P34385 (MCCB_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Short name=MCCase subunit beta
EC=6.4.1.4

Alternative name(s):
3-methylcrotonyl-CoA carboxylase 2
3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit
3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta

Gene names

ORF Names:

F02A9.4

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.
Pathway	Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the AccD/PCCB family. Contains 1 carboxyltransferase domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	leucine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from direct assay PubMed 21611156. Source: WormBase mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methylcrotonoyl-CoA carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P34385-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: P34384-1)

The sequence of this isoform can be found in the external entry P34384.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 61	61	Mitochondrion Potential
	Chain	62 – 608	547	Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial		PRO_0000199804
Regions
	Domain	100 – 602	503	Carboxyltransferase
	Region	388 – 417	30	Acyl-CoA binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 2502FB70D987B6E0	FASTA	608	66,524
	10 20 30 40 50 60 MFRHVAQNLG SRNTSIQSYR LLRTRWERGY LKDLYHRRQI LGADPAISRS SYPNCSVQVR 70 80 90 100 110 120 NHHCSADKST LQWAPIKTSI DNSSDDFAAN TAEMKVLVED LKAKISKIEQ AGGEKAVKLH 130 140 150 160 170 180 RSRGKMLARE RIDGIVDAGS PFIEFSQLAG YEMYGKEEVP SGGILTGVGI VSGRVCVIVA 190 200 210 220 230 240 NDATVKGGTY YPITVKKHLR AQEIARENKL PCIYLVDSGG ANLPRQADIF ADSQHFGRIF 250 260 270 280 290 300 YNQATMSSEG IPQLAVVMGS CTAGGAYVPA MSDQAIIVKG TGTVFLGGPP LVKAATGEEI 310 320 330 340 350 360 SAEELGGADL HCGESGVTDY YAHNDKHALY LARSCIAGLP PVEEHMTFNP NADEPLYPAE 370 380 390 400 410 420 EIYGIVGSNL KKTYDVREVI ARIVDGSRFH EFKERYGETL VTGFATIYGQ RVGILANNGV 430 440 450 460 470 480 LFAESAMKGS HFIELCCQRK IPLLFLQNIT GFMVGRDAEA GGIAKHGAKL VTAVACAKVP 490 500 510 520 530 540 KITVLVGGSY GAGNYGMCGR GYSPRYVFMW PNSRISVMGG EQAANVLSTV QKEKKKREGA 550 560 570 580 590 600 DWTDQQDLEL RKPVEEKFEK EGHPYFASAR LWDDGVIDPK DTRKVLGLAF QSTLQKPIPE TKFGVFRM
	Isoform b [UniParc]. See P34384.		–	–

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z19555 Genomic DNA. Translation: CAA79618.1.
PIR	S28313.
RefSeq	NP_499013.1. NM_066612.4.
3D structure databases
ProteinModelPortal	P34385.
SMR	P34385. Positions 74-608.
ModBase	Search...
Proteomic databases
PaxDb	P34385.
PRIDE	P34385.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F02A9.4a; F02A9.4a; F02A9.4.
GeneID	176285.
KEGG	cel:CELE_F02A9.4.
UCSC	F02A9.4b. c. elegans.
Organism-specific databases
CTD	176285.
WormBase	F02A9.4a; CE00136; WBGene00008514.
Phylogenomic databases
eggNOG	COG4799.
GeneTree	ENSGT00530000063337.
HOGENOM	HOG000218692.
InParanoid	P34385.
OMA	KFGIFRM.
Enzyme and pathway databases
UniPathway	UPA00363; UER00861.
Gene expression databases
ArrayExpress	P34385.
Family and domain databases
InterPro	IPR000022. Carboxyl_trans. IPR011763. COA_CT_C. IPR011762. COA_CT_N. [Graphical view]
Pfam	PF01039. Carboxyl_trans. 1 hit. [Graphical view]
PROSITE	PS50989. COA_CT_CTER. 1 hit. PS50980. COA_CT_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	891926.

Entry information

Entry name

MCCB_CAEEL

Accession

Primary (citable) accession number: P34385

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project