Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adenylate kinase

Gene

let-754

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571AMPUniRule annotation
Binding sitei62 – 621AMPUniRule annotation
Binding sitei118 – 1181AMPUniRule annotation
Binding sitei153 – 1531ATPUniRule annotation
Binding sitei186 – 1861AMPUniRule annotation
Binding sitei197 – 1971AMPUniRule annotation
Binding sitei225 – 2251ATP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 416ATPUniRule annotation
Nucleotide bindingi83 – 853AMPUniRule annotation
Nucleotide bindingi111 – 1144AMPUniRule annotation
Nucleotide bindingi162 – 1632ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • ADP biosynthetic process Source: UniProtKB-HAMAP
  • AMP metabolic process Source: UniProtKB-HAMAP
  • ATP metabolic process Source: UniProtKB-HAMAP
  • negative regulation of protein kinase C signaling Source: WormBase
  • regulation of establishment of cell polarity Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinaseUniRule annotation (EC:2.7.4.3UniRule annotation)
Alternative name(s):
ATP-AMP transphosphorylaseUniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate kinase cytosolic and mitochondrialUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
Lethal protein 754
Gene namesi
Name:let-754UniRule annotation
ORF Names:C29E4.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC29E4.8; CE29198; WBGene00002879; let-754.

Subcellular locationi

  • Cytoplasmcytosol UniRule annotation
  • Mitochondrion intermembrane space UniRule annotation

  • Note: Predominantly mitochondrial.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Adenylate kinasePRO_0000158933Add
BLAST

Proteomic databases

EPDiP34346.
PaxDbiP34346.

Expressioni

Gene expression databases

BgeeiWBGene00002879.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi41325. 1 interaction.
STRINGi6239.C29E4.8.1.

Structurei

3D structure databases

ProteinModelPortaliP34346.
SMRiP34346. Positions 26-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni56 – 8530NMPbindUniRule annotationAdd
BLAST
Regioni152 – 18938LIDUniRule annotationAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOGENOMiHOG000238772.
InParanoidiP34346.
KOiK00939.
OMAiRRLKCSQ.
OrthoDBiEOG091G06BH.
PhylomeDBiP34346.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03168. Adenylate_kinase_AK2. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028587. AK2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P34346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVPKTQQSA QPATQSAGPT ETLARGIRAI FIGPPGSGKG TQAPAFAQKY
60 70 80 90 100
FSCHLATGDL LRAEVASGSE FGKELKATMD AGKLVSDEVV CKLIEQKLEK
110 120 130 140 150
PECKYGFILD GFPRTSGQAE KLDEILERRK TPLDTVVEFN IADDLLVRRI
160 170 180 190 200
TGRLFHIASG RSYHLEFKPP KVPMKDDLTG EPLIRRSDDN EETLRKRLVQ
210 220 230 240 250
YHQMTVPLVD YYKKHGVHVA VDAAKPMTDV KAHIDQVFAK FTQKKERVSF

V
Length:251
Mass (Da):27,925
Last modified:May 15, 2002 - v2
Checksum:iB5BA73D430CD2D17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080706 Genomic DNA. Translation: CCD66007.1.
PIRiS44766.
RefSeqiNP_498730.1. NM_066329.5.
UniGeneiCel.17325.

Genome annotation databases

EnsemblMetazoaiC29E4.8.1; C29E4.8.1; WBGene00002879.
C29E4.8.2; C29E4.8.2; WBGene00002879.
GeneIDi176118.
KEGGicel:CELE_C29E4.8.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080706 Genomic DNA. Translation: CCD66007.1.
PIRiS44766.
RefSeqiNP_498730.1. NM_066329.5.
UniGeneiCel.17325.

3D structure databases

ProteinModelPortaliP34346.
SMRiP34346. Positions 26-242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41325. 1 interaction.
STRINGi6239.C29E4.8.1.

Proteomic databases

EPDiP34346.
PaxDbiP34346.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC29E4.8.1; C29E4.8.1; WBGene00002879.
C29E4.8.2; C29E4.8.2; WBGene00002879.
GeneIDi176118.
KEGGicel:CELE_C29E4.8.

Organism-specific databases

CTDi176118.
WormBaseiC29E4.8; CE29198; WBGene00002879; let-754.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOGENOMiHOG000238772.
InParanoidiP34346.
KOiK00939.
OMAiRRLKCSQ.
OrthoDBiEOG091G06BH.
PhylomeDBiP34346.

Enzyme and pathway databases

ReactomeiR-CEL-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

PROiP34346.

Gene expression databases

BgeeiWBGene00002879.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03168. Adenylate_kinase_AK2. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028587. AK2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD2_CAEEL
AccessioniPrimary (citable) accession number: P34346
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 15, 2002
Last modified: September 7, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.