Interactioni

Subunit structurei

The APC/C is probably composed of at least 12 subunits: apc-2, apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1, mat-2, mat-3, such-1 and gfi-3.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
pal-1P347662EBI-311903,EBI-311911

Protein-protein interaction databases

BioGridi41519. 15 interactors.
IntActiP34441. 3 interactors.
MINTiMINT-1039095.
STRINGi6239.F54C8.3.

Structurei

3D structure databases

ProteinModelPortaliP34441.
SMRiP34441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 65WD 1Add BLAST48
Repeati70 – 109WD 2Add BLAST40

Domaini

The C-terminus is specifically required for completion of oocyte meiotic divisions.

Sequence similaritiesi

Belongs to the APC4 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410JM1R. Eukaryota.
ENOG411130M. LUCA.
HOGENOMiHOG000019161.
InParanoidiP34441.
OMAiMMQELGH.
OrthoDBiEOG091G03I6.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR024789. APC4.
IPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR036322. WD40_repeat_dom_sf.
PANTHERiPTHR13260. PTHR13260. 1 hit.
PfamiView protein in Pfam
PF12894. ANAPC4_WD40. 1 hit.
SUPFAMiSSF50978. SSF50978. 1 hit.

Sequencei

Sequence statusi: Complete.

P34441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSIVKTQI LNNRRTFRTP FKVSSMCFSS QNDLIALGSK TGEILLKRTS
60 70 80 90 100
WKMIWKTNIN MIQAVGTECK LDSSVSALHF SPDGRFLAAA TSKGIIHLLD
110 120 130 140 150
VETGKVRFSV KAASEKIAKL HWNCVREKPF ISNLGEFTTR IKNVEAIEGA
160 170 180 190 200
IELAETTPNI SQEEIAFVYQ RLDEDGSSFK HEDAHKESLE RTLISTETFR
210 220 230 240 250
ESLQNTILLA TDDMDSKIIV LVAGVFPYME IDISDTLLQY NQSLLMLYDM
260 270 280 290 300
HYSSAFGGVS FLATTYGPFL DCKQNELKPP GAEPKKDGQG CHTLLFNVKL
310 320 330 340 350
NINSSLWDTA LRYIRLLFGF NLYSISLETT KRNWEEQIDN LHSLFDTKTK
360 370 380 390 400
AVKIGNVLLE MLLSGSTDAA GEAFLERGLG TDGLDKIELF ATKHMPEVCR
410 420 430 440 450
IARGQLSTSA RNLCFQRCEF STSLSRYAKF IQLKDDDSFL YDEDPPAYLS
460 470 480 490 500
ESNIWLNTLE EKINILDMKT RHLGIQCLTM MQELGHLVKW ISMTKPFAKT
510 520 530 540 550
MKVNALMKIK RMNIAKILLY IVRNFIPDPE AVKDIENRLF NLKELVSKQK
560 570 580 590 600
KKDLEDKFDE VEYLYRLERI AEVERKKLIE TFHEKFRFAD LDDDDLTPLL
610 620 630 640 650
FQELDHPIDI FTDSMMEQND SSPFLEEDEG EPEQEEQKPD EEPEPEGEPQ
660 670 680 690 700
PACDLDRVGS FFEKELSQKA LEVLPKCDET FDCVLNERGS RMNSIEIQKM
710 720 730 740 750
GILRVLEDLA ATLSSPQKIH CDKAGNQKKL EVSFIYEITS TPTHQGYQDA
760 770 780 790 800
KISSVTPSYY KRHPFNQLSG MGRSIQVSVL QKNEFSSIVI VPTSDVLPED
810 820 830 840 850
ENEVNDHVEK LKRCYEFEKI DVTVDKSSAE NRPPGETDAM EVDSNELRID
860 870 880 890 900
VDLGPVVPEY DTLIELSQVH PLHHGELVLL GKFTSAQEGT GVMSQMMRSI
910 920 930 940 950
SSYPHEIPED KDVFAKNSSE DGSSEIPIET LIIHPTIQLA AYLHDDGSKI
960 970 980 990 1000
TIGDMKPEVP PIADYEEKTV RKTFRDYQRR RERYREDMGV MNMNDVQYDV
1010 1020
LVQEAMDSGR DLTDNGESDE DEEDDDI
Length:1,027
Mass (Da):117,051
Last modified:May 23, 2003 - v4
Checksum:iA3D35E7463303888
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF192400 mRNA. Translation: AAF24233.1.
Z22178 Genomic DNA. Translation: CAA80159.3.
PIRiG88554.
S40745.
RefSeqiNP_499074.2. NM_066673.6.
UniGeneiCel.16966.

Genome annotation databases

EnsemblMetazoaiF54C8.3; F54C8.3; WBGene00001284.
GeneIDi176322.
KEGGicel:CELE_F54C8.3.
UCSCiF54C8.3. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiEMB30_CAEEL
AccessioniPrimary (citable) accession number: P34441
Secondary accession number(s): Q9U4A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 23, 2003
Last modified: November 22, 2017
This is version 127 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families